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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 76 |
| Acc36I | ACCTGC | 1 cut(s) | 76 |
| Acc65I | GGTACC | 1 cut(s) | 208 |
| AccB1I | GGYRCC | 1 cut(s) | 208 |
| AccBSI | CCGCTC | 1 cut(s) | 327 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AccII | CGCG | 1 cut(s) | 169 |
| AciI | CCGC | 5 cut(s) | 20, 169, 239, 302, 325 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AfaI | GTAC | 4 cut(s) | 47, 210, 216, 237 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 31, 85, 197, 253, 289 |
| Alw21I | GWGCWC | 1 cut(s) | 176 |
| AoxI | GGCC | 3 cut(s) | 90, 262, 339 |
| ApaI | GGGCCC | 1 cut(s) | 266 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| Asp718I | GGTACC | 1 cut(s) | 208 |
| AspLEI | GCGC | 2 cut(s) | 60, 130 |
| AspS9I | GGNCC | 4 cut(s) | 22, 76, 262, 263 |
| AsuHPI | GGTGA | 1 cut(s) | 268 |
| AvaII | GGWCC | 2 cut(s) | 22, 76 |
| BaeGI | GKGCMC | 1 cut(s) | 266 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BanI | GGYRCC | 1 cut(s) | 208 |
| BanII | GRGCYC | 1 cut(s) | 266 |
| Bbv12I | GWGCWC | 1 cut(s) | 176 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BccI | CCATC | 2 cut(s) | 253, 283 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| BfuAI | ACCTGC | 1 cut(s) | 76 |
| BisI | GCNGC | 1 cut(s) | 325 |
| BlsI | GCNGC | 1 cut(s) | 326 |
| Bme18I | GGWCC | 2 cut(s) | 22, 76 |
| BmgT120I | GGNCC | 4 cut(s) | 22, 76, 262, 263 |
| BmiI | GGNNCC | 2 cut(s) | 210, 264 |
| BmsI | GCATC | 1 cut(s) | 219 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 31, 85, 197, 253, 289 |
| Bse1I | ACTGG | 1 cut(s) | 218 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 31, 85, 197, 253, 289 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseNI | ACTGG | 1 cut(s) | 218 |
| BseRI | GAGGAG | 1 cut(s) | 261 |
| BseSI | GKGCMC | 1 cut(s) | 266 |
| Bsh1236I | CGCG | 1 cut(s) | 169 |
| BshFI | GGCC | 3 cut(s) | 92, 264, 341 |
| BshNI | GGYRCC | 1 cut(s) | 208 |
| BsiHKAI | GWGCWC | 1 cut(s) | 176 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 31, 85, 197, 253, 289 |
| BsnI | GGCC | 3 cut(s) | 92, 264, 341 |
| Bsp120I | GGGCCC | 1 cut(s) | 262 |
| Bsp1286I | GDGCHC | 2 cut(s) | 176, 266 |
| BspACI | CCGC | 5 cut(s) | 20, 169, 239, 302, 325 |
| BspANI | GGCC | 3 cut(s) | 92, 264, 341 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspFNI | CGCG | 1 cut(s) | 169 |
| BspLI | GGNNCC | 2 cut(s) | 210, 264 |
| BspMI | ACCTGC | 1 cut(s) | 76 |
| BspT107I | GGYRCC | 1 cut(s) | 208 |
| BsrBI | CCGCTC | 1 cut(s) | 327 |
| BsrI | ACTGG | 1 cut(s) | 218 |
| BstC8I | GCNNGC | 2 cut(s) | 161, 199 |
| BstDEI | CTNAG | 2 cut(s) | 72, 336 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstFNI | CGCG | 1 cut(s) | 169 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 2 cut(s) | 60, 130 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 238, 338 |
| BstSLI | GKGCMC | 1 cut(s) | 266 |
| BstUI | CGCG | 1 cut(s) | 169 |
| BsuRI | GGCC | 3 cut(s) | 92, 264, 341 |
| BveI | ACCTGC | 1 cut(s) | 76 |
| Cac8I | GCNNGC | 2 cut(s) | 161, 199 |
| CfoI | GCGC | 2 cut(s) | 60, 130 |
| Cfr13I | GGNCC | 4 cut(s) | 22, 76, 262, 263 |
| Csp6I | GTAC | 4 cut(s) | 46, 209, 215, 236 |
| CviAII | CATG | 2 cut(s) | 94, 279 |
| CviJI | RGCY | 7 cut(s) | 14, 71, 92, 264, 313, 324, 341 |
| CviKI_1 | RGCY | 7 cut(s) | 14, 71, 92, 264, 313, 324, 341 |
| CviQI | GTAC | 4 cut(s) | 46, 209, 215, 236 |
| DdeI | CTNAG | 2 cut(s) | 72, 336 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| EciI | GGCGGA | 2 cut(s) | 35, 317 |
| Eco24I | GRGCYC | 1 cut(s) | 266 |
| Eco47I | GGWCC | 2 cut(s) | 22, 76 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoT38I | GRGCYC | 1 cut(s) | 266 |
| FaeI | CATG | 2 cut(s) | 97, 282 |
| FaiI | YATR | 7 cut(s) | 6, 62, 95, 183, 224, 280, 349 |
| FatI | CATG | 2 cut(s) | 93, 278 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 1 cut(s) | 325 |
| FriOI | GRGCYC | 1 cut(s) | 266 |
| Fsp4HI | GCNGC | 1 cut(s) | 325 |
| GlaI | GCGC | 2 cut(s) | 59, 129 |
| GluI | GCNGC | 1 cut(s) | 325 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 3 cut(s) | 92, 264, 341 |
| HhaI | GCGC | 2 cut(s) | 60, 130 |
| Hin1II | CATG | 2 cut(s) | 97, 282 |
| Hin6I | GCGC | 2 cut(s) | 58, 128 |
| HinP1I | GCGC | 2 cut(s) | 58, 128 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 1 cut(s) | 305 |
| HphI | GGTGA | 1 cut(s) | 268 |
| Hpy166II | GTNNAC | 1 cut(s) | 188 |
| Hpy8I | GTNNAC | 1 cut(s) | 188 |
| HpyAV | CCTTC | 1 cut(s) | 61 |
| HpyCH4V | TGCA | 2 cut(s) | 197, 232 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 238, 338 |
| HpyF3I | CTNAG | 2 cut(s) | 72, 336 |
| Hsp92II | CATG | 2 cut(s) | 97, 282 |
| HspAI | GCGC | 2 cut(s) | 58, 128 |
| KpnI | GGTACC | 1 cut(s) | 212 |
| LmnI | GCTCC | 1 cut(s) | 171 |
| LpnPI | CCDG | 8 cut(s) | 25, 71, 116, 183, 231, 264, 281, 334 |
| LweI | GCATC | 1 cut(s) | 219 |
| MbiI | CCGCTC | 1 cut(s) | 327 |
| MboII | GAAGA | 1 cut(s) | 146 |
| MhlI | GDGCHC | 2 cut(s) | 176, 266 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluCI | AATT | 2 cut(s) | 107, 116 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MnlI | CCTC | 6 cut(s) | 35, 67, 247, 279, 282, 292 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MseI | TTAA | 1 cut(s) | 119 |
| MslI | CAYNNNNRTG | 2 cut(s) | 98, 227 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspA1I | CMGCKG | 1 cut(s) | 241 |
| MvnI | CGCG | 1 cut(s) | 169 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 238, 338 |
| NlaIII | CATG | 2 cut(s) | 97, 282 |
| NlaIV | GGNNCC | 2 cut(s) | 210, 264 |
| NmeAIII | GCCGAG | 1 cut(s) | 308 |
| PaqCI | CACCTGC | 1 cut(s) | 76 |
| PfeI | GAWTC | 1 cut(s) | 305 |
| PkrI | GCNGC | 1 cut(s) | 326 |
| PspN4I | GGNNCC | 2 cut(s) | 210, 264 |
| PspOMI | GGGCCC | 1 cut(s) | 262 |
| PspPI | GGNCC | 4 cut(s) | 22, 76, 262, 263 |
| RsaI | GTAC | 4 cut(s) | 47, 210, 216, 237 |
| RsaNI | GTAC | 4 cut(s) | 46, 209, 215, 236 |
| RseI | CAYNNNNRTG | 2 cut(s) | 98, 227 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SaqAI | TTAA | 1 cut(s) | 119 |
| SatI | GCNGC | 1 cut(s) | 325 |
| Sau96I | GGNCC | 4 cut(s) | 22, 76, 262, 263 |
| SduI | GDGCHC | 2 cut(s) | 176, 266 |
| SetI | ASST | 5 cut(s) | 27, 78, 90, 258, 271 |
| SfaNI | GCATC | 1 cut(s) | 219 |
| SinI | GGWCC | 2 cut(s) | 22, 76 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 98, 227 |
| Sse9I | AATT | 2 cut(s) | 107, 116 |
| SsiI | CCGC | 5 cut(s) | 20, 169, 239, 302, 325 |
| TaqI | TCGA | 2 cut(s) | 111, 187 |
| TasI | AATT | 2 cut(s) | 107, 116 |
| TauI | GCSGC | 1 cut(s) | 327 |
| TfiI | GAWTC | 1 cut(s) | 305 |
| Tru1I | TTAA | 1 cut(s) | 119 |
| Tru9I | TTAA | 1 cut(s) | 119 |
| TspDTI | ATGAA | 1 cut(s) | 129 |
| VpaK11BI | GGWCC | 2 cut(s) | 22, 76 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XmiI | GTMKAC | 1 cut(s) | 187 |