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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 430 |
| Acc36I | ACCTGC | 2 cut(s) | 430, 577 |
| Acc65I | GGTACC | 2 cut(s) | 562, 589 |
| AccB1I | GGYRCC | 3 cut(s) | 562, 589, 842 |
| AccII | CGCG | 1 cut(s) | 523 |
| AclWI | GGATC | 2 cut(s) | 898, 911 |
| AcoI | YGGCCR | 1 cut(s) | 444 |
| AcsI | RAATTY | 2 cut(s) | 61, 992 |
| AcyI | GRCGYC | 2 cut(s) | 843, 938 |
| AfaI | GTAC | 3 cut(s) | 127, 564, 591 |
| AfeI | AGCGCT | 1 cut(s) | 852 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 193, 334, 439, 551, 639, 732, 733, 835 |
| AgsI | TTSAA | 2 cut(s) | 598, 988 |
| AjnI | CCWGG | 5 cut(s) | 638, 738, 756, 883, 899 |
| AluBI | AGCT | 6 cut(s) | 93, 353, 364, 400, 472, 610 |
| AluI | AGCT | 6 cut(s) | 93, 353, 364, 400, 472, 610 |
| Alw21I | GWGCWC | 1 cut(s) | 402 |
| Alw26I | GTCTC | 2 cut(s) | 115, 137 |
| AlwI | GGATC | 2 cut(s) | 898, 911 |
| Ama87I | CYCGRG | 2 cut(s) | 140, 401 |
| Aor51HI | AGCGCT | 1 cut(s) | 852 |
| AoxI | GGCC | 6 cut(s) | 429, 444, 495, 622, 741, 764 |
| ApaI | GGGCCC | 2 cut(s) | 626, 768 |
| ApeKI | GCWGC | 3 cut(s) | 197, 324, 350 |
| ApoI | RAATTY | 2 cut(s) | 61, 992 |
| Asp718I | GGTACC | 2 cut(s) | 562, 589 |
| AspLEI | GCGC | 7 cut(s) | 36, 235, 414, 484, 845, 853, 928 |
| AsuC2I | CCSGG | 2 cut(s) | 830, 923 |
| AsuHPI | GGTGA | 4 cut(s) | 232, 886, 988, 1010 |
| AvaI | CYCGRG | 2 cut(s) | 140, 401 |
| AvaII | GGWCC | 5 cut(s) | 283, 343, 376, 736, 887 |
| BaeGI | GKGCMC | 2 cut(s) | 626, 768 |
| BalI | TGGCCA | 1 cut(s) | 446 |
| BamHI | GGATCC | 1 cut(s) | 903 |
| BanI | GGYRCC | 3 cut(s) | 562, 589, 842 |
| BanII | GRGCYC | 3 cut(s) | 402, 626, 768 |
| BbsI | GAAGAC | 1 cut(s) | 230 |
| Bbv12I | GWGCWC | 1 cut(s) | 402 |
| BbvCI | CCTCAGC | 1 cut(s) | 426 |
| BbvI | GCAGC | 3 cut(s) | 209, 336, 362 |
| BccI | CCATC | 5 cut(s) | 107, 139, 448, 613, 923 |
| BceAI | ACGGC | 1 cut(s) | 243 |
| BciT130I | CCWGG | 5 cut(s) | 640, 740, 758, 885, 901 |
| BciVI | GTATCC | 1 cut(s) | 166 |
| BcnI | CCSGG | 2 cut(s) | 830, 923 |
| BcoDI | GTCTC | 2 cut(s) | 115, 137 |
| BfaI | CTAG | 5 cut(s) | 236, 531, 702, 794, 812 |
| BfoI | RGCGCY | 4 cut(s) | 236, 485, 846, 854 |
| BfuAI | ACCTGC | 2 cut(s) | 430, 577 |
| BfuI | GTATCC | 1 cut(s) | 166 |
| BglII | AGATCT | 1 cut(s) | 211 |
| BisI | GCNGC | 6 cut(s) | 198, 231, 325, 328, 351, 961 |
| BlpI | GCTNAGC | 1 cut(s) | 312 |
| BlsI | GCNGC | 6 cut(s) | 199, 232, 326, 329, 352, 962 |
| Bme1390I | CCNGG | 7 cut(s) | 640, 740, 758, 830, 885, 901, 923 |
| Bme18I | GGWCC | 5 cut(s) | 283, 343, 376, 736, 887 |
| BmeT110I | CYCGRG | 2 cut(s) | 140, 401 |
| BmiI | GGNNCC | 9 cut(s) | 285, 564, 591, 624, 737, 752, 766, 844, 905 |
| BmrFI | CCNGG | 7 cut(s) | 640, 740, 758, 830, 885, 901, 923 |
| BmsI | GCATC | 3 cut(s) | 162, 573, 792 |
| BpiI | GAAGAC | 1 cut(s) | 230 |
| BpmI | CTGGAG | 3 cut(s) | 95, 740, 883 |
| Bpu10I | CCTNAGC | 1 cut(s) | 426 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 312 |
| BpuEI | CTTGAG | 1 cut(s) | 223 |
| BpuMI | CCSGG | 2 cut(s) | 830, 923 |
| BsaHI | GRCGYC | 2 cut(s) | 843, 938 |
| BsaI | GGTCTC | 1 cut(s) | 137 |
| BsaJI | CCNNGG | 1 cut(s) | 638 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 193, 334, 439, 551, 639, 732, 733, 835 |
| Bse1I | ACTGG | 3 cut(s) | 339, 375, 771 |
| BseBI | CCWGG | 5 cut(s) | 640, 740, 758, 885, 901 |
| BseDI | CCNNGG | 1 cut(s) | 638 |
| BseGI | GGATG | 3 cut(s) | 624, 807, 915 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 193, 334, 439, 551, 639, 732, 733, 835 |
| BseMII | CTCAG | 3 cut(s) | 326, 417, 699 |
| BseNI | ACTGG | 3 cut(s) | 339, 375, 771 |
| BseRI | GAGGAG | 2 cut(s) | 92, 220 |
| BseSI | GKGCMC | 2 cut(s) | 626, 768 |
| BseXI | GCAGC | 3 cut(s) | 209, 336, 362 |
| BseYI | CCCAGC | 2 cut(s) | 187, 847 |
| BsgI | GTGCAG | 1 cut(s) | 359 |
| Bsh1236I | CGCG | 1 cut(s) | 523 |
| BshFI | GGCC | 6 cut(s) | 431, 446, 497, 624, 743, 766 |
| BshNI | GGYRCC | 3 cut(s) | 562, 589, 842 |
| BsiHKAI | GWGCWC | 1 cut(s) | 402 |
| BsiHKCI | CYCGRG | 2 cut(s) | 140, 401 |
| BsiSI | CCGG | 2 cut(s) | 829, 923 |
| BslFI | GGGAC | 2 cut(s) | 269, 749 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 193, 334, 439, 551, 639, 732, 733, 835 |
| BsmAI | GTCTC | 2 cut(s) | 115, 137 |
| BsmBI | CGTCTC | 1 cut(s) | 115 |
| BsmFI | GGGAC | 2 cut(s) | 269, 749 |
| BsnI | GGCC | 6 cut(s) | 431, 446, 497, 624, 743, 766 |
| Bso31I | GGTCTC | 1 cut(s) | 137 |
| BsoBI | CYCGRG | 2 cut(s) | 140, 401 |
| Bsp120I | GGGCCC | 2 cut(s) | 622, 764 |
| Bsp1286I | GDGCHC | 3 cut(s) | 402, 626, 768 |
| Bsp143I | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 312 |
| BspANI | GGCC | 6 cut(s) | 431, 446, 497, 624, 743, 766 |
| BspCNI | CTCAG | 3 cut(s) | 325, 418, 700 |
| BspFNI | CGCG | 1 cut(s) | 523 |
| BspLI | GGNNCC | 9 cut(s) | 285, 564, 591, 624, 737, 752, 766, 844, 905 |
| BspMI | ACCTGC | 2 cut(s) | 430, 577 |
| BspPI | GGATC | 2 cut(s) | 898, 911 |
| BspT107I | GGYRCC | 3 cut(s) | 562, 589, 842 |
| BspTNI | GGTCTC | 1 cut(s) | 137 |
| BsrI | ACTGG | 3 cut(s) | 339, 375, 771 |
| BssECI | CCNNGG | 1 cut(s) | 638 |
| BssMI | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| BssNI | GRCGYC | 2 cut(s) | 843, 938 |
| Bst2UI | CCWGG | 5 cut(s) | 640, 740, 758, 885, 901 |
| Bst4CI | ACNGT | 1 cut(s) | 539 |
| BstACI | GRCGYC | 2 cut(s) | 843, 938 |
| BstC8I | GCNNGC | 5 cut(s) | 366, 515, 841, 912, 930 |
| BstDEI | CTNAG | 3 cut(s) | 312, 426, 708 |
| BstF5I | GGATG | 3 cut(s) | 624, 807, 915 |
| BstFNI | CGCG | 1 cut(s) | 523 |
| BstH2I | RGCGCY | 4 cut(s) | 236, 485, 846, 854 |
| BstHHI | GCGC | 7 cut(s) | 36, 235, 414, 484, 845, 853, 928 |
| BstKTI | GATC | 4 cut(s) | 214, 696, 906, 1018 |
| BstMAI | GTCTC | 2 cut(s) | 115, 137 |
| BstMBI | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 197, 488, 503, 541, 698 |
| BstNI | CCWGG | 5 cut(s) | 640, 740, 758, 885, 901 |
| BstNSI | RCATGY | 1 cut(s) | 914 |
| BstSCI | CCNGG | 7 cut(s) | 638, 738, 756, 828, 883, 899, 921 |
| BstSLI | GKGCMC | 2 cut(s) | 626, 768 |
| BstUI | CGCG | 1 cut(s) | 523 |
| BstV1I | GCAGC | 3 cut(s) | 209, 336, 362 |
| BstV2I | GAAGAC | 1 cut(s) | 230 |
| BstX2I | RGATCY | 2 cut(s) | 211, 903 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 454, 688 |
| BstYI | RGATCY | 2 cut(s) | 211, 903 |
| BsuI | GTATCC | 1 cut(s) | 166 |
| BsuRI | GGCC | 6 cut(s) | 431, 446, 497, 624, 743, 766 |
| BtsCI | GGATG | 3 cut(s) | 624, 807, 915 |
| BtsIMutI | CAGTG | 3 cut(s) | 332, 368, 778 |
| BveI | ACCTGC | 2 cut(s) | 430, 577 |
| Cac8I | GCNNGC | 5 cut(s) | 366, 515, 841, 912, 930 |
| CfoI | GCGC | 7 cut(s) | 36, 235, 414, 484, 845, 853, 928 |
| CseI | GACGC | 2 cut(s) | 785, 946 |
| Csp6I | GTAC | 3 cut(s) | 126, 563, 590 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 535, 570 |
| CviAII | CATG | 3 cut(s) | 104, 911, 964 |
| CviQI | GTAC | 3 cut(s) | 126, 563, 590 |
| DdeI | CTNAG | 3 cut(s) | 312, 426, 708 |
| DinI | GGCGCC | 1 cut(s) | 844 |
| DpnI | GATC | 4 cut(s) | 213, 695, 905, 1017 |
| DpnII | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| EaeI | YGGCCR | 1 cut(s) | 444 |
| EciI | GGCGGA | 4 cut(s) | 389, 677, 848, 908 |
| Ecl136II | GAGCTC | 1 cut(s) | 400 |
| Eco24I | GRGCYC | 3 cut(s) | 402, 626, 768 |
| Eco31I | GGTCTC | 1 cut(s) | 137 |
| Eco32I | GATATC | 1 cut(s) | 184 |
| Eco47I | GGWCC | 5 cut(s) | 283, 343, 376, 736, 887 |
| Eco47III | AGCGCT | 1 cut(s) | 852 |
| Eco53kI | GAGCTC | 1 cut(s) | 400 |
| Eco88I | CYCGRG | 2 cut(s) | 140, 401 |
| EcoICRI | GAGCTC | 1 cut(s) | 400 |
| EcoO109I | RGGNCCY | 3 cut(s) | 343, 736, 764 |
| EcoRI | GAATTC | 1 cut(s) | 61 |
| EcoRII | CCWGG | 5 cut(s) | 638, 738, 756, 883, 899 |
| EcoRV | GATATC | 1 cut(s) | 184 |
| EcoT38I | GRGCYC | 3 cut(s) | 402, 626, 768 |
| EgeI | GGCGCC | 1 cut(s) | 844 |
| EheI | GGCGCC | 1 cut(s) | 844 |
| Esp3I | CGTCTC | 1 cut(s) | 115 |
| FaeI | CATG | 3 cut(s) | 107, 914, 967 |
| FaiI | YATR | 9 cut(s) | 105, 159, 356, 360, 578, 912, 965, 975, 977 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 185, 217 |
| FaqI | GGGAC | 2 cut(s) | 269, 749 |
| FatI | CATG | 3 cut(s) | 103, 910, 963 |
| FauI | CCCGC | 3 cut(s) | 294, 642, 726 |
| FauNDI | CATATG | 1 cut(s) | 975 |
| Fnu4HI | GCNGC | 6 cut(s) | 198, 231, 325, 328, 351, 961 |
| FokI | GGATG | 3 cut(s) | 631, 814, 902 |
| FriOI | GRGCYC | 3 cut(s) | 402, 626, 768 |
| Fsp4HI | GCNGC | 6 cut(s) | 198, 231, 325, 328, 351, 961 |
| FspBI | CTAG | 5 cut(s) | 236, 531, 702, 794, 812 |
| GlaI | GCGC | 7 cut(s) | 35, 234, 413, 483, 844, 852, 927 |
| GluI | GCNGC | 6 cut(s) | 198, 231, 325, 328, 351, 961 |
| GsaI | CCCAGC | 2 cut(s) | 191, 851 |
| GsuI | CTGGAG | 3 cut(s) | 95, 740, 883 |
| HaeII | RGCGCY | 4 cut(s) | 236, 485, 846, 854 |
| HaeIII | GGCC | 6 cut(s) | 431, 446, 497, 624, 743, 766 |
| HapII | CCGG | 2 cut(s) | 829, 923 |
| HgaI | GACGC | 2 cut(s) | 785, 946 |
| HhaI | GCGC | 7 cut(s) | 36, 235, 414, 484, 845, 853, 928 |
| Hin1I | GRCGYC | 2 cut(s) | 843, 938 |
| Hin1II | CATG | 3 cut(s) | 107, 914, 967 |
| Hin6I | GCGC | 7 cut(s) | 34, 233, 412, 482, 843, 851, 926 |
| HinP1I | GCGC | 7 cut(s) | 34, 233, 412, 482, 843, 851, 926 |
| HinfI | GANTC | 1 cut(s) | 665 |
| HpaII | CCGG | 2 cut(s) | 829, 923 |
| HphI | GGTGA | 4 cut(s) | 232, 886, 988, 1010 |
| Hpy166II | GTNNAC | 1 cut(s) | 128 |
| Hpy188III | TCNNGA | 2 cut(s) | 142, 988 |
| Hpy8I | GTNNAC | 1 cut(s) | 128 |
| HpyAV | CCTTC | 3 cut(s) | 389, 472, 994 |
| HpyCH4III | ACNGT | 1 cut(s) | 539 |
| HpyCH4IV | ACGT | 1 cut(s) | 124 |
| HpyCH4V | TGCA | 6 cut(s) | 153, 340, 350, 586, 805, 910 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 197, 488, 503, 541, 698 |
| HpyF3I | CTNAG | 3 cut(s) | 312, 426, 708 |
| HpySE526I | ACGT | 1 cut(s) | 124 |
| Hsp92I | GRCGYC | 2 cut(s) | 843, 938 |
| Hsp92II | CATG | 3 cut(s) | 107, 914, 967 |
| HspAI | GCGC | 7 cut(s) | 34, 233, 412, 482, 843, 851, 926 |
| KasI | GGCGCC | 1 cut(s) | 842 |
| KpnI | GGTACC | 2 cut(s) | 566, 593 |
| Kzo9I | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| LmnI | GCTCC | 3 cut(s) | 397, 409, 652 |
| Lsp1109I | GCAGC | 3 cut(s) | 209, 336, 362 |
| LweI | GCATC | 3 cut(s) | 162, 573, 792 |
| MaeI | CTAG | 5 cut(s) | 236, 531, 702, 794, 812 |
| MaeII | ACGT | 1 cut(s) | 124 |
| MaeIII | GTNAC | 4 cut(s) | 16, 666, 773, 948 |
| MalI | GATC | 4 cut(s) | 213, 695, 905, 1017 |
| MboI | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| MboII | GAAGA | 1 cut(s) | 235 |
| MflI | RGATCY | 2 cut(s) | 211, 903 |
| MhlI | GDGCHC | 3 cut(s) | 402, 626, 768 |
| MlsI | TGGCCA | 1 cut(s) | 446 |
| MluCI | AATT | 2 cut(s) | 61, 992 |
| MluNI | TGGCCA | 1 cut(s) | 446 |
| Mly113I | GGCGCC | 1 cut(s) | 843 |
| MlyI | GAGTC | 1 cut(s) | 674 |
| MmeI | TCCRAC | 1 cut(s) | 630 |
| Mox20I | TGGCCA | 1 cut(s) | 446 |
| MscI | TGGCCA | 1 cut(s) | 446 |
| MslI | CAYNNNNRTG | 3 cut(s) | 108, 452, 581 |
| Msp20I | TGGCCA | 1 cut(s) | 446 |
| MspA1I | CMGCKG | 1 cut(s) | 960 |
| MspI | CCGG | 2 cut(s) | 829, 923 |
| MspR9I | CCNGG | 7 cut(s) | 640, 740, 758, 830, 885, 901, 923 |
| MvaI | CCWGG | 5 cut(s) | 640, 740, 758, 885, 901 |
| MvnI | CGCG | 1 cut(s) | 523 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 197, 488, 503, 541, 698 |
| NarI | GGCGCC | 1 cut(s) | 843 |
| NciI | CCSGG | 2 cut(s) | 830, 923 |
| NdeI | CATATG | 1 cut(s) | 975 |
| NdeII | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| NlaIII | CATG | 3 cut(s) | 107, 914, 967 |
| NlaIV | GGNNCC | 9 cut(s) | 285, 564, 591, 624, 737, 752, 766, 844, 905 |
| NmeAIII | GCCGAG | 1 cut(s) | 342 |
| NmuCI | GTSAC | 4 cut(s) | 16, 666, 773, 948 |
| NspI | RCATGY | 1 cut(s) | 914 |
| PaeI | GCATGC | 1 cut(s) | 914 |
| PaeR7I | CTCGAG | 2 cut(s) | 140, 401 |
| PaqCI | CACCTGC | 1 cut(s) | 430 |
| PfoI | TCCNGGA | 1 cut(s) | 899 |
| PkrI | GCNGC | 6 cut(s) | 199, 232, 326, 329, 352, 962 |
| PleI | GAGTC | 1 cut(s) | 673 |
| PluTI | GGCGCC | 1 cut(s) | 846 |
| PpsI | GAGTC | 1 cut(s) | 673 |
| PpuMI | RGGWCCY | 2 cut(s) | 343, 736 |
| Psp124BI | GAGCTC | 1 cut(s) | 402 |
| Psp5II | RGGWCCY | 2 cut(s) | 343, 736 |
| Psp6I | CCWGG | 5 cut(s) | 638, 738, 756, 883, 899 |
| PspFI | CCCAGC | 2 cut(s) | 187, 847 |
| PspGI | CCWGG | 5 cut(s) | 638, 738, 756, 883, 899 |
| PspN4I | GGNNCC | 9 cut(s) | 285, 564, 591, 624, 737, 752, 766, 844, 905 |
| PspOMI | GGGCCC | 2 cut(s) | 622, 764 |
| PspPPI | RGGWCCY | 2 cut(s) | 343, 736 |
| PspXI | VCTCGAGB | 1 cut(s) | 401 |
| PsuI | RGATCY | 2 cut(s) | 211, 903 |
| RsaI | GTAC | 3 cut(s) | 127, 564, 591 |
| RsaNI | GTAC | 3 cut(s) | 126, 563, 590 |
| RseI | CAYNNNNRTG | 3 cut(s) | 108, 452, 581 |
| SacI | GAGCTC | 1 cut(s) | 402 |
| SatI | GCNGC | 6 cut(s) | 198, 231, 325, 328, 351, 961 |
| Sau3AI | GATC | 4 cut(s) | 211, 693, 903, 1015 |
| SchI | GAGTC | 1 cut(s) | 674 |
| ScrFI | CCNGG | 7 cut(s) | 640, 740, 758, 830, 885, 901, 923 |
| SduI | GDGCHC | 3 cut(s) | 402, 626, 768 |
| SfaNI | GCATC | 3 cut(s) | 162, 573, 792 |
| SfoI | GGCGCC | 1 cut(s) | 844 |
| Sfr274I | CTCGAG | 2 cut(s) | 140, 401 |
| SinI | GGWCC | 5 cut(s) | 283, 343, 376, 736, 887 |
| SlaI | CTCGAG | 2 cut(s) | 140, 401 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 108, 452, 581 |
| SmlI | CTYRAG | 3 cut(s) | 140, 202, 401 |
| SmoI | CTYRAG | 3 cut(s) | 140, 202, 401 |
| SphI | GCATGC | 1 cut(s) | 914 |
| Sse9I | AATT | 2 cut(s) | 61, 992 |
| SspDI | GGCGCC | 1 cut(s) | 842 |
| SspMI | CTAG | 5 cut(s) | 236, 531, 702, 794, 812 |
| SstI | GAGCTC | 1 cut(s) | 402 |
| StyD4I | CCNGG | 7 cut(s) | 638, 738, 756, 828, 883, 899, 921 |
| TaaI | ACNGT | 1 cut(s) | 539 |
| TaiI | ACGT | 1 cut(s) | 127 |
| TaqI | TCGA | 3 cut(s) | 141, 402, 1014 |
| TaqII | GACCGA | 1 cut(s) | 271 |
| TasI | AATT | 2 cut(s) | 61, 992 |
| TauI | GCSGC | 3 cut(s) | 233, 330, 963 |
| TscAI | CASTG | 3 cut(s) | 339, 375, 778 |
| TseFI | GTSAC | 4 cut(s) | 16, 666, 773, 948 |
| TseI | GCWGC | 3 cut(s) | 197, 324, 350 |
| Tsp45I | GTSAC | 4 cut(s) | 16, 666, 773, 948 |
| TspDTI | ATGAA | 1 cut(s) | 120 |
| TspRI | CASTG | 3 cut(s) | 339, 375, 778 |
| VpaK11BI | GGWCC | 5 cut(s) | 283, 343, 376, 736, 887 |
| XapI | RAATTY | 2 cut(s) | 61, 992 |
| XceI | RCATGY | 1 cut(s) | 914 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 110, 646, 736 |
| XhoI | CTCGAG | 2 cut(s) | 140, 401 |
| XspI | CTAG | 5 cut(s) | 236, 531, 702, 794, 812 |