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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 204 |
| AciI | CCGC | 3 cut(s) | 38, 153, 176 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AclWI | GGATC | 2 cut(s) | 48, 153 |
| AcuI | CTGAAG | 1 cut(s) | 296 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 241 |
| AjnI | CCWGG | 1 cut(s) | 141 |
| AlwI | GGATC | 2 cut(s) | 48, 153 |
| AoxI | GGCC | 2 cut(s) | 111, 231 |
| AspLEI | GCGC | 3 cut(s) | 204, 286, 310 |
| AspS9I | GGNCC | 3 cut(s) | 112, 178, 232 |
| AsuC2I | CCSGG | 1 cut(s) | 120 |
| AvaII | GGWCC | 1 cut(s) | 178 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 143 |
| BcnI | CCSGG | 1 cut(s) | 120 |
| BfoI | RGCGCY | 1 cut(s) | 287 |
| BisI | GCNGC | 1 cut(s) | 153 |
| BlsI | GCNGC | 1 cut(s) | 154 |
| Bme1390I | CCNGG | 2 cut(s) | 120, 143 |
| Bme18I | GGWCC | 1 cut(s) | 178 |
| BmgT120I | GGNCC | 3 cut(s) | 112, 178, 232 |
| BmrFI | CCNGG | 2 cut(s) | 120, 143 |
| BpmI | CTGGAG | 1 cut(s) | 308 |
| BpuMI | CCSGG | 1 cut(s) | 120 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 241 |
| BseBI | CCWGG | 1 cut(s) | 143 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 241 |
| BseYI | CCCAGC | 2 cut(s) | 88, 304 |
| Bsh1236I | CGCG | 1 cut(s) | 204 |
| Bsh1285I | CGRYCG | 1 cut(s) | 119 |
| BshFI | GGCC | 2 cut(s) | 113, 233 |
| BsiEI | CGRYCG | 1 cut(s) | 119 |
| BsiSI | CCGG | 1 cut(s) | 119 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 241 |
| BsnI | GGCC | 2 cut(s) | 113, 233 |
| Bsp143I | GATC | 2 cut(s) | 40, 145 |
| BspACI | CCGC | 3 cut(s) | 38, 153, 176 |
| BspANI | GGCC | 2 cut(s) | 113, 233 |
| BspFNI | CGCG | 1 cut(s) | 204 |
| BspPI | GGATC | 2 cut(s) | 48, 153 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BssMI | GATC | 2 cut(s) | 40, 145 |
| Bst2UI | CCWGG | 1 cut(s) | 143 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 317 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 1 cut(s) | 204 |
| BstH2I | RGCGCY | 1 cut(s) | 287 |
| BstHHI | GCGC | 3 cut(s) | 204, 286, 310 |
| BstKTI | GATC | 2 cut(s) | 43, 148 |
| BstMBI | GATC | 2 cut(s) | 40, 145 |
| BstMCI | CGRYCG | 1 cut(s) | 119 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 129, 138, 290, 305, 316 |
| BstNI | CCWGG | 1 cut(s) | 143 |
| BstSCI | CCNGG | 2 cut(s) | 118, 141 |
| BstUI | CGCG | 1 cut(s) | 204 |
| BstX2I | RGATCY | 1 cut(s) | 145 |
| BstYI | RGATCY | 1 cut(s) | 145 |
| BsuRI | GGCC | 2 cut(s) | 113, 233 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 1 cut(s) | 251 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 317 |
| CfoI | GCGC | 3 cut(s) | 204, 286, 310 |
| Cfr13I | GGNCC | 3 cut(s) | 112, 178, 232 |
| CviJI | RGCY | 4 cut(s) | 113, 141, 233, 248 |
| CviKI_1 | RGCY | 4 cut(s) | 113, 141, 233, 248 |
| DpnI | GATC | 2 cut(s) | 42, 147 |
| DpnII | GATC | 2 cut(s) | 40, 145 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 191 |
| Eco47I | GGWCC | 1 cut(s) | 178 |
| Eco57I | CTGAAG | 1 cut(s) | 296 |
| EcoRII | CCWGG | 1 cut(s) | 141 |
| FaiI | YATR | 3 cut(s) | 158, 162, 186 |
| Fnu4HI | GCNGC | 1 cut(s) | 153 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 1 cut(s) | 153 |
| GlaI | GCGC | 3 cut(s) | 203, 285, 309 |
| GluI | GCNGC | 1 cut(s) | 153 |
| GsaI | CCCAGC | 2 cut(s) | 92, 308 |
| GsuI | CTGGAG | 1 cut(s) | 308 |
| HaeII | RGCGCY | 1 cut(s) | 287 |
| HaeIII | GGCC | 2 cut(s) | 113, 233 |
| HapII | CCGG | 1 cut(s) | 119 |
| HhaI | GCGC | 3 cut(s) | 204, 286, 310 |
| Hin6I | GCGC | 3 cut(s) | 202, 284, 308 |
| HinP1I | GCGC | 3 cut(s) | 202, 284, 308 |
| HincII | GTYRAC | 4 cut(s) | 52, 85, 103, 168 |
| HindII | GTYRAC | 4 cut(s) | 52, 85, 103, 168 |
| HpaI | GTTAAC | 2 cut(s) | 85, 103 |
| HpaII | CCGG | 1 cut(s) | 119 |
| Hpy166II | GTNNAC | 4 cut(s) | 52, 85, 103, 168 |
| Hpy188III | TCNNGA | 1 cut(s) | 261 |
| Hpy8I | GTNNAC | 4 cut(s) | 52, 85, 103, 168 |
| HpyAV | CCTTC | 1 cut(s) | 274 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 129, 138, 290, 305, 316 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| HspAI | GCGC | 3 cut(s) | 202, 284, 308 |
| KspAI | GTTAAC | 2 cut(s) | 85, 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 145 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 199 |
| LpnPI | CCDG | 9 cut(s) | 102, 120, 128, 132, 155, 181, 227, 272, 318 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MaeIII | GTNAC | 1 cut(s) | 73 |
| MalI | GATC | 2 cut(s) | 42, 147 |
| MboI | GATC | 2 cut(s) | 40, 145 |
| MboII | GAAGA | 2 cut(s) | 41, 289 |
| MflI | RGATCY | 1 cut(s) | 145 |
| MnlI | CCTC | 5 cut(s) | 10, 103, 191, 223, 283 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MspI | CCGG | 1 cut(s) | 119 |
| MspR9I | CCNGG | 2 cut(s) | 120, 143 |
| MvaI | CCWGG | 1 cut(s) | 143 |
| MvnI | CGCG | 1 cut(s) | 204 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 129, 138, 290, 305, 316 |
| NciI | CCSGG | 1 cut(s) | 120 |
| NdeII | GATC | 2 cut(s) | 40, 145 |
| NmuCI | GTSAC | 1 cut(s) | 73 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 1 cut(s) | 154 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp6I | CCWGG | 1 cut(s) | 141 |
| PspFI | CCCAGC | 2 cut(s) | 88, 304 |
| PspGI | CCWGG | 1 cut(s) | 141 |
| PspPI | GGNCC | 3 cut(s) | 112, 178, 232 |
| PsuI | RGATCY | 1 cut(s) | 145 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 1 cut(s) | 153 |
| Sau3AI | GATC | 2 cut(s) | 40, 145 |
| Sau96I | GGNCC | 3 cut(s) | 112, 178, 232 |
| ScrFI | CCNGG | 2 cut(s) | 120, 143 |
| SetI | ASST | 5 cut(s) | 84, 102, 183, 228, 246 |
| SinI | GGWCC | 1 cut(s) | 178 |
| SsiI | CCGC | 3 cut(s) | 38, 153, 176 |
| StyD4I | CCNGG | 2 cut(s) | 118, 141 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 1 cut(s) | 228 |
| TauI | GCSGC | 1 cut(s) | 155 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TscAI | CASTG | 1 cut(s) | 258 |
| TseFI | GTSAC | 1 cut(s) | 73 |
| Tsp45I | GTSAC | 1 cut(s) | 73 |
| TspGWI | ACGGA | 1 cut(s) | 37 |
| TspRI | CASTG | 1 cut(s) | 258 |
| VpaK11BI | GGWCC | 1 cut(s) | 178 |