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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 411 |
| AccII | CGCG | 1 cut(s) | 89 |
| AciI | CCGC | 8 cut(s) | 31, 89, 104, 168, 191, 352, 466, 528 |
| AclI | AACGTT | 1 cut(s) | 48 |
| AclWI | GGATC | 1 cut(s) | 168 |
| AcoI | YGGCCR | 1 cut(s) | 249 |
| AcsI | RAATTY | 1 cut(s) | 431 |
| AcuI | CTGAAG | 2 cut(s) | 41, 299 |
| AcyI | GRCGYC | 2 cut(s) | 412, 597 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 202, 329, 454 |
| AgsI | TTSAA | 2 cut(s) | 67, 565 |
| AjnI | CCWGG | 5 cut(s) | 82, 96, 133, 151, 468 |
| AluBI | AGCT | 1 cut(s) | 217 |
| AluI | AGCT | 1 cut(s) | 217 |
| Alw21I | GWGCWC | 1 cut(s) | 219 |
| AlwI | GGATC | 1 cut(s) | 168 |
| AoxI | GGCC | 6 cut(s) | 126, 234, 249, 333, 456, 481 |
| ApaI | GGGCCC | 1 cut(s) | 337 |
| ApeKI | GCWGC | 1 cut(s) | 374 |
| ApoI | RAATTY | 1 cut(s) | 431 |
| AspLEI | GCGC | 4 cut(s) | 89, 289, 414, 422 |
| AspS9I | GGNCC | 9 cut(s) | 33, 100, 127, 193, 235, 311, 333, 334, 456 |
| AsuHPI | GGTGA | 1 cut(s) | 455 |
| AvaII | GGWCC | 4 cut(s) | 33, 100, 193, 311 |
| BaeGI | GKGCMC | 1 cut(s) | 337 |
| BalI | TGGCCA | 1 cut(s) | 251 |
| BanI | GGYRCC | 1 cut(s) | 411 |
| BanII | GRGCYC | 2 cut(s) | 219, 337 |
| Bbv12I | GWGCWC | 1 cut(s) | 219 |
| BbvI | GCAGC | 1 cut(s) | 386 |
| BccI | CCATC | 1 cut(s) | 322 |
| BciT130I | CCWGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| BfaI | CTAG | 1 cut(s) | 326 |
| BfmI | CTRYAG | 1 cut(s) | 477 |
| BfoI | RGCGCY | 3 cut(s) | 290, 415, 423 |
| BisI | GCNGC | 3 cut(s) | 168, 375, 529 |
| BlsI | GCNGC | 3 cut(s) | 169, 376, 530 |
| Bme1390I | CCNGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| Bme18I | GGWCC | 4 cut(s) | 33, 100, 193, 311 |
| BmgT120I | GGNCC | 9 cut(s) | 33, 100, 127, 193, 235, 311, 333, 334, 456 |
| BmiI | GGNNCC | 6 cut(s) | 93, 102, 321, 334, 335, 413 |
| BmrFI | CCNGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| BmsI | GCATC | 1 cut(s) | 361 |
| BpmI | CTGGAG | 1 cut(s) | 311 |
| BsaHI | GRCGYC | 2 cut(s) | 412, 597 |
| BsaJI | CCNNGG | 1 cut(s) | 96 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 490, 520 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 202, 329, 454 |
| Bse3DI | GCAATG | 1 cut(s) | 648 |
| BseBI | CCWGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| BseDI | CCNNGG | 1 cut(s) | 96 |
| BseGI | GGATG | 1 cut(s) | 376 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 202, 329, 454 |
| BseMI | GCAATG | 1 cut(s) | 648 |
| BseMII | CTCAG | 2 cut(s) | 232, 499 |
| BseSI | GKGCMC | 1 cut(s) | 337 |
| BseXI | GCAGC | 1 cut(s) | 386 |
| BseYI | CCCAGC | 1 cut(s) | 416 |
| Bsh1236I | CGCG | 1 cut(s) | 89 |
| BshFI | GGCC | 6 cut(s) | 128, 236, 251, 335, 458, 483 |
| BshNI | GGYRCC | 1 cut(s) | 411 |
| BsiHKAI | GWGCWC | 1 cut(s) | 219 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 202, 329, 454 |
| BsnI | GGCC | 6 cut(s) | 128, 236, 251, 335, 458, 483 |
| Bsp120I | GGGCCC | 1 cut(s) | 333 |
| Bsp1286I | GDGCHC | 2 cut(s) | 219, 337 |
| Bsp143I | GATC | 2 cut(s) | 160, 450 |
| BspACI | CCGC | 8 cut(s) | 31, 89, 104, 168, 191, 352, 466, 528 |
| BspANI | GGCC | 6 cut(s) | 128, 236, 251, 335, 458, 483 |
| BspCNI | CTCAG | 2 cut(s) | 231, 498 |
| BspFNI | CGCG | 1 cut(s) | 89 |
| BspLI | GGNNCC | 6 cut(s) | 93, 102, 321, 334, 335, 413 |
| BspMAI | CTGCAG | 1 cut(s) | 481 |
| BspPI | GGATC | 1 cut(s) | 168 |
| BspQI | GCTCTTC | 1 cut(s) | 17 |
| BspT107I | GGYRCC | 1 cut(s) | 411 |
| BsrDI | GCAATG | 1 cut(s) | 648 |
| BssECI | CCNNGG | 1 cut(s) | 96 |
| BssMI | GATC | 2 cut(s) | 160, 450 |
| BssNI | GRCGYC | 2 cut(s) | 412, 597 |
| Bst2UI | CCWGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| Bst4CI | ACNGT | 1 cut(s) | 76 |
| Bst6I | CTCTTC | 3 cut(s) | 17, 68, 275 |
| BstACI | GRCGYC | 2 cut(s) | 412, 597 |
| BstC8I | GCNNGC | 3 cut(s) | 29, 410, 481 |
| BstDEI | CTNAG | 2 cut(s) | 218, 485 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 200, 327 |
| BstF5I | GGATG | 1 cut(s) | 376 |
| BstFNI | CGCG | 1 cut(s) | 89 |
| BstH2I | RGCGCY | 3 cut(s) | 290, 415, 423 |
| BstHHI | GCGC | 4 cut(s) | 89, 289, 414, 422 |
| BstKTI | GATC | 2 cut(s) | 163, 453 |
| BstMBI | GATC | 2 cut(s) | 160, 450 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 144, 293 |
| BstNI | CCWGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| BstSCI | CCNGG | 5 cut(s) | 82, 96, 133, 151, 468 |
| BstSFI | CTRYAG | 1 cut(s) | 477 |
| BstSLI | GKGCMC | 1 cut(s) | 337 |
| BstUI | CGCG | 1 cut(s) | 89 |
| BstV1I | GCAGC | 1 cut(s) | 386 |
| BstX2I | RGATCY | 1 cut(s) | 160 |
| BstYI | RGATCY | 1 cut(s) | 160 |
| BsuRI | GGCC | 6 cut(s) | 128, 236, 251, 335, 458, 483 |
| BtsCI | GGATG | 1 cut(s) | 376 |
| BtsIMutI | CAGTG | 1 cut(s) | 625 |
| Cac8I | GCNNGC | 3 cut(s) | 29, 410, 481 |
| CfoI | GCGC | 4 cut(s) | 89, 289, 414, 422 |
| Cfr13I | GGNCC | 9 cut(s) | 33, 100, 127, 193, 235, 311, 333, 334, 456 |
| CseI | GACGC | 1 cut(s) | 605 |
| CviAII | CATG | 1 cut(s) | 532 |
| CviJI | RGCY | 8 cut(s) | 128, 217, 236, 251, 335, 392, 458, 483 |
| CviKI_1 | RGCY | 8 cut(s) | 128, 217, 236, 251, 335, 392, 458, 483 |
| DdeI | CTNAG | 2 cut(s) | 218, 485 |
| DinI | GGCGCC | 1 cut(s) | 413 |
| DpnI | GATC | 2 cut(s) | 162, 452 |
| DpnII | GATC | 2 cut(s) | 160, 450 |
| EaeI | YGGCCR | 1 cut(s) | 249 |
| Eam1104I | CTCTTC | 3 cut(s) | 17, 68, 275 |
| EarI | CTCTTC | 3 cut(s) | 17, 68, 275 |
| EciI | GGCGGA | 2 cut(s) | 46, 206 |
| Ecl136II | GAGCTC | 1 cut(s) | 217 |
| Eco147I | AGGCCT | 1 cut(s) | 483 |
| Eco24I | GRGCYC | 2 cut(s) | 219, 337 |
| Eco47I | GGWCC | 4 cut(s) | 33, 100, 193, 311 |
| Eco53kI | GAGCTC | 1 cut(s) | 217 |
| Eco57I | CTGAAG | 2 cut(s) | 41, 299 |
| EcoICRI | GAGCTC | 1 cut(s) | 217 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 200, 327 |
| EcoO109I | RGGNCCY | 2 cut(s) | 100, 333 |
| EcoRII | CCWGG | 5 cut(s) | 82, 96, 133, 151, 468 |
| EcoT38I | GRGCYC | 2 cut(s) | 219, 337 |
| EgeI | GGCGCC | 1 cut(s) | 413 |
| EheI | GGCGCC | 1 cut(s) | 413 |
| FaeI | CATG | 1 cut(s) | 535 |
| FaiI | YATR | 8 cut(s) | 173, 175, 177, 444, 498, 533, 552, 554 |
| FatI | CATG | 1 cut(s) | 531 |
| FauI | CCCGC | 1 cut(s) | 111 |
| FauNDI | CATATG | 1 cut(s) | 552 |
| Fnu4HI | GCNGC | 3 cut(s) | 168, 375, 529 |
| FokI | GGATG | 1 cut(s) | 383 |
| FriOI | GRGCYC | 2 cut(s) | 219, 337 |
| Fsp4HI | GCNGC | 3 cut(s) | 168, 375, 529 |
| FspBI | CTAG | 1 cut(s) | 326 |
| GlaI | GCGC | 4 cut(s) | 88, 288, 413, 421 |
| GluI | GCNGC | 3 cut(s) | 168, 375, 529 |
| GsaI | CCCAGC | 1 cut(s) | 420 |
| GsuI | CTGGAG | 1 cut(s) | 311 |
| HaeII | RGCGCY | 3 cut(s) | 290, 415, 423 |
| HaeIII | GGCC | 6 cut(s) | 128, 236, 251, 335, 458, 483 |
| HgaI | GACGC | 1 cut(s) | 605 |
| HhaI | GCGC | 4 cut(s) | 89, 289, 414, 422 |
| Hin1I | GRCGYC | 2 cut(s) | 412, 597 |
| Hin1II | CATG | 1 cut(s) | 535 |
| Hin6I | GCGC | 4 cut(s) | 87, 287, 412, 420 |
| HinP1I | GCGC | 4 cut(s) | 87, 287, 412, 420 |
| HincII | GTYRAC | 1 cut(s) | 52 |
| HindII | GTYRAC | 1 cut(s) | 52 |
| HinfI | GANTC | 4 cut(s) | 109, 267, 473, 505 |
| HpaI | GTTAAC | 1 cut(s) | 52 |
| HphI | GGTGA | 1 cut(s) | 455 |
| Hpy166II | GTNNAC | 1 cut(s) | 52 |
| Hpy188I | TCNGA | 4 cut(s) | 42, 221, 319, 488 |
| Hpy188III | TCNNGA | 2 cut(s) | 264, 448 |
| Hpy8I | GTNNAC | 1 cut(s) | 52 |
| Hpy99I | CGWCG | 2 cut(s) | 275, 342 |
| HpyAV | CCTTC | 2 cut(s) | 553, 571 |
| HpyCH4III | ACNGT | 1 cut(s) | 76 |
| HpyCH4IV | ACGT | 1 cut(s) | 48 |
| HpyCH4V | TGCA | 2 cut(s) | 374, 479 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 144, 293 |
| HpyF3I | CTNAG | 2 cut(s) | 218, 485 |
| HpySE526I | ACGT | 1 cut(s) | 48 |
| Hsp92I | GRCGYC | 2 cut(s) | 412, 597 |
| Hsp92II | CATG | 1 cut(s) | 535 |
| HspAI | GCGC | 4 cut(s) | 87, 287, 412, 420 |
| KasI | GGCGCC | 1 cut(s) | 411 |
| KspAI | GTTAAC | 1 cut(s) | 52 |
| Kzo9I | GATC | 2 cut(s) | 160, 450 |
| LguI | GCTCTTC | 1 cut(s) | 17 |
| LmnI | GCTCC | 1 cut(s) | 214 |
| Lsp1109I | GCAGC | 1 cut(s) | 386 |
| LweI | GCATC | 1 cut(s) | 361 |
| MaeI | CTAG | 1 cut(s) | 326 |
| MaeII | ACGT | 1 cut(s) | 48 |
| MaeIII | GTNAC | 1 cut(s) | 571 |
| MalI | GATC | 2 cut(s) | 162, 452 |
| MboI | GATC | 2 cut(s) | 160, 450 |
| MboII | GAAGA | 4 cut(s) | 34, 55, 292, 396 |
| MflI | RGATCY | 1 cut(s) | 160 |
| MhlI | GDGCHC | 2 cut(s) | 219, 337 |
| MlsI | TGGCCA | 1 cut(s) | 251 |
| MluCI | AATT | 2 cut(s) | 378, 431 |
| MluNI | TGGCCA | 1 cut(s) | 251 |
| Mly113I | GGCGCC | 1 cut(s) | 412 |
| MlyI | GAGTC | 2 cut(s) | 103, 499 |
| MmeI | TCCRAC | 1 cut(s) | 65 |
| Mox20I | TGGCCA | 1 cut(s) | 251 |
| MscI | TGGCCA | 1 cut(s) | 251 |
| MseI | TTAA | 2 cut(s) | 51, 357 |
| MslI | CAYNNNNRTG | 1 cut(s) | 257 |
| Msp20I | TGGCCA | 1 cut(s) | 251 |
| MspA1I | CMGCKG | 2 cut(s) | 106, 528 |
| MspR9I | CCNGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| MvaI | CCWGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| MvnI | CGCG | 1 cut(s) | 89 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 144, 293 |
| NarI | GGCGCC | 1 cut(s) | 412 |
| NdeI | CATATG | 1 cut(s) | 552 |
| NdeII | GATC | 2 cut(s) | 160, 450 |
| NlaIII | CATG | 1 cut(s) | 535 |
| NlaIV | GGNNCC | 6 cut(s) | 93, 102, 321, 334, 335, 413 |
| PceI | AGGCCT | 1 cut(s) | 483 |
| PciSI | GCTCTTC | 1 cut(s) | 17 |
| PfeI | GAWTC | 2 cut(s) | 267, 473 |
| PkrI | GCNGC | 3 cut(s) | 169, 376, 530 |
| PleI | GAGTC | 2 cut(s) | 103, 499 |
| PluTI | GGCGCC | 1 cut(s) | 415 |
| PpsI | GAGTC | 2 cut(s) | 103, 499 |
| PpuMI | RGGWCCY | 1 cut(s) | 100 |
| Psp124BI | GAGCTC | 1 cut(s) | 219 |
| Psp1406I | AACGTT | 1 cut(s) | 48 |
| Psp5II | RGGWCCY | 1 cut(s) | 100 |
| Psp6I | CCWGG | 5 cut(s) | 82, 96, 133, 151, 468 |
| PspFI | CCCAGC | 1 cut(s) | 416 |
| PspGI | CCWGG | 5 cut(s) | 82, 96, 133, 151, 468 |
| PspN4I | GGNNCC | 6 cut(s) | 93, 102, 321, 334, 335, 413 |
| PspOMI | GGGCCC | 1 cut(s) | 333 |
| PspPI | GGNCC | 9 cut(s) | 33, 100, 127, 193, 235, 311, 333, 334, 456 |
| PspPPI | RGGWCCY | 1 cut(s) | 100 |
| PstI | CTGCAG | 1 cut(s) | 481 |
| PsuI | RGATCY | 1 cut(s) | 160 |
| RseI | CAYNNNNRTG | 1 cut(s) | 257 |
| SacI | GAGCTC | 1 cut(s) | 219 |
| SapI | GCTCTTC | 1 cut(s) | 17 |
| SaqAI | TTAA | 2 cut(s) | 51, 357 |
| SatI | GCNGC | 3 cut(s) | 168, 375, 529 |
| Sau3AI | GATC | 2 cut(s) | 160, 450 |
| Sau96I | GGNCC | 9 cut(s) | 33, 100, 127, 193, 235, 311, 333, 334, 456 |
| SchI | GAGTC | 2 cut(s) | 103, 499 |
| ScrFI | CCNGG | 5 cut(s) | 84, 98, 135, 153, 470 |
| SduI | GDGCHC | 2 cut(s) | 219, 337 |
| SetI | ASST | 6 cut(s) | 51, 198, 219, 306, 325, 563 |
| SfaNI | GCATC | 1 cut(s) | 361 |
| SfcI | CTRYAG | 1 cut(s) | 477 |
| SfoI | GGCGCC | 1 cut(s) | 413 |
| SinI | GGWCC | 4 cut(s) | 33, 100, 193, 311 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 257 |
| Sse9I | AATT | 2 cut(s) | 378, 431 |
| SseBI | AGGCCT | 1 cut(s) | 483 |
| SsiI | CCGC | 8 cut(s) | 31, 89, 104, 168, 191, 352, 466, 528 |
| SspDI | GGCGCC | 1 cut(s) | 411 |
| SspMI | CTAG | 1 cut(s) | 326 |
| SstI | GAGCTC | 1 cut(s) | 219 |
| StuI | AGGCCT | 1 cut(s) | 483 |
| StyD4I | CCNGG | 5 cut(s) | 82, 96, 133, 151, 468 |
| TaaI | ACNGT | 1 cut(s) | 76 |
| TaiI | ACGT | 1 cut(s) | 51 |
| TaqI | TCGA | 1 cut(s) | 270 |
| TasI | AATT | 2 cut(s) | 378, 431 |
| TauI | GCSGC | 2 cut(s) | 170, 531 |
| TfiI | GAWTC | 2 cut(s) | 267, 473 |
| Tru1I | TTAA | 2 cut(s) | 51, 357 |
| Tru9I | TTAA | 2 cut(s) | 51, 357 |
| TscAI | CASTG | 1 cut(s) | 625 |
| TseI | GCWGC | 1 cut(s) | 374 |
| TspDTI | ATGAA | 1 cut(s) | 539 |
| TspRI | CASTG | 1 cut(s) | 625 |
| VpaK11BI | GGWCC | 4 cut(s) | 33, 100, 193, 311 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 200, 327 |
| XapI | RAATTY | 1 cut(s) | 431 |
| XspI | CTAG | 1 cut(s) | 326 |