Belongs to the RNase T2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 701 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 147 |
| AccBSI | CCGCTC | 1 cut(s) | 630 |
| AccII | CGCG | 1 cut(s) | 118 |
| AclWI | GGATC | 5 cut(s) | 285, 757, 770, 956, 969 |
| AcoI | YGGCCR | 3 cut(s) | 60, 561, 1019 |
| AcuI | CTGAAG | 1 cut(s) | 285 |
| AcyI | GRCGYC | 1 cut(s) | 888 |
| AfaI | GTAC | 1 cut(s) | 24 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 38, 147, 215, 376, 538, 662, 1062 |
| AgsI | TTSAA | 3 cut(s) | 355, 856, 1053 |
| AhlI | ACTAGT | 1 cut(s) | 451 |
| AjnI | CCWGG | 7 cut(s) | 384, 396, 456, 661, 742, 758, 957 |
| AluBI | AGCT | 7 cut(s) | 29, 138, 147, 481, 601, 614, 939 |
| AluI | AGCT | 7 cut(s) | 29, 138, 147, 481, 601, 614, 939 |
| Alw21I | GWGCWC | 1 cut(s) | 349 |
| Alw26I | GTCTC | 1 cut(s) | 419 |
| AlwI | GGATC | 5 cut(s) | 285, 757, 770, 956, 969 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 147 |
| AoxI | GGCC | 8 cut(s) | 60, 217, 380, 546, 561, 633, 771, 1019 |
| ApaI | GGGCCC | 2 cut(s) | 384, 637 |
| ApeKI | GCWGC | 1 cut(s) | 438 |
| AspLEI | GCGC | 2 cut(s) | 120, 712 |
| AspS9I | GGNCC | 9 cut(s) | 380, 381, 400, 460, 493, 547, 633, 634, 746 |
| AsuC2I | CCSGG | 1 cut(s) | 86 |
| AsuHPI | GGTGA | 4 cut(s) | 94, 198, 217, 226 |
| AvaII | GGWCC | 4 cut(s) | 400, 460, 493, 746 |
| BaeGI | GKGCMC | 3 cut(s) | 384, 637, 706 |
| BalI | TGGCCA | 1 cut(s) | 563 |
| BamHI | GGATCC | 2 cut(s) | 762, 961 |
| BanI | GGYRCC | 1 cut(s) | 701 |
| BanII | GRGCYC | 3 cut(s) | 384, 392, 637 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 181, 213 |
| Bbv12I | GWGCWC | 1 cut(s) | 349 |
| BbvI | GCAGC | 1 cut(s) | 450 |
| BccI | CCATC | 2 cut(s) | 228, 303 |
| BceAI | ACGGC | 4 cut(s) | 75, 750, 758, 835 |
| BciT130I | CCWGG | 7 cut(s) | 386, 398, 458, 663, 744, 760, 959 |
| BcnI | CCSGG | 1 cut(s) | 86 |
| BcoDI | GTCTC | 1 cut(s) | 419 |
| BcuI | ACTAGT | 1 cut(s) | 451 |
| BfaI | CTAG | 4 cut(s) | 452, 681, 698, 738 |
| BfmI | CTRYAG | 1 cut(s) | 767 |
| BfoI | RGCGCY | 1 cut(s) | 713 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 68 |
| BisI | GCNGC | 5 cut(s) | 312, 439, 468, 955, 1022 |
| BlsI | GCNGC | 5 cut(s) | 313, 440, 469, 956, 1023 |
| Bme1390I | CCNGG | 8 cut(s) | 86, 386, 398, 458, 663, 744, 760, 959 |
| Bme18I | GGWCC | 4 cut(s) | 400, 460, 493, 746 |
| BmgT120I | GGNCC | 9 cut(s) | 380, 381, 400, 460, 493, 547, 633, 634, 746 |
| BmiI | GGNNCC | 9 cut(s) | 381, 382, 383, 402, 634, 635, 703, 764, 963 |
| BmrFI | CCNGG | 8 cut(s) | 86, 386, 398, 458, 663, 744, 760, 959 |
| BmsI | GCATC | 1 cut(s) | 138 |
| BpmI | CTGGAG | 1 cut(s) | 124 |
| Bpu10I | CCTNAGC | 2 cut(s) | 79, 371 |
| BpuEI | CTTGAG | 1 cut(s) | 1042 |
| BpuMI | CCSGG | 1 cut(s) | 86 |
| BsaHI | GRCGYC | 1 cut(s) | 888 |
| BsaI | GGTCTC | 1 cut(s) | 419 |
| BsaJI | CCNNGG | 4 cut(s) | 84, 384, 385, 396 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 38, 147, 215, 376, 538, 662, 1062 |
| Bse3DI | GCAATG | 1 cut(s) | 939 |
| BseBI | CCWGG | 7 cut(s) | 386, 398, 458, 663, 744, 760, 959 |
| BseDI | CCNNGG | 4 cut(s) | 84, 384, 385, 396 |
| BseGI | GGATG | 4 cut(s) | 295, 392, 837, 1010 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 38, 147, 215, 376, 538, 662, 1062 |
| BseMI | GCAATG | 1 cut(s) | 939 |
| BseMII | CTCAG | 1 cut(s) | 1057 |
| BseSI | GKGCMC | 3 cut(s) | 384, 637, 706 |
| BseXI | GCAGC | 1 cut(s) | 450 |
| BseYI | CCCAGC | 6 cut(s) | 16, 147, 307, 325, 619, 706 |
| Bsh1236I | CGCG | 1 cut(s) | 118 |
| BshFI | GGCC | 8 cut(s) | 62, 219, 382, 548, 563, 635, 773, 1021 |
| BshNI | GGYRCC | 1 cut(s) | 701 |
| BsiHKAI | GWGCWC | 1 cut(s) | 349 |
| BsiSI | CCGG | 1 cut(s) | 86 |
| BslFI | GGGAC | 2 cut(s) | 153, 386 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 38, 147, 215, 376, 538, 662, 1062 |
| BsmAI | GTCTC | 1 cut(s) | 419 |
| BsmFI | GGGAC | 2 cut(s) | 153, 386 |
| BsmI | GAATGC | 2 cut(s) | 533, 676 |
| BsnI | GGCC | 8 cut(s) | 62, 219, 382, 548, 563, 635, 773, 1021 |
| Bso31I | GGTCTC | 1 cut(s) | 419 |
| Bsp120I | GGGCCC | 2 cut(s) | 380, 633 |
| Bsp1286I | GDGCHC | 5 cut(s) | 349, 384, 392, 637, 706 |
| Bsp143I | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| BspANI | GGCC | 8 cut(s) | 62, 219, 382, 548, 563, 635, 773, 1021 |
| BspCNI | CTCAG | 1 cut(s) | 1056 |
| BspFNI | CGCG | 1 cut(s) | 118 |
| BspLI | GGNNCC | 9 cut(s) | 381, 382, 383, 402, 634, 635, 703, 764, 963 |
| BspMAI | CTGCAG | 1 cut(s) | 771 |
| BspPI | GGATC | 5 cut(s) | 285, 757, 770, 956, 969 |
| BspQI | GCTCTTC | 1 cut(s) | 261 |
| BspT107I | GGYRCC | 1 cut(s) | 701 |
| BspTNI | GGTCTC | 1 cut(s) | 419 |
| BsrBI | CCGCTC | 1 cut(s) | 630 |
| BsrDI | GCAATG | 1 cut(s) | 939 |
| BssECI | CCNNGG | 4 cut(s) | 84, 384, 385, 396 |
| BssMI | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| BssNI | GRCGYC | 1 cut(s) | 888 |
| Bst2UI | CCWGG | 7 cut(s) | 386, 398, 458, 663, 744, 760, 959 |
| Bst4CI | ACNGT | 3 cut(s) | 248, 344, 926 |
| Bst6I | CTCTTC | 1 cut(s) | 261 |
| BstACI | GRCGYC | 1 cut(s) | 888 |
| BstC8I | GCNNGC | 5 cut(s) | 69, 273, 637, 641, 771 |
| BstDEI | CTNAG | 3 cut(s) | 79, 371, 1043 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 213, 1060 |
| BstF5I | GGATG | 4 cut(s) | 295, 392, 837, 1010 |
| BstFNI | CGCG | 1 cut(s) | 118 |
| BstH2I | RGCGCY | 1 cut(s) | 713 |
| BstHHI | GCGC | 2 cut(s) | 120, 712 |
| BstKTI | GATC | 5 cut(s) | 102, 194, 280, 765, 964 |
| BstMAI | GTCTC | 1 cut(s) | 419 |
| BstMBI | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| BstNI | CCWGG | 7 cut(s) | 386, 398, 458, 663, 744, 760, 959 |
| BstNSI | RCATGY | 1 cut(s) | 791 |
| BstSCI | CCNGG | 8 cut(s) | 84, 384, 396, 456, 661, 742, 758, 957 |
| BstSFI | CTRYAG | 1 cut(s) | 767 |
| BstSLI | GKGCMC | 3 cut(s) | 384, 637, 706 |
| BstUI | CGCG | 1 cut(s) | 118 |
| BstV1I | GCAGC | 1 cut(s) | 450 |
| BstX2I | RGATCY | 2 cut(s) | 762, 961 |
| BstYI | RGATCY | 2 cut(s) | 762, 961 |
| BsuRI | GGCC | 8 cut(s) | 62, 219, 382, 548, 563, 635, 773, 1021 |
| BtsCI | GGATG | 4 cut(s) | 295, 392, 837, 1010 |
| BtsI | GCAGTG | 1 cut(s) | 753 |
| BtsIMutI | CAGTG | 3 cut(s) | 404, 753, 931 |
| Cac8I | GCNNGC | 5 cut(s) | 69, 273, 637, 641, 771 |
| CaiI | CAGNNNCTG | 1 cut(s) | 147 |
| CfoI | GCGC | 2 cut(s) | 120, 712 |
| Cfr13I | GGNCC | 9 cut(s) | 380, 381, 400, 460, 493, 547, 633, 634, 746 |
| CseI | GACGC | 2 cut(s) | 119, 896 |
| Csp6I | GTAC | 1 cut(s) | 23 |
| CviAII | CATG | 7 cut(s) | 7, 183, 238, 788, 823, 844, 979 |
| CviQI | GTAC | 1 cut(s) | 23 |
| DdeI | CTNAG | 3 cut(s) | 79, 371, 1043 |
| DpnI | GATC | 5 cut(s) | 101, 193, 279, 764, 963 |
| DpnII | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| EaeI | YGGCCR | 3 cut(s) | 60, 561, 1019 |
| Eam1104I | CTCTTC | 1 cut(s) | 261 |
| EarI | CTCTTC | 1 cut(s) | 261 |
| EciI | GGCGGA | 1 cut(s) | 557 |
| Eco24I | GRGCYC | 3 cut(s) | 384, 392, 637 |
| Eco31I | GGTCTC | 1 cut(s) | 419 |
| Eco47I | GGWCC | 4 cut(s) | 400, 460, 493, 746 |
| Eco57I | CTGAAG | 1 cut(s) | 285 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 213, 1060 |
| EcoO109I | RGGNCCY | 5 cut(s) | 380, 381, 400, 460, 633 |
| EcoRII | CCWGG | 7 cut(s) | 384, 396, 456, 661, 742, 758, 957 |
| EcoT38I | GRGCYC | 3 cut(s) | 384, 392, 637 |
| FaeI | CATG | 7 cut(s) | 10, 186, 241, 791, 826, 847, 982 |
| FaqI | GGGAC | 2 cut(s) | 153, 386 |
| FatI | CATG | 7 cut(s) | 6, 182, 237, 787, 822, 843, 978 |
| FauI | CCCGC | 5 cut(s) | 40, 60, 245, 623, 644 |
| Fnu4HI | GCNGC | 5 cut(s) | 312, 439, 468, 955, 1022 |
| FokI | GGATG | 4 cut(s) | 282, 379, 824, 997 |
| FriOI | GRGCYC | 3 cut(s) | 384, 392, 637 |
| Fsp4HI | GCNGC | 5 cut(s) | 312, 439, 468, 955, 1022 |
| FspBI | CTAG | 4 cut(s) | 452, 681, 698, 738 |
| GlaI | GCGC | 2 cut(s) | 119, 711 |
| GluI | GCNGC | 5 cut(s) | 312, 439, 468, 955, 1022 |
| GsaI | CCCAGC | 6 cut(s) | 20, 151, 311, 329, 623, 710 |
| GsuI | CTGGAG | 1 cut(s) | 124 |
| HaeII | RGCGCY | 1 cut(s) | 713 |
| HaeIII | GGCC | 8 cut(s) | 62, 219, 382, 548, 563, 635, 773, 1021 |
| HapII | CCGG | 1 cut(s) | 86 |
| HgaI | GACGC | 2 cut(s) | 119, 896 |
| HhaI | GCGC | 2 cut(s) | 120, 712 |
| Hin1I | GRCGYC | 1 cut(s) | 888 |
| Hin1II | CATG | 7 cut(s) | 10, 186, 241, 791, 826, 847, 982 |
| Hin6I | GCGC | 2 cut(s) | 118, 710 |
| HinP1I | GCGC | 2 cut(s) | 118, 710 |
| HincII | GTYRAC | 1 cut(s) | 340 |
| HindII | GTYRAC | 1 cut(s) | 340 |
| HinfI | GANTC | 1 cut(s) | 970 |
| HpaI | GTTAAC | 1 cut(s) | 340 |
| HpaII | CCGG | 1 cut(s) | 86 |
| HphI | GGTGA | 4 cut(s) | 94, 198, 217, 226 |
| Hpy166II | GTNNAC | 1 cut(s) | 340 |
| Hpy188I | TCNGA | 2 cut(s) | 134, 779 |
| Hpy188III | TCNNGA | 1 cut(s) | 141 |
| Hpy8I | GTNNAC | 1 cut(s) | 340 |
| Hpy99I | CGWCG | 1 cut(s) | 587 |
| HpyAV | CCTTC | 3 cut(s) | 532, 844, 862 |
| HpyCH4III | ACNGT | 3 cut(s) | 248, 344, 926 |
| HpyCH4V | TGCA | 4 cut(s) | 648, 674, 769, 791 |
| HpyF3I | CTNAG | 3 cut(s) | 79, 371, 1043 |
| Hsp92I | GRCGYC | 1 cut(s) | 888 |
| Hsp92II | CATG | 7 cut(s) | 10, 186, 241, 791, 826, 847, 982 |
| HspAI | GCGC | 2 cut(s) | 118, 710 |
| KspAI | GTTAAC | 1 cut(s) | 340 |
| Kzo9I | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| LguI | GCTCTTC | 1 cut(s) | 261 |
| LmnI | GCTCC | 2 cut(s) | 34, 143 |
| Lsp1109I | GCAGC | 1 cut(s) | 450 |
| LweI | GCATC | 1 cut(s) | 138 |
| MaeI | CTAG | 4 cut(s) | 452, 681, 698, 738 |
| MaeIII | GTNAC | 4 cut(s) | 9, 43, 807, 862 |
| MalI | GATC | 5 cut(s) | 101, 193, 279, 764, 963 |
| MbiI | CCGCTC | 1 cut(s) | 630 |
| MboI | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| MboII | GAAGA | 3 cut(s) | 201, 278, 814 |
| MflI | RGATCY | 2 cut(s) | 762, 961 |
| MhlI | GDGCHC | 5 cut(s) | 349, 384, 392, 637, 706 |
| MlsI | TGGCCA | 1 cut(s) | 563 |
| MluNI | TGGCCA | 1 cut(s) | 563 |
| MlyI | GAGTC | 1 cut(s) | 964 |
| MnlI | CCTC | 9 cut(s) | 473, 506, 508, 538, 735, 905, 922, 1065, 1066 |
| Mox20I | TGGCCA | 1 cut(s) | 563 |
| MscI | TGGCCA | 1 cut(s) | 563 |
| MseI | TTAA | 4 cut(s) | 321, 333, 339, 1081 |
| MslI | CAYNNNNRTG | 2 cut(s) | 569, 842 |
| Msp20I | TGGCCA | 1 cut(s) | 563 |
| MspA1I | CMGCKG | 4 cut(s) | 107, 311, 329, 639 |
| MspI | CCGG | 1 cut(s) | 86 |
| MspR9I | CCNGG | 8 cut(s) | 86, 386, 398, 458, 663, 744, 760, 959 |
| Mva1269I | GAATGC | 2 cut(s) | 533, 676 |
| MvaI | CCWGG | 7 cut(s) | 386, 398, 458, 663, 744, 760, 959 |
| MvnI | CGCG | 1 cut(s) | 118 |
| NciI | CCSGG | 1 cut(s) | 86 |
| NdeII | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| NlaIII | CATG | 7 cut(s) | 10, 186, 241, 791, 826, 847, 982 |
| NlaIV | GGNNCC | 9 cut(s) | 381, 382, 383, 402, 634, 635, 703, 764, 963 |
| NmeAIII | GCCGAG | 1 cut(s) | 557 |
| NmuCI | GTSAC | 3 cut(s) | 43, 807, 862 |
| NspI | RCATGY | 1 cut(s) | 791 |
| PasI | CCCWGGG | 1 cut(s) | 385 |
| PciSI | GCTCTTC | 1 cut(s) | 261 |
| PctI | GAATGC | 2 cut(s) | 533, 676 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 147 |
| PfoI | TCCNGGA | 2 cut(s) | 456, 661 |
| PkrI | GCNGC | 5 cut(s) | 313, 440, 469, 956, 1023 |
| PleI | GAGTC | 1 cut(s) | 964 |
| PpsI | GAGTC | 1 cut(s) | 964 |
| PpuMI | RGGWCCY | 2 cut(s) | 400, 460 |
| Psp5II | RGGWCCY | 2 cut(s) | 400, 460 |
| Psp6I | CCWGG | 7 cut(s) | 384, 396, 456, 661, 742, 758, 957 |
| PspFI | CCCAGC | 6 cut(s) | 16, 147, 307, 325, 619, 706 |
| PspGI | CCWGG | 7 cut(s) | 384, 396, 456, 661, 742, 758, 957 |
| PspN4I | GGNNCC | 9 cut(s) | 381, 382, 383, 402, 634, 635, 703, 764, 963 |
| PspOMI | GGGCCC | 2 cut(s) | 380, 633 |
| PspPI | GGNCC | 9 cut(s) | 380, 381, 400, 460, 493, 547, 633, 634, 746 |
| PspPPI | RGGWCCY | 2 cut(s) | 400, 460 |
| PstI | CTGCAG | 1 cut(s) | 771 |
| PstNI | CAGNNNCTG | 1 cut(s) | 147 |
| PsuI | RGATCY | 2 cut(s) | 762, 961 |
| RsaI | GTAC | 1 cut(s) | 24 |
| RsaNI | GTAC | 1 cut(s) | 23 |
| RseI | CAYNNNNRTG | 2 cut(s) | 569, 842 |
| SapI | GCTCTTC | 1 cut(s) | 261 |
| SaqAI | TTAA | 4 cut(s) | 321, 333, 339, 1081 |
| SatI | GCNGC | 5 cut(s) | 312, 439, 468, 955, 1022 |
| Sau3AI | GATC | 5 cut(s) | 99, 191, 277, 762, 961 |
| Sau96I | GGNCC | 9 cut(s) | 380, 381, 400, 460, 493, 547, 633, 634, 746 |
| SbfI | CCTGCAGG | 1 cut(s) | 771 |
| SchI | GAGTC | 1 cut(s) | 964 |
| ScrFI | CCNGG | 8 cut(s) | 86, 386, 398, 458, 663, 744, 760, 959 |
| SdaI | CCTGCAGG | 1 cut(s) | 771 |
| SduI | GDGCHC | 5 cut(s) | 349, 384, 392, 637, 706 |
| SfaNI | GCATC | 1 cut(s) | 138 |
| SfcI | CTRYAG | 1 cut(s) | 767 |
| SinI | GGWCC | 4 cut(s) | 400, 460, 493, 746 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 569, 842 |
| SmlI | CTYRAG | 1 cut(s) | 1057 |
| SmoI | CTYRAG | 1 cut(s) | 1057 |
| SpeI | ACTAGT | 1 cut(s) | 451 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 771 |
| SspI | AATATT | 1 cut(s) | 1079 |
| SspMI | CTAG | 4 cut(s) | 452, 681, 698, 738 |
| StyD4I | CCNGG | 8 cut(s) | 84, 384, 396, 456, 661, 742, 758, 957 |
| TaaI | ACNGT | 3 cut(s) | 248, 344, 926 |
| TaqI | TCGA | 2 cut(s) | 176, 582 |
| TauI | GCSGC | 4 cut(s) | 314, 470, 957, 1024 |
| Tru1I | TTAA | 4 cut(s) | 321, 333, 339, 1081 |
| Tru9I | TTAA | 4 cut(s) | 321, 333, 339, 1081 |
| TscAI | CASTG | 3 cut(s) | 411, 760, 931 |
| TseFI | GTSAC | 3 cut(s) | 43, 807, 862 |
| TseI | GCWGC | 1 cut(s) | 438 |
| Tsp45I | GTSAC | 3 cut(s) | 43, 807, 862 |
| TspDTI | ATGAA | 4 cut(s) | 826, 839, 967, 999 |
| TspGWI | ACGGA | 1 cut(s) | 437 |
| TspRI | CASTG | 3 cut(s) | 411, 760, 931 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 147 |
| VpaK11BI | GGWCC | 4 cut(s) | 400, 460, 493, 746 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 213, 1060 |
| XceI | RCATGY | 1 cut(s) | 791 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 373 |
| XspI | CTAG | 4 cut(s) | 452, 681, 698, 738 |