No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 142 |
| Acc36I | ACCTGC | 1 cut(s) | 142 |
| AccBSI | CCGCTC | 2 cut(s) | 399, 496 |
| AciI | CCGC | 6 cut(s) | 15, 86, 305, 338, 397, 494 |
| AclWI | GGATC | 2 cut(s) | 96, 456 |
| AcoI | YGGCCR | 1 cut(s) | 156 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 1 cut(s) | 262 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 76, 151, 263, 445 |
| AhlI | ACTAGT | 1 cut(s) | 283 |
| AjnI | CCWGG | 1 cut(s) | 242 |
| AleI | CACNNNNGTG | 1 cut(s) | 164 |
| AluBI | AGCT | 1 cut(s) | 322 |
| AluI | AGCT | 1 cut(s) | 322 |
| Alw21I | GWGCWC | 1 cut(s) | 242 |
| Alw26I | GTCTC | 1 cut(s) | 397 |
| AlwI | GGATC | 2 cut(s) | 96, 456 |
| Ama87I | CYCGRG | 1 cut(s) | 400 |
| AoxI | GGCC | 4 cut(s) | 141, 156, 335, 469 |
| AspLEI | GCGC | 3 cut(s) | 76, 126, 269 |
| AspS9I | GGNCC | 5 cut(s) | 142, 301, 310, 335, 470 |
| AsuC2I | CCSGG | 3 cut(s) | 71, 215, 468 |
| AvaI | CYCGRG | 1 cut(s) | 400 |
| AvaII | GGWCC | 2 cut(s) | 301, 310 |
| BaeGI | GKGCMC | 1 cut(s) | 351 |
| BalI | TGGCCA | 1 cut(s) | 158 |
| Bbv12I | GWGCWC | 1 cut(s) | 242 |
| BbvCI | CCTCAGC | 1 cut(s) | 138 |
| BceAI | ACGGC | 1 cut(s) | 114 |
| BciT130I | CCWGG | 1 cut(s) | 244 |
| BcnI | CCSGG | 3 cut(s) | 71, 215, 468 |
| BcoDI | GTCTC | 1 cut(s) | 397 |
| BcuI | ACTAGT | 1 cut(s) | 283 |
| BfaI | CTAG | 4 cut(s) | 258, 284, 317, 456 |
| BfoI | RGCGCY | 1 cut(s) | 77 |
| BfuAI | ACCTGC | 1 cut(s) | 142 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 215, 482 |
| BisI | GCNGC | 1 cut(s) | 338 |
| BlsI | GCNGC | 1 cut(s) | 339 |
| Bme1390I | CCNGG | 4 cut(s) | 71, 215, 244, 468 |
| Bme18I | GGWCC | 2 cut(s) | 301, 310 |
| BmeT110I | CYCGRG | 1 cut(s) | 400 |
| BmgT120I | GGNCC | 5 cut(s) | 142, 301, 310, 335, 470 |
| BmiI | GGNNCC | 3 cut(s) | 303, 312, 336 |
| BmrFI | CCNGG | 4 cut(s) | 71, 215, 244, 468 |
| BmrI | ACTGGG | 1 cut(s) | 346 |
| BmsI | GCATC | 1 cut(s) | 477 |
| BmuI | ACTGGG | 1 cut(s) | 346 |
| BpmI | CTGGAG | 1 cut(s) | 218 |
| Bpu10I | CCTNAGC | 1 cut(s) | 138 |
| BpuMI | CCSGG | 3 cut(s) | 71, 215, 468 |
| BsaI | GGTCTC | 1 cut(s) | 397 |
| BsaJI | CCNNGG | 2 cut(s) | 407, 420 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 517 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 76, 151, 263, 445 |
| Bse118I | RCCGGY | 1 cut(s) | 263 |
| Bse1I | ACTGG | 4 cut(s) | 87, 352, 486, 509 |
| BseBI | CCWGG | 1 cut(s) | 244 |
| BseDI | CCNNGG | 2 cut(s) | 407, 420 |
| BseGI | GGATG | 1 cut(s) | 518 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 76, 151, 263, 445 |
| BseMII | CTCAG | 2 cut(s) | 129, 176 |
| BseNI | ACTGG | 4 cut(s) | 87, 352, 486, 509 |
| BseSI | GKGCMC | 1 cut(s) | 351 |
| BseYI | CCCAGC | 1 cut(s) | 472 |
| BshFI | GGCC | 4 cut(s) | 143, 158, 337, 471 |
| BsiHKAI | GWGCWC | 1 cut(s) | 242 |
| BsiHKCI | CYCGRG | 1 cut(s) | 400 |
| BsiSI | CCGG | 4 cut(s) | 71, 215, 264, 468 |
| BslFI | GGGAC | 1 cut(s) | 287 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 76, 151, 263, 445 |
| BsmAI | GTCTC | 1 cut(s) | 397 |
| BsmFI | GGGAC | 1 cut(s) | 287 |
| BsnI | GGCC | 4 cut(s) | 143, 158, 337, 471 |
| Bso31I | GGTCTC | 1 cut(s) | 397 |
| BsoBI | CYCGRG | 1 cut(s) | 400 |
| Bsp1286I | GDGCHC | 2 cut(s) | 242, 351 |
| Bsp143I | GATC | 3 cut(s) | 40, 88, 448 |
| BspACI | CCGC | 6 cut(s) | 15, 86, 305, 338, 397, 494 |
| BspANI | GGCC | 4 cut(s) | 143, 158, 337, 471 |
| BspCNI | CTCAG | 2 cut(s) | 130, 177 |
| BspLI | GGNNCC | 3 cut(s) | 303, 312, 336 |
| BspMI | ACCTGC | 1 cut(s) | 142 |
| BspPI | GGATC | 2 cut(s) | 96, 456 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspTNI | GGTCTC | 1 cut(s) | 397 |
| BsrBI | CCGCTC | 2 cut(s) | 399, 496 |
| BsrFI | RCCGGY | 1 cut(s) | 263 |
| BsrI | ACTGG | 4 cut(s) | 87, 352, 486, 509 |
| BssAI | RCCGGY | 1 cut(s) | 263 |
| BssECI | CCNNGG | 2 cut(s) | 407, 420 |
| BssMI | GATC | 3 cut(s) | 40, 88, 448 |
| BssT1I | CCWWGG | 1 cut(s) | 407 |
| Bst2UI | CCWGG | 1 cut(s) | 244 |
| Bst4CI | ACNGT | 1 cut(s) | 231 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 78 |
| BstDEI | CTNAG | 2 cut(s) | 138, 185 |
| BstF5I | GGATG | 1 cut(s) | 518 |
| BstH2I | RGCGCY | 1 cut(s) | 77 |
| BstHHI | GCGC | 3 cut(s) | 76, 126, 269 |
| BstKTI | GATC | 3 cut(s) | 43, 91, 451 |
| BstMAI | GTCTC | 1 cut(s) | 397 |
| BstMBI | GATC | 3 cut(s) | 40, 88, 448 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 215, 346, 482, 525 |
| BstNI | CCWGG | 1 cut(s) | 244 |
| BstSCI | CCNGG | 4 cut(s) | 69, 213, 242, 466 |
| BstSLI | GKGCMC | 1 cut(s) | 351 |
| BstX2I | RGATCY | 1 cut(s) | 88 |
| BstYI | RGATCY | 1 cut(s) | 88 |
| BsuRI | GGCC | 4 cut(s) | 143, 158, 337, 471 |
| BtgZI | GCGATG | 1 cut(s) | 500 |
| BtsCI | GGATG | 1 cut(s) | 518 |
| BtsI | GCAGTG | 1 cut(s) | 383 |
| BtsIMutI | CAGTG | 3 cut(s) | 80, 383, 479 |
| BveI | ACCTGC | 1 cut(s) | 142 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 78 |
| CfoI | GCGC | 3 cut(s) | 76, 126, 269 |
| Cfr10I | RCCGGY | 1 cut(s) | 263 |
| Cfr13I | GGNCC | 5 cut(s) | 142, 301, 310, 335, 470 |
| Csp6I | GTAC | 1 cut(s) | 261 |
| CviAII | CATG | 1 cut(s) | 219 |
| CviQI | GTAC | 1 cut(s) | 261 |
| DdeI | CTNAG | 2 cut(s) | 138, 185 |
| DpnI | GATC | 3 cut(s) | 42, 90, 450 |
| DpnII | GATC | 3 cut(s) | 40, 88, 448 |
| EaeI | YGGCCR | 1 cut(s) | 156 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 101 |
| Eco130I | CCWWGG | 1 cut(s) | 407 |
| Eco31I | GGTCTC | 1 cut(s) | 397 |
| Eco47I | GGWCC | 2 cut(s) | 301, 310 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 1 cut(s) | 400 |
| EcoO109I | RGGNCCY | 1 cut(s) | 301 |
| EcoRII | CCWGG | 1 cut(s) | 242 |
| EcoT14I | CCWWGG | 1 cut(s) | 407 |
| ErhI | CCWWGG | 1 cut(s) | 407 |
| FaeI | CATG | 1 cut(s) | 222 |
| FaiI | YATR | 4 cut(s) | 96, 220, 249, 294 |
| FaqI | GGGAC | 1 cut(s) | 287 |
| FatI | CATG | 1 cut(s) | 218 |
| FauI | CCCGC | 2 cut(s) | 8, 312 |
| Fnu4HI | GCNGC | 1 cut(s) | 338 |
| FokI | GGATG | 1 cut(s) | 525 |
| Fsp4HI | GCNGC | 1 cut(s) | 338 |
| FspBI | CTAG | 4 cut(s) | 258, 284, 317, 456 |
| GlaI | GCGC | 3 cut(s) | 75, 125, 268 |
| GluI | GCNGC | 1 cut(s) | 338 |
| GsaI | CCCAGC | 1 cut(s) | 476 |
| GsuI | CTGGAG | 1 cut(s) | 218 |
| HaeII | RGCGCY | 1 cut(s) | 77 |
| HaeIII | GGCC | 4 cut(s) | 143, 158, 337, 471 |
| HapII | CCGG | 4 cut(s) | 71, 215, 264, 468 |
| HhaI | GCGC | 3 cut(s) | 76, 126, 269 |
| Hin1II | CATG | 1 cut(s) | 222 |
| Hin6I | GCGC | 3 cut(s) | 74, 124, 267 |
| HinP1I | GCGC | 3 cut(s) | 74, 124, 267 |
| HinfI | GANTC | 1 cut(s) | 377 |
| HpaII | CCGG | 4 cut(s) | 71, 215, 264, 468 |
| Hpy166II | GTNNAC | 1 cut(s) | 116 |
| Hpy188III | TCNNGA | 1 cut(s) | 402 |
| Hpy8I | GTNNAC | 1 cut(s) | 116 |
| HpyCH4III | ACNGT | 1 cut(s) | 231 |
| HpyCH4IV | ACGT | 2 cut(s) | 112, 161 |
| HpyCH4V | TGCA | 3 cut(s) | 292, 388, 490 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 215, 346, 482, 525 |
| HpyF3I | CTNAG | 2 cut(s) | 138, 185 |
| HpySE526I | ACGT | 2 cut(s) | 112, 161 |
| Hsp92II | CATG | 1 cut(s) | 222 |
| HspAI | GCGC | 3 cut(s) | 74, 124, 267 |
| Kzo9I | GATC | 3 cut(s) | 40, 88, 448 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 3 cut(s) | 222, 237, 525 |
| LweI | GCATC | 1 cut(s) | 477 |
| MaeI | CTAG | 4 cut(s) | 258, 284, 317, 456 |
| MaeII | ACGT | 2 cut(s) | 112, 161 |
| MaeIII | GTNAC | 2 cut(s) | 168, 231 |
| MalI | GATC | 3 cut(s) | 42, 90, 450 |
| MbiI | CCGCTC | 2 cut(s) | 399, 496 |
| MboI | GATC | 3 cut(s) | 40, 88, 448 |
| MboII | GAAGA | 3 cut(s) | 41, 83, 201 |
| MflI | RGATCY | 1 cut(s) | 88 |
| MhlI | GDGCHC | 2 cut(s) | 242, 351 |
| MlsI | TGGCCA | 1 cut(s) | 158 |
| MluNI | TGGCCA | 1 cut(s) | 158 |
| MmeI | TCCRAC | 1 cut(s) | 503 |
| MnlI | CCTC | 4 cut(s) | 133, 193, 364, 415 |
| Mox20I | TGGCCA | 1 cut(s) | 158 |
| MscI | TGGCCA | 1 cut(s) | 158 |
| MseI | TTAA | 2 cut(s) | 356, 535 |
| MslI | CAYNNNNRTG | 2 cut(s) | 164, 177 |
| Msp20I | TGGCCA | 1 cut(s) | 158 |
| MspA1I | CMGCKG | 1 cut(s) | 307 |
| MspI | CCGG | 4 cut(s) | 71, 215, 264, 468 |
| MspR9I | CCNGG | 4 cut(s) | 71, 215, 244, 468 |
| MvaI | CCWGG | 1 cut(s) | 244 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 215, 346, 482, 525 |
| NciI | CCSGG | 3 cut(s) | 71, 215, 468 |
| NdeII | GATC | 3 cut(s) | 40, 88, 448 |
| NlaIII | CATG | 1 cut(s) | 222 |
| NlaIV | GGNNCC | 3 cut(s) | 303, 312, 336 |
| NmuCI | GTSAC | 2 cut(s) | 168, 231 |
| OliI | CACNNNNGTG | 1 cut(s) | 164 |
| PaeR7I | CTCGAG | 1 cut(s) | 400 |
| PaqCI | CACCTGC | 1 cut(s) | 142 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 377 |
| PkrI | GCNGC | 1 cut(s) | 339 |
| PpuMI | RGGWCCY | 1 cut(s) | 301 |
| Psp5II | RGGWCCY | 1 cut(s) | 301 |
| Psp6I | CCWGG | 1 cut(s) | 242 |
| PspFI | CCCAGC | 1 cut(s) | 472 |
| PspGI | CCWGG | 1 cut(s) | 242 |
| PspN4I | GGNNCC | 3 cut(s) | 303, 312, 336 |
| PspPI | GGNCC | 5 cut(s) | 142, 301, 310, 335, 470 |
| PspPPI | RGGWCCY | 1 cut(s) | 301 |
| PsuI | RGATCY | 1 cut(s) | 88 |
| RsaI | GTAC | 1 cut(s) | 262 |
| RsaNI | GTAC | 1 cut(s) | 261 |
| RseI | CAYNNNNRTG | 2 cut(s) | 164, 177 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 2 cut(s) | 356, 535 |
| SatI | GCNGC | 1 cut(s) | 338 |
| Sau3AI | GATC | 3 cut(s) | 40, 88, 448 |
| Sau96I | GGNCC | 5 cut(s) | 142, 301, 310, 335, 470 |
| ScrFI | CCNGG | 4 cut(s) | 71, 215, 244, 468 |
| SduI | GDGCHC | 2 cut(s) | 242, 351 |
| SetI | ASST | 7 cut(s) | 115, 156, 164, 259, 303, 324, 535 |
| SfaNI | GCATC | 1 cut(s) | 477 |
| Sfr274I | CTCGAG | 1 cut(s) | 400 |
| SinI | GGWCC | 2 cut(s) | 301, 310 |
| SlaI | CTCGAG | 1 cut(s) | 400 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 164, 177 |
| SmlI | CTYRAG | 1 cut(s) | 400 |
| SmoI | CTYRAG | 1 cut(s) | 400 |
| SpeI | ACTAGT | 1 cut(s) | 283 |
| SsiI | CCGC | 6 cut(s) | 15, 86, 305, 338, 397, 494 |
| SspMI | CTAG | 4 cut(s) | 258, 284, 317, 456 |
| StyD4I | CCNGG | 4 cut(s) | 69, 213, 242, 466 |
| StyI | CCWWGG | 1 cut(s) | 407 |
| TaaI | ACNGT | 1 cut(s) | 231 |
| TaiI | ACGT | 2 cut(s) | 115, 164 |
| TaqI | TCGA | 1 cut(s) | 401 |
| TauI | GCSGC | 1 cut(s) | 340 |
| TfiI | GAWTC | 1 cut(s) | 377 |
| Tru1I | TTAA | 2 cut(s) | 356, 535 |
| Tru9I | TTAA | 2 cut(s) | 356, 535 |
| TscAI | CASTG | 3 cut(s) | 87, 390, 486 |
| TseFI | GTSAC | 2 cut(s) | 168, 231 |
| Tsp45I | GTSAC | 2 cut(s) | 168, 231 |
| TspDTI | ATGAA | 2 cut(s) | 83, 195 |
| TspGWI | ACGGA | 1 cut(s) | 250 |
| TspRI | CASTG | 3 cut(s) | 87, 390, 486 |
| VpaK11BI | GGWCC | 2 cut(s) | 301, 310 |
| XhoI | CTCGAG | 1 cut(s) | 400 |
| XspI | CTAG | 4 cut(s) | 258, 284, 317, 456 |