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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 4 cut(s) | 310, 517, 526, 619 |
| Acc36I | ACCTGC | 5 cut(s) | 310, 457, 517, 526, 619 |
| Acc65I | GGTACC | 2 cut(s) | 442, 469 |
| AccB1I | GGYRCC | 3 cut(s) | 442, 469, 732 |
| AccBSI | CCGCTC | 1 cut(s) | 576 |
| AccII | CGCG | 2 cut(s) | 282, 403 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 4 cut(s) | 48, 157, 788, 801 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AcuI | CTGAAG | 2 cut(s) | 374, 656 |
| AcyI | GRCGYC | 1 cut(s) | 733 |
| AfaI | GTAC | 4 cut(s) | 444, 459, 471, 819 |
| AgeI | ACCGGT | 2 cut(s) | 214, 659 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjiI | CACGTC | 1 cut(s) | 766 |
| AjnI | CCWGG | 5 cut(s) | 147, 527, 645, 768, 789 |
| AluBI | AGCT | 4 cut(s) | 244, 352, 628, 741 |
| AluI | AGCT | 4 cut(s) | 244, 352, 628, 741 |
| Alw21I | GWGCWC | 1 cut(s) | 410 |
| AlwI | GGATC | 4 cut(s) | 48, 157, 788, 801 |
| AoxI | GGCC | 6 cut(s) | 309, 324, 502, 630, 653, 771 |
| ApaI | GGGCCC | 1 cut(s) | 506 |
| ApeKI | GCWGC | 2 cut(s) | 201, 204 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AsiGI | ACCGGT | 2 cut(s) | 214, 659 |
| Asp718I | GGTACC | 2 cut(s) | 442, 469 |
| AspLEI | GCGC | 4 cut(s) | 282, 294, 364, 735 |
| AspS9I | GGNCC | 6 cut(s) | 256, 310, 502, 503, 631, 653 |
| AsuC2I | CCSGG | 3 cut(s) | 198, 720, 813 |
| AsuHPI | GGTGA | 2 cut(s) | 674, 776 |
| AvaII | GGWCC | 1 cut(s) | 256 |
| BaeGI | GKGCMC | 1 cut(s) | 506 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BamHI | GGATCC | 1 cut(s) | 793 |
| BanI | GGYRCC | 3 cut(s) | 442, 469, 732 |
| BanII | GRGCYC | 1 cut(s) | 506 |
| Bbv12I | GWGCWC | 1 cut(s) | 410 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 2 cut(s) | 213, 216 |
| BccI | CCATC | 3 cut(s) | 66, 493, 813 |
| BciT130I | CCWGG | 5 cut(s) | 149, 529, 647, 770, 791 |
| BcnI | CCSGG | 3 cut(s) | 198, 720, 813 |
| BfaI | CTAG | 2 cut(s) | 591, 702 |
| BfoI | RGCGCY | 2 cut(s) | 365, 736 |
| BfuAI | ACCTGC | 5 cut(s) | 310, 457, 517, 526, 619 |
| BisI | GCNGC | 4 cut(s) | 202, 205, 208, 851 |
| BlpI | GCTNAGC | 1 cut(s) | 192 |
| BlsI | GCNGC | 4 cut(s) | 203, 206, 209, 852 |
| Bme1390I | CCNGG | 8 cut(s) | 149, 198, 529, 647, 720, 770, 791, 813 |
| Bme18I | GGWCC | 1 cut(s) | 256 |
| BmgBI | CACGTC | 1 cut(s) | 766 |
| BmgT120I | GGNCC | 6 cut(s) | 256, 310, 502, 503, 631, 653 |
| BmiI | GGNNCC | 7 cut(s) | 144, 444, 471, 504, 654, 734, 795 |
| BmrFI | CCNGG | 8 cut(s) | 149, 198, 529, 647, 720, 770, 791, 813 |
| BmsI | GCATC | 3 cut(s) | 178, 655, 682 |
| BpmI | CTGGAG | 2 cut(s) | 560, 629 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 192 |
| BpuMI | CCSGG | 3 cut(s) | 198, 720, 813 |
| BsaHI | GRCGYC | 1 cut(s) | 733 |
| BsaJI | CCNNGG | 2 cut(s) | 147, 527 |
| BsaWI | WCCGGW | 2 cut(s) | 214, 659 |
| Bse118I | RCCGGY | 2 cut(s) | 214, 659 |
| Bse1I | ACTGG | 2 cut(s) | 255, 436 |
| BseBI | CCWGG | 5 cut(s) | 149, 529, 647, 770, 791 |
| BseDI | CCNNGG | 2 cut(s) | 147, 527 |
| BseGI | GGATG | 5 cut(s) | 58, 193, 504, 697, 805 |
| BseMII | CTCAG | 3 cut(s) | 18, 206, 588 |
| BseNI | ACTGG | 2 cut(s) | 255, 436 |
| BseRI | GAGGAG | 2 cut(s) | 44, 738 |
| BseSI | GKGCMC | 1 cut(s) | 506 |
| BseXI | GCAGC | 2 cut(s) | 213, 216 |
| BseYI | CCCAGC | 2 cut(s) | 134, 737 |
| BsgI | GTGCAG | 1 cut(s) | 239 |
| Bsh1236I | CGCG | 2 cut(s) | 282, 403 |
| BshFI | GGCC | 6 cut(s) | 311, 326, 504, 632, 655, 773 |
| BshNI | GGYRCC | 3 cut(s) | 442, 469, 732 |
| BshTI | ACCGGT | 2 cut(s) | 214, 659 |
| BsiHKAI | GWGCWC | 1 cut(s) | 410 |
| BsiSI | CCGG | 5 cut(s) | 197, 215, 660, 719, 813 |
| BsnI | GGCC | 6 cut(s) | 311, 326, 504, 632, 655, 773 |
| Bsp120I | GGGCCC | 1 cut(s) | 502 |
| Bsp1286I | GDGCHC | 2 cut(s) | 410, 506 |
| Bsp143I | GATC | 4 cut(s) | 40, 126, 162, 793 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 192 |
| BspANI | GGCC | 6 cut(s) | 311, 326, 504, 632, 655, 773 |
| BspCNI | CTCAG | 3 cut(s) | 19, 205, 589 |
| BspFNI | CGCG | 2 cut(s) | 282, 403 |
| BspLI | GGNNCC | 7 cut(s) | 144, 444, 471, 504, 654, 734, 795 |
| BspMI | ACCTGC | 5 cut(s) | 310, 457, 517, 526, 619 |
| BspPI | GGATC | 4 cut(s) | 48, 157, 788, 801 |
| BspT107I | GGYRCC | 3 cut(s) | 442, 469, 732 |
| BsrBI | CCGCTC | 1 cut(s) | 576 |
| BsrFI | RCCGGY | 2 cut(s) | 214, 659 |
| BsrI | ACTGG | 2 cut(s) | 255, 436 |
| BssAI | RCCGGY | 2 cut(s) | 214, 659 |
| BssECI | CCNNGG | 2 cut(s) | 147, 527 |
| BssMI | GATC | 4 cut(s) | 40, 126, 162, 793 |
| BssNI | GRCGYC | 1 cut(s) | 733 |
| Bst2UI | CCWGG | 5 cut(s) | 149, 529, 647, 770, 791 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 107 |
| Bst6I | CTCTTC | 1 cut(s) | 765 |
| BstACI | GRCGYC | 1 cut(s) | 733 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 246, 395, 630 |
| BstDEI | CTNAG | 3 cut(s) | 27, 192, 597 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 5 cut(s) | 58, 193, 504, 697, 805 |
| BstFNI | CGCG | 2 cut(s) | 282, 403 |
| BstH2I | RGCGCY | 2 cut(s) | 365, 736 |
| BstHHI | GCGC | 4 cut(s) | 282, 294, 364, 735 |
| BstKTI | GATC | 4 cut(s) | 43, 129, 165, 796 |
| BstMBI | GATC | 4 cut(s) | 40, 126, 162, 793 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 207, 368, 394, 414, 472, 518, 587 |
| BstNI | CCWGG | 5 cut(s) | 149, 529, 647, 770, 791 |
| BstNSI | RCATGY | 2 cut(s) | 466, 804 |
| BstSCI | CCNGG | 8 cut(s) | 147, 196, 527, 645, 718, 768, 789, 811 |
| BstSLI | GKGCMC | 1 cut(s) | 506 |
| BstUI | CGCG | 2 cut(s) | 282, 403 |
| BstV1I | GCAGC | 2 cut(s) | 213, 216 |
| BstX2I | RGATCY | 2 cut(s) | 162, 793 |
| BstYI | RGATCY | 2 cut(s) | 162, 793 |
| BsuRI | GGCC | 6 cut(s) | 311, 326, 504, 632, 655, 773 |
| BtrI | CACGTC | 1 cut(s) | 766 |
| BtsCI | GGATG | 5 cut(s) | 58, 193, 504, 697, 805 |
| BtsIMutI | CAGTG | 1 cut(s) | 248 |
| BveI | ACCTGC | 5 cut(s) | 310, 457, 517, 526, 619 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 246, 395, 630 |
| CfoI | GCGC | 4 cut(s) | 282, 294, 364, 735 |
| Cfr10I | RCCGGY | 2 cut(s) | 214, 659 |
| Cfr13I | GGNCC | 6 cut(s) | 256, 310, 502, 503, 631, 653 |
| CseI | GACGC | 1 cut(s) | 423 |
| Csp6I | GTAC | 4 cut(s) | 443, 458, 470, 818 |
| CspAI | ACCGGT | 2 cut(s) | 214, 659 |
| CviAII | CATG | 4 cut(s) | 287, 463, 801, 854 |
| CviQI | GTAC | 4 cut(s) | 443, 458, 470, 818 |
| DdeI | CTNAG | 3 cut(s) | 27, 192, 597 |
| DinI | GGCGCC | 1 cut(s) | 734 |
| DpnI | GATC | 4 cut(s) | 42, 128, 164, 795 |
| DpnII | GATC | 4 cut(s) | 40, 126, 162, 793 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| Eam1104I | CTCTTC | 1 cut(s) | 765 |
| EarI | CTCTTC | 1 cut(s) | 765 |
| EciI | GGCGGA | 4 cut(s) | 37, 320, 450, 798 |
| Eco24I | GRGCYC | 1 cut(s) | 506 |
| Eco47I | GGWCC | 1 cut(s) | 256 |
| Eco57I | CTGAAG | 2 cut(s) | 374, 656 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 653 |
| EcoRII | CCWGG | 5 cut(s) | 147, 527, 645, 768, 789 |
| EcoT38I | GRGCYC | 1 cut(s) | 506 |
| EgeI | GGCGCC | 1 cut(s) | 734 |
| EheI | GGCGCC | 1 cut(s) | 734 |
| FaeI | CATG | 4 cut(s) | 290, 466, 804, 857 |
| FaiI | YATR | 6 cut(s) | 236, 240, 288, 464, 802, 855 |
| FatI | CATG | 4 cut(s) | 286, 462, 800, 853 |
| FauI | CCCGC | 4 cut(s) | 8, 174, 615, 643 |
| Fnu4HI | GCNGC | 4 cut(s) | 202, 205, 208, 851 |
| FokI | GGATG | 5 cut(s) | 45, 200, 511, 704, 792 |
| FriOI | GRGCYC | 1 cut(s) | 506 |
| Fsp4HI | GCNGC | 4 cut(s) | 202, 205, 208, 851 |
| FspBI | CTAG | 2 cut(s) | 591, 702 |
| GlaI | GCGC | 4 cut(s) | 281, 293, 363, 734 |
| GluI | GCNGC | 4 cut(s) | 202, 205, 208, 851 |
| GsaI | CCCAGC | 2 cut(s) | 138, 741 |
| GsuI | CTGGAG | 2 cut(s) | 560, 629 |
| HaeII | RGCGCY | 2 cut(s) | 365, 736 |
| HaeIII | GGCC | 6 cut(s) | 311, 326, 504, 632, 655, 773 |
| HapII | CCGG | 5 cut(s) | 197, 215, 660, 719, 813 |
| HgaI | GACGC | 1 cut(s) | 423 |
| HhaI | GCGC | 4 cut(s) | 282, 294, 364, 735 |
| Hin1I | GRCGYC | 1 cut(s) | 733 |
| Hin1II | CATG | 4 cut(s) | 290, 466, 804, 857 |
| Hin6I | GCGC | 4 cut(s) | 280, 292, 362, 733 |
| HinP1I | GCGC | 4 cut(s) | 280, 292, 362, 733 |
| HincII | GTYRAC | 2 cut(s) | 103, 422 |
| HindII | GTYRAC | 2 cut(s) | 103, 422 |
| HinfI | GANTC | 1 cut(s) | 554 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 5 cut(s) | 197, 215, 660, 719, 813 |
| HphI | GGTGA | 2 cut(s) | 674, 776 |
| Hpy166II | GTNNAC | 5 cut(s) | 103, 256, 422, 460, 820 |
| Hpy8I | GTNNAC | 5 cut(s) | 103, 256, 422, 460, 820 |
| HpyAV | CCTTC | 3 cut(s) | 269, 352, 541 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 107 |
| HpyCH4IV | ACGT | 3 cut(s) | 81, 329, 765 |
| HpyCH4V | TGCA | 5 cut(s) | 110, 220, 466, 668, 695 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 207, 368, 394, 414, 472, 518, 587 |
| HpyF3I | CTNAG | 3 cut(s) | 27, 192, 597 |
| HpySE526I | ACGT | 3 cut(s) | 81, 329, 765 |
| Hsp92I | GRCGYC | 1 cut(s) | 733 |
| Hsp92II | CATG | 4 cut(s) | 290, 466, 804, 857 |
| HspAI | GCGC | 4 cut(s) | 280, 292, 362, 733 |
| KasI | GGCGCC | 1 cut(s) | 732 |
| KpnI | GGTACC | 2 cut(s) | 446, 473 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 4 cut(s) | 40, 126, 162, 793 |
| LmnI | GCTCC | 5 cut(s) | 31, 405, 541, 625, 746 |
| Lsp1109I | GCAGC | 2 cut(s) | 213, 216 |
| LweI | GCATC | 3 cut(s) | 178, 655, 682 |
| MaeI | CTAG | 2 cut(s) | 591, 702 |
| MaeII | ACGT | 3 cut(s) | 81, 329, 765 |
| MaeIII | GTNAC | 3 cut(s) | 151, 555, 838 |
| MalI | GATC | 4 cut(s) | 42, 128, 164, 795 |
| MbiI | CCGCTC | 1 cut(s) | 576 |
| MboI | GATC | 4 cut(s) | 40, 126, 162, 793 |
| MboII | GAAGA | 3 cut(s) | 367, 649, 752 |
| MflI | RGATCY | 2 cut(s) | 162, 793 |
| MhlI | GDGCHC | 2 cut(s) | 410, 506 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 1 cut(s) | 341 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| Mly113I | GGCGCC | 1 cut(s) | 733 |
| MlyI | GAGTC | 1 cut(s) | 563 |
| MmeI | TCCRAC | 1 cut(s) | 407 |
| MnlI | CCTC | 9 cut(s) | 22, 133, 241, 301, 592, 625, 653, 716, 719 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 677 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 3 cut(s) | 185, 475, 850 |
| MspI | CCGG | 5 cut(s) | 197, 215, 660, 719, 813 |
| MspR9I | CCNGG | 8 cut(s) | 149, 198, 529, 647, 720, 770, 791, 813 |
| MvaI | CCWGG | 5 cut(s) | 149, 529, 647, 770, 791 |
| MvnI | CGCG | 2 cut(s) | 282, 403 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 207, 368, 394, 414, 472, 518, 587 |
| NarI | GGCGCC | 1 cut(s) | 733 |
| NciI | CCSGG | 3 cut(s) | 198, 720, 813 |
| NdeII | GATC | 4 cut(s) | 40, 126, 162, 793 |
| NlaIII | CATG | 4 cut(s) | 290, 466, 804, 857 |
| NlaIV | GGNNCC | 7 cut(s) | 144, 444, 471, 504, 654, 734, 795 |
| NmeAIII | GCCGAG | 1 cut(s) | 557 |
| NmuCI | GTSAC | 2 cut(s) | 555, 838 |
| NspI | RCATGY | 2 cut(s) | 466, 804 |
| PaqCI | CACCTGC | 4 cut(s) | 310, 517, 526, 619 |
| PflFI | GACNNNGTC | 1 cut(s) | 417 |
| PfoI | TCCNGGA | 1 cut(s) | 718 |
| PinAI | ACCGGT | 2 cut(s) | 214, 659 |
| PkrI | GCNGC | 4 cut(s) | 203, 206, 209, 852 |
| PleI | GAGTC | 1 cut(s) | 562 |
| PluTI | GGCGCC | 1 cut(s) | 736 |
| PpsI | GAGTC | 1 cut(s) | 562 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp6I | CCWGG | 5 cut(s) | 147, 527, 645, 768, 789 |
| PspFI | CCCAGC | 2 cut(s) | 134, 737 |
| PspGI | CCWGG | 5 cut(s) | 147, 527, 645, 768, 789 |
| PspN4I | GGNNCC | 7 cut(s) | 144, 444, 471, 504, 654, 734, 795 |
| PspOMI | GGGCCC | 1 cut(s) | 502 |
| PspPI | GGNCC | 6 cut(s) | 256, 310, 502, 503, 631, 653 |
| PsuI | RGATCY | 2 cut(s) | 162, 793 |
| PsyI | GACNNNGTC | 1 cut(s) | 417 |
| RsaI | GTAC | 4 cut(s) | 444, 459, 471, 819 |
| RsaNI | GTAC | 4 cut(s) | 443, 458, 470, 818 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 677 |
| SatI | GCNGC | 4 cut(s) | 202, 205, 208, 851 |
| Sau3AI | GATC | 4 cut(s) | 40, 126, 162, 793 |
| Sau96I | GGNCC | 6 cut(s) | 256, 310, 502, 503, 631, 653 |
| SchI | GAGTC | 1 cut(s) | 563 |
| ScrFI | CCNGG | 8 cut(s) | 149, 198, 529, 647, 720, 770, 791, 813 |
| SduI | GDGCHC | 2 cut(s) | 410, 506 |
| SfaNI | GCATC | 3 cut(s) | 178, 655, 682 |
| SfoI | GGCGCC | 1 cut(s) | 734 |
| SgrAI | CRCCGGYG | 1 cut(s) | 214 |
| SinI | GGWCC | 1 cut(s) | 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| Sse9I | AATT | 1 cut(s) | 341 |
| SspDI | GGCGCC | 1 cut(s) | 732 |
| SspMI | CTAG | 2 cut(s) | 591, 702 |
| StyD4I | CCNGG | 8 cut(s) | 147, 196, 527, 645, 718, 768, 789, 811 |
| TaaI | ACNGT | 2 cut(s) | 11, 107 |
| TaiI | ACGT | 3 cut(s) | 84, 332, 768 |
| TasI | AATT | 1 cut(s) | 341 |
| TatI | WGTACW | 1 cut(s) | 457 |
| TauI | GCSGC | 2 cut(s) | 210, 853 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 677 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 677 |
| TscAI | CASTG | 1 cut(s) | 255 |
| TseFI | GTSAC | 2 cut(s) | 555, 838 |
| TseI | GCWGC | 2 cut(s) | 201, 204 |
| Tsp45I | GTSAC | 2 cut(s) | 555, 838 |
| TspRI | CASTG | 1 cut(s) | 255 |
| Tth111I | GACNNNGTC | 1 cut(s) | 417 |
| VpaK11BI | GGWCC | 1 cut(s) | 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 1 cut(s) | 341 |
| XceI | RCATGY | 2 cut(s) | 466, 804 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 535 |
| XspI | CTAG | 2 cut(s) | 591, 702 |