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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 600 |
| Acc36I | ACCTGC | 2 cut(s) | 442, 600 |
| Acc65I | GGTACC | 2 cut(s) | 427, 454 |
| AccB1I | GGYRCC | 2 cut(s) | 427, 454 |
| AccBSI | CCGCTC | 1 cut(s) | 552 |
| AccI | GTMKAC | 1 cut(s) | 406 |
| AccII | CGCG | 2 cut(s) | 59, 267 |
| AciI | CCGC | 8 cut(s) | 48, 57, 216, 239, 432, 458, 527, 550 |
| AclI | AACGTT | 1 cut(s) | 84 |
| AclWI | GGATC | 1 cut(s) | 216 |
| AcoI | YGGCCR | 1 cut(s) | 309 |
| AcuI | CTGAAG | 2 cut(s) | 48, 359 |
| AfaI | GTAC | 4 cut(s) | 394, 429, 456, 542 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 124, 250, 416, 602, 603, 630 |
| AgsI | TTSAA | 1 cut(s) | 103 |
| AjnI | CCWGG | 5 cut(s) | 132, 181, 199, 204, 598 |
| AluBI | AGCT | 4 cut(s) | 90, 316, 475, 609 |
| AluI | AGCT | 4 cut(s) | 90, 316, 475, 609 |
| Alw21I | GWGCWC | 1 cut(s) | 97 |
| Alw44I | GTGCAC | 1 cut(s) | 93 |
| AlwI | GGATC | 1 cut(s) | 216 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 151 |
| AoxI | GGCC | 3 cut(s) | 202, 309, 634 |
| ApaI | GGGCCC | 1 cut(s) | 638 |
| ApaLI | GTGCAC | 1 cut(s) | 93 |
| Asp718I | GGTACC | 2 cut(s) | 427, 454 |
| AspLEI | GCGC | 4 cut(s) | 267, 279, 349, 373 |
| AspS9I | GGNCC | 4 cut(s) | 241, 488, 634, 635 |
| AsuHPI | GGTGA | 1 cut(s) | 492 |
| AvaII | GGWCC | 2 cut(s) | 241, 488 |
| BaeGI | GKGCMC | 2 cut(s) | 97, 638 |
| BalI | TGGCCA | 1 cut(s) | 311 |
| BanI | GGYRCC | 2 cut(s) | 427, 454 |
| BanII | GRGCYC | 1 cut(s) | 638 |
| Bbv12I | GWGCWC | 1 cut(s) | 97 |
| BccI | CCATC | 1 cut(s) | 475 |
| BciT130I | CCWGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| BciVI | GTATCC | 1 cut(s) | 130 |
| BfaI | CTAG | 3 cut(s) | 120, 479, 567 |
| BfmI | CTRYAG | 1 cut(s) | 165 |
| BfoI | RGCGCY | 1 cut(s) | 350 |
| BfuAI | ACCTGC | 2 cut(s) | 442, 600 |
| BfuI | GTATCC | 1 cut(s) | 130 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 201 |
| BisI | GCNGC | 1 cut(s) | 216 |
| BlsI | GCNGC | 1 cut(s) | 217 |
| Bme1390I | CCNGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| Bme18I | GGWCC | 2 cut(s) | 241, 488 |
| BmgT120I | GGNCC | 4 cut(s) | 241, 488, 634, 635 |
| BmiI | GGNNCC | 6 cut(s) | 129, 429, 456, 489, 635, 636 |
| BmrFI | CCNGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| BmsI | GCATC | 2 cut(s) | 389, 438 |
| BpmI | CTGGAG | 2 cut(s) | 371, 631 |
| BsaAI | YACGTR | 1 cut(s) | 648 |
| BsaJI | CCNNGG | 2 cut(s) | 132, 598 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 598, 608, 628, 638 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 124, 250, 416, 602, 603, 630 |
| Bse1I | ACTGG | 1 cut(s) | 645 |
| BseBI | CCWGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| BseDI | CCNNGG | 2 cut(s) | 132, 598 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 124, 250, 416, 602, 603, 630 |
| BseNI | ACTGG | 1 cut(s) | 645 |
| BseSI | GKGCMC | 2 cut(s) | 97, 638 |
| BseYI | CCCAGC | 1 cut(s) | 367 |
| Bsh1236I | CGCG | 2 cut(s) | 59, 267 |
| BshFI | GGCC | 3 cut(s) | 204, 311, 636 |
| BshNI | GGYRCC | 2 cut(s) | 427, 454 |
| BsiHKAI | GWGCWC | 1 cut(s) | 97 |
| BsiSI | CCGG | 1 cut(s) | 417 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 124, 250, 416, 602, 603, 630 |
| BsnI | GGCC | 3 cut(s) | 204, 311, 636 |
| Bsp120I | GGGCCC | 1 cut(s) | 634 |
| Bsp1286I | GDGCHC | 2 cut(s) | 97, 638 |
| Bsp1407I | TGTACA | 1 cut(s) | 540 |
| Bsp143I | GATC | 1 cut(s) | 208 |
| BspACI | CCGC | 8 cut(s) | 48, 57, 216, 239, 432, 458, 527, 550 |
| BspANI | GGCC | 3 cut(s) | 204, 311, 636 |
| BspFNI | CGCG | 2 cut(s) | 59, 267 |
| BspLI | GGNNCC | 6 cut(s) | 129, 429, 456, 489, 635, 636 |
| BspMAI | CTGCAG | 1 cut(s) | 169 |
| BspMI | ACCTGC | 2 cut(s) | 442, 600 |
| BspPI | GGATC | 1 cut(s) | 216 |
| BspT107I | GGYRCC | 2 cut(s) | 427, 454 |
| BsrBI | CCGCTC | 1 cut(s) | 552 |
| BsrGI | TGTACA | 1 cut(s) | 540 |
| BsrI | ACTGG | 1 cut(s) | 645 |
| BssECI | CCNNGG | 2 cut(s) | 132, 598 |
| BssMI | GATC | 1 cut(s) | 208 |
| Bst2UI | CCWGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 191, 578 |
| BstAUI | TGTACA | 1 cut(s) | 540 |
| BstBAI | YACGTR | 1 cut(s) | 648 |
| BstC8I | GCNNGC | 1 cut(s) | 380 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 248, 628 |
| BstFNI | CGCG | 2 cut(s) | 59, 267 |
| BstH2I | RGCGCY | 1 cut(s) | 350 |
| BstHHI | GCGC | 4 cut(s) | 267, 279, 349, 373 |
| BstKTI | GATC | 1 cut(s) | 211 |
| BstMBI | GATC | 1 cut(s) | 208 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 56, 173, 192, 201, 353, 368, 379, 457, 563 |
| BstNI | CCWGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| BstSCI | CCNGG | 5 cut(s) | 132, 181, 199, 204, 598 |
| BstSFI | CTRYAG | 1 cut(s) | 165 |
| BstSLI | GKGCMC | 2 cut(s) | 97, 638 |
| BstSNI | TACGTA | 1 cut(s) | 648 |
| BstUI | CGCG | 2 cut(s) | 59, 267 |
| BstX2I | RGATCY | 1 cut(s) | 208 |
| BstYI | RGATCY | 1 cut(s) | 208 |
| BsuI | GTATCC | 1 cut(s) | 130 |
| BsuRI | GGCC | 3 cut(s) | 204, 311, 636 |
| BtsI | GCAGTG | 1 cut(s) | 174 |
| BtsIMutI | CAGTG | 2 cut(s) | 174, 638 |
| BveI | ACCTGC | 2 cut(s) | 442, 600 |
| Cac8I | GCNNGC | 1 cut(s) | 380 |
| CaiI | CAGNNNCTG | 1 cut(s) | 151 |
| CfoI | GCGC | 4 cut(s) | 267, 279, 349, 373 |
| Cfr13I | GGNCC | 4 cut(s) | 241, 488, 634, 635 |
| Csp6I | GTAC | 4 cut(s) | 393, 428, 455, 541 |
| CviAII | CATG | 2 cut(s) | 124, 628 |
| CviQI | GTAC | 4 cut(s) | 393, 428, 455, 541 |
| DpnI | GATC | 1 cut(s) | 210 |
| DpnII | GATC | 1 cut(s) | 208 |
| EaeI | YGGCCR | 1 cut(s) | 309 |
| EciI | GGCGGA | 2 cut(s) | 37, 254 |
| Eco105I | TACGTA | 1 cut(s) | 648 |
| Eco24I | GRGCYC | 1 cut(s) | 638 |
| Eco47I | GGWCC | 2 cut(s) | 241, 488 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 359 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 248, 628 |
| EcoO109I | RGGNCCY | 1 cut(s) | 634 |
| EcoRII | CCWGG | 5 cut(s) | 132, 181, 199, 204, 598 |
| EcoT38I | GRGCYC | 1 cut(s) | 638 |
| FaeI | CATG | 2 cut(s) | 127, 631 |
| FaiI | YATR | 5 cut(s) | 125, 221, 225, 443, 629 |
| FatI | CATG | 2 cut(s) | 123, 627 |
| FauI | CCCGC | 2 cut(s) | 64, 439 |
| FblI | GTMKAC | 1 cut(s) | 406 |
| Fnu4HI | GCNGC | 1 cut(s) | 216 |
| FriOI | GRGCYC | 1 cut(s) | 638 |
| Fsp4HI | GCNGC | 1 cut(s) | 216 |
| FspBI | CTAG | 3 cut(s) | 120, 479, 567 |
| GlaI | GCGC | 4 cut(s) | 266, 278, 348, 372 |
| GluI | GCNGC | 1 cut(s) | 216 |
| GsaI | CCCAGC | 1 cut(s) | 371 |
| GsuI | CTGGAG | 2 cut(s) | 371, 631 |
| HaeII | RGCGCY | 1 cut(s) | 350 |
| HaeIII | GGCC | 3 cut(s) | 204, 311, 636 |
| HapII | CCGG | 1 cut(s) | 417 |
| HhaI | GCGC | 4 cut(s) | 267, 279, 349, 373 |
| Hin1II | CATG | 2 cut(s) | 127, 631 |
| Hin6I | GCGC | 4 cut(s) | 265, 277, 347, 371 |
| HinP1I | GCGC | 4 cut(s) | 265, 277, 347, 371 |
| HincII | GTYRAC | 4 cut(s) | 70, 82, 115, 407 |
| HindII | GTYRAC | 4 cut(s) | 70, 82, 115, 407 |
| HinfI | GANTC | 2 cut(s) | 327, 530 |
| HpaI | GTTAAC | 3 cut(s) | 70, 82, 115 |
| HpaII | CCGG | 1 cut(s) | 417 |
| HphI | GGTGA | 1 cut(s) | 492 |
| Hpy166II | GTNNAC | 7 cut(s) | 70, 82, 95, 115, 407, 491, 543 |
| Hpy188I | TCNGA | 1 cut(s) | 472 |
| Hpy188III | TCNNGA | 1 cut(s) | 324 |
| Hpy8I | GTNNAC | 7 cut(s) | 70, 82, 95, 115, 407, 491, 543 |
| Hpy99I | CGWCG | 1 cut(s) | 335 |
| HpyAV | CCTTC | 1 cut(s) | 337 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 191, 578 |
| HpyCH4IV | ACGT | 2 cut(s) | 84, 647 |
| HpyCH4V | TGCA | 4 cut(s) | 95, 167, 402, 451 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 56, 173, 192, 201, 353, 368, 379, 457, 563 |
| HpySE526I | ACGT | 2 cut(s) | 84, 647 |
| Hsp92II | CATG | 2 cut(s) | 127, 631 |
| HspAI | GCGC | 4 cut(s) | 265, 277, 347, 371 |
| KpnI | GGTACC | 2 cut(s) | 431, 458 |
| KspAI | GTTAAC | 3 cut(s) | 70, 82, 115 |
| Kzo9I | GATC | 1 cut(s) | 208 |
| LmnI | GCTCC | 2 cut(s) | 262, 606 |
| LweI | GCATC | 2 cut(s) | 389, 438 |
| MaeI | CTAG | 3 cut(s) | 120, 479, 567 |
| MaeII | ACGT | 2 cut(s) | 84, 647 |
| MaeIII | GTNAC | 2 cut(s) | 531, 614 |
| MalI | GATC | 1 cut(s) | 210 |
| MbiI | CCGCTC | 1 cut(s) | 552 |
| MboI | GATC | 1 cut(s) | 208 |
| MboII | GAAGA | 3 cut(s) | 41, 352, 432 |
| MfeI | CAATTG | 1 cut(s) | 536 |
| MflI | RGATCY | 1 cut(s) | 208 |
| MhlI | GDGCHC | 2 cut(s) | 97, 638 |
| MlsI | TGGCCA | 1 cut(s) | 311 |
| MluCI | AATT | 1 cut(s) | 536 |
| MluNI | TGGCCA | 1 cut(s) | 311 |
| MlyI | GAGTC | 1 cut(s) | 539 |
| MnlI | CCTC | 7 cut(s) | 166, 254, 286, 346, 504, 624, 634 |
| Mox20I | TGGCCA | 1 cut(s) | 311 |
| MscI | TGGCCA | 1 cut(s) | 311 |
| MseI | TTAA | 3 cut(s) | 69, 81, 114 |
| MslI | CAYNNNNRTG | 1 cut(s) | 446 |
| Msp20I | TGGCCA | 1 cut(s) | 311 |
| MspA1I | CMGCKG | 1 cut(s) | 460 |
| MspI | CCGG | 1 cut(s) | 417 |
| MspR9I | CCNGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| MunI | CAATTG | 1 cut(s) | 536 |
| MvaI | CCWGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| MvnI | CGCG | 2 cut(s) | 59, 267 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 56, 173, 192, 201, 353, 368, 379, 457, 563 |
| NdeII | GATC | 1 cut(s) | 208 |
| NlaIII | CATG | 2 cut(s) | 127, 631 |
| NlaIV | GGNNCC | 6 cut(s) | 129, 429, 456, 489, 635, 636 |
| NmeAIII | GCCGAG | 1 cut(s) | 533 |
| NmuCI | GTSAC | 1 cut(s) | 531 |
| PaqCI | CACCTGC | 1 cut(s) | 600 |
| PfeI | GAWTC | 1 cut(s) | 327 |
| PkrI | GCNGC | 1 cut(s) | 217 |
| PleI | GAGTC | 1 cut(s) | 538 |
| PpsI | GAGTC | 1 cut(s) | 538 |
| Ppu21I | YACGTR | 1 cut(s) | 648 |
| Psp1406I | AACGTT | 1 cut(s) | 84 |
| Psp6I | CCWGG | 5 cut(s) | 132, 181, 199, 204, 598 |
| PspFI | CCCAGC | 1 cut(s) | 367 |
| PspGI | CCWGG | 5 cut(s) | 132, 181, 199, 204, 598 |
| PspN4I | GGNNCC | 6 cut(s) | 129, 429, 456, 489, 635, 636 |
| PspOMI | GGGCCC | 1 cut(s) | 634 |
| PspPI | GGNCC | 4 cut(s) | 241, 488, 634, 635 |
| PstI | CTGCAG | 1 cut(s) | 169 |
| PstNI | CAGNNNCTG | 1 cut(s) | 151 |
| PsuI | RGATCY | 1 cut(s) | 208 |
| RsaI | GTAC | 4 cut(s) | 394, 429, 456, 542 |
| RsaNI | GTAC | 4 cut(s) | 393, 428, 455, 541 |
| RseI | CAYNNNNRTG | 1 cut(s) | 446 |
| SalI | GTCGAC | 1 cut(s) | 405 |
| SaqAI | TTAA | 3 cut(s) | 69, 81, 114 |
| SatI | GCNGC | 1 cut(s) | 216 |
| Sau3AI | GATC | 1 cut(s) | 208 |
| Sau96I | GGNCC | 4 cut(s) | 241, 488, 634, 635 |
| SchI | GAGTC | 1 cut(s) | 539 |
| ScrFI | CCNGG | 5 cut(s) | 134, 183, 201, 206, 600 |
| SduI | GDGCHC | 2 cut(s) | 97, 638 |
| SfaNI | GCATC | 2 cut(s) | 389, 438 |
| SfcI | CTRYAG | 1 cut(s) | 165 |
| SinI | GGWCC | 2 cut(s) | 241, 488 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 446 |
| SnaBI | TACGTA | 1 cut(s) | 648 |
| Sse9I | AATT | 1 cut(s) | 536 |
| SsiI | CCGC | 8 cut(s) | 48, 57, 216, 239, 432, 458, 527, 550 |
| SspMI | CTAG | 3 cut(s) | 120, 479, 567 |
| StyD4I | CCNGG | 5 cut(s) | 132, 181, 199, 204, 598 |
| TaaI | ACNGT | 3 cut(s) | 11, 191, 578 |
| TaiI | ACGT | 2 cut(s) | 87, 650 |
| TaqI | TCGA | 4 cut(s) | 178, 291, 330, 406 |
| TasI | AATT | 1 cut(s) | 536 |
| TatI | WGTACW | 1 cut(s) | 540 |
| TauI | GCSGC | 1 cut(s) | 218 |
| TfiI | GAWTC | 1 cut(s) | 327 |
| Tru1I | TTAA | 3 cut(s) | 69, 81, 114 |
| Tru9I | TTAA | 3 cut(s) | 69, 81, 114 |
| TscAI | CASTG | 2 cut(s) | 174, 645 |
| TseFI | GTSAC | 1 cut(s) | 531 |
| Tsp45I | GTSAC | 1 cut(s) | 531 |
| TspRI | CASTG | 2 cut(s) | 174, 645 |
| VneI | GTGCAC | 1 cut(s) | 93 |
| VpaK11BI | GGWCC | 2 cut(s) | 241, 488 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 248, 628 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 606 |
| XmiI | GTMKAC | 1 cut(s) | 406 |
| XspI | CTAG | 3 cut(s) | 120, 479, 567 |