No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 274 |
| AccII | CGCG | 1 cut(s) | 36 |
| AciI | CCGC | 3 cut(s) | 34, 114, 179 |
| AclI | AACGTT | 1 cut(s) | 27 |
| AclWI | GGATC | 1 cut(s) | 156 |
| AcoI | YGGCCR | 1 cut(s) | 237 |
| AfaI | GTAC | 1 cut(s) | 164 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 67, 190 |
| AgsI | TTSAA | 1 cut(s) | 46 |
| AlwI | GGATC | 1 cut(s) | 156 |
| AoxI | GGCC | 3 cut(s) | 222, 237, 282 |
| AspLEI | GCGC | 1 cut(s) | 38 |
| AspS9I | GGNCC | 3 cut(s) | 91, 181, 223 |
| AsuC2I | CCSGG | 2 cut(s) | 89, 296 |
| AsuHPI | GGTGA | 2 cut(s) | 77, 126 |
| AvaII | GGWCC | 2 cut(s) | 91, 181 |
| BalI | TGGCCA | 1 cut(s) | 239 |
| BanI | GGYRCC | 1 cut(s) | 274 |
| BcnI | CCSGG | 2 cut(s) | 89, 296 |
| BfaI | CTAG | 1 cut(s) | 63 |
| Bme1390I | CCNGG | 2 cut(s) | 89, 296 |
| Bme18I | GGWCC | 2 cut(s) | 91, 181 |
| BmgT120I | GGNCC | 3 cut(s) | 91, 181, 223 |
| BmiI | GGNNCC | 4 cut(s) | 72, 92, 93, 276 |
| BmrFI | CCNGG | 2 cut(s) | 89, 296 |
| BpuMI | CCSGG | 2 cut(s) | 89, 296 |
| BsaJI | CCNNGG | 1 cut(s) | 219 |
| BsaWI | WCCGGW | 1 cut(s) | 277 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 67, 190 |
| Bse118I | RCCGGY | 1 cut(s) | 169 |
| BseDI | CCNNGG | 1 cut(s) | 219 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 67, 190 |
| Bsh1236I | CGCG | 1 cut(s) | 36 |
| BshFI | GGCC | 3 cut(s) | 224, 239, 284 |
| BshNI | GGYRCC | 1 cut(s) | 274 |
| BsiSI | CCGG | 5 cut(s) | 88, 146, 170, 278, 296 |
| BslFI | GGGAC | 1 cut(s) | 77 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 67, 190 |
| BsmFI | GGGAC | 1 cut(s) | 77 |
| BsnI | GGCC | 3 cut(s) | 224, 239, 284 |
| Bsp143I | GATC | 2 cut(s) | 54, 148 |
| BspACI | CCGC | 3 cut(s) | 34, 114, 179 |
| BspANI | GGCC | 3 cut(s) | 224, 239, 284 |
| BspFNI | CGCG | 1 cut(s) | 36 |
| BspLI | GGNNCC | 4 cut(s) | 72, 92, 93, 276 |
| BspPI | GGATC | 1 cut(s) | 156 |
| BspT107I | GGYRCC | 1 cut(s) | 274 |
| BsrFI | RCCGGY | 1 cut(s) | 169 |
| BssAI | RCCGGY | 1 cut(s) | 169 |
| BssECI | CCNNGG | 1 cut(s) | 219 |
| BssMI | GATC | 2 cut(s) | 54, 148 |
| BstC8I | GCNNGC | 1 cut(s) | 171 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 188 |
| BstFNI | CGCG | 1 cut(s) | 36 |
| BstHHI | GCGC | 1 cut(s) | 38 |
| BstKTI | GATC | 2 cut(s) | 57, 151 |
| BstMBI | GATC | 2 cut(s) | 54, 148 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 281, 296 |
| BstSCI | CCNGG | 2 cut(s) | 87, 294 |
| BstUI | CGCG | 1 cut(s) | 36 |
| BstX2I | RGATCY | 1 cut(s) | 148 |
| BstYI | RGATCY | 1 cut(s) | 148 |
| BsuRI | GGCC | 3 cut(s) | 224, 239, 284 |
| Cac8I | GCNNGC | 1 cut(s) | 171 |
| CfoI | GCGC | 1 cut(s) | 38 |
| Cfr10I | RCCGGY | 1 cut(s) | 169 |
| Cfr13I | GGNCC | 3 cut(s) | 91, 181, 223 |
| Csp6I | GTAC | 1 cut(s) | 163 |
| CviAII | CATG | 1 cut(s) | 142 |
| CviJI | RGCY | 5 cut(s) | 169, 218, 224, 239, 284 |
| CviKI_1 | RGCY | 5 cut(s) | 169, 218, 224, 239, 284 |
| CviQI | GTAC | 1 cut(s) | 163 |
| DpnI | GATC | 2 cut(s) | 56, 150 |
| DpnII | GATC | 2 cut(s) | 54, 148 |
| EaeI | YGGCCR | 1 cut(s) | 237 |
| Eco47I | GGWCC | 2 cut(s) | 91, 181 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 188 |
| EcoO109I | RGGNCCY | 1 cut(s) | 91 |
| FaeI | CATG | 1 cut(s) | 145 |
| FaiI | YATR | 2 cut(s) | 143, 161 |
| FaqI | GGGAC | 1 cut(s) | 77 |
| FatI | CATG | 1 cut(s) | 141 |
| FspBI | CTAG | 1 cut(s) | 63 |
| GlaI | GCGC | 1 cut(s) | 37 |
| HaeIII | GGCC | 3 cut(s) | 224, 239, 284 |
| HapII | CCGG | 5 cut(s) | 88, 146, 170, 278, 296 |
| HhaI | GCGC | 1 cut(s) | 38 |
| Hin1II | CATG | 1 cut(s) | 145 |
| Hin6I | GCGC | 1 cut(s) | 36 |
| HinP1I | GCGC | 1 cut(s) | 36 |
| HincII | GTYRAC | 1 cut(s) | 31 |
| HindII | GTYRAC | 1 cut(s) | 31 |
| HinfI | GANTC | 3 cut(s) | 100, 117, 255 |
| HpaI | GTTAAC | 1 cut(s) | 31 |
| HpaII | CCGG | 5 cut(s) | 88, 146, 170, 278, 296 |
| HphI | GGTGA | 2 cut(s) | 77, 126 |
| Hpy166II | GTNNAC | 1 cut(s) | 31 |
| Hpy188I | TCNGA | 1 cut(s) | 54 |
| Hpy188III | TCNNGA | 1 cut(s) | 252 |
| Hpy8I | GTNNAC | 1 cut(s) | 31 |
| Hpy99I | CGWCG | 1 cut(s) | 263 |
| HpyAV | CCTTC | 1 cut(s) | 265 |
| HpyCH4IV | ACGT | 1 cut(s) | 27 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 281, 296 |
| HpySE526I | ACGT | 1 cut(s) | 27 |
| Hsp92II | CATG | 1 cut(s) | 145 |
| HspAI | GCGC | 1 cut(s) | 36 |
| KflI | GGGWCCC | 1 cut(s) | 91 |
| KroI | GCCGGC | 1 cut(s) | 169 |
| KroNI | GCCGGC | 1 cut(s) | 171 |
| KspAI | GTTAAC | 1 cut(s) | 31 |
| Kzo9I | GATC | 2 cut(s) | 54, 148 |
| LpnPI | CCDG | 5 cut(s) | 101, 159, 183, 193, 291 |
| MaeI | CTAG | 1 cut(s) | 63 |
| MaeII | ACGT | 1 cut(s) | 27 |
| MaeIII | GTNAC | 2 cut(s) | 23, 132 |
| MalI | GATC | 2 cut(s) | 56, 150 |
| MboI | GATC | 2 cut(s) | 54, 148 |
| MboII | GAAGA | 1 cut(s) | 280 |
| MflI | RGATCY | 1 cut(s) | 148 |
| MlsI | TGGCCA | 1 cut(s) | 239 |
| MluNI | TGGCCA | 1 cut(s) | 239 |
| MlyI | GAGTC | 2 cut(s) | 94, 126 |
| MnlI | CCTC | 3 cut(s) | 194, 214, 274 |
| Mox20I | TGGCCA | 1 cut(s) | 239 |
| MroNI | GCCGGC | 1 cut(s) | 169 |
| MscI | TGGCCA | 1 cut(s) | 239 |
| MseI | TTAA | 2 cut(s) | 12, 30 |
| MslI | CAYNNNNRTG | 1 cut(s) | 245 |
| Msp20I | TGGCCA | 1 cut(s) | 239 |
| MspI | CCGG | 5 cut(s) | 88, 146, 170, 278, 296 |
| MspR9I | CCNGG | 2 cut(s) | 89, 296 |
| MvnI | CGCG | 1 cut(s) | 36 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 281, 296 |
| NaeI | GCCGGC | 1 cut(s) | 171 |
| NciI | CCSGG | 2 cut(s) | 89, 296 |
| NdeII | GATC | 2 cut(s) | 54, 148 |
| NgoMIV | GCCGGC | 1 cut(s) | 169 |
| NlaIII | CATG | 1 cut(s) | 145 |
| NlaIV | GGNNCC | 4 cut(s) | 72, 92, 93, 276 |
| NmeAIII | GCCGAG | 1 cut(s) | 244 |
| NmuCI | GTSAC | 1 cut(s) | 132 |
| PdiI | GCCGGC | 1 cut(s) | 171 |
| PfeI | GAWTC | 1 cut(s) | 255 |
| PleI | GAGTC | 2 cut(s) | 94, 125 |
| PpsI | GAGTC | 2 cut(s) | 94, 125 |
| PpuMI | RGGWCCY | 1 cut(s) | 91 |
| Psp1406I | AACGTT | 1 cut(s) | 27 |
| Psp5II | RGGWCCY | 1 cut(s) | 91 |
| PspN4I | GGNNCC | 4 cut(s) | 72, 92, 93, 276 |
| PspPI | GGNCC | 3 cut(s) | 91, 181, 223 |
| PspPPI | RGGWCCY | 1 cut(s) | 91 |
| PsuI | RGATCY | 1 cut(s) | 148 |
| RsaI | GTAC | 1 cut(s) | 164 |
| RsaNI | GTAC | 1 cut(s) | 163 |
| RseI | CAYNNNNRTG | 1 cut(s) | 245 |
| SaqAI | TTAA | 2 cut(s) | 12, 30 |
| Sau3AI | GATC | 2 cut(s) | 54, 148 |
| Sau96I | GGNCC | 3 cut(s) | 91, 181, 223 |
| SchI | GAGTC | 2 cut(s) | 94, 126 |
| ScrFI | CCNGG | 2 cut(s) | 89, 296 |
| SetI | ASST | 2 cut(s) | 30, 186 |
| SinI | GGWCC | 2 cut(s) | 91, 181 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 245 |
| SsiI | CCGC | 3 cut(s) | 34, 114, 179 |
| SspMI | CTAG | 1 cut(s) | 63 |
| StyD4I | CCNGG | 2 cut(s) | 87, 294 |
| TaiI | ACGT | 1 cut(s) | 30 |
| TaqI | TCGA | 1 cut(s) | 258 |
| TfiI | GAWTC | 1 cut(s) | 255 |
| Tru1I | TTAA | 2 cut(s) | 12, 30 |
| Tru9I | TTAA | 2 cut(s) | 12, 30 |
| TseFI | GTSAC | 1 cut(s) | 132 |
| Tsp45I | GTSAC | 1 cut(s) | 132 |
| VpaK11BI | GGWCC | 2 cut(s) | 91, 181 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 188 |
| XspI | CTAG | 1 cut(s) | 63 |