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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 514 |
| Acc36I | ACCTGC | 1 cut(s) | 514 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 43 |
| AccII | CGCG | 3 cut(s) | 9, 222, 486 |
| AciI | CCGC | 4 cut(s) | 222, 264, 315, 348 |
| AclWI | GGATC | 2 cut(s) | 335, 348 |
| AcsI | RAATTY | 1 cut(s) | 545 |
| AcuI | CTGAAG | 2 cut(s) | 72, 286 |
| AcyI | GRCGYC | 2 cut(s) | 236, 513 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 43, 111, 314, 523 |
| AgsI | TTSAA | 1 cut(s) | 418 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 83, 115 |
| AluBI | AGCT | 3 cut(s) | 34, 43, 448 |
| AluI | AGCT | 3 cut(s) | 34, 43, 448 |
| Alw21I | GWGCWC | 1 cut(s) | 412 |
| Alw26I | GTCTC | 1 cut(s) | 230 |
| Alw44I | GTGCAC | 1 cut(s) | 408 |
| AlwI | GGATC | 2 cut(s) | 335, 348 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 43 |
| ApaLI | GTGCAC | 1 cut(s) | 408 |
| ApoI | RAATTY | 1 cut(s) | 545 |
| AseI | ATTAAT | 2 cut(s) | 131, 384 |
| AspLEI | GCGC | 3 cut(s) | 11, 222, 498 |
| AspS9I | GGNCC | 1 cut(s) | 293 |
| AsuHPI | GGTGA | 1 cut(s) | 94 |
| AvaII | GGWCC | 1 cut(s) | 293 |
| BaeGI | GKGCMC | 1 cut(s) | 412 |
| BamHI | GGATCC | 1 cut(s) | 340 |
| BbsI | GAAGAC | 2 cut(s) | 92, 215 |
| Bbv12I | GWGCWC | 1 cut(s) | 412 |
| BccI | CCATC | 2 cut(s) | 352, 366 |
| BceAI | ACGGC | 2 cut(s) | 390, 419 |
| BclI | TGATCA | 1 cut(s) | 375 |
| BcoDI | GTCTC | 1 cut(s) | 230 |
| BfaI | CTAG | 1 cut(s) | 455 |
| BfuAI | ACCTGC | 1 cut(s) | 514 |
| Bme18I | GGWCC | 1 cut(s) | 293 |
| BmgT120I | GGNCC | 1 cut(s) | 293 |
| BmiI | GGNNCC | 1 cut(s) | 342 |
| BmsI | GCATC | 2 cut(s) | 34, 204 |
| BpiI | GAAGAC | 2 cut(s) | 92, 215 |
| BpmI | CTGGAG | 1 cut(s) | 20 |
| BsaHI | GRCGYC | 2 cut(s) | 236, 513 |
| BsaI | GGTCTC | 1 cut(s) | 230 |
| BsaWI | WCCGGW | 1 cut(s) | 424 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 43, 111, 314, 523 |
| BseGI | GGATG | 2 cut(s) | 344, 358 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 43, 111, 314, 523 |
| BseMII | CTCAG | 1 cut(s) | 318 |
| BseRI | GAGGAG | 1 cut(s) | 344 |
| BseSI | GKGCMC | 1 cut(s) | 412 |
| BseYI | CCCAGC | 1 cut(s) | 43 |
| Bsh1236I | CGCG | 3 cut(s) | 9, 222, 486 |
| BsiHKAI | GWGCWC | 1 cut(s) | 412 |
| BsiSI | CCGG | 1 cut(s) | 425 |
| BslFI | GGGAC | 2 cut(s) | 49, 61 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 43, 111, 314, 523 |
| BsmAI | GTCTC | 1 cut(s) | 230 |
| BsmFI | GGGAC | 2 cut(s) | 49, 61 |
| Bso31I | GGTCTC | 1 cut(s) | 230 |
| Bsp1286I | GDGCHC | 1 cut(s) | 412 |
| Bsp143I | GATC | 2 cut(s) | 340, 375 |
| BspACI | CCGC | 4 cut(s) | 222, 264, 315, 348 |
| BspCNI | CTCAG | 1 cut(s) | 319 |
| BspFNI | CGCG | 3 cut(s) | 9, 222, 486 |
| BspLI | GGNNCC | 1 cut(s) | 342 |
| BspMI | ACCTGC | 1 cut(s) | 514 |
| BspPI | GGATC | 2 cut(s) | 335, 348 |
| BspTNI | GGTCTC | 1 cut(s) | 230 |
| BssMI | GATC | 2 cut(s) | 340, 375 |
| BssNI | GRCGYC | 2 cut(s) | 236, 513 |
| Bst6I | CTCTTC | 2 cut(s) | 126, 198 |
| BstACI | GRCGYC | 2 cut(s) | 236, 513 |
| BstC8I | GCNNGC | 1 cut(s) | 450 |
| BstDEI | CTNAG | 2 cut(s) | 190, 327 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 109 |
| BstF5I | GGATG | 2 cut(s) | 344, 358 |
| BstFNI | CGCG | 3 cut(s) | 9, 222, 486 |
| BstHHI | GCGC | 3 cut(s) | 11, 222, 498 |
| BstKTI | GATC | 2 cut(s) | 343, 378 |
| BstMAI | GTCTC | 1 cut(s) | 230 |
| BstMBI | GATC | 2 cut(s) | 340, 375 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 31, 40 |
| BstSLI | GKGCMC | 1 cut(s) | 412 |
| BstUI | CGCG | 3 cut(s) | 9, 222, 486 |
| BstV2I | GAAGAC | 2 cut(s) | 92, 215 |
| BstX2I | RGATCY | 1 cut(s) | 340 |
| BstYI | RGATCY | 1 cut(s) | 340 |
| BtsCI | GGATG | 2 cut(s) | 344, 358 |
| BtsIMutI | CAGTG | 1 cut(s) | 324 |
| BveI | ACCTGC | 1 cut(s) | 514 |
| Cac8I | GCNNGC | 1 cut(s) | 450 |
| CaiI | CAGNNNCTG | 1 cut(s) | 43 |
| CfoI | GCGC | 3 cut(s) | 11, 222, 498 |
| Cfr13I | GGNCC | 1 cut(s) | 293 |
| CseI | GACGC | 2 cut(s) | 244, 521 |
| CviAII | CATG | 1 cut(s) | 79 |
| CviJI | RGCY | 5 cut(s) | 34, 43, 270, 405, 448 |
| CviKI_1 | RGCY | 5 cut(s) | 34, 43, 270, 405, 448 |
| DdeI | CTNAG | 2 cut(s) | 190, 327 |
| DpnI | GATC | 2 cut(s) | 342, 377 |
| DpnII | GATC | 2 cut(s) | 340, 375 |
| Eam1104I | CTCTTC | 2 cut(s) | 126, 198 |
| EarI | CTCTTC | 2 cut(s) | 126, 198 |
| Eco31I | GGTCTC | 1 cut(s) | 230 |
| Eco47I | GGWCC | 1 cut(s) | 293 |
| Eco57I | CTGAAG | 2 cut(s) | 72, 286 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 109 |
| FaeI | CATG | 1 cut(s) | 82 |
| FaiI | YATR | 7 cut(s) | 58, 80, 108, 173, 245, 440, 444 |
| FaqI | GGGAC | 2 cut(s) | 49, 61 |
| FatI | CATG | 1 cut(s) | 78 |
| FauI | CCCGC | 1 cut(s) | 308 |
| FbaI | TGATCA | 1 cut(s) | 375 |
| FokI | GGATG | 2 cut(s) | 331, 345 |
| FspBI | CTAG | 1 cut(s) | 455 |
| GlaI | GCGC | 3 cut(s) | 10, 221, 497 |
| GsaI | CCCAGC | 1 cut(s) | 47 |
| GsuI | CTGGAG | 1 cut(s) | 20 |
| HapII | CCGG | 1 cut(s) | 425 |
| HgaI | GACGC | 2 cut(s) | 244, 521 |
| HhaI | GCGC | 3 cut(s) | 11, 222, 498 |
| Hin1I | GRCGYC | 2 cut(s) | 236, 513 |
| Hin1II | CATG | 1 cut(s) | 82 |
| Hin6I | GCGC | 3 cut(s) | 9, 220, 496 |
| HinP1I | GCGC | 3 cut(s) | 9, 220, 496 |
| HincII | GTYRAC | 1 cut(s) | 169 |
| HindII | GTYRAC | 1 cut(s) | 169 |
| HpaII | CCGG | 1 cut(s) | 425 |
| HphI | GGTGA | 1 cut(s) | 94 |
| Hpy166II | GTNNAC | 3 cut(s) | 169, 296, 410 |
| Hpy188I | TCNGA | 3 cut(s) | 30, 234, 305 |
| Hpy188III | TCNNGA | 1 cut(s) | 37 |
| Hpy8I | GTNNAC | 3 cut(s) | 169, 296, 410 |
| HpyAV | CCTTC | 2 cut(s) | 106, 310 |
| HpyCH4V | TGCA | 1 cut(s) | 410 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 31, 40 |
| HpyF3I | CTNAG | 2 cut(s) | 190, 327 |
| Hsp92I | GRCGYC | 2 cut(s) | 236, 513 |
| Hsp92II | CATG | 1 cut(s) | 82 |
| HspAI | GCGC | 3 cut(s) | 9, 220, 496 |
| Ksp22I | TGATCA | 1 cut(s) | 375 |
| Kzo9I | GATC | 2 cut(s) | 340, 375 |
| LmnI | GCTCC | 3 cut(s) | 39, 331, 493 |
| LpnPI | CCDG | 9 cut(s) | 29, 50, 230, 243, 321, 434, 438, 476, 509 |
| LweI | GCATC | 2 cut(s) | 34, 204 |
| MaeI | CTAG | 1 cut(s) | 455 |
| MaeIII | GTNAC | 1 cut(s) | 529 |
| MalI | GATC | 2 cut(s) | 342, 377 |
| MboI | GATC | 2 cut(s) | 340, 375 |
| MboII | GAAGA | 7 cut(s) | 91, 94, 97, 113, 212, 215, 220 |
| MflI | RGATCY | 1 cut(s) | 340 |
| MhlI | GDGCHC | 1 cut(s) | 412 |
| MluCI | AATT | 2 cut(s) | 132, 545 |
| MnlI | CCTC | 5 cut(s) | 240, 250, 260, 322, 473 |
| MseI | TTAA | 4 cut(s) | 131, 143, 384, 396 |
| MslI | CAYNNNNRTG | 1 cut(s) | 536 |
| MspI | CCGG | 1 cut(s) | 425 |
| MvnI | CGCG | 3 cut(s) | 9, 222, 486 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 31, 40 |
| NdeII | GATC | 2 cut(s) | 340, 375 |
| NlaIII | CATG | 1 cut(s) | 82 |
| NlaIV | GGNNCC | 1 cut(s) | 342 |
| NmuCI | GTSAC | 1 cut(s) | 529 |
| PaqCI | CACCTGC | 1 cut(s) | 514 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 43 |
| PshBI | ATTAAT | 2 cut(s) | 131, 384 |
| PspFI | CCCAGC | 1 cut(s) | 43 |
| PspN4I | GGNNCC | 1 cut(s) | 342 |
| PspPI | GGNCC | 1 cut(s) | 293 |
| PstNI | CAGNNNCTG | 1 cut(s) | 43 |
| PsuI | RGATCY | 1 cut(s) | 340 |
| RseI | CAYNNNNRTG | 1 cut(s) | 536 |
| SaqAI | TTAA | 4 cut(s) | 131, 143, 384, 396 |
| Sau3AI | GATC | 2 cut(s) | 340, 375 |
| Sau96I | GGNCC | 1 cut(s) | 293 |
| SduI | GDGCHC | 1 cut(s) | 412 |
| SfaNI | GCATC | 2 cut(s) | 34, 204 |
| SinI | GGWCC | 1 cut(s) | 293 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 536 |
| Sse9I | AATT | 2 cut(s) | 132, 545 |
| SsiI | CCGC | 4 cut(s) | 222, 264, 315, 348 |
| SspMI | CTAG | 1 cut(s) | 455 |
| TaqI | TCGA | 3 cut(s) | 72, 198, 549 |
| TaqII | GACCGA | 1 cut(s) | 227 |
| TasI | AATT | 2 cut(s) | 132, 545 |
| Tru1I | TTAA | 4 cut(s) | 131, 143, 384, 396 |
| Tru9I | TTAA | 4 cut(s) | 131, 143, 384, 396 |
| TscAI | CASTG | 1 cut(s) | 331 |
| TseFI | GTSAC | 1 cut(s) | 529 |
| Tsp45I | GTSAC | 1 cut(s) | 529 |
| TspDTI | ATGAA | 1 cut(s) | 95 |
| TspGWI | ACGGA | 1 cut(s) | 353 |
| TspRI | CASTG | 1 cut(s) | 331 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 43 |
| VneI | GTGCAC | 1 cut(s) | 408 |
| VpaK11BI | GGWCC | 1 cut(s) | 293 |
| VspI | ATTAAT | 2 cut(s) | 131, 384 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 109 |
| XapI | RAATTY | 1 cut(s) | 545 |
| XspI | CTAG | 1 cut(s) | 455 |