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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 328 |
| AccBSI | CCGCTC | 1 cut(s) | 219 |
| AccII | CGCG | 1 cut(s) | 300 |
| AciI | CCGC | 7 cut(s) | 32, 55, 65, 110, 156, 185, 219 |
| AclI | AACGTT | 1 cut(s) | 117 |
| AfaI | GTAC | 1 cut(s) | 237 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 157, 238, 283, 337 |
| AjnI | CCWGG | 2 cut(s) | 165, 231 |
| AluBI | AGCT | 2 cut(s) | 225, 262 |
| AluI | AGCT | 2 cut(s) | 225, 262 |
| AoxI | GGCC | 3 cut(s) | 168, 321, 327 |
| ApeKI | GCWGC | 3 cut(s) | 222, 225, 248 |
| AspS9I | GGNCC | 2 cut(s) | 57, 328 |
| AvaII | GGWCC | 1 cut(s) | 57 |
| BbvI | GCAGC | 3 cut(s) | 212, 234, 235 |
| BciT130I | CCWGG | 2 cut(s) | 167, 233 |
| BfaI | CTAG | 3 cut(s) | 24, 153, 269 |
| BfmI | CTRYAG | 1 cut(s) | 198 |
| BfuAI | ACCTGC | 1 cut(s) | 328 |
| BisI | GCNGC | 4 cut(s) | 220, 223, 226, 249 |
| BlpI | GCTNAGC | 1 cut(s) | 210 |
| BlsI | GCNGC | 4 cut(s) | 221, 224, 227, 250 |
| Bme1390I | CCNGG | 2 cut(s) | 167, 233 |
| Bme18I | GGWCC | 1 cut(s) | 57 |
| BmgT120I | GGNCC | 2 cut(s) | 57, 328 |
| BmiI | GGNNCC | 1 cut(s) | 162 |
| BmrFI | CCNGG | 2 cut(s) | 167, 233 |
| Bpu10I | CCTNAGC | 1 cut(s) | 44 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 210 |
| BsaJI | CCNNGG | 4 cut(s) | 165, 204, 231, 324 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 157, 238, 283, 337 |
| Bse1I | ACTGG | 2 cut(s) | 143, 171 |
| BseBI | CCWGG | 2 cut(s) | 167, 233 |
| BseDI | CCNNGG | 4 cut(s) | 165, 204, 231, 324 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 157, 238, 283, 337 |
| BseMII | CTCAG | 1 cut(s) | 224 |
| BseNI | ACTGG | 2 cut(s) | 143, 171 |
| BseXI | GCAGC | 3 cut(s) | 212, 234, 235 |
| Bsh1236I | CGCG | 1 cut(s) | 300 |
| BshFI | GGCC | 3 cut(s) | 170, 323, 329 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 157, 238, 283, 337 |
| BsnI | GGCC | 3 cut(s) | 170, 323, 329 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 210 |
| BspACI | CCGC | 7 cut(s) | 32, 55, 65, 110, 156, 185, 219 |
| BspANI | GGCC | 3 cut(s) | 170, 323, 329 |
| BspCNI | CTCAG | 1 cut(s) | 223 |
| BspFNI | CGCG | 1 cut(s) | 300 |
| BspLI | GGNNCC | 1 cut(s) | 162 |
| BspMI | ACCTGC | 1 cut(s) | 328 |
| BsrBI | CCGCTC | 1 cut(s) | 219 |
| BsrI | ACTGG | 2 cut(s) | 143, 171 |
| BssECI | CCNNGG | 4 cut(s) | 165, 204, 231, 324 |
| BssT1I | CCWWGG | 1 cut(s) | 204 |
| Bst2UI | CCWGG | 2 cut(s) | 167, 233 |
| BstC8I | GCNNGC | 3 cut(s) | 53, 264, 302 |
| BstDEI | CTNAG | 2 cut(s) | 44, 210 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 236, 281 |
| BstFNI | CGCG | 1 cut(s) | 300 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 225 |
| BstNI | CCWGG | 2 cut(s) | 167, 233 |
| BstSCI | CCNGG | 2 cut(s) | 165, 231 |
| BstSFI | CTRYAG | 1 cut(s) | 198 |
| BstUI | CGCG | 1 cut(s) | 300 |
| BstV1I | GCAGC | 3 cut(s) | 212, 234, 235 |
| BsuRI | GGCC | 3 cut(s) | 170, 323, 329 |
| BveI | ACCTGC | 1 cut(s) | 328 |
| Cac8I | GCNNGC | 3 cut(s) | 53, 264, 302 |
| Cfr13I | GGNCC | 2 cut(s) | 57, 328 |
| Csp6I | GTAC | 1 cut(s) | 236 |
| CviAII | CATG | 2 cut(s) | 125, 305 |
| CviJI | RGCY | 9 cut(s) | 6, 170, 203, 209, 214, 225, 262, 323, 329 |
| CviKI_1 | RGCY | 9 cut(s) | 6, 170, 203, 209, 214, 225, 262, 323, 329 |
| CviQI | GTAC | 1 cut(s) | 236 |
| DdeI | CTNAG | 2 cut(s) | 44, 210 |
| Eco130I | CCWWGG | 1 cut(s) | 204 |
| Eco47I | GGWCC | 1 cut(s) | 57 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 236, 281 |
| EcoRII | CCWGG | 2 cut(s) | 165, 231 |
| EcoT14I | CCWWGG | 1 cut(s) | 204 |
| ErhI | CCWWGG | 1 cut(s) | 204 |
| FaeI | CATG | 2 cut(s) | 128, 308 |
| FaiI | YATR | 5 cut(s) | 15, 126, 254, 258, 306 |
| FatI | CATG | 2 cut(s) | 124, 304 |
| FauI | CCCGC | 4 cut(s) | 25, 72, 149, 192 |
| Fnu4HI | GCNGC | 4 cut(s) | 220, 223, 226, 249 |
| Fsp4HI | GCNGC | 4 cut(s) | 220, 223, 226, 249 |
| FspBI | CTAG | 3 cut(s) | 24, 153, 269 |
| GluI | GCNGC | 4 cut(s) | 220, 223, 226, 249 |
| HaeIII | GGCC | 3 cut(s) | 170, 323, 329 |
| Hin1II | CATG | 2 cut(s) | 128, 308 |
| HincII | GTYRAC | 3 cut(s) | 115, 121, 148 |
| HindII | GTYRAC | 3 cut(s) | 115, 121, 148 |
| HinfI | GANTC | 1 cut(s) | 190 |
| HpaI | GTTAAC | 2 cut(s) | 115, 121 |
| Hpy166II | GTNNAC | 3 cut(s) | 115, 121, 148 |
| Hpy8I | GTNNAC | 3 cut(s) | 115, 121, 148 |
| HpyAV | CCTTC | 1 cut(s) | 287 |
| HpyCH4IV | ACGT | 2 cut(s) | 117, 346 |
| HpyCH4V | TGCA | 1 cut(s) | 128 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 225 |
| HpyF3I | CTNAG | 2 cut(s) | 44, 210 |
| HpySE526I | ACGT | 2 cut(s) | 117, 346 |
| Hsp92II | CATG | 2 cut(s) | 128, 308 |
| KspAI | GTTAAC | 2 cut(s) | 115, 121 |
| LmnI | GCTCC | 1 cut(s) | 48 |
| Lsp1109I | GCAGC | 3 cut(s) | 212, 234, 235 |
| MaeI | CTAG | 3 cut(s) | 24, 153, 269 |
| MaeII | ACGT | 2 cut(s) | 117, 346 |
| MaeIII | GTNAC | 1 cut(s) | 173 |
| MbiI | CCGCTC | 1 cut(s) | 219 |
| MlyI | GAGTC | 1 cut(s) | 184 |
| MnlI | CCTC | 1 cut(s) | 319 |
| MseI | TTAA | 3 cut(s) | 102, 114, 120 |
| MslI | CAYNNNNRTG | 1 cut(s) | 12 |
| MspA1I | CMGCKG | 2 cut(s) | 110, 225 |
| MspR9I | CCNGG | 2 cut(s) | 167, 233 |
| MvaI | CCWGG | 2 cut(s) | 167, 233 |
| MvnI | CGCG | 1 cut(s) | 300 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 225 |
| NlaIII | CATG | 2 cut(s) | 128, 308 |
| NlaIV | GGNNCC | 1 cut(s) | 162 |
| NmeAIII | GCCGAG | 2 cut(s) | 240, 349 |
| PkrI | GCNGC | 4 cut(s) | 221, 224, 227, 250 |
| PleI | GAGTC | 1 cut(s) | 184 |
| PpsI | GAGTC | 1 cut(s) | 184 |
| Psp1406I | AACGTT | 1 cut(s) | 117 |
| Psp6I | CCWGG | 2 cut(s) | 165, 231 |
| PspGI | CCWGG | 2 cut(s) | 165, 231 |
| PspN4I | GGNNCC | 1 cut(s) | 162 |
| PspPI | GGNCC | 2 cut(s) | 57, 328 |
| PvuII | CAGCTG | 1 cut(s) | 225 |
| RsaI | GTAC | 1 cut(s) | 237 |
| RsaNI | GTAC | 1 cut(s) | 236 |
| RseI | CAYNNNNRTG | 1 cut(s) | 12 |
| SaqAI | TTAA | 3 cut(s) | 102, 114, 120 |
| SatI | GCNGC | 4 cut(s) | 220, 223, 226, 249 |
| Sau96I | GGNCC | 2 cut(s) | 57, 328 |
| SchI | GAGTC | 1 cut(s) | 184 |
| ScrFI | CCNGG | 2 cut(s) | 167, 233 |
| SetI | ASST | 7 cut(s) | 120, 180, 227, 264, 279, 342, 349 |
| SfcI | CTRYAG | 1 cut(s) | 198 |
| SinI | GGWCC | 1 cut(s) | 57 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 12 |
| SsiI | CCGC | 7 cut(s) | 32, 55, 65, 110, 156, 185, 219 |
| SspMI | CTAG | 3 cut(s) | 24, 153, 269 |
| StyD4I | CCNGG | 2 cut(s) | 165, 231 |
| StyI | CCWWGG | 1 cut(s) | 204 |
| TaiI | ACGT | 2 cut(s) | 120, 349 |
| TauI | GCSGC | 1 cut(s) | 222 |
| Tru1I | TTAA | 3 cut(s) | 102, 114, 120 |
| Tru9I | TTAA | 3 cut(s) | 102, 114, 120 |
| TseI | GCWGC | 3 cut(s) | 222, 225, 248 |
| TspDTI | ATGAA | 1 cut(s) | 321 |
| VpaK11BI | GGWCC | 1 cut(s) | 57 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 236, 281 |
| XspI | CTAG | 3 cut(s) | 24, 153, 269 |