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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 74, 334 |
| AciI | CCGC | 7 cut(s) | 38, 72, 74, 116, 162, 185, 365 |
| AclWI | GGATC | 2 cut(s) | 48, 162 |
| AcoI | YGGCCR | 2 cut(s) | 75, 255 |
| AcsI | RAATTY | 1 cut(s) | 272 |
| AcuI | CTGAAG | 1 cut(s) | 305 |
| AfaI | GTAC | 1 cut(s) | 224 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 196, 361 |
| AjnI | CCWGG | 1 cut(s) | 127 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 278, 310 |
| AluBI | AGCT | 2 cut(s) | 92, 175 |
| AluI | AGCT | 2 cut(s) | 92, 175 |
| Alw26I | GTCTC | 1 cut(s) | 100 |
| AlwI | GGATC | 2 cut(s) | 48, 162 |
| AoxI | GGCC | 4 cut(s) | 75, 120, 240, 255 |
| ApeKI | GCWGC | 1 cut(s) | 132 |
| ApoI | RAATTY | 1 cut(s) | 272 |
| AspS9I | GGNCC | 3 cut(s) | 121, 187, 241 |
| AsuC2I | CCSGG | 1 cut(s) | 147 |
| AvaII | GGWCC | 1 cut(s) | 187 |
| BalI | TGGCCA | 1 cut(s) | 257 |
| BbvI | GCAGC | 1 cut(s) | 144 |
| BccI | CCATC | 2 cut(s) | 66, 322 |
| BciT130I | CCWGG | 1 cut(s) | 129 |
| BcnI | CCSGG | 1 cut(s) | 147 |
| BcoDI | GTCTC | 1 cut(s) | 100 |
| BfaI | CTAG | 1 cut(s) | 342 |
| BisI | GCNGC | 3 cut(s) | 75, 133, 162 |
| BlsI | GCNGC | 3 cut(s) | 76, 134, 163 |
| Bme1390I | CCNGG | 2 cut(s) | 129, 147 |
| Bme18I | GGWCC | 1 cut(s) | 187 |
| BmgT120I | GGNCC | 3 cut(s) | 121, 187, 241 |
| BmrFI | CCNGG | 2 cut(s) | 129, 147 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 147 |
| BsaJI | CCNNGG | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 196, 361 |
| Bse118I | RCCGGY | 1 cut(s) | 361 |
| Bse1I | ACTGG | 1 cut(s) | 186 |
| BseBI | CCWGG | 1 cut(s) | 129 |
| BseDI | CCNNGG | 1 cut(s) | 72 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 196, 361 |
| BseNI | ACTGG | 1 cut(s) | 186 |
| BseXI | GCAGC | 1 cut(s) | 144 |
| BseYI | CCCAGC | 1 cut(s) | 88 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 334 |
| BshFI | GGCC | 4 cut(s) | 77, 122, 242, 257 |
| BsiSI | CCGG | 2 cut(s) | 146, 362 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 196, 361 |
| BsmAI | GTCTC | 1 cut(s) | 100 |
| BsnI | GGCC | 4 cut(s) | 77, 122, 242, 257 |
| Bsp143I | GATC | 2 cut(s) | 40, 154 |
| BspACI | CCGC | 7 cut(s) | 38, 72, 74, 116, 162, 185, 365 |
| BspANI | GGCC | 4 cut(s) | 77, 122, 242, 257 |
| BspFNI | CGCG | 2 cut(s) | 74, 334 |
| BspPI | GGATC | 2 cut(s) | 48, 162 |
| BsrFI | RCCGGY | 1 cut(s) | 361 |
| BsrI | ACTGG | 1 cut(s) | 186 |
| BssAI | RCCGGY | 1 cut(s) | 361 |
| BssECI | CCNNGG | 1 cut(s) | 72 |
| BssMI | GATC | 2 cut(s) | 40, 154 |
| Bst2UI | CCWGG | 1 cut(s) | 129 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 79, 177, 215, 326 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 194 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 2 cut(s) | 74, 334 |
| BstKTI | GATC | 2 cut(s) | 43, 157 |
| BstMAI | GTCTC | 1 cut(s) | 100 |
| BstMBI | GATC | 2 cut(s) | 40, 154 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 138, 299, 325 |
| BstNI | CCWGG | 1 cut(s) | 129 |
| BstSCI | CCNGG | 2 cut(s) | 127, 145 |
| BstUI | CGCG | 2 cut(s) | 74, 334 |
| BstV1I | GCAGC | 1 cut(s) | 144 |
| BstX2I | RGATCY | 1 cut(s) | 154 |
| BstYI | RGATCY | 1 cut(s) | 154 |
| BsuRI | GGCC | 4 cut(s) | 77, 122, 242, 257 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtgZI | GCGATG | 1 cut(s) | 300 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 1 cut(s) | 179 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 79, 177, 215, 326 |
| Cfr10I | RCCGGY | 1 cut(s) | 361 |
| Cfr13I | GGNCC | 3 cut(s) | 121, 187, 241 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| Csp6I | GTAC | 1 cut(s) | 223 |
| CviAII | CATG | 1 cut(s) | 259 |
| CviJI | RGCY | 6 cut(s) | 77, 92, 122, 175, 242, 257 |
| CviKI_1 | RGCY | 6 cut(s) | 77, 92, 122, 175, 242, 257 |
| CviQI | GTAC | 1 cut(s) | 223 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 156 |
| DpnII | GATC | 2 cut(s) | 40, 154 |
| EaeI | YGGCCR | 2 cut(s) | 75, 255 |
| EciI | GGCGGA | 1 cut(s) | 200 |
| Eco47I | GGWCC | 1 cut(s) | 187 |
| Eco57I | CTGAAG | 1 cut(s) | 305 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 194 |
| EcoRII | CCWGG | 1 cut(s) | 127 |
| FaeI | CATG | 1 cut(s) | 262 |
| FaiI | YATR | 4 cut(s) | 167, 169, 171, 260 |
| FatI | CATG | 1 cut(s) | 258 |
| FauI | CCCGC | 1 cut(s) | 79 |
| Fnu4HI | GCNGC | 3 cut(s) | 75, 133, 162 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 3 cut(s) | 75, 133, 162 |
| FspBI | CTAG | 1 cut(s) | 342 |
| GluI | GCNGC | 3 cut(s) | 75, 133, 162 |
| GsaI | CCCAGC | 1 cut(s) | 92 |
| HaeIII | GGCC | 4 cut(s) | 77, 122, 242, 257 |
| HapII | CCGG | 2 cut(s) | 146, 362 |
| Hin1II | CATG | 1 cut(s) | 262 |
| HinfI | GANTC | 1 cut(s) | 103 |
| HpaII | CCGG | 2 cut(s) | 146, 362 |
| Hpy188III | TCNNGA | 1 cut(s) | 152 |
| Hpy99I | CGWCG | 1 cut(s) | 281 |
| HpyAV | CCTTC | 2 cut(s) | 226, 283 |
| HpyCH4IV | ACGT | 1 cut(s) | 346 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 138, 299, 325 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 1 cut(s) | 346 |
| Hsp92II | CATG | 1 cut(s) | 262 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 40, 154 |
| LmnI | GCTCC | 1 cut(s) | 31 |
| LpnPI | CCDG | 9 cut(s) | 91, 102, 114, 137, 141, 159, 161, 167, 190 |
| Lsp1109I | GCAGC | 1 cut(s) | 144 |
| MaeI | CTAG | 1 cut(s) | 342 |
| MaeII | ACGT | 1 cut(s) | 346 |
| MalI | GATC | 2 cut(s) | 42, 156 |
| MboI | GATC | 2 cut(s) | 40, 154 |
| MboII | GAAGA | 1 cut(s) | 298 |
| MflI | RGATCY | 1 cut(s) | 154 |
| MlsI | TGGCCA | 1 cut(s) | 257 |
| MluCI | AATT | 1 cut(s) | 272 |
| MluNI | TGGCCA | 1 cut(s) | 257 |
| MlyI | GAGTC | 1 cut(s) | 97 |
| MmeI | TCCRAC | 1 cut(s) | 276 |
| MnlI | CCTC | 5 cut(s) | 65, 112, 200, 232, 292 |
| Mox20I | TGGCCA | 1 cut(s) | 257 |
| MscI | TGGCCA | 1 cut(s) | 257 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 263 |
| Msp20I | TGGCCA | 1 cut(s) | 257 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 92 |
| MspI | CCGG | 2 cut(s) | 146, 362 |
| MspR9I | CCNGG | 2 cut(s) | 129, 147 |
| MvaI | CCWGG | 1 cut(s) | 129 |
| MvnI | CGCG | 2 cut(s) | 74, 334 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 138, 299, 325 |
| NciI | CCSGG | 1 cut(s) | 147 |
| NdeII | GATC | 2 cut(s) | 40, 154 |
| NlaIII | CATG | 1 cut(s) | 262 |
| PkrI | GCNGC | 3 cut(s) | 76, 134, 163 |
| PleI | GAGTC | 1 cut(s) | 97 |
| PpsI | GAGTC | 1 cut(s) | 97 |
| Psp6I | CCWGG | 1 cut(s) | 127 |
| PspFI | CCCAGC | 1 cut(s) | 88 |
| PspGI | CCWGG | 1 cut(s) | 127 |
| PspPI | GGNCC | 3 cut(s) | 121, 187, 241 |
| PsuI | RGATCY | 1 cut(s) | 154 |
| PvuII | CAGCTG | 1 cut(s) | 92 |
| RsaI | GTAC | 1 cut(s) | 224 |
| RsaNI | GTAC | 1 cut(s) | 223 |
| RseI | CAYNNNNRTG | 1 cut(s) | 263 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 3 cut(s) | 75, 133, 162 |
| Sau3AI | GATC | 2 cut(s) | 40, 154 |
| Sau96I | GGNCC | 3 cut(s) | 121, 187, 241 |
| SchI | GAGTC | 1 cut(s) | 97 |
| ScrFI | CCNGG | 2 cut(s) | 129, 147 |
| SetI | ASST | 6 cut(s) | 57, 94, 177, 192, 237, 349 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 1 cut(s) | 187 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 263 |
| Sse9I | AATT | 1 cut(s) | 272 |
| SsiI | CCGC | 7 cut(s) | 38, 72, 74, 116, 162, 185, 365 |
| SspMI | CTAG | 1 cut(s) | 342 |
| StyD4I | CCNGG | 2 cut(s) | 127, 145 |
| TaiI | ACGT | 1 cut(s) | 349 |
| TaqI | TCGA | 2 cut(s) | 276, 351 |
| TasI | AATT | 1 cut(s) | 272 |
| TatI | WGTACW | 1 cut(s) | 222 |
| TauI | GCSGC | 2 cut(s) | 77, 164 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 186 |
| TseI | GCWGC | 1 cut(s) | 132 |
| TspRI | CASTG | 1 cut(s) | 186 |
| VpaK11BI | GGWCC | 1 cut(s) | 187 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 194 |
| XapI | RAATTY | 1 cut(s) | 272 |
| XspI | CTAG | 1 cut(s) | 342 |