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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 466 |
| Acc65I | GGTACC | 2 cut(s) | 451, 478 |
| AccB1I | GGYRCC | 2 cut(s) | 451, 478 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 633 |
| AccI | GTMKAC | 2 cut(s) | 150, 430 |
| AccII | CGCG | 2 cut(s) | 162, 349 |
| AclWI | GGATC | 1 cut(s) | 273 |
| AcoI | YGGCCR | 1 cut(s) | 180 |
| AcuI | CTGAAG | 2 cut(s) | 383, 618 |
| AfaI | GTAC | 3 cut(s) | 248, 453, 480 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 223, 440, 633 |
| AluBI | AGCT | 3 cut(s) | 253, 380, 584 |
| AluI | AGCT | 3 cut(s) | 253, 380, 584 |
| AlwI | GGATC | 1 cut(s) | 273 |
| AoxI | GGCC | 5 cut(s) | 98, 180, 226, 318, 458 |
| ApeKI | GCWGC | 3 cut(s) | 65, 210, 213 |
| Asp718I | GGTACC | 2 cut(s) | 451, 478 |
| AspLEI | GCGC | 2 cut(s) | 164, 397 |
| AspS9I | GGNCC | 5 cut(s) | 98, 226, 319, 487, 512 |
| AsuC2I | CCSGG | 3 cut(s) | 179, 189, 435 |
| AsuNHI | GCTAGC | 1 cut(s) | 253 |
| AvaII | GGWCC | 2 cut(s) | 487, 512 |
| BanI | GGYRCC | 2 cut(s) | 451, 478 |
| BbvCI | CCTCAGC | 1 cut(s) | 315 |
| BbvI | GCAGC | 3 cut(s) | 52, 222, 225 |
| BccI | CCATC | 6 cut(s) | 25, 52, 98, 131, 502, 530 |
| BceAI | ACGGC | 2 cut(s) | 42, 87 |
| BcnI | CCSGG | 3 cut(s) | 179, 189, 435 |
| BfaI | CTAG | 4 cut(s) | 5, 110, 254, 585 |
| BfuAI | ACCTGC | 1 cut(s) | 466 |
| BglII | AGATCT | 1 cut(s) | 232 |
| BisI | GCNGC | 4 cut(s) | 66, 211, 214, 563 |
| BlsI | GCNGC | 4 cut(s) | 67, 212, 215, 564 |
| Bme1390I | CCNGG | 3 cut(s) | 179, 189, 435 |
| Bme18I | GGWCC | 2 cut(s) | 487, 512 |
| BmgT120I | GGNCC | 5 cut(s) | 98, 226, 319, 487, 512 |
| BmiI | GGNNCC | 4 cut(s) | 453, 480, 489, 513 |
| BmrFI | CCNGG | 3 cut(s) | 179, 189, 435 |
| BmsI | GCATC | 3 cut(s) | 462, 606, 632 |
| BmtI | GCTAGC | 1 cut(s) | 257 |
| Bpu10I | CCTNAGC | 1 cut(s) | 315 |
| BpuMI | CCSGG | 3 cut(s) | 179, 189, 435 |
| BsaAI | YACGTR | 1 cut(s) | 413 |
| BsaJI | CCNNGG | 3 cut(s) | 177, 187, 455 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 646 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 223, 440, 633 |
| Bse118I | RCCGGY | 1 cut(s) | 610 |
| Bse1I | ACTGG | 3 cut(s) | 445, 461, 656 |
| BseDI | CCNNGG | 3 cut(s) | 177, 187, 455 |
| BseGI | GGATG | 6 cut(s) | 21, 109, 123, 513, 522, 647 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 223, 440, 633 |
| BseMII | CTCAG | 2 cut(s) | 306, 486 |
| BseNI | ACTGG | 3 cut(s) | 445, 461, 656 |
| BseRI | GAGGAG | 1 cut(s) | 308 |
| BseXI | GCAGC | 3 cut(s) | 52, 222, 225 |
| BseYI | CCCAGC | 1 cut(s) | 142 |
| Bsh1236I | CGCG | 2 cut(s) | 162, 349 |
| BshFI | GGCC | 5 cut(s) | 100, 182, 228, 320, 460 |
| BshNI | GGYRCC | 2 cut(s) | 451, 478 |
| BsiSI | CCGG | 5 cut(s) | 101, 179, 189, 435, 611 |
| BslFI | GGGAC | 1 cut(s) | 525 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 223, 440, 633 |
| BsmFI | GGGAC | 1 cut(s) | 525 |
| BsnI | GGCC | 5 cut(s) | 100, 182, 228, 320, 460 |
| Bsp143I | GATC | 2 cut(s) | 232, 265 |
| BspANI | GGCC | 5 cut(s) | 100, 182, 228, 320, 460 |
| BspCNI | CTCAG | 2 cut(s) | 307, 487 |
| BspFNI | CGCG | 2 cut(s) | 162, 349 |
| BspLI | GGNNCC | 4 cut(s) | 453, 480, 489, 513 |
| BspMI | ACCTGC | 1 cut(s) | 466 |
| BspOI | GCTAGC | 1 cut(s) | 257 |
| BspPI | GGATC | 1 cut(s) | 273 |
| BspT107I | GGYRCC | 2 cut(s) | 451, 478 |
| BsrFI | RCCGGY | 1 cut(s) | 610 |
| BsrI | ACTGG | 3 cut(s) | 445, 461, 656 |
| BssAI | RCCGGY | 1 cut(s) | 610 |
| BssECI | CCNNGG | 3 cut(s) | 177, 187, 455 |
| BssMI | GATC | 2 cut(s) | 232, 265 |
| Bst4CI | ACNGT | 2 cut(s) | 330, 408 |
| Bst6I | CTCTTC | 1 cut(s) | 359 |
| BstBAI | YACGTR | 1 cut(s) | 413 |
| BstC8I | GCNNGC | 2 cut(s) | 255, 612 |
| BstDEI | CTNAG | 2 cut(s) | 315, 495 |
| BstF5I | GGATG | 6 cut(s) | 21, 109, 123, 513, 522, 647 |
| BstFNI | CGCG | 2 cut(s) | 162, 349 |
| BstHHI | GCGC | 2 cut(s) | 164, 397 |
| BstKTI | GATC | 2 cut(s) | 235, 268 |
| BstMBI | GATC | 2 cut(s) | 232, 265 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 71, 346, 392, 481, 616 |
| BstSCI | CCNGG | 3 cut(s) | 177, 187, 433 |
| BstUI | CGCG | 2 cut(s) | 162, 349 |
| BstV1I | GCAGC | 3 cut(s) | 52, 222, 225 |
| BstX2I | RGATCY | 2 cut(s) | 232, 265 |
| BstYI | RGATCY | 2 cut(s) | 232, 265 |
| BsuRI | GGCC | 5 cut(s) | 100, 182, 228, 320, 460 |
| BtgZI | GCGATG | 2 cut(s) | 44, 629 |
| BtsCI | GGATG | 6 cut(s) | 21, 109, 123, 513, 522, 647 |
| BtsI | GCAGTG | 1 cut(s) | 557 |
| BtsIMutI | CAGTG | 3 cut(s) | 27, 438, 557 |
| BveI | ACCTGC | 1 cut(s) | 466 |
| Cac8I | GCNNGC | 2 cut(s) | 255, 612 |
| CfoI | GCGC | 2 cut(s) | 164, 397 |
| Cfr10I | RCCGGY | 1 cut(s) | 610 |
| Cfr13I | GGNCC | 5 cut(s) | 98, 226, 319, 487, 512 |
| CseI | GACGC | 1 cut(s) | 379 |
| Csp6I | GTAC | 3 cut(s) | 247, 452, 479 |
| CviQI | GTAC | 3 cut(s) | 247, 452, 479 |
| DdeI | CTNAG | 2 cut(s) | 315, 495 |
| DpnI | GATC | 2 cut(s) | 234, 267 |
| DpnII | GATC | 2 cut(s) | 232, 265 |
| EaeI | YGGCCR | 1 cut(s) | 180 |
| Eam1104I | CTCTTC | 1 cut(s) | 359 |
| EarI | CTCTTC | 1 cut(s) | 359 |
| EciI | GGCGGA | 3 cut(s) | 278, 566, 597 |
| Eco47I | GGWCC | 2 cut(s) | 487, 512 |
| Eco57I | CTGAAG | 2 cut(s) | 383, 618 |
| FaiI | YATR | 6 cut(s) | 44, 94, 96, 245, 273, 467 |
| FaqI | GGGAC | 1 cut(s) | 525 |
| FauI | CCCGC | 1 cut(s) | 228 |
| FauNDI | CATATG | 1 cut(s) | 94 |
| FblI | GTMKAC | 2 cut(s) | 150, 430 |
| Fnu4HI | GCNGC | 4 cut(s) | 66, 211, 214, 563 |
| FokI | GGATG | 6 cut(s) | 28, 110, 116, 509, 520, 654 |
| Fsp4HI | GCNGC | 4 cut(s) | 66, 211, 214, 563 |
| FspBI | CTAG | 4 cut(s) | 5, 110, 254, 585 |
| GlaI | GCGC | 2 cut(s) | 163, 396 |
| GluI | GCNGC | 4 cut(s) | 66, 211, 214, 563 |
| GsaI | CCCAGC | 1 cut(s) | 146 |
| HaeIII | GGCC | 5 cut(s) | 100, 182, 228, 320, 460 |
| HapII | CCGG | 5 cut(s) | 101, 179, 189, 435, 611 |
| HgaI | GACGC | 1 cut(s) | 379 |
| HhaI | GCGC | 2 cut(s) | 164, 397 |
| Hin6I | GCGC | 2 cut(s) | 162, 395 |
| HinP1I | GCGC | 2 cut(s) | 162, 395 |
| HincII | GTYRAC | 1 cut(s) | 431 |
| HindII | GTYRAC | 1 cut(s) | 431 |
| HinfI | GANTC | 1 cut(s) | 554 |
| HpaII | CCGG | 5 cut(s) | 101, 179, 189, 435, 611 |
| Hpy166II | GTNNAC | 2 cut(s) | 151, 431 |
| Hpy188I | TCNGA | 2 cut(s) | 139, 580 |
| Hpy8I | GTNNAC | 2 cut(s) | 151, 431 |
| HpyAV | CCTTC | 2 cut(s) | 304, 593 |
| HpyCH4III | ACNGT | 2 cut(s) | 330, 408 |
| HpyCH4IV | ACGT | 4 cut(s) | 153, 289, 412, 424 |
| HpyCH4V | TGCA | 4 cut(s) | 446, 475, 619, 645 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 71, 346, 392, 481, 616 |
| HpyF3I | CTNAG | 2 cut(s) | 315, 495 |
| HpySE526I | ACGT | 4 cut(s) | 153, 289, 412, 424 |
| HspAI | GCGC | 2 cut(s) | 162, 395 |
| KpnI | GGTACC | 2 cut(s) | 455, 482 |
| KroI | GCCGGC | 1 cut(s) | 610 |
| KroNI | GCCGGC | 1 cut(s) | 612 |
| Kzo9I | GATC | 2 cut(s) | 232, 265 |
| LmnI | GCTCC | 2 cut(s) | 78, 654 |
| Lsp1109I | GCAGC | 3 cut(s) | 52, 222, 225 |
| LweI | GCATC | 3 cut(s) | 462, 606, 632 |
| MaeI | CTAG | 4 cut(s) | 5, 110, 254, 585 |
| MaeII | ACGT | 4 cut(s) | 153, 289, 412, 424 |
| MaeIII | GTNAC | 2 cut(s) | 22, 408 |
| MalI | GATC | 2 cut(s) | 234, 267 |
| MboI | GATC | 2 cut(s) | 232, 265 |
| MboII | GAAGA | 2 cut(s) | 260, 376 |
| MflI | RGATCY | 2 cut(s) | 232, 265 |
| MluCI | AATT | 1 cut(s) | 116 |
| MmeI | TCCRAC | 1 cut(s) | 162 |
| MreI | CGCCGGCG | 1 cut(s) | 610 |
| MroNI | GCCGGC | 1 cut(s) | 610 |
| MseI | TTAA | 1 cut(s) | 156 |
| MslI | CAYNNNNRTG | 2 cut(s) | 242, 470 |
| MspA1I | CMGCKG | 2 cut(s) | 146, 484 |
| MspI | CCGG | 5 cut(s) | 101, 179, 189, 435, 611 |
| MspR9I | CCNGG | 3 cut(s) | 179, 189, 435 |
| MvnI | CGCG | 2 cut(s) | 162, 349 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 71, 346, 392, 481, 616 |
| NaeI | GCCGGC | 1 cut(s) | 612 |
| NciI | CCSGG | 3 cut(s) | 179, 189, 435 |
| NdeI | CATATG | 1 cut(s) | 94 |
| NdeII | GATC | 2 cut(s) | 232, 265 |
| NgoMIV | GCCGGC | 1 cut(s) | 610 |
| NheI | GCTAGC | 1 cut(s) | 253 |
| NlaIV | GGNNCC | 4 cut(s) | 453, 480, 489, 513 |
| NmuCI | GTSAC | 2 cut(s) | 22, 408 |
| PdiI | GCCGGC | 1 cut(s) | 612 |
| PfeI | GAWTC | 1 cut(s) | 554 |
| PflFI | GACNNNGTC | 1 cut(s) | 426 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 633 |
| PkrI | GCNGC | 4 cut(s) | 67, 212, 215, 564 |
| Ppu21I | YACGTR | 1 cut(s) | 413 |
| PspFI | CCCAGC | 1 cut(s) | 142 |
| PspN4I | GGNNCC | 4 cut(s) | 453, 480, 489, 513 |
| PspPI | GGNCC | 5 cut(s) | 98, 226, 319, 487, 512 |
| PsuI | RGATCY | 2 cut(s) | 232, 265 |
| PsyI | GACNNNGTC | 1 cut(s) | 426 |
| RsaI | GTAC | 3 cut(s) | 248, 453, 480 |
| RsaNI | GTAC | 3 cut(s) | 247, 452, 479 |
| RseI | CAYNNNNRTG | 2 cut(s) | 242, 470 |
| SalI | GTCGAC | 1 cut(s) | 429 |
| SaqAI | TTAA | 1 cut(s) | 156 |
| SatI | GCNGC | 4 cut(s) | 66, 211, 214, 563 |
| Sau3AI | GATC | 2 cut(s) | 232, 265 |
| Sau96I | GGNCC | 5 cut(s) | 98, 226, 319, 487, 512 |
| ScrFI | CCNGG | 3 cut(s) | 179, 189, 435 |
| SfaNI | GCATC | 3 cut(s) | 462, 606, 632 |
| SgrAI | CRCCGGYG | 1 cut(s) | 610 |
| SinI | GGWCC | 2 cut(s) | 487, 512 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 242, 470 |
| Sse9I | AATT | 1 cut(s) | 116 |
| SspMI | CTAG | 4 cut(s) | 5, 110, 254, 585 |
| StyD4I | CCNGG | 3 cut(s) | 177, 187, 433 |
| TaaI | ACNGT | 2 cut(s) | 330, 408 |
| TaiI | ACGT | 4 cut(s) | 156, 292, 415, 427 |
| TaqI | TCGA | 1 cut(s) | 430 |
| TasI | AATT | 1 cut(s) | 116 |
| TauI | GCSGC | 1 cut(s) | 565 |
| TfiI | GAWTC | 1 cut(s) | 554 |
| Tru1I | TTAA | 1 cut(s) | 156 |
| Tru9I | TTAA | 1 cut(s) | 156 |
| TscAI | CASTG | 3 cut(s) | 27, 445, 564 |
| TseFI | GTSAC | 2 cut(s) | 22, 408 |
| TseI | GCWGC | 3 cut(s) | 65, 210, 213 |
| Tsp45I | GTSAC | 2 cut(s) | 22, 408 |
| TspDTI | ATGAA | 2 cut(s) | 260, 372 |
| TspRI | CASTG | 3 cut(s) | 27, 445, 564 |
| Tth111I | GACNNNGTC | 1 cut(s) | 426 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 633 |
| VpaK11BI | GGWCC | 2 cut(s) | 487, 512 |
| XmiI | GTMKAC | 2 cut(s) | 150, 430 |
| XspI | CTAG | 4 cut(s) | 5, 110, 254, 585 |