Uncharacterized protein K02A2.6-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 394 |
| Acc65I | GGTACC | 1 cut(s) | 406 |
| AccB1I | GGYRCC | 5 cut(s) | 365, 406, 667, 925, 1061 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 610, 850 |
| AccBSI | CCGCTC | 3 cut(s) | 504, 685, 1045 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 4 cut(s) | 74, 883, 892, 1142 |
| AccIII | TCCGGA | 1 cut(s) | 997 |
| AclWI | GGATC | 6 cut(s) | 201, 637, 782, 944, 957, 1067 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcsI | RAATTY | 1 cut(s) | 903 |
| AcuI | CTGAAG | 3 cut(s) | 48, 264, 546 |
| AcyI | GRCGYC | 2 cut(s) | 668, 1062 |
| AfaI | GTAC | 3 cut(s) | 176, 396, 408 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 508, 610, 850 |
| AflIII | ACRYGT | 2 cut(s) | 275, 1002 |
| AgsI | TTSAA | 3 cut(s) | 814, 870, 1012 |
| AjiI | CACGTC | 1 cut(s) | 1005 |
| AjnI | CCWGG | 5 cut(s) | 347, 382, 700, 1150, 1299 |
| AluBI | AGCT | 3 cut(s) | 181, 850, 1183 |
| AluI | AGCT | 3 cut(s) | 181, 850, 1183 |
| Alw21I | GWGCWC | 2 cut(s) | 94, 1038 |
| AlwI | GGATC | 6 cut(s) | 201, 637, 782, 944, 957, 1067 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 850 |
| Aor13HI | TCCGGA | 1 cut(s) | 997 |
| AoxI | GGCC | 7 cut(s) | 108, 154, 306, 386, 554, 703, 1227 |
| ApeKI | GCWGC | 4 cut(s) | 138, 141, 716, 847 |
| ApoI | RAATTY | 1 cut(s) | 903 |
| Asp718I | GGTACC | 1 cut(s) | 406 |
| AspA2I | CCTAGG | 1 cut(s) | 953 |
| AspLEI | GCGC | 6 cut(s) | 231, 670, 883, 892, 894, 1064 |
| AspS9I | GGNCC | 5 cut(s) | 386, 440, 704, 1134, 1227 |
| AsuC2I | CCSGG | 6 cut(s) | 107, 117, 363, 647, 665, 1250 |
| AsuHPI | GGTGA | 2 cut(s) | 703, 814 |
| AsuNHI | GCTAGC | 1 cut(s) | 181 |
| AvaII | GGWCC | 2 cut(s) | 440, 1134 |
| AvrII | CCTAGG | 1 cut(s) | 953 |
| AxyI | CCTNAGG | 1 cut(s) | 1164 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 390, 423 |
| BamHI | GGATCC | 1 cut(s) | 949 |
| BanI | GGYRCC | 5 cut(s) | 365, 406, 667, 925, 1061 |
| BauI | CACGAG | 1 cut(s) | 855 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| Bbv12I | GWGCWC | 2 cut(s) | 94, 1038 |
| BbvI | GCAGC | 4 cut(s) | 150, 153, 703, 859 |
| BccI | CCATC | 4 cut(s) | 59, 157, 1196, 1217 |
| BceAI | ACGGC | 4 cut(s) | 541, 578, 790, 1106 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 581, 615 |
| BciT130I | CCWGG | 5 cut(s) | 349, 384, 702, 1152, 1301 |
| BcnI | CCSGG | 6 cut(s) | 107, 117, 363, 647, 665, 1250 |
| BfaI | CTAG | 3 cut(s) | 182, 513, 954 |
| BfmI | CTRYAG | 3 cut(s) | 725, 876, 1277 |
| BfoI | RGCGCY | 2 cut(s) | 671, 1065 |
| BfuAI | ACCTGC | 1 cut(s) | 394 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 562 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 7 cut(s) | 121, 139, 142, 717, 848, 895, 1119 |
| BlnI | CCTAGG | 1 cut(s) | 953 |
| BlsI | GCNGC | 7 cut(s) | 122, 140, 143, 718, 849, 896, 1120 |
| Bme18I | GGWCC | 2 cut(s) | 440, 1134 |
| BmgBI | CACGTC | 1 cut(s) | 1005 |
| BmgT120I | GGNCC | 5 cut(s) | 386, 440, 704, 1134, 1227 |
| BmiI | GGNNCC | 9 cut(s) | 367, 408, 441, 669, 706, 927, 951, 987, 1063 |
| BmrI | ACTGGG | 2 cut(s) | 1060, 1309 |
| BmsI | GCATC | 4 cut(s) | 583, 609, 752, 1032 |
| BmtI | GCTAGC | 1 cut(s) | 185 |
| BmuI | ACTGGG | 2 cut(s) | 1060, 1309 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| BpmI | CTGGAG | 2 cut(s) | 323, 650 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1041 |
| BpuMI | CCSGG | 6 cut(s) | 107, 117, 363, 647, 665, 1250 |
| BsaAI | YACGTR | 2 cut(s) | 341, 1109 |
| BsaHI | GRCGYC | 2 cut(s) | 668, 1062 |
| BsaJI | CCNNGG | 6 cut(s) | 72, 105, 115, 383, 545, 953 |
| BsaWI | WCCGGW | 1 cut(s) | 997 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 623, 653, 1251, 1281 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 508, 610, 850 |
| Bse118I | RCCGGY | 3 cut(s) | 368, 587, 988 |
| Bse1I | ACTGG | 5 cut(s) | 192, 389, 633, 1066, 1315 |
| Bse21I | CCTNAGG | 1 cut(s) | 1164 |
| BseAI | TCCGGA | 1 cut(s) | 997 |
| BseBI | CCWGG | 5 cut(s) | 349, 384, 702, 1152, 1301 |
| BseDI | CCNNGG | 6 cut(s) | 72, 105, 115, 383, 545, 953 |
| BseGI | GGATG | 4 cut(s) | 51, 624, 964, 1293 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 508, 610, 850 |
| BseMII | CTCAG | 5 cut(s) | 414, 689, 1032, 1041, 1155 |
| BseNI | ACTGG | 5 cut(s) | 192, 389, 633, 1066, 1315 |
| BsePI | GCGCGC | 1 cut(s) | 890 |
| BseRI | GAGGAG | 5 cut(s) | 236, 239, 977, 980, 1277 |
| BseXI | GCAGC | 4 cut(s) | 150, 153, 703, 859 |
| BseYI | CCCAGC | 1 cut(s) | 319 |
| Bsh1236I | CGCG | 4 cut(s) | 74, 883, 892, 1142 |
| BshFI | GGCC | 7 cut(s) | 110, 156, 308, 388, 556, 705, 1229 |
| BshNI | GGYRCC | 5 cut(s) | 365, 406, 667, 925, 1061 |
| BsiHKAI | GWGCWC | 2 cut(s) | 94, 1038 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 508, 610, 850 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsnI | GGCC | 7 cut(s) | 110, 156, 308, 388, 556, 705, 1229 |
| Bsp1286I | GDGCHC | 2 cut(s) | 94, 1038 |
| Bsp13I | TCCGGA | 1 cut(s) | 997 |
| Bsp143I | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| BspANI | GGCC | 7 cut(s) | 110, 156, 308, 388, 556, 705, 1229 |
| BspCNI | CTCAG | 5 cut(s) | 415, 688, 1033, 1040, 1156 |
| BspEI | TCCGGA | 1 cut(s) | 997 |
| BspFNI | CGCG | 4 cut(s) | 74, 883, 892, 1142 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 9 cut(s) | 367, 408, 441, 669, 706, 927, 951, 987, 1063 |
| BspMAI | CTGCAG | 1 cut(s) | 729 |
| BspMI | ACCTGC | 1 cut(s) | 394 |
| BspOI | GCTAGC | 1 cut(s) | 185 |
| BspPI | GGATC | 6 cut(s) | 201, 637, 782, 944, 957, 1067 |
| BspQI | GCTCTTC | 2 cut(s) | 24, 692 |
| BspT107I | GGYRCC | 5 cut(s) | 365, 406, 667, 925, 1061 |
| BsrBI | CCGCTC | 3 cut(s) | 504, 685, 1045 |
| BsrFI | RCCGGY | 3 cut(s) | 368, 587, 988 |
| BsrI | ACTGG | 5 cut(s) | 192, 389, 633, 1066, 1315 |
| BssAI | RCCGGY | 3 cut(s) | 368, 587, 988 |
| BssECI | CCNNGG | 6 cut(s) | 72, 105, 115, 383, 545, 953 |
| BssHII | GCGCGC | 1 cut(s) | 890 |
| BssMI | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| BssNI | GRCGYC | 2 cut(s) | 668, 1062 |
| BssSI | CACGAG | 1 cut(s) | 855 |
| BssT1I | CCWWGG | 2 cut(s) | 545, 953 |
| Bst2BI | CACGAG | 1 cut(s) | 855 |
| Bst2UI | CCWGG | 5 cut(s) | 349, 384, 702, 1152, 1301 |
| Bst4CI | ACNGT | 4 cut(s) | 11, 877, 913, 1051 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 692 |
| BstACI | GRCGYC | 2 cut(s) | 668, 1062 |
| BstBAI | YACGTR | 2 cut(s) | 341, 1109 |
| BstC8I | GCNNGC | 9 cut(s) | 36, 48, 183, 332, 589, 729, 845, 852, 892 |
| BstDEI | CTNAG | 5 cut(s) | 423, 675, 1027, 1041, 1164 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstEII | GGTNACC | 1 cut(s) | 820 |
| BstF5I | GGATG | 4 cut(s) | 51, 624, 964, 1293 |
| BstFNI | CGCG | 4 cut(s) | 74, 883, 892, 1142 |
| BstH2I | RGCGCY | 2 cut(s) | 671, 1065 |
| BstHHI | GCGC | 6 cut(s) | 231, 670, 883, 892, 894, 1064 |
| BstKTI | GATC | 6 cut(s) | 163, 196, 645, 790, 952, 1075 |
| BstMBI | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 320, 562, 584, 593, 887, 889, 1042, 1127 |
| BstNI | CCWGG | 5 cut(s) | 349, 384, 702, 1152, 1301 |
| BstNSI | RCATGY | 1 cut(s) | 1150 |
| BstPI | GGTNACC | 1 cut(s) | 820 |
| BstSFI | CTRYAG | 3 cut(s) | 725, 876, 1277 |
| BstUI | CGCG | 4 cut(s) | 74, 883, 892, 1142 |
| BstV1I | GCAGC | 4 cut(s) | 150, 153, 703, 859 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 4 cut(s) | 160, 193, 949, 1072 |
| BstYI | RGATCY | 4 cut(s) | 160, 193, 949, 1072 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1164 |
| BsuRI | GGCC | 7 cut(s) | 110, 156, 308, 388, 556, 705, 1229 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtgZI | GCGATG | 1 cut(s) | 606 |
| BtrI | CACGTC | 1 cut(s) | 1005 |
| BtsCI | GGATG | 4 cut(s) | 51, 624, 964, 1293 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 410 |
| BveI | ACCTGC | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 9 cut(s) | 36, 48, 183, 332, 589, 729, 845, 852, 892 |
| CaiI | CAGNNNCTG | 1 cut(s) | 850 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 6 cut(s) | 231, 670, 883, 892, 894, 1064 |
| Cfr10I | RCCGGY | 3 cut(s) | 368, 587, 988 |
| Cfr13I | GGNCC | 5 cut(s) | 386, 440, 704, 1134, 1227 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| CseI | GACGC | 2 cut(s) | 307, 409 |
| Csp6I | GTAC | 3 cut(s) | 175, 395, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 6 cut(s) | 271, 802, 899, 1057, 1131, 1147 |
| CviQI | GTAC | 3 cut(s) | 175, 395, 407 |
| DdeI | CTNAG | 5 cut(s) | 423, 675, 1027, 1041, 1164 |
| DinI | GGCGCC | 2 cut(s) | 669, 1063 |
| DpnI | GATC | 6 cut(s) | 162, 195, 644, 789, 951, 1074 |
| DpnII | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 692 |
| EarI | CTCTTC | 2 cut(s) | 24, 692 |
| EciI | GGCGGA | 5 cut(s) | 206, 525, 574, 792, 1286 |
| Eco130I | CCWWGG | 2 cut(s) | 545, 953 |
| Eco47I | GGWCC | 2 cut(s) | 440, 1134 |
| Eco57I | CTGAAG | 3 cut(s) | 48, 264, 546 |
| Eco81I | CCTNAGG | 1 cut(s) | 1164 |
| Eco91I | GGTNACC | 1 cut(s) | 820 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1227 |
| EcoO65I | GGTNACC | 1 cut(s) | 820 |
| EcoRII | CCWGG | 5 cut(s) | 347, 382, 700, 1150, 1299 |
| EcoT14I | CCWWGG | 2 cut(s) | 545, 953 |
| EgeI | GGCGCC | 2 cut(s) | 669, 1063 |
| EheI | GGCGCC | 2 cut(s) | 669, 1063 |
| ErhI | CCWWGG | 2 cut(s) | 545, 953 |
| FaeI | CATG | 6 cut(s) | 274, 805, 902, 1060, 1134, 1150 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FatI | CATG | 6 cut(s) | 270, 801, 898, 1056, 1130, 1146 |
| FauI | CCCGC | 5 cut(s) | 55, 79, 498, 558, 643 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 7 cut(s) | 121, 139, 142, 717, 848, 895, 1119 |
| FokI | GGATG | 4 cut(s) | 38, 631, 971, 1280 |
| Fsp4HI | GCNGC | 7 cut(s) | 121, 139, 142, 717, 848, 895, 1119 |
| FspBI | CTAG | 3 cut(s) | 182, 513, 954 |
| GlaI | GCGC | 6 cut(s) | 230, 669, 882, 891, 893, 1063 |
| GluI | GCNGC | 7 cut(s) | 121, 139, 142, 717, 848, 895, 1119 |
| GsaI | CCCAGC | 1 cut(s) | 323 |
| GsuI | CTGGAG | 2 cut(s) | 323, 650 |
| HaeII | RGCGCY | 2 cut(s) | 671, 1065 |
| HaeIII | GGCC | 7 cut(s) | 110, 156, 308, 388, 556, 705, 1229 |
| HgaI | GACGC | 2 cut(s) | 307, 409 |
| HhaI | GCGC | 6 cut(s) | 231, 670, 883, 892, 894, 1064 |
| Hin1I | GRCGYC | 2 cut(s) | 668, 1062 |
| Hin1II | CATG | 6 cut(s) | 274, 805, 902, 1060, 1134, 1150 |
| Hin6I | GCGC | 6 cut(s) | 229, 668, 881, 890, 892, 1062 |
| HinP1I | GCGC | 6 cut(s) | 229, 668, 881, 890, 892, 1062 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 4 cut(s) | 482, 581, 679, 1290 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HphI | GGTGA | 2 cut(s) | 703, 814 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 359, 799 |
| Hpy188I | TCNGA | 8 cut(s) | 67, 508, 678, 737, 787, 909, 1030, 1259 |
| Hpy188III | TCNNGA | 5 cut(s) | 271, 696, 814, 998, 1076 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 359, 799 |
| HpyAV | CCTTC | 7 cut(s) | 521, 718, 802, 811, 820, 976, 1210 |
| HpyCH4III | ACNGT | 4 cut(s) | 11, 877, 913, 1051 |
| HpyCH4IV | ACGT | 6 cut(s) | 217, 277, 340, 1004, 1080, 1108 |
| HpyCH4V | TGCA | 6 cut(s) | 596, 622, 719, 727, 1023, 1130 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 320, 562, 584, 593, 887, 889, 1042, 1127 |
| HpyF3I | CTNAG | 5 cut(s) | 423, 675, 1027, 1041, 1164 |
| HpySE526I | ACGT | 6 cut(s) | 217, 277, 340, 1004, 1080, 1108 |
| Hsp92I | GRCGYC | 2 cut(s) | 668, 1062 |
| Hsp92II | CATG | 6 cut(s) | 274, 805, 902, 1060, 1134, 1150 |
| HspAI | GCGC | 6 cut(s) | 229, 668, 881, 890, 892, 1062 |
| KasI | GGCGCC | 2 cut(s) | 667, 1061 |
| Kpn2I | TCCGGA | 1 cut(s) | 997 |
| KpnI | GGTACC | 1 cut(s) | 410 |
| KroI | GCCGGC | 1 cut(s) | 587 |
| KroNI | GCCGGC | 1 cut(s) | 589 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| LguI | GCTCTTC | 2 cut(s) | 24, 692 |
| LmnI | GCTCC | 3 cut(s) | 226, 631, 985 |
| Lsp1109I | GCAGC | 4 cut(s) | 150, 153, 703, 859 |
| LweI | GCATC | 4 cut(s) | 583, 609, 752, 1032 |
| MaeI | CTAG | 3 cut(s) | 182, 513, 954 |
| MaeII | ACGT | 6 cut(s) | 217, 277, 340, 1004, 1080, 1108 |
| MaeIII | GTNAC | 6 cut(s) | 166, 336, 378, 558, 820, 917 |
| MalI | GATC | 6 cut(s) | 162, 195, 644, 789, 951, 1074 |
| MbiI | CCGCTC | 3 cut(s) | 504, 685, 1045 |
| MboI | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| MboII | GAAGA | 4 cut(s) | 41, 188, 307, 679 |
| MflI | RGATCY | 4 cut(s) | 160, 193, 949, 1072 |
| MhlI | GDGCHC | 2 cut(s) | 94, 1038 |
| MluCI | AATT | 2 cut(s) | 903, 1100 |
| Mly113I | GGCGCC | 2 cut(s) | 668, 1062 |
| MmeI | TCCRAC | 2 cut(s) | 90, 591 |
| MreI | CGCCGGCG | 1 cut(s) | 587 |
| MroI | TCCGGA | 1 cut(s) | 997 |
| MroNI | GCCGGC | 1 cut(s) | 587 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 859 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 716, 850 |
| MvaI | CCWGG | 5 cut(s) | 349, 384, 702, 1152, 1301 |
| MvnI | CGCG | 4 cut(s) | 74, 883, 892, 1142 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 320, 562, 584, 593, 887, 889, 1042, 1127 |
| NaeI | GCCGGC | 1 cut(s) | 589 |
| NarI | GGCGCC | 2 cut(s) | 668, 1062 |
| NciI | CCSGG | 6 cut(s) | 107, 117, 363, 647, 665, 1250 |
| NdeII | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| NgoMIV | GCCGGC | 1 cut(s) | 587 |
| NheI | GCTAGC | 1 cut(s) | 181 |
| NlaIII | CATG | 6 cut(s) | 274, 805, 902, 1060, 1134, 1150 |
| NlaIV | GGNNCC | 9 cut(s) | 367, 408, 441, 669, 706, 927, 951, 987, 1063 |
| NmuCI | GTSAC | 4 cut(s) | 166, 336, 558, 820 |
| NspI | RCATGY | 1 cut(s) | 1150 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PauI | GCGCGC | 1 cut(s) | 890 |
| PciSI | GCTCTTC | 2 cut(s) | 24, 692 |
| PdiI | GCCGGC | 1 cut(s) | 589 |
| PfeI | GAWTC | 4 cut(s) | 482, 581, 679, 1290 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 610, 850 |
| PkrI | GCNGC | 7 cut(s) | 122, 140, 143, 718, 849, 896, 1120 |
| PluTI | GGCGCC | 2 cut(s) | 671, 1065 |
| Ppu21I | YACGTR | 2 cut(s) | 341, 1109 |
| Psp6I | CCWGG | 5 cut(s) | 347, 382, 700, 1150, 1299 |
| PspEI | GGTNACC | 1 cut(s) | 820 |
| PspFI | CCCAGC | 1 cut(s) | 319 |
| PspGI | CCWGG | 5 cut(s) | 347, 382, 700, 1150, 1299 |
| PspN4I | GGNNCC | 9 cut(s) | 367, 408, 441, 669, 706, 927, 951, 987, 1063 |
| PspPI | GGNCC | 5 cut(s) | 386, 440, 704, 1134, 1227 |
| PstI | CTGCAG | 1 cut(s) | 729 |
| PstNI | CAGNNNCTG | 1 cut(s) | 850 |
| PsuI | RGATCY | 4 cut(s) | 160, 193, 949, 1072 |
| PteI | GCGCGC | 1 cut(s) | 890 |
| PvuII | CAGCTG | 1 cut(s) | 850 |
| RsaI | GTAC | 3 cut(s) | 176, 396, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 395, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 859 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 2 cut(s) | 24, 692 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 7 cut(s) | 121, 139, 142, 717, 848, 895, 1119 |
| Sau3AI | GATC | 6 cut(s) | 160, 193, 642, 787, 949, 1072 |
| Sau96I | GGNCC | 5 cut(s) | 386, 440, 704, 1134, 1227 |
| SbfI | CCTGCAGG | 1 cut(s) | 729 |
| SdaI | CCTGCAGG | 1 cut(s) | 729 |
| SduI | GDGCHC | 2 cut(s) | 94, 1038 |
| SfaNI | GCATC | 4 cut(s) | 583, 609, 752, 1032 |
| SfcI | CTRYAG | 3 cut(s) | 725, 876, 1277 |
| SfoI | GGCGCC | 2 cut(s) | 669, 1063 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 2 cut(s) | 440, 1134 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 859 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 729 |
| Sse9I | AATT | 2 cut(s) | 903, 1100 |
| SspDI | GGCGCC | 2 cut(s) | 667, 1061 |
| SspMI | CTAG | 3 cut(s) | 182, 513, 954 |
| StyI | CCWWGG | 2 cut(s) | 545, 953 |
| TaaI | ACNGT | 4 cut(s) | 11, 877, 913, 1051 |
| TaiI | ACGT | 6 cut(s) | 220, 280, 343, 1007, 1083, 1111 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TasI | AATT | 2 cut(s) | 903, 1100 |
| TauI | GCSGC | 3 cut(s) | 123, 897, 1121 |
| TfiI | GAWTC | 4 cut(s) | 482, 581, 679, 1290 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 192, 417 |
| TseFI | GTSAC | 4 cut(s) | 166, 336, 558, 820 |
| TseI | GCWGC | 4 cut(s) | 138, 141, 716, 847 |
| Tsp45I | GTSAC | 4 cut(s) | 166, 336, 558, 820 |
| TspDTI | ATGAA | 3 cut(s) | 188, 915, 1282 |
| TspRI | CASTG | 2 cut(s) | 192, 417 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 610, 850 |
| VpaK11BI | GGWCC | 2 cut(s) | 440, 1134 |
| XapI | RAATTY | 1 cut(s) | 903 |
| XceI | RCATGY | 1 cut(s) | 1150 |
| XmaJI | CCTAGG | 1 cut(s) | 953 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XspI | CTAG | 3 cut(s) | 182, 513, 954 |