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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 90, 219 |
| AciI | CCGC | 8 cut(s) | 9, 15, 48, 74, 88, 107, 145, 191 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 116, 151 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 1 cut(s) | 246 |
| ApeKI | GCWGC | 2 cut(s) | 138, 141 |
| AspLEI | GCGC | 1 cut(s) | 92 |
| AspS9I | GGNCC | 1 cut(s) | 247 |
| AsuC2I | CCSGG | 2 cut(s) | 117, 153 |
| BbvCI | CCTCAGC | 1 cut(s) | 243 |
| BbvI | GCAGC | 2 cut(s) | 150, 153 |
| BccI | CCATC | 1 cut(s) | 59 |
| BcnI | CCSGG | 2 cut(s) | 117, 153 |
| BfaI | CTAG | 3 cut(s) | 6, 71, 188 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 3 cut(s) | 139, 142, 145 |
| BlsI | GCNGC | 3 cut(s) | 140, 143, 146 |
| Bme1390I | CCNGG | 2 cut(s) | 117, 153 |
| BmgT120I | GGNCC | 1 cut(s) | 247 |
| BmrFI | CCNGG | 2 cut(s) | 117, 153 |
| Bpu10I | CCTNAGC | 1 cut(s) | 243 |
| BpuMI | CCSGG | 2 cut(s) | 117, 153 |
| BsaJI | CCNNGG | 1 cut(s) | 115 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 116, 151 |
| BseDI | CCNNGG | 1 cut(s) | 115 |
| BseGI | GGATG | 1 cut(s) | 51 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 116, 151 |
| BseMII | CTCAG | 1 cut(s) | 234 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 2 cut(s) | 150, 153 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 219 |
| BshFI | GGCC | 1 cut(s) | 248 |
| BsiSI | CCGG | 2 cut(s) | 117, 152 |
| BslFI | GGGAC | 1 cut(s) | 168 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 116, 151 |
| BsmFI | GGGAC | 1 cut(s) | 168 |
| BsnI | GGCC | 1 cut(s) | 248 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspACI | CCGC | 8 cut(s) | 9, 15, 48, 74, 88, 107, 145, 191 |
| BspANI | GGCC | 1 cut(s) | 248 |
| BspCNI | CTCAG | 1 cut(s) | 235 |
| BspFNI | CGCG | 2 cut(s) | 90, 219 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BssECI | CCNNGG | 1 cut(s) | 115 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 149 |
| BstDEI | CTNAG | 1 cut(s) | 243 |
| BstF5I | GGATG | 1 cut(s) | 51 |
| BstFNI | CGCG | 2 cut(s) | 90, 219 |
| BstHHI | GCGC | 1 cut(s) | 92 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 144 |
| BstSCI | CCNGG | 2 cut(s) | 115, 151 |
| BstUI | CGCG | 2 cut(s) | 90, 219 |
| BstV1I | GCAGC | 2 cut(s) | 150, 153 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 1 cut(s) | 248 |
| BtsCI | GGATG | 1 cut(s) | 51 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 149 |
| CfoI | GCGC | 1 cut(s) | 92 |
| Cfr13I | GGNCC | 1 cut(s) | 247 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CviJI | RGCY | 4 cut(s) | 26, 144, 242, 248 |
| CviKI_1 | RGCY | 4 cut(s) | 26, 144, 242, 248 |
| CviQI | GTAC | 1 cut(s) | 175 |
| DdeI | CTNAG | 1 cut(s) | 243 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 37 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| FaiI | YATR | 2 cut(s) | 173, 201 |
| FaqI | GGGAC | 1 cut(s) | 168 |
| FauI | CCCGC | 2 cut(s) | 8, 114 |
| Fnu4HI | GCNGC | 3 cut(s) | 139, 142, 145 |
| FokI | GGATG | 1 cut(s) | 38 |
| Fsp4HI | GCNGC | 3 cut(s) | 139, 142, 145 |
| FspBI | CTAG | 3 cut(s) | 6, 71, 188 |
| GlaI | GCGC | 1 cut(s) | 91 |
| GluI | GCNGC | 3 cut(s) | 139, 142, 145 |
| HaeIII | GGCC | 1 cut(s) | 248 |
| HapII | CCGG | 2 cut(s) | 117, 152 |
| HhaI | GCGC | 1 cut(s) | 92 |
| Hin6I | GCGC | 1 cut(s) | 90 |
| HinP1I | GCGC | 1 cut(s) | 90 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 2 cut(s) | 117, 152 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 144 |
| HpyF3I | CTNAG | 1 cut(s) | 243 |
| HspAI | GCGC | 1 cut(s) | 90 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| LpnPI | CCDG | 4 cut(s) | 20, 130, 161, 165 |
| Lsp1109I | GCAGC | 2 cut(s) | 150, 153 |
| MaeI | CTAG | 3 cut(s) | 6, 71, 188 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 5 cut(s) | 73, 118, 214, 217, 238 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 170 |
| MspI | CCGG | 2 cut(s) | 117, 152 |
| MspR9I | CCNGG | 2 cut(s) | 117, 153 |
| MvnI | CGCG | 2 cut(s) | 90, 219 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 144 |
| NciI | CCSGG | 2 cut(s) | 117, 153 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 3 cut(s) | 140, 143, 146 |
| PspPI | GGNCC | 1 cut(s) | 247 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| RseI | CAYNNNNRTG | 1 cut(s) | 170 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 3 cut(s) | 139, 142, 145 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 1 cut(s) | 247 |
| ScrFI | CCNGG | 2 cut(s) | 117, 153 |
| SetI | ASST | 1 cut(s) | 72 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 170 |
| SsiI | CCGC | 8 cut(s) | 9, 15, 48, 74, 88, 107, 145, 191 |
| SspMI | CTAG | 3 cut(s) | 6, 71, 188 |
| StyD4I | CCNGG | 2 cut(s) | 115, 151 |
| TauI | GCSGC | 1 cut(s) | 147 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TseI | GCWGC | 2 cut(s) | 138, 141 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| XspI | CTAG | 3 cut(s) | 6, 71, 188 |