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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 136 |
| AccB1I | GGYRCC | 1 cut(s) | 136 |
| AccBSI | CCGCTC | 1 cut(s) | 416 |
| AccI | GTMKAC | 1 cut(s) | 88 |
| AciI | CCGC | 8 cut(s) | 96, 209, 281, 296, 414, 445, 462, 508 |
| AdeI | CACNNNGTG | 1 cut(s) | 74 |
| AfaI | GTAC | 1 cut(s) | 138 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 98, 280, 281 |
| AgsI | TTSAA | 1 cut(s) | 145 |
| AjnI | CCWGG | 2 cut(s) | 304, 431 |
| AleI | CACNNNNGTG | 1 cut(s) | 220 |
| AluBI | AGCT | 1 cut(s) | 157 |
| AluI | AGCT | 1 cut(s) | 157 |
| Alw21I | GWGCWC | 1 cut(s) | 227 |
| Alw44I | GTGCAC | 1 cut(s) | 223 |
| AoxI | GGCC | 5 cut(s) | 271, 379, 429, 486, 509 |
| ApaLI | GTGCAC | 1 cut(s) | 223 |
| Asp718I | GGTACC | 1 cut(s) | 136 |
| AspLEI | GCGC | 2 cut(s) | 31, 401 |
| AspS9I | GGNCC | 3 cut(s) | 272, 379, 435 |
| AsuHPI | GGTGA | 1 cut(s) | 434 |
| AvaII | GGWCC | 1 cut(s) | 435 |
| BaeGI | GKGCMC | 1 cut(s) | 227 |
| BanI | GGYRCC | 1 cut(s) | 136 |
| BbsI | GAAGAC | 1 cut(s) | 164 |
| Bbv12I | GWGCWC | 1 cut(s) | 227 |
| BbvCI | CCTCAGC | 1 cut(s) | 395 |
| BccI | CCATC | 1 cut(s) | 353 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 372, 406 |
| BciT130I | CCWGG | 2 cut(s) | 306, 433 |
| BfaI | CTAG | 4 cut(s) | 32, 78, 113, 243 |
| BfoI | RGCGCY | 2 cut(s) | 32, 402 |
| BisI | GCNGC | 1 cut(s) | 509 |
| BlsI | GCNGC | 1 cut(s) | 510 |
| Bme1390I | CCNGG | 2 cut(s) | 306, 433 |
| Bme18I | GGWCC | 1 cut(s) | 435 |
| BmgT120I | GGNCC | 3 cut(s) | 272, 379, 435 |
| BmiI | GGNNCC | 3 cut(s) | 138, 274, 300 |
| BmrFI | CCNGG | 2 cut(s) | 306, 433 |
| BpiI | GAAGAC | 1 cut(s) | 164 |
| BpmI | CTGGAG | 1 cut(s) | 288 |
| Bpu10I | CCTNAGC | 2 cut(s) | 153, 395 |
| BsaJI | CCNNGG | 1 cut(s) | 382 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 98, 280, 281 |
| Bse118I | RCCGGY | 1 cut(s) | 269 |
| Bse3DI | GCAATG | 1 cut(s) | 241 |
| BseBI | CCWGG | 2 cut(s) | 306, 433 |
| BseDI | CCNNGG | 1 cut(s) | 382 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 98, 280, 281 |
| BseMI | GCAATG | 1 cut(s) | 241 |
| BseMII | CTCAG | 2 cut(s) | 144, 409 |
| BseSI | GKGCMC | 1 cut(s) | 227 |
| BseYI | CCCAGC | 1 cut(s) | 92 |
| Bsh1285I | CGRYCG | 1 cut(s) | 270 |
| BshFI | GGCC | 5 cut(s) | 273, 381, 431, 488, 511 |
| BshNI | GGYRCC | 1 cut(s) | 136 |
| BsiEI | CGRYCG | 1 cut(s) | 270 |
| BsiHKAI | GWGCWC | 1 cut(s) | 227 |
| BsiSI | CCGG | 1 cut(s) | 270 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 98, 280, 281 |
| BsmI | GAATGC | 1 cut(s) | 355 |
| BsnI | GGCC | 5 cut(s) | 273, 381, 431, 488, 511 |
| Bsp1286I | GDGCHC | 1 cut(s) | 227 |
| BspACI | CCGC | 8 cut(s) | 96, 209, 281, 296, 414, 445, 462, 508 |
| BspANI | GGCC | 5 cut(s) | 273, 381, 431, 488, 511 |
| BspCNI | CTCAG | 2 cut(s) | 145, 408 |
| BspLI | GGNNCC | 3 cut(s) | 138, 274, 300 |
| BspT107I | GGYRCC | 1 cut(s) | 136 |
| BsrBI | CCGCTC | 1 cut(s) | 416 |
| BsrDI | GCAATG | 1 cut(s) | 241 |
| BsrFI | RCCGGY | 1 cut(s) | 269 |
| BssAI | RCCGGY | 1 cut(s) | 269 |
| BssECI | CCNNGG | 1 cut(s) | 382 |
| Bst2UI | CCWGG | 2 cut(s) | 306, 433 |
| Bst4CI | ACNGT | 2 cut(s) | 141, 222 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 231 |
| BstC8I | GCNNGC | 1 cut(s) | 460 |
| BstDEI | CTNAG | 2 cut(s) | 153, 395 |
| BstH2I | RGCGCY | 2 cut(s) | 32, 402 |
| BstHHI | GCGC | 2 cut(s) | 31, 401 |
| BstMCI | CGRYCG | 1 cut(s) | 270 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 35, 50, 231 |
| BstNI | CCWGG | 2 cut(s) | 306, 433 |
| BstNSI | RCATGY | 1 cut(s) | 480 |
| BstSCI | CCNGG | 2 cut(s) | 304, 431 |
| BstSLI | GKGCMC | 1 cut(s) | 227 |
| BstV2I | GAAGAC | 1 cut(s) | 164 |
| BsuRI | GGCC | 5 cut(s) | 273, 381, 431, 488, 511 |
| Cac8I | GCNNGC | 1 cut(s) | 460 |
| CfoI | GCGC | 2 cut(s) | 31, 401 |
| Cfr10I | RCCGGY | 1 cut(s) | 269 |
| Cfr13I | GGNCC | 3 cut(s) | 272, 379, 435 |
| Csp6I | GTAC | 1 cut(s) | 137 |
| CviAII | CATG | 2 cut(s) | 332, 477 |
| CviJI | RGCY | 7 cut(s) | 157, 273, 330, 381, 431, 488, 511 |
| CviKI_1 | RGCY | 7 cut(s) | 157, 273, 330, 381, 431, 488, 511 |
| CviQI | GTAC | 1 cut(s) | 137 |
| DdeI | CTNAG | 2 cut(s) | 153, 395 |
| DraIII | CACNNNGTG | 1 cut(s) | 74 |
| EciI | GGCGGA | 1 cut(s) | 224 |
| Eco47I | GGWCC | 1 cut(s) | 435 |
| EcoRII | CCWGG | 2 cut(s) | 304, 431 |
| FaeI | CATG | 2 cut(s) | 335, 480 |
| FaiI | YATR | 6 cut(s) | 84, 125, 293, 333, 450, 478 |
| FatI | CATG | 2 cut(s) | 331, 476 |
| FauI | CCCGC | 1 cut(s) | 274 |
| FblI | GTMKAC | 1 cut(s) | 88 |
| Fnu4HI | GCNGC | 1 cut(s) | 509 |
| Fsp4HI | GCNGC | 1 cut(s) | 509 |
| FspBI | CTAG | 4 cut(s) | 32, 78, 113, 243 |
| GlaI | GCGC | 2 cut(s) | 30, 400 |
| GluI | GCNGC | 1 cut(s) | 509 |
| GsaI | CCCAGC | 1 cut(s) | 96 |
| GsuI | CTGGAG | 1 cut(s) | 288 |
| HaeII | RGCGCY | 2 cut(s) | 32, 402 |
| HaeIII | GGCC | 5 cut(s) | 273, 381, 431, 488, 511 |
| HapII | CCGG | 1 cut(s) | 270 |
| HhaI | GCGC | 2 cut(s) | 31, 401 |
| Hin1II | CATG | 2 cut(s) | 335, 480 |
| Hin6I | GCGC | 2 cut(s) | 29, 399 |
| HinP1I | GCGC | 2 cut(s) | 29, 399 |
| HincII | GTYRAC | 1 cut(s) | 89 |
| HindII | GTYRAC | 1 cut(s) | 89 |
| HinfI | GANTC | 3 cut(s) | 212, 252, 374 |
| HpaII | CCGG | 1 cut(s) | 270 |
| HphI | GGTGA | 1 cut(s) | 434 |
| Hpy166II | GTNNAC | 3 cut(s) | 89, 225, 442 |
| Hpy188I | TCNGA | 1 cut(s) | 468 |
| Hpy188III | TCNNGA | 1 cut(s) | 6 |
| Hpy8I | GTNNAC | 3 cut(s) | 89, 225, 442 |
| HpyAV | CCTTC | 2 cut(s) | 19, 96 |
| HpyCH4III | ACNGT | 2 cut(s) | 141, 222 |
| HpyCH4V | TGCA | 6 cut(s) | 225, 234, 239, 353, 458, 480 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 35, 50, 231 |
| HpyF3I | CTNAG | 2 cut(s) | 153, 395 |
| Hsp92II | CATG | 2 cut(s) | 335, 480 |
| HspAI | GCGC | 2 cut(s) | 29, 399 |
| KpnI | GGTACC | 1 cut(s) | 140 |
| MaeI | CTAG | 4 cut(s) | 32, 78, 113, 243 |
| MbiI | CCGCTC | 1 cut(s) | 416 |
| MboII | GAAGA | 1 cut(s) | 164 |
| MhlI | GDGCHC | 1 cut(s) | 227 |
| MnlI | CCTC | 7 cut(s) | 189, 199, 250, 312, 326, 377, 404 |
| MslI | CAYNNNNRTG | 2 cut(s) | 220, 330 |
| MspA1I | CMGCKG | 2 cut(s) | 96, 508 |
| MspI | CCGG | 1 cut(s) | 270 |
| MspR9I | CCNGG | 2 cut(s) | 306, 433 |
| Mva1269I | GAATGC | 1 cut(s) | 355 |
| MvaI | CCWGG | 2 cut(s) | 306, 433 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 35, 50, 231 |
| NlaIII | CATG | 2 cut(s) | 335, 480 |
| NlaIV | GGNNCC | 3 cut(s) | 138, 274, 300 |
| NmeAIII | GCCGAG | 1 cut(s) | 407 |
| NspI | RCATGY | 1 cut(s) | 480 |
| OliI | CACNNNNGTG | 1 cut(s) | 220 |
| PctI | GAATGC | 1 cut(s) | 355 |
| PfeI | GAWTC | 3 cut(s) | 212, 252, 374 |
| PkrI | GCNGC | 1 cut(s) | 510 |
| Psp6I | CCWGG | 2 cut(s) | 304, 431 |
| PspFI | CCCAGC | 1 cut(s) | 92 |
| PspGI | CCWGG | 2 cut(s) | 304, 431 |
| PspN4I | GGNNCC | 3 cut(s) | 138, 274, 300 |
| PspPI | GGNCC | 3 cut(s) | 272, 379, 435 |
| RsaI | GTAC | 1 cut(s) | 138 |
| RsaNI | GTAC | 1 cut(s) | 137 |
| RseI | CAYNNNNRTG | 2 cut(s) | 220, 330 |
| SalI | GTCGAC | 1 cut(s) | 87 |
| SatI | GCNGC | 1 cut(s) | 509 |
| Sau96I | GGNCC | 3 cut(s) | 272, 379, 435 |
| ScrFI | CCNGG | 2 cut(s) | 306, 433 |
| SduI | GDGCHC | 1 cut(s) | 227 |
| SetI | ASST | 9 cut(s) | 107, 138, 154, 159, 181, 304, 310, 388, 437 |
| SinI | GGWCC | 1 cut(s) | 435 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 220, 330 |
| SsiI | CCGC | 8 cut(s) | 96, 209, 281, 296, 414, 445, 462, 508 |
| SspMI | CTAG | 4 cut(s) | 32, 78, 113, 243 |
| StyD4I | CCNGG | 2 cut(s) | 304, 431 |
| TaaI | ACNGT | 2 cut(s) | 141, 222 |
| TaqI | TCGA | 2 cut(s) | 88, 255 |
| TauI | GCSGC | 1 cut(s) | 511 |
| TfiI | GAWTC | 3 cut(s) | 212, 252, 374 |
| TspDTI | ATGAA | 1 cut(s) | 30 |
| TspGWI | ACGGA | 1 cut(s) | 427 |
| VneI | GTGCAC | 1 cut(s) | 223 |
| VpaK11BI | GGWCC | 1 cut(s) | 435 |
| XceI | RCATGY | 1 cut(s) | 480 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 338 |
| XmiI | GTMKAC | 1 cut(s) | 88 |
| XspI | CTAG | 4 cut(s) | 32, 78, 113, 243 |