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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 184 |
| Acc36I | ACCTGC | 1 cut(s) | 184 |
| AccBSI | CCGCTC | 1 cut(s) | 302 |
| AccII | CGCG | 1 cut(s) | 64 |
| AciI | CCGC | 7 cut(s) | 77, 82, 105, 277, 289, 300, 363 |
| AcoI | YGGCCR | 1 cut(s) | 198 |
| AcsI | RAATTY | 1 cut(s) | 215 |
| AcuI | CTGAAG | 1 cut(s) | 248 |
| AdeI | CACNNNGTG | 1 cut(s) | 266 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 388 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 66 |
| AhlI | ACTAGT | 1 cut(s) | 124 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 221, 253 |
| AluBI | AGCT | 3 cut(s) | 118, 226, 354 |
| AluI | AGCT | 3 cut(s) | 118, 226, 354 |
| AoxI | GGCC | 1 cut(s) | 198 |
| ApoI | RAATTY | 1 cut(s) | 215 |
| AspA2I | CCTAGG | 1 cut(s) | 343 |
| AspLEI | GCGC | 1 cut(s) | 66 |
| AspS9I | GGNCC | 3 cut(s) | 97, 130, 379 |
| AvaII | GGWCC | 3 cut(s) | 97, 130, 379 |
| AvrII | CCTAGG | 1 cut(s) | 343 |
| BalI | TGGCCA | 1 cut(s) | 200 |
| BceAI | ACGGC | 1 cut(s) | 51 |
| BcuI | ACTAGT | 1 cut(s) | 124 |
| BfaI | CTAG | 3 cut(s) | 125, 344, 372 |
| BfuAI | ACCTGC | 1 cut(s) | 184 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 19 |
| BisI | GCNGC | 4 cut(s) | 77, 105, 289, 300 |
| BlnI | CCTAGG | 1 cut(s) | 343 |
| BlsI | GCNGC | 4 cut(s) | 78, 106, 290, 301 |
| Bme18I | GGWCC | 3 cut(s) | 97, 130, 379 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 66 |
| BmgT120I | GGNCC | 3 cut(s) | 97, 130, 379 |
| BmiI | GGNNCC | 2 cut(s) | 380, 381 |
| BmsI | GCATC | 1 cut(s) | 377 |
| BpmI | CTGGAG | 1 cut(s) | 72 |
| Bpu10I | CCTNAGC | 1 cut(s) | 30 |
| BsaJI | CCNNGG | 2 cut(s) | 35, 343 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 388 |
| Bse1I | ACTGG | 1 cut(s) | 89 |
| BseDI | CCNNGG | 2 cut(s) | 35, 343 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 388 |
| BseMII | CTCAG | 2 cut(s) | 21, 314 |
| BseNI | ACTGG | 1 cut(s) | 89 |
| BseRI | GAGGAG | 1 cut(s) | 284 |
| BseYI | CCCAGC | 2 cut(s) | 256, 295 |
| BsgI | GTGCAG | 2 cut(s) | 113, 187 |
| Bsh1236I | CGCG | 1 cut(s) | 64 |
| BshFI | GGCC | 1 cut(s) | 200 |
| BslFI | GGGAC | 2 cut(s) | 325, 392 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 388 |
| BsmFI | GGGAC | 2 cut(s) | 325, 392 |
| BsnI | GGCC | 1 cut(s) | 200 |
| Bsp143I | GATC | 1 cut(s) | 50 |
| BspACI | CCGC | 7 cut(s) | 77, 82, 105, 277, 289, 300, 363 |
| BspANI | GGCC | 1 cut(s) | 200 |
| BspCNI | CTCAG | 2 cut(s) | 22, 315 |
| BspFNI | CGCG | 1 cut(s) | 64 |
| BspLI | GGNNCC | 2 cut(s) | 380, 381 |
| BspMI | ACCTGC | 1 cut(s) | 184 |
| BsrBI | CCGCTC | 1 cut(s) | 302 |
| BsrI | ACTGG | 1 cut(s) | 89 |
| BssECI | CCNNGG | 2 cut(s) | 35, 343 |
| BssMI | GATC | 1 cut(s) | 50 |
| BssT1I | CCWWGG | 2 cut(s) | 35, 343 |
| Bst4CI | ACNGT | 1 cut(s) | 309 |
| Bst6I | CTCTTC | 1 cut(s) | 224 |
| BstC8I | GCNNGC | 4 cut(s) | 20, 42, 120, 158 |
| BstDEI | CTNAG | 2 cut(s) | 30, 323 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 137 |
| BstFNI | CGCG | 1 cut(s) | 64 |
| BstHHI | GCGC | 1 cut(s) | 66 |
| BstKTI | GATC | 1 cut(s) | 53 |
| BstMBI | GATC | 1 cut(s) | 50 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 19, 242, 257, 360 |
| BstUI | CGCG | 1 cut(s) | 64 |
| BsuRI | GGCC | 1 cut(s) | 200 |
| BtsI | GCAGTG | 1 cut(s) | 53 |
| BtsIMutI | CAGTG | 2 cut(s) | 53, 96 |
| BveI | ACCTGC | 1 cut(s) | 184 |
| Cac8I | GCNNGC | 4 cut(s) | 20, 42, 120, 158 |
| CfoI | GCGC | 1 cut(s) | 66 |
| Cfr13I | GGNCC | 3 cut(s) | 97, 130, 379 |
| CseI | GACGC | 1 cut(s) | 70 |
| CviAII | CATG | 1 cut(s) | 202 |
| DdeI | CTNAG | 2 cut(s) | 30, 323 |
| DpnI | GATC | 1 cut(s) | 52 |
| DpnII | GATC | 1 cut(s) | 50 |
| DraIII | CACNNNGTG | 1 cut(s) | 266 |
| DriI | GACNNNNNGTC | 1 cut(s) | 66 |
| EaeI | YGGCCR | 1 cut(s) | 198 |
| Eam1104I | CTCTTC | 1 cut(s) | 224 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 66 |
| EarI | CTCTTC | 1 cut(s) | 224 |
| Eco130I | CCWWGG | 2 cut(s) | 35, 343 |
| Eco47I | GGWCC | 3 cut(s) | 97, 130, 379 |
| Eco57I | CTGAAG | 1 cut(s) | 248 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 137 |
| EcoO109I | RGGNCCY | 2 cut(s) | 97, 379 |
| EcoT14I | CCWWGG | 2 cut(s) | 35, 343 |
| ErhI | CCWWGG | 2 cut(s) | 35, 343 |
| FaeI | CATG | 1 cut(s) | 205 |
| FaiI | YATR | 6 cut(s) | 110, 114, 203, 334, 386, 394 |
| FaqI | GGGAC | 2 cut(s) | 325, 392 |
| FatI | CATG | 1 cut(s) | 201 |
| Fnu4HI | GCNGC | 4 cut(s) | 77, 105, 289, 300 |
| Fsp4HI | GCNGC | 4 cut(s) | 77, 105, 289, 300 |
| FspBI | CTAG | 3 cut(s) | 125, 344, 372 |
| GlaI | GCGC | 1 cut(s) | 65 |
| GluI | GCNGC | 4 cut(s) | 77, 105, 289, 300 |
| GsaI | CCCAGC | 2 cut(s) | 260, 299 |
| GsuI | CTGGAG | 1 cut(s) | 72 |
| HaeIII | GGCC | 1 cut(s) | 200 |
| HgaI | GACGC | 1 cut(s) | 70 |
| HhaI | GCGC | 1 cut(s) | 66 |
| Hin1II | CATG | 1 cut(s) | 205 |
| Hin6I | GCGC | 1 cut(s) | 64 |
| HinP1I | GCGC | 1 cut(s) | 64 |
| HinfI | GANTC | 1 cut(s) | 280 |
| Hpy166II | GTNNAC | 1 cut(s) | 130 |
| Hpy188III | TCNNGA | 2 cut(s) | 71, 149 |
| Hpy8I | GTNNAC | 1 cut(s) | 130 |
| HpyAV | CCTTC | 1 cut(s) | 143 |
| HpyCH4III | ACNGT | 1 cut(s) | 309 |
| HpyCH4V | TGCA | 2 cut(s) | 94, 168 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 19, 242, 257, 360 |
| HpyF3I | CTNAG | 2 cut(s) | 30, 323 |
| Hsp92II | CATG | 1 cut(s) | 205 |
| HspAI | GCGC | 1 cut(s) | 64 |
| KflI | GGGWCCC | 1 cut(s) | 379 |
| Kzo9I | GATC | 1 cut(s) | 50 |
| LmnI | GCTCC | 1 cut(s) | 365 |
| LweI | GCATC | 1 cut(s) | 377 |
| MaeI | CTAG | 3 cut(s) | 125, 344, 372 |
| MaeIII | GTNAC | 1 cut(s) | 281 |
| MalI | GATC | 1 cut(s) | 52 |
| MbiI | CCGCTC | 1 cut(s) | 302 |
| MboI | GATC | 1 cut(s) | 50 |
| MboII | GAAGA | 1 cut(s) | 241 |
| MlsI | TGGCCA | 1 cut(s) | 200 |
| MluCI | AATT | 1 cut(s) | 215 |
| MluNI | TGGCCA | 1 cut(s) | 200 |
| MlyI | GAGTC | 1 cut(s) | 289 |
| MmeI | TCCRAC | 1 cut(s) | 219 |
| MnlI | CCTC | 8 cut(s) | 96, 110, 175, 225, 262, 318, 369, 382 |
| Mox20I | TGGCCA | 1 cut(s) | 200 |
| MscI | TGGCCA | 1 cut(s) | 200 |
| MslI | CAYNNNNRTG | 1 cut(s) | 206 |
| Msp20I | TGGCCA | 1 cut(s) | 200 |
| MspA1I | CMGCKG | 1 cut(s) | 363 |
| MvnI | CGCG | 1 cut(s) | 64 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 19, 242, 257, 360 |
| NdeII | GATC | 1 cut(s) | 50 |
| NlaIII | CATG | 1 cut(s) | 205 |
| NlaIV | GGNNCC | 2 cut(s) | 380, 381 |
| NmuCI | GTSAC | 1 cut(s) | 281 |
| PaqCI | CACCTGC | 1 cut(s) | 184 |
| PkrI | GCNGC | 4 cut(s) | 78, 106, 290, 301 |
| PleI | GAGTC | 1 cut(s) | 288 |
| PpsI | GAGTC | 1 cut(s) | 288 |
| PpuMI | RGGWCCY | 2 cut(s) | 97, 379 |
| Psp5II | RGGWCCY | 2 cut(s) | 97, 379 |
| PspFI | CCCAGC | 2 cut(s) | 256, 295 |
| PspN4I | GGNNCC | 2 cut(s) | 380, 381 |
| PspPI | GGNCC | 3 cut(s) | 97, 130, 379 |
| PspPPI | RGGWCCY | 2 cut(s) | 97, 379 |
| RseI | CAYNNNNRTG | 1 cut(s) | 206 |
| SatI | GCNGC | 4 cut(s) | 77, 105, 289, 300 |
| Sau3AI | GATC | 1 cut(s) | 50 |
| Sau96I | GGNCC | 3 cut(s) | 97, 130, 379 |
| SchI | GAGTC | 1 cut(s) | 289 |
| SetI | ASST | 8 cut(s) | 31, 102, 120, 135, 198, 228, 339, 356 |
| SfaNI | GCATC | 1 cut(s) | 377 |
| SinI | GGWCC | 3 cut(s) | 97, 130, 379 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 206 |
| SpeI | ACTAGT | 1 cut(s) | 124 |
| Sse9I | AATT | 1 cut(s) | 215 |
| SsiI | CCGC | 7 cut(s) | 77, 82, 105, 277, 289, 300, 363 |
| SspMI | CTAG | 3 cut(s) | 125, 344, 372 |
| StyI | CCWWGG | 2 cut(s) | 35, 343 |
| TaaI | ACNGT | 1 cut(s) | 309 |
| TasI | AATT | 1 cut(s) | 215 |
| TauI | GCSGC | 4 cut(s) | 79, 107, 291, 302 |
| TscAI | CASTG | 2 cut(s) | 60, 96 |
| TseFI | GTSAC | 1 cut(s) | 281 |
| Tsp45I | GTSAC | 1 cut(s) | 281 |
| TspRI | CASTG | 2 cut(s) | 60, 96 |
| VpaK11BI | GGWCC | 3 cut(s) | 97, 130, 379 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 137 |
| XapI | RAATTY | 1 cut(s) | 215 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 33 |
| XmaJI | CCTAGG | 1 cut(s) | 343 |
| XspI | CTAG | 3 cut(s) | 125, 344, 372 |