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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 374 |
| AccII | CGCG | 3 cut(s) | 51, 192, 550 |
| AciI | CCGC | 4 cut(s) | 192, 198, 402, 476 |
| AclWI | GGATC | 1 cut(s) | 402 |
| AcoI | YGGCCR | 1 cut(s) | 399 |
| AcsI | RAATTY | 2 cut(s) | 70, 512 |
| AfaI | GTAC | 1 cut(s) | 689 |
| AfeI | AGCGCT | 1 cut(s) | 534 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 73, 397, 599, 652 |
| AjnI | CCWGG | 2 cut(s) | 28, 651 |
| AluBI | AGCT | 4 cut(s) | 106, 230, 375, 523 |
| AluI | AGCT | 4 cut(s) | 106, 230, 375, 523 |
| Alw26I | GTCTC | 1 cut(s) | 127 |
| AlwI | GGATC | 1 cut(s) | 402 |
| Ama87I | CYCGRG | 1 cut(s) | 326 |
| Aor51HI | AGCGCT | 1 cut(s) | 534 |
| AoxI | GGCC | 4 cut(s) | 388, 399, 584, 643 |
| ApeKI | GCWGC | 1 cut(s) | 611 |
| ApoI | RAATTY | 2 cut(s) | 70, 512 |
| AspLEI | GCGC | 5 cut(s) | 53, 192, 385, 535, 550 |
| AspS9I | GGNCC | 1 cut(s) | 585 |
| AsuC2I | CCSGG | 1 cut(s) | 392 |
| AsuHPI | GGTGA | 2 cut(s) | 252, 277 |
| AvaI | CYCGRG | 1 cut(s) | 326 |
| BaeGI | GKGCMC | 1 cut(s) | 209 |
| BbvCI | CCTCAGC | 1 cut(s) | 240 |
| BbvI | GCAGC | 1 cut(s) | 623 |
| BceAI | ACGGC | 1 cut(s) | 203 |
| BciT130I | CCWGG | 2 cut(s) | 30, 653 |
| BcnI | CCSGG | 1 cut(s) | 392 |
| BcoDI | GTCTC | 1 cut(s) | 127 |
| BfaI | CTAG | 2 cut(s) | 536, 662 |
| BfmI | CTRYAG | 1 cut(s) | 80 |
| BfoI | RGCGCY | 1 cut(s) | 536 |
| BfuAI | ACCTGC | 1 cut(s) | 374 |
| BisI | GCNGC | 3 cut(s) | 199, 402, 612 |
| BlsI | GCNGC | 3 cut(s) | 200, 403, 613 |
| Bme1390I | CCNGG | 3 cut(s) | 30, 392, 653 |
| BmeT110I | CYCGRG | 1 cut(s) | 326 |
| BmgT120I | GGNCC | 1 cut(s) | 585 |
| BmrFI | CCNGG | 3 cut(s) | 30, 392, 653 |
| BmrI | ACTGGG | 1 cut(s) | 679 |
| BmuI | ACTGGG | 1 cut(s) | 679 |
| BoxI | GACNNNNGTC | 1 cut(s) | 157 |
| BpmI | CTGGAG | 1 cut(s) | 12 |
| Bpu10I | CCTNAGC | 1 cut(s) | 240 |
| BpuMI | CCSGG | 1 cut(s) | 392 |
| BsaJI | CCNNGG | 2 cut(s) | 327, 420 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 73, 397, 599, 652 |
| Bse1I | ACTGG | 2 cut(s) | 214, 685 |
| BseBI | CCWGG | 2 cut(s) | 30, 653 |
| BseDI | CCNNGG | 2 cut(s) | 327, 420 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 73, 397, 599, 652 |
| BseMII | CTCAG | 4 cut(s) | 98, 112, 138, 254 |
| BseNI | ACTGG | 2 cut(s) | 214, 685 |
| BseSI | GKGCMC | 1 cut(s) | 209 |
| BseXI | GCAGC | 1 cut(s) | 623 |
| BseYI | CCCAGC | 1 cut(s) | 587 |
| Bsh1236I | CGCG | 3 cut(s) | 51, 192, 550 |
| BshFI | GGCC | 4 cut(s) | 390, 401, 586, 645 |
| BsiHKCI | CYCGRG | 1 cut(s) | 326 |
| BsiSI | CCGG | 1 cut(s) | 391 |
| BslFI | GGGAC | 1 cut(s) | 616 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 73, 397, 599, 652 |
| BsmAI | GTCTC | 1 cut(s) | 127 |
| BsmFI | GGGAC | 1 cut(s) | 616 |
| BsmI | GAATGC | 1 cut(s) | 332 |
| BsnI | GGCC | 4 cut(s) | 390, 401, 586, 645 |
| BsoBI | CYCGRG | 1 cut(s) | 326 |
| Bsp1286I | GDGCHC | 1 cut(s) | 209 |
| Bsp143I | GATC | 2 cut(s) | 223, 394 |
| BspACI | CCGC | 4 cut(s) | 192, 198, 402, 476 |
| BspANI | GGCC | 4 cut(s) | 390, 401, 586, 645 |
| BspCNI | CTCAG | 4 cut(s) | 99, 111, 137, 253 |
| BspFNI | CGCG | 3 cut(s) | 51, 192, 550 |
| BspMAI | CTGCAG | 1 cut(s) | 84 |
| BspMI | ACCTGC | 1 cut(s) | 374 |
| BspPI | GGATC | 1 cut(s) | 402 |
| BsrI | ACTGG | 2 cut(s) | 214, 685 |
| BssECI | CCNNGG | 2 cut(s) | 327, 420 |
| BssMI | GATC | 2 cut(s) | 223, 394 |
| BssT1I | CCWWGG | 1 cut(s) | 420 |
| Bst2UI | CCWGG | 2 cut(s) | 30, 653 |
| Bst4CI | ACNGT | 3 cut(s) | 268, 275, 282 |
| BstC8I | GCNNGC | 1 cut(s) | 104 |
| BstDEI | CTNAG | 4 cut(s) | 98, 107, 124, 240 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 650 |
| BstFNI | CGCG | 3 cut(s) | 51, 192, 550 |
| BstH2I | RGCGCY | 1 cut(s) | 536 |
| BstHHI | GCGC | 5 cut(s) | 53, 192, 385, 535, 550 |
| BstKTI | GATC | 2 cut(s) | 226, 397 |
| BstMAI | GTCTC | 1 cut(s) | 127 |
| BstMBI | GATC | 2 cut(s) | 223, 394 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 198, 204, 662 |
| BstNI | CCWGG | 2 cut(s) | 30, 653 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 157 |
| BstSCI | CCNGG | 3 cut(s) | 28, 390, 651 |
| BstSFI | CTRYAG | 1 cut(s) | 80 |
| BstSLI | GKGCMC | 1 cut(s) | 209 |
| BstUI | CGCG | 3 cut(s) | 51, 192, 550 |
| BstV1I | GCAGC | 1 cut(s) | 623 |
| BstX2I | RGATCY | 1 cut(s) | 394 |
| BstYI | RGATCY | 1 cut(s) | 394 |
| BsuRI | GGCC | 4 cut(s) | 390, 401, 586, 645 |
| BtsIMutI | CAGTG | 1 cut(s) | 207 |
| BveI | ACCTGC | 1 cut(s) | 374 |
| Cac8I | GCNNGC | 1 cut(s) | 104 |
| CfoI | GCGC | 5 cut(s) | 53, 192, 385, 535, 550 |
| Cfr13I | GGNCC | 1 cut(s) | 585 |
| CseI | GACGC | 1 cut(s) | 508 |
| Csp6I | GTAC | 1 cut(s) | 688 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 282, 317 |
| CviQI | GTAC | 1 cut(s) | 688 |
| DdeI | CTNAG | 4 cut(s) | 98, 107, 124, 240 |
| DpnI | GATC | 2 cut(s) | 225, 396 |
| DpnII | GATC | 2 cut(s) | 223, 394 |
| EaeI | YGGCCR | 1 cut(s) | 399 |
| EciI | GGCGGA | 1 cut(s) | 491 |
| Eco130I | CCWWGG | 1 cut(s) | 420 |
| Eco47III | AGCGCT | 1 cut(s) | 534 |
| Eco88I | CYCGRG | 1 cut(s) | 326 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 650 |
| EcoRII | CCWGG | 2 cut(s) | 28, 651 |
| EcoT14I | CCWWGG | 1 cut(s) | 420 |
| ErhI | CCWWGG | 1 cut(s) | 420 |
| FaiI | YATR | 4 cut(s) | 133, 139, 371, 407 |
| FaqI | GGGAC | 1 cut(s) | 616 |
| Fnu4HI | GCNGC | 3 cut(s) | 199, 402, 612 |
| Fsp4HI | GCNGC | 3 cut(s) | 199, 402, 612 |
| FspBI | CTAG | 2 cut(s) | 536, 662 |
| GlaI | GCGC | 5 cut(s) | 52, 191, 384, 534, 549 |
| GluI | GCNGC | 3 cut(s) | 199, 402, 612 |
| GsaI | CCCAGC | 1 cut(s) | 591 |
| GsuI | CTGGAG | 1 cut(s) | 12 |
| HaeII | RGCGCY | 1 cut(s) | 536 |
| HaeIII | GGCC | 4 cut(s) | 390, 401, 586, 645 |
| HapII | CCGG | 1 cut(s) | 391 |
| HgaI | GACGC | 1 cut(s) | 508 |
| HhaI | GCGC | 5 cut(s) | 53, 192, 385, 535, 550 |
| Hin6I | GCGC | 5 cut(s) | 51, 190, 383, 533, 548 |
| HinP1I | GCGC | 5 cut(s) | 51, 190, 383, 533, 548 |
| HincII | GTYRAC | 2 cut(s) | 24, 271 |
| HindII | GTYRAC | 2 cut(s) | 24, 271 |
| HinfI | GANTC | 4 cut(s) | 120, 153, 179, 313 |
| HpaII | CCGG | 1 cut(s) | 391 |
| HphI | GGTGA | 2 cut(s) | 252, 277 |
| Hpy166II | GTNNAC | 7 cut(s) | 24, 162, 271, 278, 285, 304, 566 |
| Hpy188I | TCNGA | 4 cut(s) | 127, 144, 172, 363 |
| Hpy8I | GTNNAC | 7 cut(s) | 24, 162, 271, 278, 285, 304, 566 |
| Hpy99I | CGWCG | 1 cut(s) | 502 |
| HpyAV | CCTTC | 1 cut(s) | 466 |
| HpyCH4III | ACNGT | 3 cut(s) | 268, 275, 282 |
| HpyCH4IV | ACGT | 1 cut(s) | 300 |
| HpyCH4V | TGCA | 3 cut(s) | 82, 369, 581 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 198, 204, 662 |
| HpyF3I | CTNAG | 4 cut(s) | 98, 107, 124, 240 |
| HpySE526I | ACGT | 1 cut(s) | 300 |
| HspAI | GCGC | 5 cut(s) | 51, 190, 383, 533, 548 |
| Kzo9I | GATC | 2 cut(s) | 223, 394 |
| Lsp1109I | GCAGC | 1 cut(s) | 623 |
| MaeI | CTAG | 2 cut(s) | 536, 662 |
| MaeII | ACGT | 1 cut(s) | 300 |
| MalI | GATC | 2 cut(s) | 225, 396 |
| MboI | GATC | 2 cut(s) | 223, 394 |
| MboII | GAAGA | 1 cut(s) | 157 |
| MflI | RGATCY | 1 cut(s) | 394 |
| MhlI | GDGCHC | 1 cut(s) | 209 |
| MluCI | AATT | 3 cut(s) | 70, 294, 512 |
| MlyI | GAGTC | 3 cut(s) | 129, 147, 173 |
| MmeI | TCCRAC | 1 cut(s) | 390 |
| MnlI | CCTC | 5 cut(s) | 19, 54, 196, 249, 322 |
| MseI | TTAA | 2 cut(s) | 113, 297 |
| MspI | CCGG | 1 cut(s) | 391 |
| MspR9I | CCNGG | 3 cut(s) | 30, 392, 653 |
| Mva1269I | GAATGC | 1 cut(s) | 332 |
| MvaI | CCWGG | 2 cut(s) | 30, 653 |
| MvnI | CGCG | 3 cut(s) | 51, 192, 550 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 198, 204, 662 |
| NciI | CCSGG | 1 cut(s) | 392 |
| NdeII | GATC | 2 cut(s) | 223, 394 |
| PctI | GAATGC | 1 cut(s) | 332 |
| PfeI | GAWTC | 1 cut(s) | 313 |
| PkrI | GCNGC | 3 cut(s) | 200, 403, 613 |
| PleI | GAGTC | 3 cut(s) | 128, 147, 173 |
| PpsI | GAGTC | 3 cut(s) | 128, 147, 173 |
| PshAI | GACNNNNGTC | 1 cut(s) | 157 |
| Psp6I | CCWGG | 2 cut(s) | 28, 651 |
| PspFI | CCCAGC | 1 cut(s) | 587 |
| PspGI | CCWGG | 2 cut(s) | 28, 651 |
| PspPI | GGNCC | 1 cut(s) | 585 |
| PstI | CTGCAG | 1 cut(s) | 84 |
| PsuI | RGATCY | 1 cut(s) | 394 |
| RsaI | GTAC | 1 cut(s) | 689 |
| RsaNI | GTAC | 1 cut(s) | 688 |
| SaqAI | TTAA | 2 cut(s) | 113, 297 |
| SatI | GCNGC | 3 cut(s) | 199, 402, 612 |
| Sau3AI | GATC | 2 cut(s) | 223, 394 |
| Sau96I | GGNCC | 1 cut(s) | 585 |
| SchI | GAGTC | 3 cut(s) | 129, 147, 173 |
| ScrFI | CCNGG | 3 cut(s) | 30, 392, 653 |
| SduI | GDGCHC | 1 cut(s) | 209 |
| SetI | ASST | 8 cut(s) | 11, 108, 232, 303, 368, 377, 525, 640 |
| SfcI | CTRYAG | 1 cut(s) | 80 |
| Sse9I | AATT | 3 cut(s) | 70, 294, 512 |
| SsiI | CCGC | 4 cut(s) | 192, 198, 402, 476 |
| SspMI | CTAG | 2 cut(s) | 536, 662 |
| StyD4I | CCNGG | 3 cut(s) | 28, 390, 651 |
| StyI | CCWWGG | 1 cut(s) | 420 |
| TaaI | ACNGT | 3 cut(s) | 268, 275, 282 |
| TaiI | ACGT | 1 cut(s) | 303 |
| TasI | AATT | 3 cut(s) | 70, 294, 512 |
| TatI | WGTACW | 1 cut(s) | 687 |
| TauI | GCSGC | 2 cut(s) | 201, 404 |
| TfiI | GAWTC | 1 cut(s) | 313 |
| Tru1I | TTAA | 2 cut(s) | 113, 297 |
| Tru9I | TTAA | 2 cut(s) | 113, 297 |
| TscAI | CASTG | 1 cut(s) | 214 |
| TseI | GCWGC | 1 cut(s) | 611 |
| TspGWI | ACGGA | 2 cut(s) | 321, 337 |
| TspRI | CASTG | 1 cut(s) | 214 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 650 |
| XapI | RAATTY | 2 cut(s) | 70, 512 |
| XspI | CTAG | 2 cut(s) | 536, 662 |