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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 298 |
| Acc36I | ACCTGC | 1 cut(s) | 298 |
| AccII | CGCG | 3 cut(s) | 93, 270, 317 |
| AciI | CCGC | 7 cut(s) | 8, 31, 41, 77, 91, 219, 315 |
| AclI | AACGTT | 1 cut(s) | 84 |
| AclWI | GGATC | 1 cut(s) | 41 |
| AcoI | YGGCCR | 1 cut(s) | 312 |
| AfaI | GTAC | 1 cut(s) | 369 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 253, 307, 410 |
| AgsI | TTSAA | 2 cut(s) | 103, 334 |
| AjnI | CCWGG | 1 cut(s) | 202 |
| AluBI | AGCT | 1 cut(s) | 232 |
| AluI | AGCT | 1 cut(s) | 232 |
| Alw26I | GTCTC | 1 cut(s) | 169 |
| AlwI | GGATC | 1 cut(s) | 41 |
| AoxI | GGCC | 4 cut(s) | 135, 205, 297, 312 |
| AspLEI | GCGC | 4 cut(s) | 95, 270, 319, 352 |
| AspS9I | GGNCC | 1 cut(s) | 298 |
| AsuHPI | GGTGA | 1 cut(s) | 208 |
| AxyI | CCTNAGG | 1 cut(s) | 132 |
| BbvCI | CCTCAGC | 1 cut(s) | 294 |
| BciT130I | CCWGG | 1 cut(s) | 204 |
| BcoDI | GTCTC | 1 cut(s) | 169 |
| BfaI | CTAG | 2 cut(s) | 120, 393 |
| BfoI | RGCGCY | 1 cut(s) | 353 |
| BfuAI | ACCTGC | 1 cut(s) | 298 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 204 |
| BisI | GCNGC | 1 cut(s) | 315 |
| BlsI | GCNGC | 1 cut(s) | 316 |
| Bme1390I | CCNGG | 1 cut(s) | 204 |
| BmgT120I | GGNCC | 1 cut(s) | 298 |
| BmiI | GGNNCC | 2 cut(s) | 129, 398 |
| BmrFI | CCNGG | 1 cut(s) | 204 |
| Bpu10I | CCTNAGC | 1 cut(s) | 294 |
| BsaJI | CCNNGG | 1 cut(s) | 20 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 253, 307, 410 |
| Bse21I | CCTNAGG | 1 cut(s) | 132 |
| BseBI | CCWGG | 1 cut(s) | 204 |
| BseDI | CCNNGG | 1 cut(s) | 20 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 253, 307, 410 |
| BseMII | CTCAG | 3 cut(s) | 146, 180, 285 |
| BseRI | GAGGAG | 1 cut(s) | 37 |
| BseYI | CCCAGC | 2 cut(s) | 73, 352 |
| Bsh1236I | CGCG | 3 cut(s) | 93, 270, 317 |
| BshFI | GGCC | 4 cut(s) | 137, 207, 299, 314 |
| BsiSI | CCGG | 1 cut(s) | 411 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 253, 307, 410 |
| BsmAI | GTCTC | 1 cut(s) | 169 |
| BsnI | GGCC | 4 cut(s) | 137, 207, 299, 314 |
| Bsp143I | GATC | 1 cut(s) | 33 |
| BspACI | CCGC | 7 cut(s) | 8, 31, 41, 77, 91, 219, 315 |
| BspANI | GGCC | 4 cut(s) | 137, 207, 299, 314 |
| BspCNI | CTCAG | 3 cut(s) | 145, 179, 286 |
| BspFNI | CGCG | 3 cut(s) | 93, 270, 317 |
| BspLI | GGNNCC | 2 cut(s) | 129, 398 |
| BspMI | ACCTGC | 1 cut(s) | 298 |
| BspPI | GGATC | 1 cut(s) | 41 |
| BssECI | CCNNGG | 1 cut(s) | 20 |
| BssMI | GATC | 1 cut(s) | 33 |
| Bst2UI | CCWGG | 1 cut(s) | 204 |
| BstC8I | GCNNGC | 3 cut(s) | 29, 234, 377 |
| BstDEI | CTNAG | 3 cut(s) | 132, 166, 294 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 251 |
| BstFNI | CGCG | 3 cut(s) | 93, 270, 317 |
| BstH2I | RGCGCY | 1 cut(s) | 353 |
| BstHHI | GCGC | 4 cut(s) | 95, 270, 319, 352 |
| BstKTI | GATC | 1 cut(s) | 36 |
| BstMAI | GTCTC | 1 cut(s) | 169 |
| BstMBI | GATC | 1 cut(s) | 33 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 204, 356, 396 |
| BstNI | CCWGG | 1 cut(s) | 204 |
| BstSCI | CCNGG | 1 cut(s) | 202 |
| BstUI | CGCG | 3 cut(s) | 93, 270, 317 |
| Bsu36I | CCTNAGG | 1 cut(s) | 132 |
| BsuRI | GGCC | 4 cut(s) | 137, 207, 299, 314 |
| BtgZI | GCGATG | 1 cut(s) | 179 |
| BtsIMutI | CAGTG | 1 cut(s) | 174 |
| BveI | ACCTGC | 1 cut(s) | 298 |
| Cac8I | GCNNGC | 3 cut(s) | 29, 234, 377 |
| CfoI | GCGC | 4 cut(s) | 95, 270, 319, 352 |
| Cfr13I | GGNCC | 1 cut(s) | 298 |
| Csp6I | GTAC | 1 cut(s) | 368 |
| CviQI | GTAC | 1 cut(s) | 368 |
| DdeI | CTNAG | 3 cut(s) | 132, 166, 294 |
| DpnI | GATC | 1 cut(s) | 35 |
| DpnII | GATC | 1 cut(s) | 33 |
| EaeI | YGGCCR | 1 cut(s) | 312 |
| EciI | GGCGGA | 1 cut(s) | 30 |
| Eco81I | CCTNAGG | 1 cut(s) | 132 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 251 |
| EcoRII | CCWGG | 1 cut(s) | 202 |
| FaiI | YATR | 2 cut(s) | 224, 228 |
| Fnu4HI | GCNGC | 1 cut(s) | 315 |
| Fsp4HI | GCNGC | 1 cut(s) | 315 |
| FspBI | CTAG | 2 cut(s) | 120, 393 |
| GlaI | GCGC | 4 cut(s) | 94, 269, 318, 351 |
| GluI | GCNGC | 1 cut(s) | 315 |
| GsaI | CCCAGC | 2 cut(s) | 77, 356 |
| HaeII | RGCGCY | 1 cut(s) | 353 |
| HaeIII | GGCC | 4 cut(s) | 137, 207, 299, 314 |
| HapII | CCGG | 1 cut(s) | 411 |
| HhaI | GCGC | 4 cut(s) | 95, 270, 319, 352 |
| Hin6I | GCGC | 4 cut(s) | 93, 268, 317, 350 |
| HinP1I | GCGC | 4 cut(s) | 93, 268, 317, 350 |
| HincII | GTYRAC | 3 cut(s) | 70, 82, 88 |
| HindII | GTYRAC | 3 cut(s) | 70, 82, 88 |
| HpaI | GTTAAC | 3 cut(s) | 70, 82, 88 |
| HpaII | CCGG | 1 cut(s) | 411 |
| HphI | GGTGA | 1 cut(s) | 208 |
| Hpy166II | GTNNAC | 3 cut(s) | 70, 82, 88 |
| Hpy8I | GTNNAC | 3 cut(s) | 70, 82, 88 |
| HpyCH4IV | ACGT | 1 cut(s) | 84 |
| HpyCH4V | TGCA | 1 cut(s) | 282 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 204, 356, 396 |
| HpyF3I | CTNAG | 3 cut(s) | 132, 166, 294 |
| HpySE526I | ACGT | 1 cut(s) | 84 |
| HspAI | GCGC | 4 cut(s) | 93, 268, 317, 350 |
| KspAI | GTTAAC | 3 cut(s) | 70, 82, 88 |
| Kzo9I | GATC | 1 cut(s) | 33 |
| LmnI | GCTCC | 4 cut(s) | 24, 127, 265, 396 |
| MaeI | CTAG | 2 cut(s) | 120, 393 |
| MaeII | ACGT | 1 cut(s) | 84 |
| MalI | GATC | 1 cut(s) | 35 |
| MboI | GATC | 1 cut(s) | 33 |
| MmeI | TCCRAC | 1 cut(s) | 175 |
| MnlI | CCTC | 6 cut(s) | 15, 141, 166, 224, 257, 289 |
| MseI | TTAA | 3 cut(s) | 69, 81, 87 |
| MslI | CAYNNNNRTG | 1 cut(s) | 221 |
| MspA1I | CMGCKG | 1 cut(s) | 77 |
| MspI | CCGG | 1 cut(s) | 411 |
| MspR9I | CCNGG | 1 cut(s) | 204 |
| MvaI | CCWGG | 1 cut(s) | 204 |
| MvnI | CGCG | 3 cut(s) | 93, 270, 317 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 204, 356, 396 |
| NdeII | GATC | 1 cut(s) | 33 |
| NlaIV | GGNNCC | 2 cut(s) | 129, 398 |
| NmeAIII | GCCGAG | 1 cut(s) | 45 |
| PaqCI | CACCTGC | 1 cut(s) | 298 |
| PkrI | GCNGC | 1 cut(s) | 316 |
| Psp1406I | AACGTT | 1 cut(s) | 84 |
| Psp6I | CCWGG | 1 cut(s) | 202 |
| PspFI | CCCAGC | 2 cut(s) | 73, 352 |
| PspGI | CCWGG | 1 cut(s) | 202 |
| PspN4I | GGNNCC | 2 cut(s) | 129, 398 |
| PspPI | GGNCC | 1 cut(s) | 298 |
| RsaI | GTAC | 1 cut(s) | 369 |
| RsaNI | GTAC | 1 cut(s) | 368 |
| RseI | CAYNNNNRTG | 1 cut(s) | 221 |
| SaqAI | TTAA | 3 cut(s) | 69, 81, 87 |
| SatI | GCNGC | 1 cut(s) | 315 |
| Sau3AI | GATC | 1 cut(s) | 33 |
| Sau96I | GGNCC | 1 cut(s) | 298 |
| ScrFI | CCNGG | 1 cut(s) | 204 |
| SetI | ASST | 6 cut(s) | 87, 216, 234, 249, 312, 433 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 221 |
| SsiI | CCGC | 7 cut(s) | 8, 31, 41, 77, 91, 219, 315 |
| SspMI | CTAG | 2 cut(s) | 120, 393 |
| StyD4I | CCNGG | 1 cut(s) | 202 |
| TaiI | ACGT | 1 cut(s) | 87 |
| TaqI | TCGA | 1 cut(s) | 326 |
| TatI | WGTACW | 1 cut(s) | 367 |
| TauI | GCSGC | 1 cut(s) | 317 |
| Tru1I | TTAA | 3 cut(s) | 69, 81, 87 |
| Tru9I | TTAA | 3 cut(s) | 69, 81, 87 |
| TscAI | CASTG | 1 cut(s) | 174 |
| TspRI | CASTG | 1 cut(s) | 174 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 251 |
| XspI | CTAG | 2 cut(s) | 120, 393 |