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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 244 |
| Acc36I | ACCTGC | 2 cut(s) | 244, 391 |
| Acc65I | GGTACC | 2 cut(s) | 376, 403 |
| AccB1I | GGYRCC | 2 cut(s) | 376, 403 |
| AccBSI | CCGCTC | 1 cut(s) | 501 |
| AccI | GTMKAC | 1 cut(s) | 355 |
| AccII | CGCG | 2 cut(s) | 216, 337 |
| AccIII | TCCGGA | 1 cut(s) | 125 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 2 cut(s) | 48, 165 |
| AcoI | YGGCCR | 1 cut(s) | 258 |
| AcsI | RAATTY | 1 cut(s) | 275 |
| AfaI | GTAC | 2 cut(s) | 378, 405 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 199, 253 |
| AgsI | TTSAA | 1 cut(s) | 119 |
| AjnI | CCWGG | 1 cut(s) | 542 |
| AluBI | AGCT | 3 cut(s) | 178, 286, 553 |
| AluI | AGCT | 3 cut(s) | 178, 286, 553 |
| Alw21I | GWGCWC | 2 cut(s) | 113, 344 |
| AlwI | GGATC | 2 cut(s) | 48, 165 |
| Aor13HI | TCCGGA | 1 cut(s) | 125 |
| AoxI | GGCC | 3 cut(s) | 243, 258, 436 |
| ApaI | GGGCCC | 1 cut(s) | 440 |
| ApeKI | GCWGC | 2 cut(s) | 135, 506 |
| ApoI | RAATTY | 1 cut(s) | 275 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 222, 254 |
| Asp718I | GGTACC | 2 cut(s) | 376, 403 |
| AspLEI | GCGC | 3 cut(s) | 216, 228, 298 |
| AspS9I | GGNCC | 7 cut(s) | 128, 190, 244, 436, 437, 513, 578 |
| AvaII | GGWCC | 4 cut(s) | 128, 190, 513, 578 |
| BaeGI | GKGCMC | 1 cut(s) | 440 |
| BalI | TGGCCA | 1 cut(s) | 260 |
| BanI | GGYRCC | 2 cut(s) | 376, 403 |
| BanII | GRGCYC | 1 cut(s) | 440 |
| Bbv12I | GWGCWC | 2 cut(s) | 113, 344 |
| BbvI | GCAGC | 2 cut(s) | 147, 493 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 544 |
| BfaI | CTAG | 4 cut(s) | 89, 149, 510, 608 |
| BfoI | RGCGCY | 1 cut(s) | 299 |
| BfuAI | ACCTGC | 2 cut(s) | 244, 391 |
| BisI | GCNGC | 4 cut(s) | 136, 165, 488, 507 |
| BlsI | GCNGC | 4 cut(s) | 137, 166, 489, 508 |
| Bme1390I | CCNGG | 1 cut(s) | 544 |
| Bme18I | GGWCC | 4 cut(s) | 128, 190, 513, 578 |
| BmgT120I | GGNCC | 7 cut(s) | 128, 190, 244, 436, 437, 513, 578 |
| BmiI | GGNNCC | 4 cut(s) | 378, 405, 438, 580 |
| BmrFI | CCNGG | 1 cut(s) | 544 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 293, 325 |
| BpmI | CTGGAG | 1 cut(s) | 320 |
| Bpu10I | CCTNAGC | 2 cut(s) | 107, 602 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 542 |
| BsaWI | WCCGGW | 1 cut(s) | 125 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 199, 253 |
| Bse1I | ACTGG | 1 cut(s) | 366 |
| BseAI | TCCGGA | 1 cut(s) | 125 |
| BseBI | CCWGG | 1 cut(s) | 544 |
| BseDI | CCNNGG | 2 cut(s) | 72, 542 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 199, 253 |
| BseMII | CTCAG | 1 cut(s) | 98 |
| BseNI | ACTGG | 1 cut(s) | 366 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 1 cut(s) | 440 |
| BseXI | GCAGC | 2 cut(s) | 147, 493 |
| Bsh1236I | CGCG | 2 cut(s) | 216, 337 |
| BshFI | GGCC | 3 cut(s) | 245, 260, 438 |
| BshNI | GGYRCC | 2 cut(s) | 376, 403 |
| BsiHKAI | GWGCWC | 2 cut(s) | 113, 344 |
| BsiSI | CCGG | 1 cut(s) | 126 |
| BslFI | GGGAC | 1 cut(s) | 524 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 199, 253 |
| BsmFI | GGGAC | 1 cut(s) | 524 |
| BsmI | GAATGC | 1 cut(s) | 621 |
| BsnI | GGCC | 3 cut(s) | 245, 260, 438 |
| Bsp120I | GGGCCC | 1 cut(s) | 436 |
| Bsp1286I | GDGCHC | 3 cut(s) | 113, 344, 440 |
| Bsp13I | TCCGGA | 1 cut(s) | 125 |
| Bsp143I | GATC | 2 cut(s) | 40, 157 |
| BspANI | GGCC | 3 cut(s) | 245, 260, 438 |
| BspCNI | CTCAG | 1 cut(s) | 99 |
| BspEI | TCCGGA | 1 cut(s) | 125 |
| BspFNI | CGCG | 2 cut(s) | 216, 337 |
| BspLI | GGNNCC | 4 cut(s) | 378, 405, 438, 580 |
| BspMI | ACCTGC | 2 cut(s) | 244, 391 |
| BspPI | GGATC | 2 cut(s) | 48, 165 |
| BspT107I | GGYRCC | 2 cut(s) | 376, 403 |
| BsrBI | CCGCTC | 1 cut(s) | 501 |
| BsrI | ACTGG | 1 cut(s) | 366 |
| BssECI | CCNNGG | 2 cut(s) | 72, 542 |
| BssMI | GATC | 2 cut(s) | 40, 157 |
| Bst2UI | CCWGG | 1 cut(s) | 544 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 408 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 180, 329 |
| BstDEI | CTNAG | 2 cut(s) | 107, 602 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 2 cut(s) | 216, 337 |
| BstH2I | RGCGCY | 1 cut(s) | 299 |
| BstHHI | GCGC | 3 cut(s) | 216, 228, 298 |
| BstKTI | GATC | 2 cut(s) | 43, 160 |
| BstMBI | GATC | 2 cut(s) | 40, 157 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 302 |
| BstNI | CCWGG | 1 cut(s) | 544 |
| BstSCI | CCNGG | 1 cut(s) | 542 |
| BstSLI | GKGCMC | 1 cut(s) | 440 |
| BstUI | CGCG | 2 cut(s) | 216, 337 |
| BstV1I | GCAGC | 2 cut(s) | 147, 493 |
| BstX2I | RGATCY | 1 cut(s) | 157 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 373 |
| BstYI | RGATCY | 1 cut(s) | 157 |
| BsuRI | GGCC | 3 cut(s) | 245, 260, 438 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 2 cut(s) | 373, 566 |
| BveI | ACCTGC | 2 cut(s) | 244, 391 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 180, 329 |
| CfoI | GCGC | 3 cut(s) | 216, 228, 298 |
| Cfr13I | GGNCC | 7 cut(s) | 128, 190, 244, 436, 437, 513, 578 |
| Csp6I | GTAC | 2 cut(s) | 377, 404 |
| CviAII | CATG | 2 cut(s) | 199, 262 |
| CviQI | GTAC | 2 cut(s) | 377, 404 |
| DdeI | CTNAG | 2 cut(s) | 107, 602 |
| DpnI | GATC | 2 cut(s) | 42, 159 |
| DpnII | GATC | 2 cut(s) | 40, 157 |
| EaeI | YGGCCR | 1 cut(s) | 258 |
| EciI | GGCGGA | 2 cut(s) | 53, 562 |
| Eco24I | GRGCYC | 1 cut(s) | 440 |
| Eco47I | GGWCC | 4 cut(s) | 128, 190, 513, 578 |
| EcoO109I | RGGNCCY | 1 cut(s) | 578 |
| EcoRII | CCWGG | 1 cut(s) | 542 |
| EcoT38I | GRGCYC | 1 cut(s) | 440 |
| FaeI | CATG | 2 cut(s) | 202, 265 |
| FaiI | YATR | 6 cut(s) | 170, 174, 200, 263, 383, 392 |
| FaqI | GGGAC | 1 cut(s) | 524 |
| FatI | CATG | 2 cut(s) | 198, 261 |
| FauI | CCCGC | 1 cut(s) | 589 |
| FblI | GTMKAC | 1 cut(s) | 355 |
| Fnu4HI | GCNGC | 4 cut(s) | 136, 165, 488, 507 |
| FokI | GGATG | 1 cut(s) | 45 |
| FriOI | GRGCYC | 1 cut(s) | 440 |
| Fsp4HI | GCNGC | 4 cut(s) | 136, 165, 488, 507 |
| FspBI | CTAG | 4 cut(s) | 89, 149, 510, 608 |
| GlaI | GCGC | 3 cut(s) | 215, 227, 297 |
| GluI | GCNGC | 4 cut(s) | 136, 165, 488, 507 |
| GsuI | CTGGAG | 1 cut(s) | 320 |
| HaeII | RGCGCY | 1 cut(s) | 299 |
| HaeIII | GGCC | 3 cut(s) | 245, 260, 438 |
| HapII | CCGG | 1 cut(s) | 126 |
| HhaI | GCGC | 3 cut(s) | 216, 228, 298 |
| Hin1II | CATG | 2 cut(s) | 202, 265 |
| Hin6I | GCGC | 3 cut(s) | 214, 226, 296 |
| HinP1I | GCGC | 3 cut(s) | 214, 226, 296 |
| HincII | GTYRAC | 3 cut(s) | 97, 103, 356 |
| HindII | GTYRAC | 3 cut(s) | 97, 103, 356 |
| HinfI | GANTC | 2 cut(s) | 479, 565 |
| HpaI | GTTAAC | 2 cut(s) | 97, 103 |
| HpaII | CCGG | 1 cut(s) | 126 |
| Hpy166II | GTNNAC | 4 cut(s) | 97, 103, 146, 356 |
| Hpy188III | TCNNGA | 1 cut(s) | 126 |
| Hpy8I | GTNNAC | 4 cut(s) | 97, 103, 146, 356 |
| HpyAV | CCTTC | 2 cut(s) | 229, 569 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 408 |
| HpyCH4IV | ACGT | 2 cut(s) | 99, 349 |
| HpyCH4V | TGCA | 2 cut(s) | 400, 619 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 302 |
| HpyF3I | CTNAG | 2 cut(s) | 107, 602 |
| HpySE526I | ACGT | 2 cut(s) | 99, 349 |
| Hsp92II | CATG | 2 cut(s) | 202, 265 |
| HspAI | GCGC | 3 cut(s) | 214, 226, 296 |
| Kpn2I | TCCGGA | 1 cut(s) | 125 |
| KpnI | GGTACC | 2 cut(s) | 380, 407 |
| KspAI | GTTAAC | 2 cut(s) | 97, 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 157 |
| LmnI | GCTCC | 2 cut(s) | 31, 339 |
| Lsp1109I | GCAGC | 2 cut(s) | 147, 493 |
| MaeI | CTAG | 4 cut(s) | 89, 149, 510, 608 |
| MaeII | ACGT | 2 cut(s) | 99, 349 |
| MaeIII | GTNAC | 1 cut(s) | 480 |
| MalI | GATC | 2 cut(s) | 42, 159 |
| MbiI | CCGCTC | 1 cut(s) | 501 |
| MboI | GATC | 2 cut(s) | 40, 157 |
| MflI | RGATCY | 1 cut(s) | 157 |
| MhlI | GDGCHC | 3 cut(s) | 113, 344, 440 |
| MlsI | TGGCCA | 1 cut(s) | 260 |
| MluCI | AATT | 1 cut(s) | 275 |
| MluNI | TGGCCA | 1 cut(s) | 260 |
| MlyI | GAGTC | 1 cut(s) | 488 |
| MnlI | CCTC | 8 cut(s) | 8, 22, 82, 203, 235, 295, 453, 517 |
| Mox20I | TGGCCA | 1 cut(s) | 260 |
| MroI | TCCGGA | 1 cut(s) | 125 |
| MscI | TGGCCA | 1 cut(s) | 260 |
| MseI | TTAA | 2 cut(s) | 96, 102 |
| MslI | CAYNNNNRTG | 2 cut(s) | 266, 395 |
| Msp20I | TGGCCA | 1 cut(s) | 260 |
| MspA1I | CMGCKG | 1 cut(s) | 584 |
| MspI | CCGG | 1 cut(s) | 126 |
| MspR9I | CCNGG | 1 cut(s) | 544 |
| Mva1269I | GAATGC | 1 cut(s) | 621 |
| MvaI | CCWGG | 1 cut(s) | 544 |
| MvnI | CGCG | 2 cut(s) | 216, 337 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 302 |
| NdeII | GATC | 2 cut(s) | 40, 157 |
| NlaIII | CATG | 2 cut(s) | 202, 265 |
| NlaIV | GGNNCC | 4 cut(s) | 378, 405, 438, 580 |
| NmeAIII | GCCGAG | 2 cut(s) | 53, 482 |
| NmuCI | GTSAC | 1 cut(s) | 480 |
| PaqCI | CACCTGC | 1 cut(s) | 244 |
| PctI | GAATGC | 1 cut(s) | 621 |
| PfeI | GAWTC | 1 cut(s) | 565 |
| PflFI | GACNNNGTC | 1 cut(s) | 351 |
| PkrI | GCNGC | 4 cut(s) | 137, 166, 489, 508 |
| PleI | GAGTC | 1 cut(s) | 487 |
| PpsI | GAGTC | 1 cut(s) | 487 |
| PpuMI | RGGWCCY | 1 cut(s) | 578 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 578 |
| Psp6I | CCWGG | 1 cut(s) | 542 |
| PspGI | CCWGG | 1 cut(s) | 542 |
| PspN4I | GGNNCC | 4 cut(s) | 378, 405, 438, 580 |
| PspOMI | GGGCCC | 1 cut(s) | 436 |
| PspPI | GGNCC | 7 cut(s) | 128, 190, 244, 436, 437, 513, 578 |
| PspPPI | RGGWCCY | 1 cut(s) | 578 |
| PsuI | RGATCY | 1 cut(s) | 157 |
| PsyI | GACNNNGTC | 1 cut(s) | 351 |
| RsaI | GTAC | 2 cut(s) | 378, 405 |
| RsaNI | GTAC | 2 cut(s) | 377, 404 |
| RseI | CAYNNNNRTG | 2 cut(s) | 266, 395 |
| SalI | GTCGAC | 1 cut(s) | 354 |
| SaqAI | TTAA | 2 cut(s) | 96, 102 |
| SatI | GCNGC | 4 cut(s) | 136, 165, 488, 507 |
| Sau3AI | GATC | 2 cut(s) | 40, 157 |
| Sau96I | GGNCC | 7 cut(s) | 128, 190, 244, 436, 437, 513, 578 |
| SchI | GAGTC | 1 cut(s) | 488 |
| ScrFI | CCNGG | 1 cut(s) | 544 |
| SduI | GDGCHC | 3 cut(s) | 113, 344, 440 |
| SinI | GGWCC | 4 cut(s) | 128, 190, 513, 578 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 266, 395 |
| Sse9I | AATT | 1 cut(s) | 275 |
| SspMI | CTAG | 4 cut(s) | 89, 149, 510, 608 |
| StyD4I | CCNGG | 1 cut(s) | 542 |
| TaaI | ACNGT | 2 cut(s) | 11, 408 |
| TaiI | ACGT | 2 cut(s) | 102, 352 |
| TaqI | TCGA | 3 cut(s) | 240, 279, 355 |
| TasI | AATT | 1 cut(s) | 275 |
| TauI | GCSGC | 2 cut(s) | 167, 490 |
| TfiI | GAWTC | 1 cut(s) | 565 |
| Tru1I | TTAA | 2 cut(s) | 96, 102 |
| Tru9I | TTAA | 2 cut(s) | 96, 102 |
| TscAI | CASTG | 2 cut(s) | 373, 566 |
| TseFI | GTSAC | 1 cut(s) | 480 |
| TseI | GCWGC | 2 cut(s) | 135, 506 |
| Tsp45I | GTSAC | 1 cut(s) | 480 |
| TspRI | CASTG | 2 cut(s) | 373, 566 |
| Tth111I | GACNNNGTC | 1 cut(s) | 351 |
| VpaK11BI | GGWCC | 4 cut(s) | 128, 190, 513, 578 |
| XapI | RAATTY | 1 cut(s) | 275 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 550 |
| XmiI | GTMKAC | 1 cut(s) | 355 |
| XspI | CTAG | 4 cut(s) | 89, 149, 510, 608 |