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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 151 |
| Acc36I | ACCTGC | 2 cut(s) | 151, 298 |
| Acc65I | GGTACC | 2 cut(s) | 283, 310 |
| AccB1I | GGYRCC | 2 cut(s) | 283, 310 |
| AccBSI | CCGCTC | 2 cut(s) | 51, 408 |
| AccII | CGCG | 2 cut(s) | 123, 244 |
| AciI | CCGC | 9 cut(s) | 13, 46, 49, 72, 95, 244, 276, 406, 469 |
| AclI | AACGTT | 1 cut(s) | 20 |
| AcoI | YGGCCR | 1 cut(s) | 165 |
| AcuI | CTGAAG | 1 cut(s) | 375 |
| AfaI | GTAC | 2 cut(s) | 285, 312 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 55, 61, 106, 272, 450 |
| AgsI | TTSAA | 1 cut(s) | 39 |
| AjnI | CCWGG | 4 cut(s) | 251, 416, 449, 477 |
| AluBI | AGCT | 3 cut(s) | 85, 331, 460 |
| AluI | AGCT | 3 cut(s) | 85, 331, 460 |
| Alw21I | GWGCWC | 1 cut(s) | 251 |
| AoxI | GGCC | 3 cut(s) | 144, 165, 420 |
| Asp718I | GGTACC | 2 cut(s) | 283, 310 |
| AspLEI | GCGC | 4 cut(s) | 91, 123, 205, 258 |
| AspS9I | GGNCC | 5 cut(s) | 64, 97, 344, 420, 485 |
| AsuC2I | CCSGG | 1 cut(s) | 56 |
| AsuNHI | GCTAGC | 1 cut(s) | 91 |
| AvaII | GGWCC | 4 cut(s) | 64, 97, 344, 485 |
| BalI | TGGCCA | 1 cut(s) | 167 |
| BanI | GGYRCC | 2 cut(s) | 283, 310 |
| Bbv12I | GWGCWC | 1 cut(s) | 251 |
| BciT130I | CCWGG | 4 cut(s) | 253, 418, 451, 479 |
| BcnI | CCSGG | 1 cut(s) | 56 |
| BfaI | CTAG | 2 cut(s) | 92, 371 |
| BfoI | RGCGCY | 2 cut(s) | 92, 206 |
| BfuAI | ACCTGC | 2 cut(s) | 151, 298 |
| BisI | GCNGC | 4 cut(s) | 46, 49, 72, 406 |
| BlsI | GCNGC | 4 cut(s) | 47, 50, 73, 407 |
| Bme1390I | CCNGG | 5 cut(s) | 56, 253, 418, 451, 479 |
| Bme18I | GGWCC | 4 cut(s) | 64, 97, 344, 485 |
| BmgT120I | GGNCC | 5 cut(s) | 64, 97, 344, 420, 485 |
| BmiI | GGNNCC | 4 cut(s) | 285, 312, 345, 487 |
| BmrFI | CCNGG | 5 cut(s) | 56, 253, 418, 451, 479 |
| BmsI | GCATC | 1 cut(s) | 294 |
| BmtI | GCTAGC | 1 cut(s) | 95 |
| BpuMI | CCSGG | 1 cut(s) | 56 |
| BsaJI | CCNNGG | 5 cut(s) | 54, 147, 417, 429, 449 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 55, 61, 106, 272, 450 |
| Bse118I | RCCGGY | 1 cut(s) | 272 |
| BseBI | CCWGG | 4 cut(s) | 253, 418, 451, 479 |
| BseDI | CCNNGG | 5 cut(s) | 54, 147, 417, 429, 449 |
| BseGI | GGATG | 1 cut(s) | 474 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 55, 61, 106, 272, 450 |
| BsgI | GTGCAG | 1 cut(s) | 80 |
| Bsh1236I | CGCG | 2 cut(s) | 123, 244 |
| BshFI | GGCC | 3 cut(s) | 146, 167, 422 |
| BshNI | GGYRCC | 2 cut(s) | 283, 310 |
| BsiHKAI | GWGCWC | 1 cut(s) | 251 |
| BsiSI | CCGG | 2 cut(s) | 56, 273 |
| BslFI | GGGAC | 2 cut(s) | 357, 431 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 55, 61, 106, 272, 450 |
| BsmFI | GGGAC | 2 cut(s) | 357, 431 |
| BsnI | GGCC | 3 cut(s) | 146, 167, 422 |
| Bsp1286I | GDGCHC | 1 cut(s) | 251 |
| BspACI | CCGC | 9 cut(s) | 13, 46, 49, 72, 95, 244, 276, 406, 469 |
| BspANI | GGCC | 3 cut(s) | 146, 167, 422 |
| BspFNI | CGCG | 2 cut(s) | 123, 244 |
| BspLI | GGNNCC | 4 cut(s) | 285, 312, 345, 487 |
| BspMI | ACCTGC | 2 cut(s) | 151, 298 |
| BspOI | GCTAGC | 1 cut(s) | 95 |
| BspT107I | GGYRCC | 2 cut(s) | 283, 310 |
| BsrBI | CCGCTC | 2 cut(s) | 51, 408 |
| BsrFI | RCCGGY | 1 cut(s) | 272 |
| BssAI | RCCGGY | 1 cut(s) | 272 |
| BssECI | CCNNGG | 5 cut(s) | 54, 147, 417, 429, 449 |
| Bst2UI | CCWGG | 4 cut(s) | 253, 418, 451, 479 |
| Bst4CI | ACNGT | 1 cut(s) | 28 |
| Bst6I | CTCTTC | 1 cut(s) | 191 |
| BstC8I | GCNNGC | 3 cut(s) | 87, 93, 236 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 104 |
| BstF5I | GGATG | 1 cut(s) | 474 |
| BstFNI | CGCG | 2 cut(s) | 123, 244 |
| BstH2I | RGCGCY | 2 cut(s) | 92, 206 |
| BstHHI | GCGC | 4 cut(s) | 91, 123, 205, 258 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 235, 255, 262, 466 |
| BstNI | CCWGG | 4 cut(s) | 253, 418, 451, 479 |
| BstSCI | CCNGG | 5 cut(s) | 54, 251, 416, 449, 477 |
| BstUI | CGCG | 2 cut(s) | 123, 244 |
| BsuRI | GGCC | 3 cut(s) | 146, 167, 422 |
| BtsCI | GGATG | 1 cut(s) | 474 |
| BveI | ACCTGC | 2 cut(s) | 151, 298 |
| Cac8I | GCNNGC | 3 cut(s) | 87, 93, 236 |
| CfoI | GCGC | 4 cut(s) | 91, 123, 205, 258 |
| Cfr10I | RCCGGY | 1 cut(s) | 272 |
| Cfr13I | GGNCC | 5 cut(s) | 64, 97, 344, 420, 485 |
| Csp6I | GTAC | 2 cut(s) | 284, 311 |
| CviQI | GTAC | 2 cut(s) | 284, 311 |
| EaeI | YGGCCR | 1 cut(s) | 165 |
| Eam1104I | CTCTTC | 1 cut(s) | 191 |
| EarI | CTCTTC | 1 cut(s) | 191 |
| EciI | GGCGGA | 1 cut(s) | 291 |
| Eco47I | GGWCC | 4 cut(s) | 64, 97, 344, 485 |
| Eco57I | CTGAAG | 1 cut(s) | 375 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 104 |
| EcoO109I | RGGNCCY | 2 cut(s) | 64, 485 |
| EcoRII | CCWGG | 4 cut(s) | 251, 416, 449, 477 |
| FaiI | YATR | 4 cut(s) | 81, 176, 299, 399 |
| FaqI | GGGAC | 2 cut(s) | 357, 431 |
| Fnu4HI | GCNGC | 4 cut(s) | 46, 49, 72, 406 |
| FokI | GGATG | 1 cut(s) | 461 |
| Fsp4HI | GCNGC | 4 cut(s) | 46, 49, 72, 406 |
| FspBI | CTAG | 2 cut(s) | 92, 371 |
| GlaI | GCGC | 4 cut(s) | 90, 122, 204, 257 |
| GluI | GCNGC | 4 cut(s) | 46, 49, 72, 406 |
| HaeII | RGCGCY | 2 cut(s) | 92, 206 |
| HaeIII | GGCC | 3 cut(s) | 146, 167, 422 |
| HapII | CCGG | 2 cut(s) | 56, 273 |
| HhaI | GCGC | 4 cut(s) | 91, 123, 205, 258 |
| Hin6I | GCGC | 4 cut(s) | 89, 121, 203, 256 |
| HinP1I | GCGC | 4 cut(s) | 89, 121, 203, 256 |
| HincII | GTYRAC | 2 cut(s) | 18, 24 |
| HindII | GTYRAC | 2 cut(s) | 18, 24 |
| HinfI | GANTC | 1 cut(s) | 386 |
| HpaI | GTTAAC | 2 cut(s) | 18, 24 |
| HpaII | CCGG | 2 cut(s) | 56, 273 |
| Hpy166II | GTNNAC | 2 cut(s) | 18, 24 |
| Hpy188III | TCNNGA | 3 cut(s) | 116, 180, 353 |
| Hpy8I | GTNNAC | 2 cut(s) | 18, 24 |
| HpyAV | CCTTC | 1 cut(s) | 110 |
| HpyCH4III | ACNGT | 1 cut(s) | 28 |
| HpyCH4IV | ACGT | 1 cut(s) | 20 |
| HpyCH4V | TGCA | 4 cut(s) | 61, 135, 229, 307 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 235, 255, 262, 466 |
| HpySE526I | ACGT | 1 cut(s) | 20 |
| HspAI | GCGC | 4 cut(s) | 89, 121, 203, 256 |
| KpnI | GGTACC | 2 cut(s) | 287, 314 |
| KspAI | GTTAAC | 2 cut(s) | 18, 24 |
| LmnI | GCTCC | 2 cut(s) | 56, 246 |
| LweI | GCATC | 1 cut(s) | 294 |
| MaeI | CTAG | 2 cut(s) | 92, 371 |
| MaeII | ACGT | 1 cut(s) | 20 |
| MaeIII | GTNAC | 1 cut(s) | 387 |
| MbiI | CCGCTC | 2 cut(s) | 51, 408 |
| MboII | GAAGA | 2 cut(s) | 208, 221 |
| MhlI | GDGCHC | 1 cut(s) | 251 |
| MlsI | TGGCCA | 1 cut(s) | 167 |
| MluNI | TGGCCA | 1 cut(s) | 167 |
| MlyI | GAGTC | 1 cut(s) | 395 |
| MnlI | CCTC | 5 cut(s) | 77, 142, 192, 373, 424 |
| Mox20I | TGGCCA | 1 cut(s) | 167 |
| MscI | TGGCCA | 1 cut(s) | 167 |
| MseI | TTAA | 2 cut(s) | 17, 23 |
| MslI | CAYNNNNRTG | 2 cut(s) | 173, 302 |
| Msp20I | TGGCCA | 1 cut(s) | 167 |
| MspA1I | CMGCKG | 2 cut(s) | 13, 469 |
| MspI | CCGG | 2 cut(s) | 56, 273 |
| MspR9I | CCNGG | 5 cut(s) | 56, 253, 418, 451, 479 |
| MvaI | CCWGG | 4 cut(s) | 253, 418, 451, 479 |
| MvnI | CGCG | 2 cut(s) | 123, 244 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 235, 255, 262, 466 |
| NciI | CCSGG | 1 cut(s) | 56 |
| NheI | GCTAGC | 1 cut(s) | 91 |
| NlaIV | GGNNCC | 4 cut(s) | 285, 312, 345, 487 |
| NmeAIII | GCCGAG | 1 cut(s) | 172 |
| NmuCI | GTSAC | 1 cut(s) | 387 |
| PaqCI | CACCTGC | 1 cut(s) | 151 |
| PkrI | GCNGC | 4 cut(s) | 47, 50, 73, 407 |
| PleI | GAGTC | 1 cut(s) | 394 |
| PpsI | GAGTC | 1 cut(s) | 394 |
| PpuMI | RGGWCCY | 2 cut(s) | 64, 485 |
| Psp1406I | AACGTT | 1 cut(s) | 20 |
| Psp5II | RGGWCCY | 2 cut(s) | 64, 485 |
| Psp6I | CCWGG | 4 cut(s) | 251, 416, 449, 477 |
| PspGI | CCWGG | 4 cut(s) | 251, 416, 449, 477 |
| PspN4I | GGNNCC | 4 cut(s) | 285, 312, 345, 487 |
| PspPI | GGNCC | 5 cut(s) | 64, 97, 344, 420, 485 |
| PspPPI | RGGWCCY | 2 cut(s) | 64, 485 |
| RsaI | GTAC | 2 cut(s) | 285, 312 |
| RsaNI | GTAC | 2 cut(s) | 284, 311 |
| RseI | CAYNNNNRTG | 2 cut(s) | 173, 302 |
| SaqAI | TTAA | 2 cut(s) | 17, 23 |
| SatI | GCNGC | 4 cut(s) | 46, 49, 72, 406 |
| Sau96I | GGNCC | 5 cut(s) | 64, 97, 344, 420, 485 |
| SchI | GAGTC | 1 cut(s) | 395 |
| ScrFI | CCNGG | 5 cut(s) | 56, 253, 418, 451, 479 |
| SduI | GDGCHC | 1 cut(s) | 251 |
| SetI | ASST | 8 cut(s) | 23, 69, 87, 102, 165, 312, 333, 462 |
| SfaNI | GCATC | 1 cut(s) | 294 |
| SgrAI | CRCCGGYG | 1 cut(s) | 272 |
| SinI | GGWCC | 4 cut(s) | 64, 97, 344, 485 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 173, 302 |
| SsiI | CCGC | 9 cut(s) | 13, 46, 49, 72, 95, 244, 276, 406, 469 |
| SspMI | CTAG | 2 cut(s) | 92, 371 |
| StyD4I | CCNGG | 5 cut(s) | 54, 251, 416, 449, 477 |
| TaaI | ACNGT | 1 cut(s) | 28 |
| TaiI | ACGT | 1 cut(s) | 23 |
| TaqI | TCGA | 1 cut(s) | 115 |
| TauI | GCSGC | 4 cut(s) | 48, 51, 74, 408 |
| Tru1I | TTAA | 2 cut(s) | 17, 23 |
| Tru9I | TTAA | 2 cut(s) | 17, 23 |
| TseFI | GTSAC | 1 cut(s) | 387 |
| Tsp45I | GTSAC | 1 cut(s) | 387 |
| TspDTI | ATGAA | 1 cut(s) | 204 |
| VpaK11BI | GGWCC | 4 cut(s) | 64, 97, 344, 485 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 104 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 457 |
| XspI | CTAG | 2 cut(s) | 92, 371 |