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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 397 |
| AccB1I | GGYRCC | 1 cut(s) | 397 |
| AccI | GTMKAC | 1 cut(s) | 376 |
| AccII | CGCG | 1 cut(s) | 358 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AcuI | CTGAAG | 1 cut(s) | 558 |
| AfaI | GTAC | 4 cut(s) | 111, 364, 399, 405 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 561, 562 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 2 cut(s) | 524, 557 |
| AluBI | AGCT | 2 cut(s) | 77, 199 |
| AluI | AGCT | 2 cut(s) | 77, 199 |
| Alw26I | GTCTC | 1 cut(s) | 137 |
| AoxI | GGCC | 5 cut(s) | 163, 258, 264, 451, 528 |
| ApaI | GGGCCC | 1 cut(s) | 455 |
| ApeKI | GCWGC | 5 cut(s) | 31, 74, 137, 156, 521 |
| Asp718I | GGTACC | 1 cut(s) | 397 |
| AspLEI | GCGC | 1 cut(s) | 319 |
| AspS9I | GGNCC | 8 cut(s) | 40, 164, 172, 178, 265, 451, 452, 528 |
| AsuC2I | CCSGG | 3 cut(s) | 153, 176, 518 |
| AsuHPI | GGTGA | 2 cut(s) | 289, 540 |
| AvaII | GGWCC | 3 cut(s) | 40, 172, 178 |
| BaeGI | GKGCMC | 1 cut(s) | 455 |
| BanI | GGYRCC | 1 cut(s) | 397 |
| BanII | GRGCYC | 1 cut(s) | 455 |
| BbvI | GCAGC | 5 cut(s) | 43, 86, 149, 168, 533 |
| BccI | CCATC | 2 cut(s) | 133, 442 |
| BciT130I | CCWGG | 2 cut(s) | 526, 559 |
| BcnI | CCSGG | 3 cut(s) | 153, 176, 518 |
| BcoDI | GTCTC | 1 cut(s) | 137 |
| BfaI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| BfoI | RGCGCY | 1 cut(s) | 320 |
| BisI | GCNGC | 6 cut(s) | 32, 75, 138, 157, 186, 522 |
| BlpI | GCTNAGC | 1 cut(s) | 27 |
| BlsI | GCNGC | 6 cut(s) | 33, 76, 139, 158, 187, 523 |
| BmcAI | AGTACT | 1 cut(s) | 364 |
| Bme1390I | CCNGG | 5 cut(s) | 153, 176, 518, 526, 559 |
| Bme18I | GGWCC | 3 cut(s) | 40, 172, 178 |
| BmgT120I | GGNCC | 8 cut(s) | 40, 164, 172, 178, 265, 451, 452, 528 |
| BmiI | GGNNCC | 3 cut(s) | 179, 399, 453 |
| BmrFI | CCNGG | 5 cut(s) | 153, 176, 518, 526, 559 |
| BmsI | GCATC | 1 cut(s) | 408 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 3 cut(s) | 153, 176, 518 |
| BsaI | GGTCTC | 1 cut(s) | 137 |
| BsaJI | CCNNGG | 3 cut(s) | 261, 525, 557 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 561, 562 |
| Bse1I | ACTGG | 2 cut(s) | 43, 407 |
| BseBI | CCWGG | 2 cut(s) | 526, 559 |
| BseDI | CCNNGG | 3 cut(s) | 261, 525, 557 |
| BseGI | GGATG | 1 cut(s) | 125 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 561, 562 |
| BseMII | CTCAG | 2 cut(s) | 41, 385 |
| BseNI | ACTGG | 2 cut(s) | 43, 407 |
| BseRI | GAGGAG | 1 cut(s) | 450 |
| BseSI | GKGCMC | 1 cut(s) | 455 |
| BseXI | GCAGC | 5 cut(s) | 43, 86, 149, 168, 533 |
| BseYI | CCCAGC | 1 cut(s) | 147 |
| Bsh1236I | CGCG | 1 cut(s) | 358 |
| BshFI | GGCC | 5 cut(s) | 165, 260, 266, 453, 530 |
| BshNI | GGYRCC | 1 cut(s) | 397 |
| BsiSI | CCGG | 3 cut(s) | 152, 175, 518 |
| BslFI | GGGAC | 1 cut(s) | 191 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 561, 562 |
| BsmAI | GTCTC | 1 cut(s) | 137 |
| BsmFI | GGGAC | 1 cut(s) | 191 |
| BsmI | GAATGC | 1 cut(s) | 239 |
| BsnI | GGCC | 5 cut(s) | 165, 260, 266, 453, 530 |
| Bso31I | GGTCTC | 1 cut(s) | 137 |
| Bsp120I | GGGCCC | 1 cut(s) | 451 |
| Bsp1286I | GDGCHC | 1 cut(s) | 455 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 27 |
| BspANI | GGCC | 5 cut(s) | 165, 260, 266, 453, 530 |
| BspCNI | CTCAG | 2 cut(s) | 40, 384 |
| BspFNI | CGCG | 1 cut(s) | 358 |
| BspLI | GGNNCC | 3 cut(s) | 179, 399, 453 |
| BspT107I | GGYRCC | 1 cut(s) | 397 |
| BspTNI | GGTCTC | 1 cut(s) | 137 |
| BsrI | ACTGG | 2 cut(s) | 43, 407 |
| BssECI | CCNNGG | 3 cut(s) | 261, 525, 557 |
| Bst2UI | CCWGG | 2 cut(s) | 526, 559 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 427 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 201, 350, 455 |
| BstDEI | CTNAG | 2 cut(s) | 27, 371 |
| BstEII | GGTNACC | 1 cut(s) | 277 |
| BstF5I | GGATG | 1 cut(s) | 125 |
| BstFNI | CGCG | 1 cut(s) | 358 |
| BstH2I | RGCGCY | 1 cut(s) | 320 |
| BstHHI | GCGC | 1 cut(s) | 319 |
| BstMAI | GTCTC | 1 cut(s) | 137 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 162, 323, 338, 427, 463, 527 |
| BstNI | CCWGG | 2 cut(s) | 526, 559 |
| BstPI | GGTNACC | 1 cut(s) | 277 |
| BstSCI | CCNGG | 5 cut(s) | 151, 174, 516, 524, 557 |
| BstSLI | GKGCMC | 1 cut(s) | 455 |
| BstUI | CGCG | 1 cut(s) | 358 |
| BstV1I | GCAGC | 5 cut(s) | 43, 86, 149, 168, 533 |
| BsuRI | GGCC | 5 cut(s) | 165, 260, 266, 453, 530 |
| BtsCI | GGATG | 1 cut(s) | 125 |
| BtsI | GCAGTG | 1 cut(s) | 166 |
| BtsIMutI | CAGTG | 1 cut(s) | 166 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 201, 350, 455 |
| CfoI | GCGC | 1 cut(s) | 319 |
| Cfr13I | GGNCC | 8 cut(s) | 40, 164, 172, 178, 265, 451, 452, 528 |
| Csp6I | GTAC | 4 cut(s) | 110, 363, 398, 404 |
| CviAII | CATG | 2 cut(s) | 220, 283 |
| CviQI | GTAC | 4 cut(s) | 110, 363, 398, 404 |
| DdeI | CTNAG | 2 cut(s) | 27, 371 |
| EciI | GGCGGA | 1 cut(s) | 405 |
| Eco24I | GRGCYC | 1 cut(s) | 455 |
| Eco31I | GGTCTC | 1 cut(s) | 137 |
| Eco47I | GGWCC | 3 cut(s) | 40, 172, 178 |
| Eco57I | CTGAAG | 1 cut(s) | 558 |
| Eco91I | GGTNACC | 1 cut(s) | 277 |
| EcoO109I | RGGNCCY | 1 cut(s) | 178 |
| EcoO65I | GGTNACC | 1 cut(s) | 277 |
| EcoRII | CCWGG | 2 cut(s) | 524, 557 |
| EcoT38I | GRGCYC | 1 cut(s) | 455 |
| FaeI | CATG | 2 cut(s) | 223, 286 |
| FaiI | YATR | 8 cut(s) | 191, 195, 221, 251, 284, 386, 413, 569 |
| FaqI | GGGAC | 1 cut(s) | 191 |
| FatI | CATG | 2 cut(s) | 219, 282 |
| FauI | CCCGC | 4 cut(s) | 8, 462, 476, 555 |
| FblI | GTMKAC | 1 cut(s) | 376 |
| Fnu4HI | GCNGC | 6 cut(s) | 32, 75, 138, 157, 186, 522 |
| FokI | GGATG | 1 cut(s) | 112 |
| FriOI | GRGCYC | 1 cut(s) | 455 |
| Fsp4HI | GCNGC | 6 cut(s) | 32, 75, 138, 157, 186, 522 |
| FspBI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| GlaI | GCGC | 1 cut(s) | 318 |
| GluI | GCNGC | 6 cut(s) | 32, 75, 138, 157, 186, 522 |
| GsaI | CCCAGC | 1 cut(s) | 151 |
| HaeII | RGCGCY | 1 cut(s) | 320 |
| HaeIII | GGCC | 5 cut(s) | 165, 260, 266, 453, 530 |
| HapII | CCGG | 3 cut(s) | 152, 175, 518 |
| HhaI | GCGC | 1 cut(s) | 319 |
| Hin1II | CATG | 2 cut(s) | 223, 286 |
| Hin6I | GCGC | 1 cut(s) | 317 |
| HinP1I | GCGC | 1 cut(s) | 317 |
| HincII | GTYRAC | 2 cut(s) | 85, 377 |
| HindII | GTYRAC | 2 cut(s) | 85, 377 |
| HinfI | GANTC | 2 cut(s) | 369, 494 |
| HpaII | CCGG | 3 cut(s) | 152, 175, 518 |
| HphI | GGTGA | 2 cut(s) | 289, 540 |
| Hpy166II | GTNNAC | 5 cut(s) | 22, 85, 97, 110, 377 |
| Hpy8I | GTNNAC | 5 cut(s) | 22, 85, 97, 110, 377 |
| Hpy99I | CGWCG | 1 cut(s) | 305 |
| HpyAV | CCTTC | 1 cut(s) | 533 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 2 cut(s) | 137, 421 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 162, 323, 338, 427, 463, 527 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 371 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| Hsp92II | CATG | 2 cut(s) | 223, 286 |
| HspAI | GCGC | 1 cut(s) | 317 |
| KpnI | GGTACC | 1 cut(s) | 401 |
| Lsp1109I | GCAGC | 5 cut(s) | 43, 86, 149, 168, 533 |
| LweI | GCATC | 1 cut(s) | 408 |
| MaeI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MaeIII | GTNAC | 1 cut(s) | 277 |
| MhlI | GDGCHC | 1 cut(s) | 455 |
| MluCI | AATT | 2 cut(s) | 305, 544 |
| MlyI | GAGTC | 1 cut(s) | 363 |
| MmeI | TCCRAC | 1 cut(s) | 153 |
| MnlI | CCTC | 6 cut(s) | 191, 224, 256, 468, 471, 481 |
| MseI | TTAA | 2 cut(s) | 102, 308 |
| MslI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| MspA1I | CMGCKG | 1 cut(s) | 140 |
| MspI | CCGG | 3 cut(s) | 152, 175, 518 |
| MspR9I | CCNGG | 5 cut(s) | 153, 176, 518, 526, 559 |
| Mva1269I | GAATGC | 1 cut(s) | 239 |
| MvaI | CCWGG | 2 cut(s) | 526, 559 |
| MvnI | CGCG | 1 cut(s) | 358 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 162, 323, 338, 427, 463, 527 |
| NciI | CCSGG | 3 cut(s) | 153, 176, 518 |
| NlaIII | CATG | 2 cut(s) | 223, 286 |
| NlaIV | GGNNCC | 3 cut(s) | 179, 399, 453 |
| NmeAIII | GCCGAG | 1 cut(s) | 286 |
| NmuCI | GTSAC | 1 cut(s) | 277 |
| PctI | GAATGC | 1 cut(s) | 239 |
| PfeI | GAWTC | 1 cut(s) | 494 |
| PflFI | GACNNNGTC | 1 cut(s) | 372 |
| PfoI | TCCNGGA | 1 cut(s) | 174 |
| PkrI | GCNGC | 6 cut(s) | 33, 76, 139, 158, 187, 523 |
| PleI | GAGTC | 1 cut(s) | 363 |
| PpsI | GAGTC | 1 cut(s) | 363 |
| PpuMI | RGGWCCY | 1 cut(s) | 178 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 178 |
| Psp6I | CCWGG | 2 cut(s) | 524, 557 |
| PspEI | GGTNACC | 1 cut(s) | 277 |
| PspFI | CCCAGC | 1 cut(s) | 147 |
| PspGI | CCWGG | 2 cut(s) | 524, 557 |
| PspN4I | GGNNCC | 3 cut(s) | 179, 399, 453 |
| PspOMI | GGGCCC | 1 cut(s) | 451 |
| PspPI | GGNCC | 8 cut(s) | 40, 164, 172, 178, 265, 451, 452, 528 |
| PspPPI | RGGWCCY | 1 cut(s) | 178 |
| PsyI | GACNNNGTC | 1 cut(s) | 372 |
| RsaI | GTAC | 4 cut(s) | 111, 364, 399, 405 |
| RsaNI | GTAC | 4 cut(s) | 110, 363, 398, 404 |
| RseI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| SalI | GTCGAC | 1 cut(s) | 375 |
| SaqAI | TTAA | 2 cut(s) | 102, 308 |
| SatI | GCNGC | 6 cut(s) | 32, 75, 138, 157, 186, 522 |
| Sau96I | GGNCC | 8 cut(s) | 40, 164, 172, 178, 265, 451, 452, 528 |
| ScaI | AGTACT | 1 cut(s) | 364 |
| SchI | GAGTC | 1 cut(s) | 363 |
| ScrFI | CCNGG | 5 cut(s) | 153, 176, 518, 526, 559 |
| SduI | GDGCHC | 1 cut(s) | 455 |
| SfaNI | GCATC | 1 cut(s) | 408 |
| SinI | GGWCC | 3 cut(s) | 40, 172, 178 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| Sse9I | AATT | 2 cut(s) | 305, 544 |
| SspMI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| StyD4I | CCNGG | 5 cut(s) | 151, 174, 516, 524, 557 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 2 cut(s) | 300, 376 |
| TasI | AATT | 2 cut(s) | 305, 544 |
| TatI | WGTACW | 2 cut(s) | 109, 362 |
| TauI | GCSGC | 1 cut(s) | 188 |
| TfiI | GAWTC | 1 cut(s) | 494 |
| Tru1I | TTAA | 2 cut(s) | 102, 308 |
| Tru9I | TTAA | 2 cut(s) | 102, 308 |
| TscAI | CASTG | 1 cut(s) | 166 |
| TseFI | GTSAC | 1 cut(s) | 277 |
| TseI | GCWGC | 5 cut(s) | 31, 74, 137, 156, 521 |
| Tsp45I | GTSAC | 1 cut(s) | 277 |
| TspRI | CASTG | 1 cut(s) | 166 |
| Tth111I | GACNNNGTC | 1 cut(s) | 372 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 172, 178 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 565 |
| XmiI | GTMKAC | 1 cut(s) | 376 |
| XspI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| ZrmI | AGTACT | 1 cut(s) | 364 |