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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 316 |
| Acc36I | ACCTGC | 1 cut(s) | 316 |
| AciI | CCGC | 3 cut(s) | 21, 54, 191 |
| AclI | AACGTT | 1 cut(s) | 105 |
| AclWI | GGATC | 2 cut(s) | 45, 163 |
| AcoI | YGGCCR | 1 cut(s) | 330 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 271, 325 |
| AgsI | TTSAA | 1 cut(s) | 124 |
| AjnI | CCWGG | 3 cut(s) | 153, 220, 225 |
| AluBI | AGCT | 2 cut(s) | 250, 298 |
| AluI | AGCT | 2 cut(s) | 250, 298 |
| Alw21I | GWGCWC | 2 cut(s) | 118, 300 |
| Alw44I | GTGCAC | 1 cut(s) | 114 |
| AlwI | GGATC | 2 cut(s) | 45, 163 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 172 |
| AoxI | GGCC | 3 cut(s) | 223, 309, 330 |
| ApaLI | GTGCAC | 1 cut(s) | 114 |
| ApeKI | GCWGC | 1 cut(s) | 207 |
| AspS9I | GGNCC | 1 cut(s) | 229 |
| AvaII | GGWCC | 1 cut(s) | 229 |
| BaeGI | GKGCMC | 1 cut(s) | 118 |
| BalI | TGGCCA | 1 cut(s) | 332 |
| BanII | GRGCYC | 1 cut(s) | 300 |
| Bbv12I | GWGCWC | 2 cut(s) | 118, 300 |
| BbvI | GCAGC | 1 cut(s) | 219 |
| BccI | CCATC | 1 cut(s) | 103 |
| BciT130I | CCWGG | 3 cut(s) | 155, 222, 227 |
| BfmI | CTRYAG | 1 cut(s) | 186 |
| BfuAI | ACCTGC | 1 cut(s) | 316 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 222 |
| BisI | GCNGC | 1 cut(s) | 208 |
| BlsI | GCNGC | 1 cut(s) | 209 |
| Bme1390I | CCNGG | 3 cut(s) | 155, 222, 227 |
| Bme18I | GGWCC | 1 cut(s) | 229 |
| BmgT120I | GGNCC | 1 cut(s) | 229 |
| BmrFI | CCNGG | 3 cut(s) | 155, 222, 227 |
| BmrI | ACTGGG | 1 cut(s) | 135 |
| BmuI | ACTGGG | 1 cut(s) | 135 |
| BpmI | CTGGAG | 1 cut(s) | 392 |
| BsaJI | CCNNGG | 1 cut(s) | 153 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 221, 251 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 271, 325 |
| Bse1I | ACTGG | 3 cut(s) | 141, 261, 375 |
| BseBI | CCWGG | 3 cut(s) | 155, 222, 227 |
| BseDI | CCNNGG | 1 cut(s) | 153 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 271, 325 |
| BseNI | ACTGG | 3 cut(s) | 141, 261, 375 |
| BseSI | GKGCMC | 1 cut(s) | 118 |
| BseXI | GCAGC | 1 cut(s) | 219 |
| BseYI | CCCAGC | 1 cut(s) | 388 |
| BshFI | GGCC | 3 cut(s) | 225, 311, 332 |
| BsiHKAI | GWGCWC | 2 cut(s) | 118, 300 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 271, 325 |
| BsnI | GGCC | 3 cut(s) | 225, 311, 332 |
| Bsp1286I | GDGCHC | 2 cut(s) | 118, 300 |
| Bsp143I | GATC | 2 cut(s) | 50, 168 |
| BspACI | CCGC | 3 cut(s) | 21, 54, 191 |
| BspANI | GGCC | 3 cut(s) | 225, 311, 332 |
| BspMI | ACCTGC | 1 cut(s) | 316 |
| BspPI | GGATC | 2 cut(s) | 45, 163 |
| BspQI | GCTCTTC | 1 cut(s) | 30 |
| BsrI | ACTGG | 3 cut(s) | 141, 261, 375 |
| BssECI | CCNNGG | 1 cut(s) | 153 |
| BssMI | GATC | 2 cut(s) | 50, 168 |
| Bst2UI | CCWGG | 3 cut(s) | 155, 222, 227 |
| Bst6I | CTCTTC | 1 cut(s) | 30 |
| BstC8I | GCNNGC | 2 cut(s) | 42, 252 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 269 |
| BstKTI | GATC | 2 cut(s) | 53, 171 |
| BstMBI | GATC | 2 cut(s) | 50, 168 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 213, 222, 374 |
| BstNI | CCWGG | 3 cut(s) | 155, 222, 227 |
| BstSCI | CCNGG | 3 cut(s) | 153, 220, 225 |
| BstSFI | CTRYAG | 1 cut(s) | 186 |
| BstSLI | GKGCMC | 1 cut(s) | 118 |
| BstV1I | GCAGC | 1 cut(s) | 219 |
| BstX2I | RGATCY | 1 cut(s) | 168 |
| BstYI | RGATCY | 1 cut(s) | 168 |
| BsuRI | GGCC | 3 cut(s) | 225, 311, 332 |
| BtsIMutI | CAGTG | 3 cut(s) | 148, 254, 368 |
| BveI | ACCTGC | 1 cut(s) | 316 |
| Cac8I | GCNNGC | 2 cut(s) | 42, 252 |
| CaiI | CAGNNNCTG | 1 cut(s) | 172 |
| Cfr13I | GGNCC | 1 cut(s) | 229 |
| CviAII | CATG | 4 cut(s) | 7, 241, 301, 334 |
| CviJI | RGCY | 7 cut(s) | 197, 225, 250, 298, 311, 317, 332 |
| CviKI_1 | RGCY | 7 cut(s) | 197, 225, 250, 298, 311, 317, 332 |
| DpnI | GATC | 2 cut(s) | 52, 170 |
| DpnII | GATC | 2 cut(s) | 50, 168 |
| EaeI | YGGCCR | 1 cut(s) | 330 |
| Eam1104I | CTCTTC | 1 cut(s) | 30 |
| EarI | CTCTTC | 1 cut(s) | 30 |
| EciI | GGCGGA | 1 cut(s) | 43 |
| Ecl136II | GAGCTC | 1 cut(s) | 298 |
| Eco24I | GRGCYC | 1 cut(s) | 300 |
| Eco47I | GGWCC | 1 cut(s) | 229 |
| Eco53kI | GAGCTC | 1 cut(s) | 298 |
| EcoICRI | GAGCTC | 1 cut(s) | 298 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 269 |
| EcoO109I | RGGNCCY | 1 cut(s) | 229 |
| EcoRII | CCWGG | 3 cut(s) | 153, 220, 225 |
| EcoT38I | GRGCYC | 1 cut(s) | 300 |
| FaeI | CATG | 4 cut(s) | 10, 244, 304, 337 |
| FaiI | YATR | 5 cut(s) | 8, 242, 246, 302, 335 |
| FatI | CATG | 4 cut(s) | 6, 240, 300, 333 |
| FauI | CCCGC | 1 cut(s) | 14 |
| Fnu4HI | GCNGC | 1 cut(s) | 208 |
| FriOI | GRGCYC | 1 cut(s) | 300 |
| Fsp4HI | GCNGC | 1 cut(s) | 208 |
| GluI | GCNGC | 1 cut(s) | 208 |
| GsaI | CCCAGC | 1 cut(s) | 392 |
| GsuI | CTGGAG | 1 cut(s) | 392 |
| HaeIII | GGCC | 3 cut(s) | 225, 311, 332 |
| Hin1II | CATG | 4 cut(s) | 10, 244, 304, 337 |
| HincII | GTYRAC | 1 cut(s) | 103 |
| HindII | GTYRAC | 1 cut(s) | 103 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| Hpy166II | GTNNAC | 2 cut(s) | 103, 116 |
| Hpy8I | GTNNAC | 2 cut(s) | 103, 116 |
| Hpy99I | CGWCG | 2 cut(s) | 35, 356 |
| HpyAV | CCTTC | 2 cut(s) | 301, 358 |
| HpyCH4IV | ACGT | 2 cut(s) | 30, 105 |
| HpyCH4V | TGCA | 1 cut(s) | 116 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 213, 222, 374 |
| HpySE526I | ACGT | 2 cut(s) | 30, 105 |
| Hsp92II | CATG | 4 cut(s) | 10, 244, 304, 337 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 2 cut(s) | 50, 168 |
| LguI | GCTCTTC | 1 cut(s) | 30 |
| Lsp1109I | GCAGC | 1 cut(s) | 219 |
| MaeII | ACGT | 2 cut(s) | 30, 105 |
| MalI | GATC | 2 cut(s) | 52, 170 |
| MboI | GATC | 2 cut(s) | 50, 168 |
| MboII | GAAGA | 1 cut(s) | 47 |
| MflI | RGATCY | 1 cut(s) | 168 |
| MhlI | GDGCHC | 2 cut(s) | 118, 300 |
| MlsI | TGGCCA | 1 cut(s) | 332 |
| MluNI | TGGCCA | 1 cut(s) | 332 |
| MnlI | CCTC | 5 cut(s) | 187, 242, 247, 275, 367 |
| Mox20I | TGGCCA | 1 cut(s) | 332 |
| MscI | TGGCCA | 1 cut(s) | 332 |
| MseI | TTAA | 3 cut(s) | 90, 102, 108 |
| MslI | CAYNNNNRTG | 1 cut(s) | 338 |
| Msp20I | TGGCCA | 1 cut(s) | 332 |
| MspA1I | CMGCKG | 1 cut(s) | 191 |
| MspR9I | CCNGG | 3 cut(s) | 155, 222, 227 |
| MvaI | CCWGG | 3 cut(s) | 155, 222, 227 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 213, 222, 374 |
| NdeII | GATC | 2 cut(s) | 50, 168 |
| NlaIII | CATG | 4 cut(s) | 10, 244, 304, 337 |
| PaqCI | CACCTGC | 1 cut(s) | 316 |
| PciSI | GCTCTTC | 1 cut(s) | 30 |
| PkrI | GCNGC | 1 cut(s) | 209 |
| PpuMI | RGGWCCY | 1 cut(s) | 229 |
| Psp124BI | GAGCTC | 1 cut(s) | 300 |
| Psp1406I | AACGTT | 1 cut(s) | 105 |
| Psp5II | RGGWCCY | 1 cut(s) | 229 |
| Psp6I | CCWGG | 3 cut(s) | 153, 220, 225 |
| PspFI | CCCAGC | 1 cut(s) | 388 |
| PspGI | CCWGG | 3 cut(s) | 153, 220, 225 |
| PspPI | GGNCC | 1 cut(s) | 229 |
| PspPPI | RGGWCCY | 1 cut(s) | 229 |
| PstNI | CAGNNNCTG | 1 cut(s) | 172 |
| PsuI | RGATCY | 1 cut(s) | 168 |
| RseI | CAYNNNNRTG | 1 cut(s) | 338 |
| SacI | GAGCTC | 1 cut(s) | 300 |
| SapI | GCTCTTC | 1 cut(s) | 30 |
| SaqAI | TTAA | 3 cut(s) | 90, 102, 108 |
| SatI | GCNGC | 1 cut(s) | 208 |
| Sau3AI | GATC | 2 cut(s) | 50, 168 |
| Sau96I | GGNCC | 1 cut(s) | 229 |
| ScrFI | CCNGG | 3 cut(s) | 155, 222, 227 |
| SduI | GDGCHC | 2 cut(s) | 118, 300 |
| SetI | ASST | 8 cut(s) | 33, 108, 234, 252, 267, 300, 330, 350 |
| SfcI | CTRYAG | 1 cut(s) | 186 |
| SinI | GGWCC | 1 cut(s) | 229 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 338 |
| SsiI | CCGC | 3 cut(s) | 21, 54, 191 |
| SstI | GAGCTC | 1 cut(s) | 300 |
| StyD4I | CCNGG | 3 cut(s) | 153, 220, 225 |
| TaiI | ACGT | 2 cut(s) | 33, 108 |
| TaqI | TCGA | 3 cut(s) | 33, 199, 351 |
| Tru1I | TTAA | 3 cut(s) | 90, 102, 108 |
| Tru9I | TTAA | 3 cut(s) | 90, 102, 108 |
| TscAI | CASTG | 3 cut(s) | 148, 261, 375 |
| TseI | GCWGC | 1 cut(s) | 207 |
| TspRI | CASTG | 3 cut(s) | 148, 261, 375 |
| VneI | GTGCAC | 1 cut(s) | 114 |
| VpaK11BI | GGWCC | 1 cut(s) | 229 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 269 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 247 |