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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 445, 453 |
| AccBSI | CCGCTC | 1 cut(s) | 388 |
| AccII | CGCG | 2 cut(s) | 79, 137 |
| AclWI | GGATC | 2 cut(s) | 61, 362 |
| AcoI | YGGCCR | 1 cut(s) | 121 |
| AcyI | GRCGYC | 1 cut(s) | 454 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 346, 349, 350, 351, 488 |
| AgsI | TTSAA | 1 cut(s) | 464 |
| AjnI | CCWGG | 4 cut(s) | 11, 345, 481, 486 |
| AluBI | AGCT | 4 cut(s) | 41, 149, 225, 517 |
| AluI | AGCT | 4 cut(s) | 41, 149, 225, 517 |
| AlwI | GGATC | 2 cut(s) | 61, 362 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 396 |
| Ama87I | CYCGRG | 1 cut(s) | 431 |
| AoxI | GGCC | 7 cut(s) | 14, 100, 106, 121, 309, 484, 490 |
| ApeKI | GCWGC | 2 cut(s) | 210, 408 |
| AspA2I | CCTAGG | 1 cut(s) | 312 |
| AspLEI | GCGC | 4 cut(s) | 79, 139, 215, 456 |
| AspS9I | GGNCC | 3 cut(s) | 107, 238, 490 |
| AsuC2I | CCSGG | 2 cut(s) | 374, 451 |
| AvaI | CYCGRG | 1 cut(s) | 431 |
| AvaII | GGWCC | 1 cut(s) | 238 |
| AvrII | CCTAGG | 1 cut(s) | 312 |
| BaeGI | GKGCMC | 1 cut(s) | 254 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 115, 148 |
| BalI | TGGCCA | 1 cut(s) | 123 |
| BanI | GGYRCC | 2 cut(s) | 445, 453 |
| BbvI | GCAGC | 2 cut(s) | 197, 420 |
| BciT130I | CCWGG | 4 cut(s) | 13, 347, 483, 488 |
| BciVI | GTATCC | 1 cut(s) | 362 |
| BcnI | CCSGG | 2 cut(s) | 374, 451 |
| BfaI | CTAG | 2 cut(s) | 226, 313 |
| BfoI | RGCGCY | 1 cut(s) | 457 |
| BfuI | GTATCC | 1 cut(s) | 362 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 13 |
| BisI | GCNGC | 3 cut(s) | 5, 211, 409 |
| BlnI | CCTAGG | 1 cut(s) | 312 |
| BlsI | GCNGC | 3 cut(s) | 6, 212, 410 |
| Bme1390I | CCNGG | 6 cut(s) | 13, 347, 374, 451, 483, 488 |
| Bme18I | GGWCC | 1 cut(s) | 238 |
| BmeT110I | CYCGRG | 1 cut(s) | 431 |
| BmgT120I | GGNCC | 3 cut(s) | 107, 238, 490 |
| BmiI | GGNNCC | 2 cut(s) | 447, 455 |
| BmrFI | CCNGG | 6 cut(s) | 13, 347, 374, 451, 483, 488 |
| BmsI | GCATC | 2 cut(s) | 369, 395 |
| BpmI | CTGGAG | 1 cut(s) | 183 |
| BpuEI | CTTGAG | 1 cut(s) | 326 |
| BpuMI | CCSGG | 2 cut(s) | 374, 451 |
| BsaHI | GRCGYC | 1 cut(s) | 454 |
| BsaJI | CCNNGG | 6 cut(s) | 103, 312, 325, 345, 449, 487 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 346, 349, 350, 351, 488 |
| Bse118I | RCCGGY | 1 cut(s) | 525 |
| Bse1I | ACTGG | 1 cut(s) | 401 |
| BseBI | CCWGG | 4 cut(s) | 13, 347, 483, 488 |
| BseDI | CCNNGG | 6 cut(s) | 103, 312, 325, 345, 449, 487 |
| BseGI | GGATG | 1 cut(s) | 410 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 346, 349, 350, 351, 488 |
| BseMII | CTCAG | 1 cut(s) | 403 |
| BseNI | ACTGG | 1 cut(s) | 401 |
| BseSI | GKGCMC | 1 cut(s) | 254 |
| BseXI | GCAGC | 2 cut(s) | 197, 420 |
| BseYI | CCCAGC | 1 cut(s) | 254 |
| BsgI | GTGCAG | 1 cut(s) | 276 |
| Bsh1236I | CGCG | 2 cut(s) | 79, 137 |
| BshFI | GGCC | 7 cut(s) | 16, 102, 108, 123, 311, 486, 492 |
| BshNI | GGYRCC | 2 cut(s) | 445, 453 |
| BsiHKCI | CYCGRG | 1 cut(s) | 431 |
| BsiSI | CCGG | 3 cut(s) | 374, 451, 526 |
| BslFI | GGGAC | 1 cut(s) | 190 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 346, 349, 350, 351, 488 |
| BsmFI | GGGAC | 1 cut(s) | 190 |
| BsnI | GGCC | 7 cut(s) | 16, 102, 108, 123, 311, 486, 492 |
| BsoBI | CYCGRG | 1 cut(s) | 431 |
| Bsp1286I | GDGCHC | 1 cut(s) | 254 |
| Bsp143I | GATC | 2 cut(s) | 53, 354 |
| BspANI | GGCC | 7 cut(s) | 16, 102, 108, 123, 311, 486, 492 |
| BspCNI | CTCAG | 1 cut(s) | 402 |
| BspFNI | CGCG | 2 cut(s) | 79, 137 |
| BspLI | GGNNCC | 2 cut(s) | 447, 455 |
| BspPI | GGATC | 2 cut(s) | 61, 362 |
| BspT107I | GGYRCC | 2 cut(s) | 445, 453 |
| BsrBI | CCGCTC | 1 cut(s) | 388 |
| BsrFI | RCCGGY | 1 cut(s) | 525 |
| BsrI | ACTGG | 1 cut(s) | 401 |
| BssAI | RCCGGY | 1 cut(s) | 525 |
| BssECI | CCNNGG | 6 cut(s) | 103, 312, 325, 345, 449, 487 |
| BssMI | GATC | 2 cut(s) | 53, 354 |
| BssNI | GRCGYC | 1 cut(s) | 454 |
| BssT1I | CCWWGG | 1 cut(s) | 312 |
| Bst2UI | CCWGG | 4 cut(s) | 13, 347, 483, 488 |
| Bst4CI | ACNGT | 1 cut(s) | 196 |
| BstACI | GRCGYC | 1 cut(s) | 454 |
| BstC8I | GCNNGC | 4 cut(s) | 9, 43, 81, 515 |
| BstDEI | CTNAG | 1 cut(s) | 389 |
| BstF5I | GGATG | 1 cut(s) | 410 |
| BstFNI | CGCG | 2 cut(s) | 79, 137 |
| BstH2I | RGCGCY | 1 cut(s) | 457 |
| BstHHI | GCGC | 4 cut(s) | 79, 139, 215, 456 |
| BstKTI | GATC | 2 cut(s) | 56, 357 |
| BstMBI | GATC | 2 cut(s) | 53, 354 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 13, 134, 417 |
| BstNI | CCWGG | 4 cut(s) | 13, 347, 483, 488 |
| BstSCI | CCNGG | 6 cut(s) | 11, 345, 372, 449, 481, 486 |
| BstSLI | GKGCMC | 1 cut(s) | 254 |
| BstUI | CGCG | 2 cut(s) | 79, 137 |
| BstV1I | GCAGC | 2 cut(s) | 197, 420 |
| BstX2I | RGATCY | 1 cut(s) | 53 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 266 |
| BstYI | RGATCY | 1 cut(s) | 53 |
| BsuI | GTATCC | 1 cut(s) | 362 |
| BsuRI | GGCC | 7 cut(s) | 16, 102, 108, 123, 311, 486, 492 |
| BtgZI | GCGATG | 1 cut(s) | 392 |
| BtsCI | GGATG | 1 cut(s) | 410 |
| BtsI | GCAGTG | 2 cut(s) | 288, 497 |
| BtsIMutI | CAGTG | 2 cut(s) | 288, 497 |
| Cac8I | GCNNGC | 4 cut(s) | 9, 43, 81, 515 |
| CaiI | CAGNNNCTG | 1 cut(s) | 396 |
| CfoI | GCGC | 4 cut(s) | 79, 139, 215, 456 |
| Cfr10I | RCCGGY | 1 cut(s) | 525 |
| Cfr13I | GGNCC | 3 cut(s) | 107, 238, 490 |
| CseI | GACGC | 1 cut(s) | 467 |
| DdeI | CTNAG | 1 cut(s) | 389 |
| DinI | GGCGCC | 1 cut(s) | 455 |
| DpnI | GATC | 2 cut(s) | 55, 356 |
| DpnII | GATC | 2 cut(s) | 53, 354 |
| EaeI | YGGCCR | 1 cut(s) | 121 |
| Eco130I | CCWWGG | 1 cut(s) | 312 |
| Eco147I | AGGCCT | 1 cut(s) | 311 |
| Eco47I | GGWCC | 1 cut(s) | 238 |
| Eco88I | CYCGRG | 1 cut(s) | 431 |
| EcoRII | CCWGG | 4 cut(s) | 11, 345, 481, 486 |
| EcoT14I | CCWWGG | 1 cut(s) | 312 |
| EgeI | GGCGCC | 1 cut(s) | 455 |
| EheI | GGCGCC | 1 cut(s) | 455 |
| ErhI | CCWWGG | 1 cut(s) | 312 |
| FaiI | YATR | 4 cut(s) | 35, 37, 264, 531 |
| FaqI | GGGAC | 1 cut(s) | 190 |
| FauI | CCCGC | 5 cut(s) | 16, 215, 343, 347, 358 |
| Fnu4HI | GCNGC | 3 cut(s) | 5, 211, 409 |
| FokI | GGATG | 1 cut(s) | 417 |
| Fsp4HI | GCNGC | 3 cut(s) | 5, 211, 409 |
| FspBI | CTAG | 2 cut(s) | 226, 313 |
| GlaI | GCGC | 4 cut(s) | 78, 138, 214, 455 |
| GluI | GCNGC | 3 cut(s) | 5, 211, 409 |
| GsaI | CCCAGC | 1 cut(s) | 258 |
| GsuI | CTGGAG | 1 cut(s) | 183 |
| HaeII | RGCGCY | 1 cut(s) | 457 |
| HaeIII | GGCC | 7 cut(s) | 16, 102, 108, 123, 311, 486, 492 |
| HapII | CCGG | 3 cut(s) | 374, 451, 526 |
| HgaI | GACGC | 1 cut(s) | 467 |
| HhaI | GCGC | 4 cut(s) | 79, 139, 215, 456 |
| Hin1I | GRCGYC | 1 cut(s) | 454 |
| Hin6I | GCGC | 4 cut(s) | 77, 137, 213, 454 |
| HinP1I | GCGC | 4 cut(s) | 77, 137, 213, 454 |
| HinfI | GANTC | 5 cut(s) | 203, 282, 439, 507, 540 |
| HpaII | CCGG | 3 cut(s) | 374, 451, 526 |
| Hpy188I | TCNGA | 2 cut(s) | 392, 523 |
| HpyCH4III | ACNGT | 1 cut(s) | 196 |
| HpyCH4V | TGCA | 4 cut(s) | 293, 382, 408, 513 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 13, 134, 417 |
| HpyF3I | CTNAG | 1 cut(s) | 389 |
| Hsp92I | GRCGYC | 1 cut(s) | 454 |
| HspAI | GCGC | 4 cut(s) | 77, 137, 213, 454 |
| KasI | GGCGCC | 1 cut(s) | 453 |
| Kzo9I | GATC | 2 cut(s) | 53, 354 |
| LmnI | GCTCC | 1 cut(s) | 417 |
| Lsp1109I | GCAGC | 2 cut(s) | 197, 420 |
| LweI | GCATC | 2 cut(s) | 369, 395 |
| MaeI | CTAG | 2 cut(s) | 226, 313 |
| MaeIII | GTNAC | 1 cut(s) | 196 |
| MalI | GATC | 2 cut(s) | 55, 356 |
| MbiI | CCGCTC | 1 cut(s) | 388 |
| MboI | GATC | 2 cut(s) | 53, 354 |
| MboII | GAAGA | 1 cut(s) | 48 |
| MflI | RGATCY | 1 cut(s) | 53 |
| MhlI | GDGCHC | 1 cut(s) | 254 |
| MlsI | TGGCCA | 1 cut(s) | 123 |
| MluCI | AATT | 3 cut(s) | 233, 458, 470 |
| MluNI | TGGCCA | 1 cut(s) | 123 |
| Mly113I | GGCGCC | 1 cut(s) | 454 |
| MlyI | GAGTC | 4 cut(s) | 212, 291, 448, 534 |
| MmeI | TCCRAC | 2 cut(s) | 247, 395 |
| MnlI | CCTC | 7 cut(s) | 33, 38, 98, 158, 269, 301, 320 |
| Mox20I | TGGCCA | 1 cut(s) | 123 |
| MscI | TGGCCA | 1 cut(s) | 123 |
| Msp20I | TGGCCA | 1 cut(s) | 123 |
| MspA1I | CMGCKG | 2 cut(s) | 210, 365 |
| MspI | CCGG | 3 cut(s) | 374, 451, 526 |
| MspR9I | CCNGG | 6 cut(s) | 13, 347, 374, 451, 483, 488 |
| MvaI | CCWGG | 4 cut(s) | 13, 347, 483, 488 |
| MvnI | CGCG | 2 cut(s) | 79, 137 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 13, 134, 417 |
| NarI | GGCGCC | 1 cut(s) | 454 |
| NciI | CCSGG | 2 cut(s) | 374, 451 |
| NdeII | GATC | 2 cut(s) | 53, 354 |
| NlaIV | GGNNCC | 2 cut(s) | 447, 455 |
| NmeAIII | GCCGAG | 1 cut(s) | 128 |
| NmuCI | GTSAC | 1 cut(s) | 196 |
| PceI | AGGCCT | 1 cut(s) | 311 |
| PfeI | GAWTC | 1 cut(s) | 507 |
| PkrI | GCNGC | 3 cut(s) | 6, 212, 410 |
| PleI | GAGTC | 4 cut(s) | 211, 290, 447, 534 |
| PluTI | GGCGCC | 1 cut(s) | 457 |
| PpsI | GAGTC | 4 cut(s) | 211, 290, 447, 534 |
| Psp6I | CCWGG | 4 cut(s) | 11, 345, 481, 486 |
| PspFI | CCCAGC | 1 cut(s) | 254 |
| PspGI | CCWGG | 4 cut(s) | 11, 345, 481, 486 |
| PspN4I | GGNNCC | 2 cut(s) | 447, 455 |
| PspPI | GGNCC | 3 cut(s) | 107, 238, 490 |
| PstNI | CAGNNNCTG | 1 cut(s) | 396 |
| PsuI | RGATCY | 1 cut(s) | 53 |
| SatI | GCNGC | 3 cut(s) | 5, 211, 409 |
| Sau3AI | GATC | 2 cut(s) | 53, 354 |
| Sau96I | GGNCC | 3 cut(s) | 107, 238, 490 |
| SchI | GAGTC | 4 cut(s) | 212, 291, 448, 534 |
| ScrFI | CCNGG | 6 cut(s) | 13, 347, 374, 451, 483, 488 |
| SduI | GDGCHC | 1 cut(s) | 254 |
| SetI | ASST | 5 cut(s) | 25, 43, 151, 227, 519 |
| SfaNI | GCATC | 2 cut(s) | 369, 395 |
| SfoI | GGCGCC | 1 cut(s) | 455 |
| SinI | GGWCC | 1 cut(s) | 238 |
| SmlI | CTYRAG | 1 cut(s) | 305 |
| SmoI | CTYRAG | 1 cut(s) | 305 |
| Sse9I | AATT | 3 cut(s) | 233, 458, 470 |
| SseBI | AGGCCT | 1 cut(s) | 311 |
| SspDI | GGCGCC | 1 cut(s) | 453 |
| SspMI | CTAG | 2 cut(s) | 226, 313 |
| StuI | AGGCCT | 1 cut(s) | 311 |
| StyD4I | CCNGG | 6 cut(s) | 11, 345, 372, 449, 481, 486 |
| StyI | CCWWGG | 1 cut(s) | 312 |
| TaaI | ACNGT | 1 cut(s) | 196 |
| TasI | AATT | 3 cut(s) | 233, 458, 470 |
| TauI | GCSGC | 1 cut(s) | 7 |
| TfiI | GAWTC | 1 cut(s) | 507 |
| TscAI | CASTG | 2 cut(s) | 295, 504 |
| TseFI | GTSAC | 1 cut(s) | 196 |
| TseI | GCWGC | 2 cut(s) | 210, 408 |
| Tsp45I | GTSAC | 1 cut(s) | 196 |
| TspGWI | ACGGA | 2 cut(s) | 215, 417 |
| TspRI | CASTG | 2 cut(s) | 295, 504 |
| VpaK11BI | GGWCC | 1 cut(s) | 238 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 343 |
| XmaJI | CCTAGG | 1 cut(s) | 312 |
| XspI | CTAG | 2 cut(s) | 226, 313 |