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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 422 |
| AccB1I | GGYRCC | 1 cut(s) | 245 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 476 |
| AccBSI | CCGCTC | 1 cut(s) | 370 |
| AccI | GTMKAC | 1 cut(s) | 224 |
| AccII | CGCG | 1 cut(s) | 132 |
| AclWI | GGATC | 4 cut(s) | 34, 67, 393, 503 |
| AcoI | YGGCCR | 2 cut(s) | 127, 172 |
| AcyI | GRCGYC | 1 cut(s) | 246 |
| AfaI | GTAC | 3 cut(s) | 42, 262, 274 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 122, 234, 385, 412, 476 |
| AflIII | ACRYGT | 1 cut(s) | 141 |
| AjnI | CCWGG | 5 cut(s) | 22, 213, 248, 287, 378 |
| AluBI | AGCT | 1 cut(s) | 473 |
| AluI | AGCT | 1 cut(s) | 473 |
| Alw26I | GTCTC | 2 cut(s) | 113, 513 |
| AlwI | GGATC | 4 cut(s) | 34, 67, 393, 503 |
| AoxI | GGCC | 7 cut(s) | 20, 106, 127, 172, 252, 442, 569 |
| ApeKI | GCWGC | 3 cut(s) | 4, 7, 208 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 352 |
| AspA2I | CCTAGG | 1 cut(s) | 411 |
| AspLEI | GCGC | 4 cut(s) | 97, 134, 234, 248 |
| AspS9I | GGNCC | 3 cut(s) | 252, 306, 570 |
| AsuC2I | CCSGG | 4 cut(s) | 186, 513, 542, 568 |
| AsuHPI | GGTGA | 1 cut(s) | 26 |
| AvaII | GGWCC | 1 cut(s) | 306 |
| AvrII | CCTAGG | 1 cut(s) | 411 |
| BanI | GGYRCC | 1 cut(s) | 245 |
| BbvI | GCAGC | 3 cut(s) | 16, 19, 220 |
| BccI | CCATC | 2 cut(s) | 41, 335 |
| BciT130I | CCWGG | 5 cut(s) | 24, 215, 250, 289, 380 |
| BcnI | CCSGG | 4 cut(s) | 186, 513, 542, 568 |
| BcoDI | GTCTC | 2 cut(s) | 113, 513 |
| BfaI | CTAG | 1 cut(s) | 412 |
| BfoI | RGCGCY | 2 cut(s) | 235, 249 |
| BfuAI | ACCTGC | 1 cut(s) | 422 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 19, 428 |
| BisI | GCNGC | 7 cut(s) | 5, 8, 11, 130, 172, 209, 312 |
| BlnI | CCTAGG | 1 cut(s) | 411 |
| BlsI | GCNGC | 7 cut(s) | 6, 9, 12, 131, 173, 210, 313 |
| Bme1390I | CCNGG | 9 cut(s) | 24, 186, 215, 250, 289, 380, 513, 542, 568 |
| Bme18I | GGWCC | 1 cut(s) | 306 |
| BmgT120I | GGNCC | 3 cut(s) | 252, 306, 570 |
| BmiI | GGNNCC | 4 cut(s) | 247, 307, 546, 572 |
| BmrFI | CCNGG | 9 cut(s) | 24, 186, 215, 250, 289, 380, 513, 542, 568 |
| BmsI | GCATC | 2 cut(s) | 449, 475 |
| BoxI | GACNNNNGTC | 1 cut(s) | 525 |
| BpmI | CTGGAG | 1 cut(s) | 516 |
| BpuMI | CCSGG | 4 cut(s) | 186, 513, 542, 568 |
| BsaHI | GRCGYC | 1 cut(s) | 246 |
| BsaJI | CCNNGG | 4 cut(s) | 249, 288, 379, 411 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 489, 519 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 122, 234, 385, 412, 476 |
| Bse118I | RCCGGY | 3 cut(s) | 234, 254, 453 |
| Bse1I | ACTGG | 2 cut(s) | 58, 499 |
| BseBI | CCWGG | 5 cut(s) | 24, 215, 250, 289, 380 |
| BseDI | CCNNGG | 4 cut(s) | 249, 288, 379, 411 |
| BseGI | GGATG | 2 cut(s) | 401, 490 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 122, 234, 385, 412, 476 |
| BseMII | CTCAG | 1 cut(s) | 382 |
| BseNI | ACTGG | 2 cut(s) | 58, 499 |
| BseRI | GAGGAG | 2 cut(s) | 102, 105 |
| BseXI | GCAGC | 3 cut(s) | 16, 19, 220 |
| BseYI | CCCAGC | 2 cut(s) | 228, 469 |
| Bsh1236I | CGCG | 1 cut(s) | 132 |
| Bsh1285I | CGRYCG | 2 cut(s) | 182, 259 |
| BshFI | GGCC | 7 cut(s) | 22, 108, 129, 174, 254, 444, 571 |
| BshNI | GGYRCC | 1 cut(s) | 245 |
| BsiEI | CGRYCG | 2 cut(s) | 182, 259 |
| BsiSI | CCGG | 7 cut(s) | 186, 235, 255, 454, 512, 542, 568 |
| BslFI | GGGAC | 2 cut(s) | 319, 513 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 122, 234, 385, 412, 476 |
| BsmAI | GTCTC | 2 cut(s) | 113, 513 |
| BsmBI | CGTCTC | 1 cut(s) | 513 |
| BsmFI | GGGAC | 2 cut(s) | 319, 513 |
| BsmI | GAATGC | 1 cut(s) | 380 |
| BsnI | GGCC | 7 cut(s) | 22, 108, 129, 174, 254, 444, 571 |
| Bsp143I | GATC | 4 cut(s) | 26, 59, 398, 508 |
| BspANI | GGCC | 7 cut(s) | 22, 108, 129, 174, 254, 444, 571 |
| BspCNI | CTCAG | 1 cut(s) | 383 |
| BspFNI | CGCG | 1 cut(s) | 132 |
| BspHI | TCATGA | 1 cut(s) | 136 |
| BspLI | GGNNCC | 4 cut(s) | 247, 307, 546, 572 |
| BspMI | ACCTGC | 1 cut(s) | 422 |
| BspPI | GGATC | 4 cut(s) | 34, 67, 393, 503 |
| BspT107I | GGYRCC | 1 cut(s) | 245 |
| BsrBI | CCGCTC | 1 cut(s) | 370 |
| BsrFI | RCCGGY | 3 cut(s) | 234, 254, 453 |
| BsrI | ACTGG | 2 cut(s) | 58, 499 |
| BssAI | RCCGGY | 3 cut(s) | 234, 254, 453 |
| BssECI | CCNNGG | 4 cut(s) | 249, 288, 379, 411 |
| BssMI | GATC | 4 cut(s) | 26, 59, 398, 508 |
| BssNI | GRCGYC | 1 cut(s) | 246 |
| BssT1I | CCWWGG | 1 cut(s) | 411 |
| Bst2UI | CCWGG | 5 cut(s) | 24, 215, 250, 289, 380 |
| BstACI | GRCGYC | 1 cut(s) | 246 |
| BstC8I | GCNNGC | 3 cut(s) | 198, 236, 455 |
| BstDEI | CTNAG | 1 cut(s) | 391 |
| BstF5I | GGATG | 2 cut(s) | 401, 490 |
| BstFNI | CGCG | 1 cut(s) | 132 |
| BstH2I | RGCGCY | 2 cut(s) | 235, 249 |
| BstHHI | GCGC | 4 cut(s) | 97, 134, 234, 248 |
| BstKTI | GATC | 4 cut(s) | 29, 62, 401, 511 |
| BstMAI | GTCTC | 2 cut(s) | 113, 513 |
| BstMBI | GATC | 4 cut(s) | 26, 59, 398, 508 |
| BstMCI | CGRYCG | 2 cut(s) | 182, 259 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 10, 19, 275, 428, 450, 459 |
| BstNI | CCWGG | 5 cut(s) | 24, 215, 250, 289, 380 |
| BstNSI | RCATGY | 1 cut(s) | 273 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 525 |
| BstSCI | CCNGG | 9 cut(s) | 22, 184, 213, 248, 287, 378, 511, 540, 566 |
| BstUI | CGCG | 1 cut(s) | 132 |
| BstV1I | GCAGC | 3 cut(s) | 16, 19, 220 |
| BstX2I | RGATCY | 2 cut(s) | 26, 59 |
| BstYI | RGATCY | 2 cut(s) | 26, 59 |
| BsuRI | GGCC | 7 cut(s) | 22, 108, 129, 174, 254, 444, 571 |
| BtgZI | GCGATG | 1 cut(s) | 472 |
| BtsCI | GGATG | 2 cut(s) | 401, 490 |
| BtsIMutI | CAGTG | 1 cut(s) | 51 |
| BveI | ACCTGC | 1 cut(s) | 422 |
| Cac8I | GCNNGC | 3 cut(s) | 198, 236, 455 |
| CciI | TCATGA | 1 cut(s) | 136 |
| CfoI | GCGC | 4 cut(s) | 97, 134, 234, 248 |
| Cfr10I | RCCGGY | 3 cut(s) | 234, 254, 453 |
| Cfr13I | GGNCC | 3 cut(s) | 252, 306, 570 |
| Csp6I | GTAC | 3 cut(s) | 41, 261, 273 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 274, 309 |
| CviAII | CATG | 3 cut(s) | 38, 137, 270 |
| CviQI | GTAC | 3 cut(s) | 41, 261, 273 |
| DdeI | CTNAG | 1 cut(s) | 391 |
| DinI | GGCGCC | 1 cut(s) | 247 |
| DpnI | GATC | 4 cut(s) | 28, 61, 400, 510 |
| DpnII | GATC | 4 cut(s) | 26, 59, 398, 508 |
| EaeI | YGGCCR | 2 cut(s) | 127, 172 |
| EciI | GGCGGA | 4 cut(s) | 72, 360, 391, 531 |
| Eco130I | CCWWGG | 1 cut(s) | 411 |
| Eco47I | GGWCC | 1 cut(s) | 306 |
| EcoRII | CCWGG | 5 cut(s) | 22, 213, 248, 287, 378 |
| EcoT14I | CCWWGG | 1 cut(s) | 411 |
| EcoT22I | ATGCAT | 1 cut(s) | 271 |
| EgeI | GGCGCC | 1 cut(s) | 247 |
| EheI | GGCGCC | 1 cut(s) | 247 |
| ErhI | CCWWGG | 1 cut(s) | 411 |
| Esp3I | CGTCTC | 1 cut(s) | 513 |
| FaeI | CATG | 3 cut(s) | 41, 140, 273 |
| FaiI | YATR | 6 cut(s) | 39, 67, 138, 271, 318, 408 |
| FaqI | GGGAC | 2 cut(s) | 319, 513 |
| FatI | CATG | 3 cut(s) | 37, 136, 269 |
| FauI | CCCGC | 1 cut(s) | 556 |
| FblI | GTMKAC | 1 cut(s) | 224 |
| Fnu4HI | GCNGC | 7 cut(s) | 5, 8, 11, 130, 172, 209, 312 |
| FokI | GGATG | 2 cut(s) | 408, 497 |
| Fsp4HI | GCNGC | 7 cut(s) | 5, 8, 11, 130, 172, 209, 312 |
| FspBI | CTAG | 1 cut(s) | 412 |
| GlaI | GCGC | 4 cut(s) | 96, 133, 233, 247 |
| GluI | GCNGC | 7 cut(s) | 5, 8, 11, 130, 172, 209, 312 |
| GsaI | CCCAGC | 2 cut(s) | 232, 473 |
| GsuI | CTGGAG | 1 cut(s) | 516 |
| HaeII | RGCGCY | 2 cut(s) | 235, 249 |
| HaeIII | GGCC | 7 cut(s) | 22, 108, 129, 174, 254, 444, 571 |
| HapII | CCGG | 7 cut(s) | 186, 235, 255, 454, 512, 542, 568 |
| HhaI | GCGC | 4 cut(s) | 97, 134, 234, 248 |
| Hin1I | GRCGYC | 1 cut(s) | 246 |
| Hin1II | CATG | 3 cut(s) | 41, 140, 273 |
| Hin6I | GCGC | 4 cut(s) | 95, 132, 232, 246 |
| HinP1I | GCGC | 4 cut(s) | 95, 132, 232, 246 |
| HincII | GTYRAC | 1 cut(s) | 225 |
| HindII | GTYRAC | 1 cut(s) | 225 |
| HinfI | GANTC | 3 cut(s) | 281, 348, 447 |
| HpaII | CCGG | 7 cut(s) | 186, 235, 255, 454, 512, 542, 568 |
| HphI | GGTGA | 1 cut(s) | 26 |
| Hpy166II | GTNNAC | 1 cut(s) | 225 |
| Hpy188III | TCNNGA | 4 cut(s) | 137, 298, 530, 562 |
| Hpy8I | GTNNAC | 1 cut(s) | 225 |
| HpyAV | CCTTC | 1 cut(s) | 98 |
| HpyCH4IV | ACGT | 2 cut(s) | 83, 143 |
| HpyCH4V | TGCA | 3 cut(s) | 269, 462, 488 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 10, 19, 275, 428, 450, 459 |
| HpyF3I | CTNAG | 1 cut(s) | 391 |
| HpySE526I | ACGT | 2 cut(s) | 83, 143 |
| Hsp92I | GRCGYC | 1 cut(s) | 246 |
| Hsp92II | CATG | 3 cut(s) | 41, 140, 273 |
| HspAI | GCGC | 4 cut(s) | 95, 132, 232, 246 |
| KasI | GGCGCC | 1 cut(s) | 245 |
| KroI | GCCGGC | 2 cut(s) | 234, 453 |
| KroNI | GCCGGC | 2 cut(s) | 236, 455 |
| Kzo9I | GATC | 4 cut(s) | 26, 59, 398, 508 |
| LmnI | GCTCC | 2 cut(s) | 92, 497 |
| Lsp1109I | GCAGC | 3 cut(s) | 16, 19, 220 |
| LweI | GCATC | 2 cut(s) | 449, 475 |
| MaeI | CTAG | 1 cut(s) | 412 |
| MaeII | ACGT | 2 cut(s) | 83, 143 |
| MaeIII | GTNAC | 2 cut(s) | 32, 202 |
| MalI | GATC | 4 cut(s) | 28, 61, 400, 510 |
| MbiI | CCGCTC | 1 cut(s) | 370 |
| MboI | GATC | 4 cut(s) | 26, 59, 398, 508 |
| MboII | GAAGA | 1 cut(s) | 54 |
| MflI | RGATCY | 2 cut(s) | 26, 59 |
| Mly113I | GGCGCC | 1 cut(s) | 246 |
| MnlI | CCTC | 6 cut(s) | 80, 83, 143, 331, 386, 496 |
| Mph1103I | ATGCAT | 1 cut(s) | 271 |
| MroNI | GCCGGC | 2 cut(s) | 234, 453 |
| MroXI | GAANNNNTTC | 1 cut(s) | 352 |
| MspA1I | CMGCKG | 1 cut(s) | 278 |
| MspI | CCGG | 7 cut(s) | 186, 235, 255, 454, 512, 542, 568 |
| MspR9I | CCNGG | 9 cut(s) | 24, 186, 215, 250, 289, 380, 513, 542, 568 |
| Mva1269I | GAATGC | 1 cut(s) | 380 |
| MvaI | CCWGG | 5 cut(s) | 24, 215, 250, 289, 380 |
| MvnI | CGCG | 1 cut(s) | 132 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 10, 19, 275, 428, 450, 459 |
| NaeI | GCCGGC | 2 cut(s) | 236, 455 |
| NarI | GGCGCC | 1 cut(s) | 246 |
| NciI | CCSGG | 4 cut(s) | 186, 513, 542, 568 |
| NdeII | GATC | 4 cut(s) | 26, 59, 398, 508 |
| NgoMIV | GCCGGC | 2 cut(s) | 234, 453 |
| NlaIII | CATG | 3 cut(s) | 41, 140, 273 |
| NlaIV | GGNNCC | 4 cut(s) | 247, 307, 546, 572 |
| NmeAIII | GCCGAG | 1 cut(s) | 407 |
| NmuCI | GTSAC | 2 cut(s) | 32, 202 |
| NsiI | ATGCAT | 1 cut(s) | 271 |
| NspI | RCATGY | 1 cut(s) | 273 |
| PagI | TCATGA | 1 cut(s) | 136 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 442, 528 |
| PctI | GAATGC | 1 cut(s) | 380 |
| PdiI | GCCGGC | 2 cut(s) | 236, 455 |
| PdmI | GAANNNNTTC | 1 cut(s) | 352 |
| PfeI | GAWTC | 3 cut(s) | 281, 348, 447 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 476 |
| PkrI | GCNGC | 7 cut(s) | 6, 9, 12, 131, 173, 210, 313 |
| PluTI | GGCGCC | 1 cut(s) | 249 |
| PshAI | GACNNNNGTC | 1 cut(s) | 525 |
| Psp6I | CCWGG | 5 cut(s) | 22, 213, 248, 287, 378 |
| PspFI | CCCAGC | 2 cut(s) | 228, 469 |
| PspGI | CCWGG | 5 cut(s) | 22, 213, 248, 287, 378 |
| PspN4I | GGNNCC | 4 cut(s) | 247, 307, 546, 572 |
| PspPI | GGNCC | 3 cut(s) | 252, 306, 570 |
| PsuI | RGATCY | 2 cut(s) | 26, 59 |
| RsaI | GTAC | 3 cut(s) | 42, 262, 274 |
| RsaNI | GTAC | 3 cut(s) | 41, 261, 273 |
| SalI | GTCGAC | 1 cut(s) | 223 |
| SatI | GCNGC | 7 cut(s) | 5, 8, 11, 130, 172, 209, 312 |
| Sau3AI | GATC | 4 cut(s) | 26, 59, 398, 508 |
| Sau96I | GGNCC | 3 cut(s) | 252, 306, 570 |
| ScrFI | CCNGG | 9 cut(s) | 24, 186, 215, 250, 289, 380, 513, 542, 568 |
| SetI | ASST | 9 cut(s) | 86, 127, 146, 290, 323, 413, 436, 475, 507 |
| SfaNI | GCATC | 2 cut(s) | 449, 475 |
| SfoI | GGCGCC | 1 cut(s) | 247 |
| SinI | GGWCC | 1 cut(s) | 306 |
| SspDI | GGCGCC | 1 cut(s) | 245 |
| SspMI | CTAG | 1 cut(s) | 412 |
| StyD4I | CCNGG | 9 cut(s) | 22, 184, 213, 248, 287, 378, 511, 540, 566 |
| StyI | CCWWGG | 1 cut(s) | 411 |
| TaiI | ACGT | 2 cut(s) | 86, 146 |
| TaqI | TCGA | 2 cut(s) | 224, 372 |
| TaqII | GACCGA | 1 cut(s) | 426 |
| TauI | GCSGC | 4 cut(s) | 13, 132, 174, 314 |
| TfiI | GAWTC | 3 cut(s) | 281, 348, 447 |
| TscAI | CASTG | 1 cut(s) | 58 |
| TseFI | GTSAC | 2 cut(s) | 32, 202 |
| TseI | GCWGC | 3 cut(s) | 4, 7, 208 |
| Tsp45I | GTSAC | 2 cut(s) | 32, 202 |
| TspDTI | ATGAA | 1 cut(s) | 54 |
| TspRI | CASTG | 1 cut(s) | 58 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 476 |
| VpaK11BI | GGWCC | 1 cut(s) | 306 |
| XceI | RCATGY | 1 cut(s) | 273 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 44 |
| XmaJI | CCTAGG | 1 cut(s) | 411 |
| XmiI | GTMKAC | 1 cut(s) | 224 |
| XmnI | GAANNNNTTC | 1 cut(s) | 352 |
| XspI | CTAG | 1 cut(s) | 412 |
| Zsp2I | ATGCAT | 1 cut(s) | 271 |