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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 239 |
| AccBSI | CCGCTC | 1 cut(s) | 366 |
| AccII | CGCG | 2 cut(s) | 84, 359 |
| AciI | CCGC | 5 cut(s) | 66, 82, 323, 335, 366 |
| AclWI | GGATC | 5 cut(s) | 119, 266, 277, 322, 335 |
| AcoI | YGGCCR | 1 cut(s) | 67 |
| AcyI | GRCGYC | 2 cut(s) | 33, 201 |
| AfaI | GTAC | 1 cut(s) | 62 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 281 |
| AgsI | TTSAA | 2 cut(s) | 9, 236 |
| AluBI | AGCT | 1 cut(s) | 151 |
| AluI | AGCT | 1 cut(s) | 151 |
| Alw26I | GTCTC | 1 cut(s) | 178 |
| AlwI | GGATC | 5 cut(s) | 119, 266, 277, 322, 335 |
| AoxI | GGCC | 3 cut(s) | 67, 292, 349 |
| ApeKI | GCWGC | 2 cut(s) | 23, 320 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| AspLEI | GCGC | 2 cut(s) | 23, 28 |
| AsuC2I | CCSGG | 2 cut(s) | 129, 348 |
| AsuHPI | GGTGA | 4 cut(s) | 236, 264, 275, 349 |
| BaeGI | GKGCMC | 2 cut(s) | 138, 244 |
| BamHI | GGATCC | 1 cut(s) | 327 |
| BanI | GGYRCC | 1 cut(s) | 239 |
| BbvI | GCAGC | 2 cut(s) | 10, 307 |
| BccI | CCATC | 1 cut(s) | 302 |
| BceAI | ACGGC | 2 cut(s) | 79, 333 |
| BcnI | CCSGG | 2 cut(s) | 129, 348 |
| BcoDI | GTCTC | 1 cut(s) | 178 |
| BfaI | CTAG | 1 cut(s) | 332 |
| BisI | GCNGC | 3 cut(s) | 24, 67, 321 |
| BlsI | GCNGC | 3 cut(s) | 25, 68, 322 |
| Bme1390I | CCNGG | 2 cut(s) | 129, 348 |
| BmiI | GGNNCC | 2 cut(s) | 241, 329 |
| BmrFI | CCNGG | 2 cut(s) | 129, 348 |
| BmsI | GCATC | 1 cut(s) | 294 |
| BpuMI | CCSGG | 2 cut(s) | 129, 348 |
| BsaHI | GRCGYC | 2 cut(s) | 33, 201 |
| BsaJI | CCNNGG | 1 cut(s) | 70 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 281 |
| Bse3DI | GCAATG | 1 cut(s) | 153 |
| BseDI | CCNNGG | 1 cut(s) | 70 |
| BseGI | GGATG | 1 cut(s) | 81 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 281 |
| BseMI | GCAATG | 1 cut(s) | 153 |
| BseSI | GKGCMC | 2 cut(s) | 138, 244 |
| BseXI | GCAGC | 2 cut(s) | 10, 307 |
| Bsh1236I | CGCG | 2 cut(s) | 84, 359 |
| BshFI | GGCC | 3 cut(s) | 69, 294, 351 |
| BshNI | GGYRCC | 1 cut(s) | 239 |
| BsiSI | CCGG | 2 cut(s) | 129, 348 |
| BslFI | GGGAC | 1 cut(s) | 330 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 281 |
| BsmAI | GTCTC | 1 cut(s) | 178 |
| BsmFI | GGGAC | 1 cut(s) | 330 |
| BsnI | GGCC | 3 cut(s) | 69, 294, 351 |
| Bsp1286I | GDGCHC | 2 cut(s) | 138, 244 |
| Bsp143I | GATC | 4 cut(s) | 124, 258, 269, 327 |
| BspACI | CCGC | 5 cut(s) | 66, 82, 323, 335, 366 |
| BspANI | GGCC | 3 cut(s) | 69, 294, 351 |
| BspFNI | CGCG | 2 cut(s) | 84, 359 |
| BspLI | GGNNCC | 2 cut(s) | 241, 329 |
| BspPI | GGATC | 5 cut(s) | 119, 266, 277, 322, 335 |
| BspQI | GCTCTTC | 1 cut(s) | 356 |
| BspT107I | GGYRCC | 1 cut(s) | 239 |
| BsrBI | CCGCTC | 1 cut(s) | 366 |
| BsrDI | GCAATG | 1 cut(s) | 153 |
| BssECI | CCNNGG | 1 cut(s) | 70 |
| BssMI | GATC | 4 cut(s) | 124, 258, 269, 327 |
| BssNI | GRCGYC | 2 cut(s) | 33, 201 |
| Bst4CI | ACNGT | 1 cut(s) | 276 |
| Bst6I | CTCTTC | 1 cut(s) | 356 |
| BstACI | GRCGYC | 2 cut(s) | 33, 201 |
| BstDSI | CCRYGG | 1 cut(s) | 70 |
| BstF5I | GGATG | 1 cut(s) | 81 |
| BstFNI | CGCG | 2 cut(s) | 84, 359 |
| BstHHI | GCGC | 2 cut(s) | 23, 28 |
| BstKTI | GATC | 4 cut(s) | 127, 261, 272, 330 |
| BstMAI | GTCTC | 1 cut(s) | 178 |
| BstMBI | GATC | 4 cut(s) | 124, 258, 269, 327 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 32, 291 |
| BstNSI | RCATGY | 1 cut(s) | 306 |
| BstSCI | CCNGG | 2 cut(s) | 127, 346 |
| BstSLI | GKGCMC | 2 cut(s) | 138, 244 |
| BstUI | CGCG | 2 cut(s) | 84, 359 |
| BstV1I | GCAGC | 2 cut(s) | 10, 307 |
| BstX2I | RGATCY | 2 cut(s) | 124, 327 |
| BstYI | RGATCY | 2 cut(s) | 124, 327 |
| BsuRI | GGCC | 3 cut(s) | 69, 294, 351 |
| BtgI | CCRYGG | 1 cut(s) | 70 |
| BtsCI | GGATG | 1 cut(s) | 81 |
| CfoI | GCGC | 2 cut(s) | 23, 28 |
| CseI | GACGC | 2 cut(s) | 41, 190 |
| Csp6I | GTAC | 1 cut(s) | 61 |
| CviAII | CATG | 2 cut(s) | 108, 303 |
| CviJI | RGCY | 7 cut(s) | 47, 69, 151, 294, 312, 320, 351 |
| CviKI_1 | RGCY | 7 cut(s) | 47, 69, 151, 294, 312, 320, 351 |
| CviQI | GTAC | 1 cut(s) | 61 |
| DpnI | GATC | 4 cut(s) | 126, 260, 271, 329 |
| DpnII | GATC | 4 cut(s) | 124, 258, 269, 327 |
| EaeI | YGGCCR | 1 cut(s) | 67 |
| Eam1104I | CTCTTC | 1 cut(s) | 356 |
| EarI | CTCTTC | 1 cut(s) | 356 |
| FaeI | CATG | 2 cut(s) | 111, 306 |
| FaiI | YATR | 4 cut(s) | 109, 246, 248, 304 |
| FaqI | GGGAC | 1 cut(s) | 330 |
| FatI | CATG | 2 cut(s) | 107, 302 |
| FauI | CCCGC | 1 cut(s) | 316 |
| FauNDI | CATATG | 1 cut(s) | 246 |
| Fnu4HI | GCNGC | 3 cut(s) | 24, 67, 321 |
| FokI | GGATG | 1 cut(s) | 88 |
| Fsp4HI | GCNGC | 3 cut(s) | 24, 67, 321 |
| FspBI | CTAG | 1 cut(s) | 332 |
| GlaI | GCGC | 2 cut(s) | 22, 27 |
| GluI | GCNGC | 3 cut(s) | 24, 67, 321 |
| HaeIII | GGCC | 3 cut(s) | 69, 294, 351 |
| HapII | CCGG | 2 cut(s) | 129, 348 |
| HgaI | GACGC | 2 cut(s) | 41, 190 |
| HhaI | GCGC | 2 cut(s) | 23, 28 |
| Hin1I | GRCGYC | 2 cut(s) | 33, 201 |
| Hin1II | CATG | 2 cut(s) | 111, 306 |
| Hin6I | GCGC | 2 cut(s) | 21, 26 |
| HinP1I | GCGC | 2 cut(s) | 21, 26 |
| HinfI | GANTC | 1 cut(s) | 116 |
| HpaII | CCGG | 2 cut(s) | 129, 348 |
| HphI | GGTGA | 4 cut(s) | 236, 264, 275, 349 |
| HpyAV | CCTTC | 3 cut(s) | 15, 230, 248 |
| HpyCH4III | ACNGT | 1 cut(s) | 276 |
| HpyCH4V | TGCA | 2 cut(s) | 107, 302 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 32, 291 |
| Hsp92I | GRCGYC | 2 cut(s) | 33, 201 |
| Hsp92II | CATG | 2 cut(s) | 111, 306 |
| HspAI | GCGC | 2 cut(s) | 21, 26 |
| Kzo9I | GATC | 4 cut(s) | 124, 258, 269, 327 |
| LguI | GCTCTTC | 1 cut(s) | 356 |
| LpnPI | CCDG | 3 cut(s) | 142, 161, 361 |
| Lsp1109I | GCAGC | 2 cut(s) | 10, 307 |
| LweI | GCATC | 1 cut(s) | 294 |
| MaeI | CTAG | 1 cut(s) | 332 |
| MaeIII | GTNAC | 1 cut(s) | 224 |
| MalI | GATC | 4 cut(s) | 126, 260, 271, 329 |
| MbiI | CCGCTC | 1 cut(s) | 366 |
| MboI | GATC | 4 cut(s) | 124, 258, 269, 327 |
| MboII | GAAGA | 2 cut(s) | 154, 373 |
| MfeI | CAATTG | 1 cut(s) | 41 |
| MflI | RGATCY | 2 cut(s) | 124, 327 |
| MhlI | GDGCHC | 2 cut(s) | 138, 244 |
| MluCI | AATT | 1 cut(s) | 41 |
| MlyI | GAGTC | 1 cut(s) | 125 |
| MnlI | CCTC | 3 cut(s) | 169, 187, 259 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MseI | TTAA | 2 cut(s) | 354, 373 |
| MspI | CCGG | 2 cut(s) | 129, 348 |
| MspR9I | CCNGG | 2 cut(s) | 129, 348 |
| MteI | GCGCNGCGC | 1 cut(s) | 24 |
| MunI | CAATTG | 1 cut(s) | 41 |
| MvnI | CGCG | 2 cut(s) | 84, 359 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 32, 291 |
| NciI | CCSGG | 2 cut(s) | 129, 348 |
| NdeI | CATATG | 1 cut(s) | 246 |
| NdeII | GATC | 4 cut(s) | 124, 258, 269, 327 |
| NlaIII | CATG | 2 cut(s) | 111, 306 |
| NlaIV | GGNNCC | 2 cut(s) | 241, 329 |
| NmuCI | GTSAC | 1 cut(s) | 224 |
| NspI | RCATGY | 1 cut(s) | 306 |
| PciSI | GCTCTTC | 1 cut(s) | 356 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PkrI | GCNGC | 3 cut(s) | 25, 68, 322 |
| PleI | GAGTC | 1 cut(s) | 124 |
| PpsI | GAGTC | 1 cut(s) | 124 |
| PspN4I | GGNNCC | 2 cut(s) | 241, 329 |
| PsuI | RGATCY | 2 cut(s) | 124, 327 |
| RsaI | GTAC | 1 cut(s) | 62 |
| RsaNI | GTAC | 1 cut(s) | 61 |
| SapI | GCTCTTC | 1 cut(s) | 356 |
| SaqAI | TTAA | 2 cut(s) | 354, 373 |
| SatI | GCNGC | 3 cut(s) | 24, 67, 321 |
| Sau3AI | GATC | 4 cut(s) | 124, 258, 269, 327 |
| SchI | GAGTC | 1 cut(s) | 125 |
| ScrFI | CCNGG | 2 cut(s) | 129, 348 |
| SduI | GDGCHC | 2 cut(s) | 138, 244 |
| SetI | ASST | 2 cut(s) | 153, 241 |
| SfaNI | GCATC | 1 cut(s) | 294 |
| Sse9I | AATT | 1 cut(s) | 41 |
| SsiI | CCGC | 5 cut(s) | 66, 82, 323, 335, 366 |
| SspMI | CTAG | 1 cut(s) | 332 |
| StyD4I | CCNGG | 2 cut(s) | 127, 346 |
| TaaI | ACNGT | 1 cut(s) | 276 |
| TaqI | TCGA | 4 cut(s) | 99, 119, 140, 207 |
| TasI | AATT | 1 cut(s) | 41 |
| TauI | GCSGC | 1 cut(s) | 69 |
| Tru1I | TTAA | 2 cut(s) | 354, 373 |
| Tru9I | TTAA | 2 cut(s) | 354, 373 |
| TseFI | GTSAC | 1 cut(s) | 224 |
| TseI | GCWGC | 2 cut(s) | 23, 320 |
| Tsp45I | GTSAC | 1 cut(s) | 224 |
| TspDTI | ATGAA | 1 cut(s) | 92 |
| XceI | RCATGY | 1 cut(s) | 306 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |
| XspI | CTAG | 1 cut(s) | 332 |