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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 292 |
| Acc36I | ACCTGC | 1 cut(s) | 292 |
| AccB1I | GGYRCC | 1 cut(s) | 208 |
| AccBSI | CCGCTC | 1 cut(s) | 333 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AciI | CCGC | 7 cut(s) | 145, 239, 275, 308, 331, 365, 394 |
| AclWI | GGATC | 2 cut(s) | 30, 356 |
| AcuI | CTGAAG | 1 cut(s) | 375 |
| AfaI | GTAC | 2 cut(s) | 5, 237 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 85, 197, 348 |
| AflIII | ACRYGT | 1 cut(s) | 104 |
| AjnI | CCWGG | 4 cut(s) | 176, 211, 250, 341 |
| AleI | CACNNNNGTG | 1 cut(s) | 206 |
| AluBI | AGCT | 1 cut(s) | 10 |
| AluI | AGCT | 1 cut(s) | 10 |
| Alw26I | GTCTC | 1 cut(s) | 44 |
| AlwI | GGATC | 2 cut(s) | 30, 356 |
| AoxI | GGCC | 3 cut(s) | 69, 215, 383 |
| ApeKI | GCWGC | 1 cut(s) | 134 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 315 |
| AspLEI | GCGC | 2 cut(s) | 60, 381 |
| AspS9I | GGNCC | 2 cut(s) | 215, 269 |
| AsuC2I | CCSGG | 1 cut(s) | 149 |
| AsuHPI | GGTGA | 1 cut(s) | 371 |
| AvaII | GGWCC | 1 cut(s) | 269 |
| BanI | GGYRCC | 1 cut(s) | 208 |
| BauI | CACGAG | 1 cut(s) | 17 |
| BbsI | GAAGAC | 1 cut(s) | 131 |
| BbvI | GCAGC | 1 cut(s) | 146 |
| BccI | CCATC | 1 cut(s) | 394 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 410 |
| BciT130I | CCWGG | 4 cut(s) | 178, 213, 252, 343 |
| BcnI | CCSGG | 1 cut(s) | 149 |
| BcoDI | GTCTC | 1 cut(s) | 44 |
| BfaI | CTAG | 1 cut(s) | 433 |
| BfuAI | ACCTGC | 1 cut(s) | 292 |
| BisI | GCNGC | 1 cut(s) | 135 |
| BlsI | GCNGC | 1 cut(s) | 136 |
| Bme1390I | CCNGG | 5 cut(s) | 149, 178, 213, 252, 343 |
| Bme18I | GGWCC | 1 cut(s) | 269 |
| BmgT120I | GGNCC | 2 cut(s) | 215, 269 |
| BmiI | GGNNCC | 2 cut(s) | 210, 270 |
| BmrFI | CCNGG | 5 cut(s) | 149, 178, 213, 252, 343 |
| BmsI | GCATC | 2 cut(s) | 219, 412 |
| BpiI | GAAGAC | 1 cut(s) | 131 |
| BpuMI | CCSGG | 1 cut(s) | 149 |
| BsaAI | YACGTR | 1 cut(s) | 170 |
| BsaJI | CCNNGG | 4 cut(s) | 212, 251, 342, 374 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 85, 197, 348 |
| Bse118I | RCCGGY | 2 cut(s) | 197, 217 |
| BseBI | CCWGG | 4 cut(s) | 178, 213, 252, 343 |
| BseDI | CCNNGG | 4 cut(s) | 212, 251, 342, 374 |
| BseGI | GGATG | 1 cut(s) | 386 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 85, 197, 348 |
| BseXI | GCAGC | 1 cut(s) | 146 |
| BseYI | CCCAGC | 2 cut(s) | 191, 390 |
| BsgI | GTGCAG | 1 cut(s) | 305 |
| Bsh1285I | CGRYCG | 1 cut(s) | 145 |
| BshFI | GGCC | 3 cut(s) | 71, 217, 385 |
| BshNI | GGYRCC | 1 cut(s) | 208 |
| BsiEI | CGRYCG | 1 cut(s) | 145 |
| BsiSI | CCGG | 3 cut(s) | 149, 198, 218 |
| BslFI | GGGAC | 1 cut(s) | 282 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 85, 197, 348 |
| BsmAI | GTCTC | 1 cut(s) | 44 |
| BsmBI | CGTCTC | 1 cut(s) | 44 |
| BsmFI | GGGAC | 1 cut(s) | 282 |
| BsnI | GGCC | 3 cut(s) | 71, 217, 385 |
| Bsp143I | GATC | 2 cut(s) | 22, 361 |
| BspACI | CCGC | 7 cut(s) | 145, 239, 275, 308, 331, 365, 394 |
| BspANI | GGCC | 3 cut(s) | 71, 217, 385 |
| BspLI | GGNNCC | 2 cut(s) | 210, 270 |
| BspMI | ACCTGC | 1 cut(s) | 292 |
| BspPI | GGATC | 2 cut(s) | 30, 356 |
| BspT107I | GGYRCC | 1 cut(s) | 208 |
| BsrBI | CCGCTC | 1 cut(s) | 333 |
| BsrFI | RCCGGY | 2 cut(s) | 197, 217 |
| BssAI | RCCGGY | 2 cut(s) | 197, 217 |
| BssECI | CCNNGG | 4 cut(s) | 212, 251, 342, 374 |
| BssMI | GATC | 2 cut(s) | 22, 361 |
| BssSI | CACGAG | 1 cut(s) | 17 |
| Bst2BI | CACGAG | 1 cut(s) | 17 |
| Bst2UI | CCWGG | 4 cut(s) | 178, 213, 252, 343 |
| BstBAI | YACGTR | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 3 cut(s) | 12, 161, 383 |
| BstF5I | GGATG | 1 cut(s) | 386 |
| BstHHI | GCGC | 2 cut(s) | 60, 381 |
| BstKTI | GATC | 2 cut(s) | 25, 364 |
| BstMAI | GTCTC | 1 cut(s) | 44 |
| BstMBI | GATC | 2 cut(s) | 22, 361 |
| BstMCI | CGRYCG | 1 cut(s) | 145 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 238, 391 |
| BstNI | CCWGG | 4 cut(s) | 178, 213, 252, 343 |
| BstSCI | CCNGG | 5 cut(s) | 147, 176, 211, 250, 341 |
| BstV1I | GCAGC | 1 cut(s) | 146 |
| BstV2I | GAAGAC | 1 cut(s) | 131 |
| BstX2I | RGATCY | 1 cut(s) | 22 |
| BstYI | RGATCY | 1 cut(s) | 22 |
| BsuRI | GGCC | 3 cut(s) | 71, 217, 385 |
| BtsCI | GGATG | 1 cut(s) | 386 |
| BveI | ACCTGC | 1 cut(s) | 292 |
| Cac8I | GCNNGC | 3 cut(s) | 12, 161, 383 |
| CfoI | GCGC | 2 cut(s) | 60, 381 |
| Cfr10I | RCCGGY | 2 cut(s) | 197, 217 |
| Cfr13I | GGNCC | 2 cut(s) | 215, 269 |
| Csp6I | GTAC | 2 cut(s) | 4, 236 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 237, 272 |
| CviJI | RGCY | 8 cut(s) | 10, 71, 77, 92, 137, 217, 341, 385 |
| CviKI_1 | RGCY | 8 cut(s) | 10, 71, 77, 92, 137, 217, 341, 385 |
| CviQI | GTAC | 2 cut(s) | 4, 236 |
| DpnI | GATC | 2 cut(s) | 24, 363 |
| DpnII | GATC | 2 cut(s) | 22, 361 |
| EciI | GGCGGA | 3 cut(s) | 264, 323, 354 |
| Eco47I | GGWCC | 1 cut(s) | 269 |
| Eco57I | CTGAAG | 1 cut(s) | 375 |
| EcoO109I | RGGNCCY | 1 cut(s) | 269 |
| EcoRII | CCWGG | 4 cut(s) | 176, 211, 250, 341 |
| Esp3I | CGTCTC | 1 cut(s) | 44 |
| FaiI | YATR | 4 cut(s) | 30, 101, 224, 281 |
| FaqI | GGGAC | 1 cut(s) | 282 |
| FauI | CCCGC | 1 cut(s) | 387 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 1 cut(s) | 135 |
| FokI | GGATG | 1 cut(s) | 373 |
| Fsp4HI | GCNGC | 1 cut(s) | 135 |
| FspBI | CTAG | 1 cut(s) | 433 |
| GlaI | GCGC | 2 cut(s) | 59, 380 |
| GluI | GCNGC | 1 cut(s) | 135 |
| GsaI | CCCAGC | 2 cut(s) | 195, 394 |
| HaeIII | GGCC | 3 cut(s) | 71, 217, 385 |
| HapII | CCGG | 3 cut(s) | 149, 198, 218 |
| HhaI | GCGC | 2 cut(s) | 60, 381 |
| Hin6I | GCGC | 2 cut(s) | 58, 379 |
| HinP1I | GCGC | 2 cut(s) | 58, 379 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 3 cut(s) | 127, 311, 410 |
| HpaII | CCGG | 3 cut(s) | 149, 198, 218 |
| HphI | GGTGA | 1 cut(s) | 371 |
| Hpy166II | GTNNAC | 1 cut(s) | 188 |
| Hpy188I | TCNGA | 2 cut(s) | 337, 405 |
| Hpy8I | GTNNAC | 1 cut(s) | 188 |
| Hpy99I | CGWCG | 1 cut(s) | 422 |
| HpyAV | CCTTC | 1 cut(s) | 350 |
| HpyCH4IV | ACGT | 3 cut(s) | 46, 106, 169 |
| HpyCH4V | TGCA | 4 cut(s) | 14, 232, 322, 425 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 238, 391 |
| HpySE526I | ACGT | 3 cut(s) | 46, 106, 169 |
| HspAI | GCGC | 2 cut(s) | 58, 379 |
| Kzo9I | GATC | 2 cut(s) | 22, 361 |
| LmnI | GCTCC | 1 cut(s) | 55 |
| Lsp1109I | GCAGC | 1 cut(s) | 146 |
| LweI | GCATC | 2 cut(s) | 219, 412 |
| MaeI | CTAG | 1 cut(s) | 433 |
| MaeII | ACGT | 3 cut(s) | 46, 106, 169 |
| MaeIII | GTNAC | 1 cut(s) | 165 |
| MalI | GATC | 2 cut(s) | 24, 363 |
| MbiI | CCGCTC | 1 cut(s) | 333 |
| MboI | GATC | 2 cut(s) | 22, 361 |
| MboII | GAAGA | 2 cut(s) | 17, 136 |
| MflI | RGATCY | 1 cut(s) | 22 |
| MlyI | GAGTC | 1 cut(s) | 121 |
| MnlI | CCTC | 5 cut(s) | 106, 282, 294, 342, 369 |
| MroXI | GAANNNNTTC | 1 cut(s) | 315 |
| MslI | CAYNNNNRTG | 2 cut(s) | 206, 227 |
| MspA1I | CMGCKG | 2 cut(s) | 241, 394 |
| MspI | CCGG | 3 cut(s) | 149, 198, 218 |
| MspR9I | CCNGG | 5 cut(s) | 149, 178, 213, 252, 343 |
| MvaI | CCWGG | 4 cut(s) | 178, 213, 252, 343 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 238, 391 |
| NciI | CCSGG | 1 cut(s) | 149 |
| NdeII | GATC | 2 cut(s) | 22, 361 |
| NlaIV | GGNNCC | 2 cut(s) | 210, 270 |
| NmeAIII | GCCGAG | 1 cut(s) | 399 |
| NmuCI | GTSAC | 1 cut(s) | 165 |
| OliI | CACNNNNGTG | 1 cut(s) | 206 |
| PaqCI | CACCTGC | 1 cut(s) | 292 |
| PdmI | GAANNNNTTC | 1 cut(s) | 315 |
| PfeI | GAWTC | 2 cut(s) | 311, 410 |
| PkrI | GCNGC | 1 cut(s) | 136 |
| PleI | GAGTC | 1 cut(s) | 121 |
| PpsI | GAGTC | 1 cut(s) | 121 |
| Ppu21I | YACGTR | 1 cut(s) | 170 |
| PpuMI | RGGWCCY | 1 cut(s) | 269 |
| Psp5II | RGGWCCY | 1 cut(s) | 269 |
| Psp6I | CCWGG | 4 cut(s) | 176, 211, 250, 341 |
| PspFI | CCCAGC | 2 cut(s) | 191, 390 |
| PspGI | CCWGG | 4 cut(s) | 176, 211, 250, 341 |
| PspN4I | GGNNCC | 2 cut(s) | 210, 270 |
| PspPI | GGNCC | 2 cut(s) | 215, 269 |
| PspPPI | RGGWCCY | 1 cut(s) | 269 |
| PsuI | RGATCY | 1 cut(s) | 22 |
| RsaI | GTAC | 2 cut(s) | 5, 237 |
| RsaNI | GTAC | 2 cut(s) | 4, 236 |
| RseI | CAYNNNNRTG | 2 cut(s) | 206, 227 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SatI | GCNGC | 1 cut(s) | 135 |
| Sau3AI | GATC | 2 cut(s) | 22, 361 |
| Sau96I | GGNCC | 2 cut(s) | 215, 269 |
| SchI | GAGTC | 1 cut(s) | 121 |
| ScrFI | CCNGG | 5 cut(s) | 149, 178, 213, 252, 343 |
| SfaNI | GCATC | 2 cut(s) | 219, 412 |
| SinI | GGWCC | 1 cut(s) | 269 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 206, 227 |
| SsiI | CCGC | 7 cut(s) | 145, 239, 275, 308, 331, 365, 394 |
| SspMI | CTAG | 1 cut(s) | 433 |
| StyD4I | CCNGG | 5 cut(s) | 147, 176, 211, 250, 341 |
| TaiI | ACGT | 3 cut(s) | 49, 109, 172 |
| TaqI | TCGA | 2 cut(s) | 111, 187 |
| TaqII | GACCGA | 1 cut(s) | 422 |
| TfiI | GAWTC | 2 cut(s) | 311, 410 |
| TseFI | GTSAC | 1 cut(s) | 165 |
| TseI | GCWGC | 1 cut(s) | 134 |
| Tsp45I | GTSAC | 1 cut(s) | 165 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 68 |
| VpaK11BI | GGWCC | 1 cut(s) | 269 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XmnI | GAANNNNTTC | 1 cut(s) | 315 |
| XspI | CTAG | 1 cut(s) | 433 |