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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 199 |
| Acc65I | GGTACC | 1 cut(s) | 35 |
| AccB1I | GGYRCC | 1 cut(s) | 35 |
| AciI | CCGC | 6 cut(s) | 20, 63, 170, 253, 372, 419 |
| AcoI | YGGCCR | 2 cut(s) | 52, 416 |
| AcsI | RAATTY | 1 cut(s) | 206 |
| AcyI | GRCGYC | 1 cut(s) | 334 |
| AfaI | GTAC | 2 cut(s) | 37, 393 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 32, 50, 317 |
| AgsI | TTSAA | 1 cut(s) | 344 |
| AluBI | AGCT | 2 cut(s) | 113, 176 |
| AluI | AGCT | 2 cut(s) | 113, 176 |
| Ama87I | CYCGRG | 1 cut(s) | 351 |
| AoxI | GGCC | 5 cut(s) | 52, 249, 283, 369, 416 |
| ApeKI | GCWGC | 5 cut(s) | 65, 68, 95, 255, 305 |
| ApoI | RAATTY | 1 cut(s) | 206 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 33, 65 |
| Asp718I | GGTACC | 1 cut(s) | 35 |
| AspLEI | GCGC | 1 cut(s) | 275 |
| AspS9I | GGNCC | 3 cut(s) | 23, 250, 369 |
| AsuHPI | GGTGA | 1 cut(s) | 202 |
| AvaI | CYCGRG | 1 cut(s) | 351 |
| AvaII | GGWCC | 1 cut(s) | 23 |
| BanI | GGYRCC | 1 cut(s) | 35 |
| BbvI | GCAGC | 5 cut(s) | 52, 55, 107, 267, 317 |
| BccI | CCATC | 2 cut(s) | 26, 160 |
| BceAI | ACGGC | 1 cut(s) | 431 |
| BglII | AGATCT | 1 cut(s) | 88 |
| BisI | GCNGC | 7 cut(s) | 66, 69, 96, 256, 306, 372, 419 |
| BlsI | GCNGC | 7 cut(s) | 67, 70, 97, 257, 307, 373, 420 |
| Bme18I | GGWCC | 1 cut(s) | 23 |
| BmeT110I | CYCGRG | 1 cut(s) | 351 |
| BmgT120I | GGNCC | 3 cut(s) | 23, 250, 369 |
| BmiI | GGNNCC | 4 cut(s) | 15, 24, 25, 37 |
| BmsI | GCATC | 1 cut(s) | 14 |
| Bpu10I | CCTNAGC | 1 cut(s) | 287 |
| BsaHI | GRCGYC | 1 cut(s) | 334 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 32, 50, 317 |
| BseGI | GGATG | 1 cut(s) | 244 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 32, 50, 317 |
| BseX3I | CGGCCG | 2 cut(s) | 52, 416 |
| BseXI | GCAGC | 5 cut(s) | 52, 55, 107, 267, 317 |
| Bsh1285I | CGRYCG | 2 cut(s) | 55, 419 |
| BshFI | GGCC | 5 cut(s) | 54, 251, 285, 371, 418 |
| BshNI | GGYRCC | 1 cut(s) | 35 |
| BsiEI | CGRYCG | 2 cut(s) | 55, 419 |
| BsiHKCI | CYCGRG | 1 cut(s) | 351 |
| BsiSI | CCGG | 1 cut(s) | 248 |
| BslFI | GGGAC | 1 cut(s) | 36 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 32, 50, 317 |
| BsmFI | GGGAC | 1 cut(s) | 36 |
| BsnI | GGCC | 5 cut(s) | 54, 251, 285, 371, 418 |
| BsoBI | CYCGRG | 1 cut(s) | 351 |
| Bsp143I | GATC | 1 cut(s) | 88 |
| BspACI | CCGC | 6 cut(s) | 20, 63, 170, 253, 372, 419 |
| BspANI | GGCC | 5 cut(s) | 54, 251, 285, 371, 418 |
| BspLI | GGNNCC | 4 cut(s) | 15, 24, 25, 37 |
| BspT107I | GGYRCC | 1 cut(s) | 35 |
| BssMI | GATC | 1 cut(s) | 88 |
| BssNI | GRCGYC | 1 cut(s) | 334 |
| BstACI | GRCGYC | 1 cut(s) | 334 |
| BstC8I | GCNNGC | 2 cut(s) | 18, 253 |
| BstDEI | CTNAG | 1 cut(s) | 287 |
| BstF5I | GGATG | 1 cut(s) | 244 |
| BstHHI | GCGC | 1 cut(s) | 275 |
| BstKTI | GATC | 1 cut(s) | 91 |
| BstMBI | GATC | 1 cut(s) | 88 |
| BstMCI | CGRYCG | 2 cut(s) | 55, 419 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 74 |
| BstV1I | GCAGC | 5 cut(s) | 52, 55, 107, 267, 317 |
| BstX2I | RGATCY | 1 cut(s) | 88 |
| BstYI | RGATCY | 1 cut(s) | 88 |
| BstZI | CGGCCG | 2 cut(s) | 52, 416 |
| BsuRI | GGCC | 5 cut(s) | 54, 251, 285, 371, 418 |
| BtsCI | GGATG | 1 cut(s) | 244 |
| Cac8I | GCNNGC | 2 cut(s) | 18, 253 |
| CfoI | GCGC | 1 cut(s) | 275 |
| Cfr13I | GGNCC | 3 cut(s) | 23, 250, 369 |
| CseI | GACGC | 2 cut(s) | 202, 323 |
| Csp6I | GTAC | 2 cut(s) | 36, 392 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 337, 372 |
| CviAII | CATG | 1 cut(s) | 100 |
| CviQI | GTAC | 2 cut(s) | 36, 392 |
| DdeI | CTNAG | 1 cut(s) | 287 |
| DpnI | GATC | 1 cut(s) | 90 |
| DpnII | GATC | 1 cut(s) | 88 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 199 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 199 |
| EaeI | YGGCCR | 2 cut(s) | 52, 416 |
| EagI | CGGCCG | 2 cut(s) | 52, 416 |
| EciI | GGCGGA | 1 cut(s) | 185 |
| EclXI | CGGCCG | 2 cut(s) | 52, 416 |
| Eco47I | GGWCC | 1 cut(s) | 23 |
| Eco52I | CGGCCG | 2 cut(s) | 52, 416 |
| Eco88I | CYCGRG | 1 cut(s) | 351 |
| EcoO109I | RGGNCCY | 1 cut(s) | 23 |
| FaeI | CATG | 1 cut(s) | 103 |
| FaiI | YATR | 4 cut(s) | 101, 129, 293, 426 |
| FaqI | GGGAC | 1 cut(s) | 36 |
| FatI | CATG | 1 cut(s) | 99 |
| FauI | CCCGC | 2 cut(s) | 13, 260 |
| Fnu4HI | GCNGC | 7 cut(s) | 66, 69, 96, 256, 306, 372, 419 |
| FokI | GGATG | 1 cut(s) | 231 |
| Fsp4HI | GCNGC | 7 cut(s) | 66, 69, 96, 256, 306, 372, 419 |
| GlaI | GCGC | 1 cut(s) | 274 |
| GluI | GCNGC | 7 cut(s) | 66, 69, 96, 256, 306, 372, 419 |
| HaeIII | GGCC | 5 cut(s) | 54, 251, 285, 371, 418 |
| HapII | CCGG | 1 cut(s) | 248 |
| HgaI | GACGC | 2 cut(s) | 202, 323 |
| HhaI | GCGC | 1 cut(s) | 275 |
| Hin1I | GRCGYC | 1 cut(s) | 334 |
| Hin1II | CATG | 1 cut(s) | 103 |
| Hin6I | GCGC | 1 cut(s) | 273 |
| HinP1I | GCGC | 1 cut(s) | 273 |
| HinfI | GANTC | 1 cut(s) | 410 |
| HpaII | CCGG | 1 cut(s) | 248 |
| HphI | GGTGA | 1 cut(s) | 202 |
| Hpy99I | CGWCG | 2 cut(s) | 215, 339 |
| HpyCH4V | TGCA | 2 cut(s) | 95, 127 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 74 |
| HpyF3I | CTNAG | 1 cut(s) | 287 |
| Hsp92I | GRCGYC | 1 cut(s) | 334 |
| Hsp92II | CATG | 1 cut(s) | 103 |
| HspAI | GCGC | 1 cut(s) | 273 |
| KflI | GGGWCCC | 1 cut(s) | 23 |
| KpnI | GGTACC | 1 cut(s) | 39 |
| Kzo9I | GATC | 1 cut(s) | 88 |
| LmnI | GCTCC | 1 cut(s) | 13 |
| LpnPI | CCDG | 4 cut(s) | 30, 97, 261, 380 |
| Lsp1109I | GCAGC | 5 cut(s) | 52, 55, 107, 267, 317 |
| LweI | GCATC | 1 cut(s) | 14 |
| MaeIII | GTNAC | 2 cut(s) | 58, 190 |
| MalI | GATC | 1 cut(s) | 90 |
| MboI | GATC | 1 cut(s) | 88 |
| MfeI | CAATTG | 1 cut(s) | 386 |
| MflI | RGATCY | 1 cut(s) | 88 |
| MluCI | AATT | 4 cut(s) | 107, 206, 363, 386 |
| MlyI | GAGTC | 1 cut(s) | 404 |
| MmeI | TCCRAC | 1 cut(s) | 84 |
| MnlI | CCTC | 2 cut(s) | 390, 400 |
| MseI | TTAA | 1 cut(s) | 218 |
| MspA1I | CMGCKG | 1 cut(s) | 65 |
| MspI | CCGG | 1 cut(s) | 248 |
| MunI | CAATTG | 1 cut(s) | 386 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 74 |
| NdeII | GATC | 1 cut(s) | 88 |
| NlaIII | CATG | 1 cut(s) | 103 |
| NlaIV | GGNNCC | 4 cut(s) | 15, 24, 25, 37 |
| NmeAIII | GCCGAG | 2 cut(s) | 80, 261 |
| NmuCI | GTSAC | 1 cut(s) | 190 |
| PkrI | GCNGC | 7 cut(s) | 67, 70, 97, 257, 307, 373, 420 |
| PleI | GAGTC | 1 cut(s) | 404 |
| PpsI | GAGTC | 1 cut(s) | 404 |
| PpuMI | RGGWCCY | 1 cut(s) | 23 |
| Psp5II | RGGWCCY | 1 cut(s) | 23 |
| PspN4I | GGNNCC | 4 cut(s) | 15, 24, 25, 37 |
| PspPI | GGNCC | 3 cut(s) | 23, 250, 369 |
| PspPPI | RGGWCCY | 1 cut(s) | 23 |
| PsuI | RGATCY | 1 cut(s) | 88 |
| RsaI | GTAC | 2 cut(s) | 37, 393 |
| RsaNI | GTAC | 2 cut(s) | 36, 392 |
| SaqAI | TTAA | 1 cut(s) | 218 |
| SatI | GCNGC | 7 cut(s) | 66, 69, 96, 256, 306, 372, 419 |
| Sau3AI | GATC | 1 cut(s) | 88 |
| Sau96I | GGNCC | 3 cut(s) | 23, 250, 369 |
| SchI | GAGTC | 1 cut(s) | 404 |
| SetI | ASST | 4 cut(s) | 46, 115, 178, 382 |
| SfaNI | GCATC | 1 cut(s) | 14 |
| SinI | GGWCC | 1 cut(s) | 23 |
| Sse9I | AATT | 4 cut(s) | 107, 206, 363, 386 |
| SsiI | CCGC | 6 cut(s) | 20, 63, 170, 253, 372, 419 |
| TaqI | TCGA | 1 cut(s) | 210 |
| TasI | AATT | 4 cut(s) | 107, 206, 363, 386 |
| TauI | GCSGC | 2 cut(s) | 374, 421 |
| Tru1I | TTAA | 1 cut(s) | 218 |
| Tru9I | TTAA | 1 cut(s) | 218 |
| TseFI | GTSAC | 1 cut(s) | 190 |
| TseI | GCWGC | 5 cut(s) | 65, 68, 95, 255, 305 |
| Tsp45I | GTSAC | 1 cut(s) | 190 |
| VpaK11BI | GGWCC | 1 cut(s) | 23 |
| XapI | RAATTY | 1 cut(s) | 206 |