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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 74 |
| AciI | CCGC | 9 cut(s) | 15, 38, 72, 74, 146, 167, 185, 231, 254 |
| AclWI | GGATC | 2 cut(s) | 48, 264 |
| AgsI | TTSAA | 2 cut(s) | 118, 347 |
| AjnI | CCWGG | 1 cut(s) | 196 |
| AlwI | GGATC | 2 cut(s) | 48, 264 |
| AoxI | GGCC | 2 cut(s) | 309, 325 |
| ApeKI | GCWGC | 1 cut(s) | 201 |
| AspS9I | GGNCC | 3 cut(s) | 223, 310, 325 |
| AsuC2I | CCSGG | 2 cut(s) | 135, 216 |
| AsuNHI | GCTAGC | 1 cut(s) | 77 |
| AvaII | GGWCC | 1 cut(s) | 223 |
| BbvI | GCAGC | 1 cut(s) | 213 |
| BccI | CCATC | 2 cut(s) | 66, 336 |
| BciT130I | CCWGG | 1 cut(s) | 198 |
| BcnI | CCSGG | 2 cut(s) | 135, 216 |
| BfaI | CTAG | 2 cut(s) | 78, 251 |
| BfmI | CTRYAG | 1 cut(s) | 10 |
| BisI | GCNGC | 4 cut(s) | 75, 146, 202, 231 |
| BlsI | GCNGC | 4 cut(s) | 76, 147, 203, 232 |
| Bme1390I | CCNGG | 3 cut(s) | 135, 198, 216 |
| Bme18I | GGWCC | 1 cut(s) | 223 |
| BmgT120I | GGNCC | 3 cut(s) | 223, 310, 325 |
| BmrFI | CCNGG | 3 cut(s) | 135, 198, 216 |
| BmtI | GCTAGC | 1 cut(s) | 81 |
| BpuMI | CCSGG | 2 cut(s) | 135, 216 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 106, 133 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| Bse1I | ACTGG | 1 cut(s) | 149 |
| BseBI | CCWGG | 1 cut(s) | 198 |
| BseDI | CCNNGG | 3 cut(s) | 72, 106, 133 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 1 cut(s) | 149 |
| BseXI | GCAGC | 1 cut(s) | 213 |
| BseYI | CCCAGC | 1 cut(s) | 88 |
| Bsh1236I | CGCG | 1 cut(s) | 74 |
| BshFI | GGCC | 2 cut(s) | 311, 327 |
| BsiSI | CCGG | 3 cut(s) | 125, 135, 215 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 2 cut(s) | 311, 327 |
| Bsp143I | GATC | 2 cut(s) | 40, 256 |
| BspACI | CCGC | 9 cut(s) | 15, 38, 72, 74, 146, 167, 185, 231, 254 |
| BspANI | GGCC | 2 cut(s) | 311, 327 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 1 cut(s) | 74 |
| BspOI | GCTAGC | 1 cut(s) | 81 |
| BspPI | GGATC | 2 cut(s) | 48, 264 |
| BsrI | ACTGG | 1 cut(s) | 149 |
| BssECI | CCNNGG | 3 cut(s) | 72, 106, 133 |
| BssMI | GATC | 2 cut(s) | 40, 256 |
| Bst2UI | CCWGG | 1 cut(s) | 198 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 107 |
| BstC8I | GCNNGC | 2 cut(s) | 79, 212 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstDSI | CCRYGG | 2 cut(s) | 72, 106 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 1 cut(s) | 74 |
| BstKTI | GATC | 2 cut(s) | 43, 259 |
| BstMBI | GATC | 2 cut(s) | 40, 256 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 207 |
| BstNI | CCWGG | 1 cut(s) | 198 |
| BstSCI | CCNGG | 3 cut(s) | 133, 196, 214 |
| BstSFI | CTRYAG | 1 cut(s) | 10 |
| BstUI | CGCG | 1 cut(s) | 74 |
| BstV1I | GCAGC | 1 cut(s) | 213 |
| BstX2I | RGATCY | 2 cut(s) | 40, 256 |
| BstYI | RGATCY | 2 cut(s) | 40, 256 |
| BsuRI | GGCC | 2 cut(s) | 311, 327 |
| BtgI | CCRYGG | 2 cut(s) | 72, 106 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 1 cut(s) | 24 |
| Cac8I | GCNNGC | 2 cut(s) | 79, 212 |
| Cfr13I | GGNCC | 3 cut(s) | 223, 310, 325 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| CviJI | RGCY | 5 cut(s) | 77, 145, 214, 311, 327 |
| CviKI_1 | RGCY | 5 cut(s) | 77, 145, 214, 311, 327 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 258 |
| DpnII | GATC | 2 cut(s) | 40, 256 |
| Eco47I | GGWCC | 1 cut(s) | 223 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| EcoRII | CCWGG | 1 cut(s) | 196 |
| FaiI | YATR | 4 cut(s) | 177, 179, 236, 240 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| FauI | CCCGC | 3 cut(s) | 8, 79, 174 |
| Fnu4HI | GCNGC | 4 cut(s) | 75, 146, 202, 231 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 4 cut(s) | 75, 146, 202, 231 |
| FspBI | CTAG | 2 cut(s) | 78, 251 |
| GluI | GCNGC | 4 cut(s) | 75, 146, 202, 231 |
| GsaI | CCCAGC | 1 cut(s) | 92 |
| HaeIII | GGCC | 2 cut(s) | 311, 327 |
| HapII | CCGG | 3 cut(s) | 125, 135, 215 |
| HincII | GTYRAC | 1 cut(s) | 130 |
| HindII | GTYRAC | 1 cut(s) | 130 |
| HpaI | GTTAAC | 1 cut(s) | 130 |
| HpaII | CCGG | 3 cut(s) | 125, 135, 215 |
| Hpy166II | GTNNAC | 2 cut(s) | 110, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 221 |
| Hpy8I | GTNNAC | 2 cut(s) | 110, 130 |
| HpyAV | CCTTC | 1 cut(s) | 295 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 107 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 207 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| KspAI | GTTAAC | 1 cut(s) | 130 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 40, 256 |
| Lsp1109I | GCAGC | 1 cut(s) | 213 |
| MaeI | CTAG | 2 cut(s) | 78, 251 |
| MaeIII | GTNAC | 1 cut(s) | 151 |
| MalI | GATC | 2 cut(s) | 42, 258 |
| MboI | GATC | 2 cut(s) | 40, 256 |
| MflI | RGATCY | 2 cut(s) | 40, 256 |
| MluCI | AATT | 1 cut(s) | 99 |
| MnlI | CCTC | 3 cut(s) | 22, 301, 326 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 129 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 185 |
| MspI | CCGG | 3 cut(s) | 125, 135, 215 |
| MspR9I | CCNGG | 3 cut(s) | 135, 198, 216 |
| MvaI | CCWGG | 1 cut(s) | 198 |
| MvnI | CGCG | 1 cut(s) | 74 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 207 |
| NciI | CCSGG | 2 cut(s) | 135, 216 |
| NdeII | GATC | 2 cut(s) | 40, 256 |
| NheI | GCTAGC | 1 cut(s) | 77 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 337 |
| PkrI | GCNGC | 4 cut(s) | 76, 147, 203, 232 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| Psp6I | CCWGG | 1 cut(s) | 196 |
| PspFI | CCCAGC | 1 cut(s) | 88 |
| PspGI | CCWGG | 1 cut(s) | 196 |
| PspPI | GGNCC | 3 cut(s) | 223, 310, 325 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| PsuI | RGATCY | 2 cut(s) | 40, 256 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 129 |
| SatI | GCNGC | 4 cut(s) | 75, 146, 202, 231 |
| Sau3AI | GATC | 2 cut(s) | 40, 256 |
| Sau96I | GGNCC | 3 cut(s) | 223, 310, 325 |
| ScrFI | CCNGG | 3 cut(s) | 135, 198, 216 |
| SetI | ASST | 3 cut(s) | 199, 228, 337 |
| SfcI | CTRYAG | 1 cut(s) | 10 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 1 cut(s) | 223 |
| Sse9I | AATT | 1 cut(s) | 99 |
| SsiI | CCGC | 9 cut(s) | 15, 38, 72, 74, 146, 167, 185, 231, 254 |
| SspMI | CTAG | 2 cut(s) | 78, 251 |
| StyD4I | CCNGG | 3 cut(s) | 133, 196, 214 |
| TaaI | ACNGT | 2 cut(s) | 11, 107 |
| TaqI | TCGA | 1 cut(s) | 331 |
| TasI | AATT | 1 cut(s) | 99 |
| TauI | GCSGC | 3 cut(s) | 77, 148, 233 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 129 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 129 |
| TscAI | CASTG | 1 cut(s) | 31 |
| TseI | GCWGC | 1 cut(s) | 201 |
| TspRI | CASTG | 1 cut(s) | 31 |
| VpaK11BI | GGWCC | 1 cut(s) | 223 |
| XspI | CTAG | 2 cut(s) | 78, 251 |