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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 238 |
| Acc36I | ACCTGC | 1 cut(s) | 238 |
| AccII | CGCG | 1 cut(s) | 26 |
| AciI | CCGC | 4 cut(s) | 48, 92, 159, 182 |
| AcoI | YGGCCR | 1 cut(s) | 252 |
| AcuI | CTGAAG | 1 cut(s) | 302 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 139, 229, 247 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AluBI | AGCT | 2 cut(s) | 172, 280 |
| AluI | AGCT | 2 cut(s) | 172, 280 |
| AoxI | GGCC | 2 cut(s) | 237, 252 |
| AspLEI | GCGC | 2 cut(s) | 222, 316 |
| AspS9I | GGNCC | 2 cut(s) | 184, 238 |
| AvaII | GGWCC | 1 cut(s) | 184 |
| BalI | TGGCCA | 1 cut(s) | 254 |
| BbvCI | CCTCAGC | 1 cut(s) | 234 |
| BceAI | ACGGC | 1 cut(s) | 122 |
| BfoI | RGCGCY | 1 cut(s) | 223 |
| BfuAI | ACCTGC | 1 cut(s) | 238 |
| BisI | GCNGC | 1 cut(s) | 159 |
| BlsI | GCNGC | 1 cut(s) | 160 |
| Bme18I | GGWCC | 1 cut(s) | 184 |
| BmgT120I | GGNCC | 2 cut(s) | 184, 238 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BpmI | CTGGAG | 1 cut(s) | 314 |
| Bpu10I | CCTNAGC | 1 cut(s) | 234 |
| BsaJI | CCNNGG | 1 cut(s) | 138 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 139, 229, 247 |
| Bse1I | ACTGG | 2 cut(s) | 129, 183 |
| BseDI | CCNNGG | 1 cut(s) | 138 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 139, 229, 247 |
| BseMII | CTCAG | 1 cut(s) | 225 |
| BseNI | ACTGG | 2 cut(s) | 129, 183 |
| Bsh1236I | CGCG | 1 cut(s) | 26 |
| BshFI | GGCC | 2 cut(s) | 239, 254 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 139, 229, 247 |
| BsnI | GGCC | 2 cut(s) | 239, 254 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 4 cut(s) | 48, 92, 159, 182 |
| BspANI | GGCC | 2 cut(s) | 239, 254 |
| BspCNI | CTCAG | 1 cut(s) | 226 |
| BspFNI | CGCG | 1 cut(s) | 26 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspMI | ACCTGC | 1 cut(s) | 238 |
| BsrI | ACTGG | 2 cut(s) | 129, 183 |
| BssECI | CCNNGG | 1 cut(s) | 138 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 114 |
| Bst6I | CTCTTC | 1 cut(s) | 278 |
| BstC8I | GCNNGC | 1 cut(s) | 36 |
| BstDEI | CTNAG | 1 cut(s) | 234 |
| BstDSI | CCRYGG | 1 cut(s) | 138 |
| BstFNI | CGCG | 1 cut(s) | 26 |
| BstH2I | RGCGCY | 1 cut(s) | 223 |
| BstHHI | GCGC | 2 cut(s) | 222, 316 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 311 |
| BstUI | CGCG | 1 cut(s) | 26 |
| BsuRI | GGCC | 2 cut(s) | 239, 254 |
| BtgI | CCRYGG | 1 cut(s) | 138 |
| BtsIMutI | CAGTG | 3 cut(s) | 110, 122, 176 |
| BveI | ACCTGC | 1 cut(s) | 238 |
| Cac8I | GCNNGC | 1 cut(s) | 36 |
| CfoI | GCGC | 2 cut(s) | 222, 316 |
| Cfr13I | GGNCC | 2 cut(s) | 184, 238 |
| CviAII | CATG | 1 cut(s) | 107 |
| CviJI | RGCY | 5 cut(s) | 172, 233, 239, 254, 280 |
| CviKI_1 | RGCY | 5 cut(s) | 172, 233, 239, 254, 280 |
| DdeI | CTNAG | 1 cut(s) | 234 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 252 |
| Eam1104I | CTCTTC | 1 cut(s) | 278 |
| EarI | CTCTTC | 1 cut(s) | 278 |
| EciI | GGCGGA | 2 cut(s) | 37, 197 |
| Eco47I | GGWCC | 1 cut(s) | 184 |
| Eco57I | CTGAAG | 1 cut(s) | 302 |
| FaeI | CATG | 1 cut(s) | 110 |
| FaiI | YATR | 3 cut(s) | 108, 164, 168 |
| FatI | CATG | 1 cut(s) | 106 |
| Fnu4HI | GCNGC | 1 cut(s) | 159 |
| Fsp4HI | GCNGC | 1 cut(s) | 159 |
| GlaI | GCGC | 2 cut(s) | 221, 315 |
| GluI | GCNGC | 1 cut(s) | 159 |
| GsuI | CTGGAG | 1 cut(s) | 314 |
| HaeII | RGCGCY | 1 cut(s) | 223 |
| HaeIII | GGCC | 2 cut(s) | 239, 254 |
| HhaI | GCGC | 2 cut(s) | 222, 316 |
| Hin1II | CATG | 1 cut(s) | 110 |
| Hin6I | GCGC | 2 cut(s) | 220, 314 |
| HinP1I | GCGC | 2 cut(s) | 220, 314 |
| HincII | GTYRAC | 2 cut(s) | 85, 130 |
| HindII | GTYRAC | 2 cut(s) | 85, 130 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 267 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 130 |
| HpyAV | CCTTC | 2 cut(s) | 22, 197 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 114 |
| HpyCH4V | TGCA | 1 cut(s) | 110 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 311 |
| HpyF3I | CTNAG | 1 cut(s) | 234 |
| Hsp92II | CATG | 1 cut(s) | 110 |
| HspAI | GCGC | 2 cut(s) | 220, 314 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LmnI | GCTCC | 2 cut(s) | 31, 217 |
| LpnPI | CCDG | 6 cut(s) | 110, 161, 164, 200, 233, 278 |
| MalI | GATC | 1 cut(s) | 42 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 1 cut(s) | 295 |
| MlsI | TGGCCA | 1 cut(s) | 254 |
| MluNI | TGGCCA | 1 cut(s) | 254 |
| MnlI | CCTC | 5 cut(s) | 143, 164, 229, 279, 289 |
| Mox20I | TGGCCA | 1 cut(s) | 254 |
| MscI | TGGCCA | 1 cut(s) | 254 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MslI | CAYNNNNRTG | 1 cut(s) | 260 |
| Msp20I | TGGCCA | 1 cut(s) | 254 |
| MvnI | CGCG | 1 cut(s) | 26 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 311 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIII | CATG | 1 cut(s) | 110 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NmeAIII | GCCGAG | 1 cut(s) | 248 |
| PaqCI | CACCTGC | 1 cut(s) | 238 |
| PkrI | GCNGC | 1 cut(s) | 160 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 2 cut(s) | 184, 238 |
| RseI | CAYNNNNRTG | 1 cut(s) | 260 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 1 cut(s) | 159 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 2 cut(s) | 184, 238 |
| SetI | ASST | 6 cut(s) | 135, 156, 174, 189, 252, 282 |
| SinI | GGWCC | 1 cut(s) | 184 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 260 |
| SsiI | CCGC | 4 cut(s) | 48, 92, 159, 182 |
| TaaI | ACNGT | 2 cut(s) | 11, 114 |
| TaqI | TCGA | 1 cut(s) | 70 |
| TauI | GCSGC | 1 cut(s) | 161 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TscAI | CASTG | 3 cut(s) | 117, 129, 183 |
| TspRI | CASTG | 3 cut(s) | 117, 129, 183 |
| VpaK11BI | GGWCC | 1 cut(s) | 184 |