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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 421 |
| AciI | CCGC | 4 cut(s) | 48, 92, 185, 254 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 231 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 5 cut(s) | 147, 159, 214, 219, 410 |
| Alw21I | GWGCWC | 1 cut(s) | 410 |
| AlwI | GGATC | 1 cut(s) | 231 |
| AoxI | GGCC | 5 cut(s) | 31, 189, 217, 309, 324 |
| ApeKI | GCWGC | 2 cut(s) | 182, 201 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AspLEI | GCGC | 3 cut(s) | 294, 364, 388 |
| AspS9I | GGNCC | 5 cut(s) | 31, 163, 190, 256, 310 |
| AsuC2I | CCSGG | 1 cut(s) | 198 |
| AsuHPI | GGTGA | 1 cut(s) | 200 |
| AvaII | GGWCC | 2 cut(s) | 163, 256 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanII | GRGCYC | 1 cut(s) | 155 |
| Bbv12I | GWGCWC | 1 cut(s) | 410 |
| BbvI | GCAGC | 2 cut(s) | 194, 213 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 5 cut(s) | 149, 161, 216, 221, 412 |
| BcnI | CCSGG | 1 cut(s) | 198 |
| BfaI | CTAG | 1 cut(s) | 135 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BisI | GCNGC | 2 cut(s) | 183, 202 |
| BlsI | GCNGC | 2 cut(s) | 184, 203 |
| Bme1390I | CCNGG | 6 cut(s) | 149, 161, 198, 216, 221, 412 |
| Bme18I | GGWCC | 2 cut(s) | 163, 256 |
| BmgT120I | GGNCC | 5 cut(s) | 31, 163, 190, 256, 310 |
| BmiI | GGNNCC | 2 cut(s) | 144, 165 |
| BmrFI | CCNGG | 6 cut(s) | 149, 161, 198, 216, 221, 412 |
| BpmI | CTGGAG | 1 cut(s) | 386 |
| BpuMI | CCSGG | 1 cut(s) | 198 |
| BsaJI | CCNNGG | 4 cut(s) | 27, 147, 148, 159 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| Bse118I | RCCGGY | 1 cut(s) | 431 |
| BseBI | CCWGG | 5 cut(s) | 149, 161, 216, 221, 412 |
| BseDI | CCNNGG | 4 cut(s) | 27, 147, 148, 159 |
| BseGI | GGATG | 2 cut(s) | 58, 155 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseMII | CTCAG | 1 cut(s) | 188 |
| BseXI | GCAGC | 2 cut(s) | 194, 213 |
| BseYI | CCCAGC | 1 cut(s) | 88 |
| Bsh1285I | CGRYCG | 1 cut(s) | 197 |
| BshFI | GGCC | 5 cut(s) | 33, 191, 219, 311, 326 |
| BsiEI | CGRYCG | 1 cut(s) | 197 |
| BsiHKAI | GWGCWC | 1 cut(s) | 410 |
| BsiSI | CCGG | 2 cut(s) | 197, 432 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 5 cut(s) | 33, 191, 219, 311, 326 |
| Bsp1286I | GDGCHC | 2 cut(s) | 155, 410 |
| Bsp143I | GATC | 2 cut(s) | 40, 223 |
| BspACI | CCGC | 4 cut(s) | 48, 92, 185, 254 |
| BspANI | GGCC | 5 cut(s) | 33, 191, 219, 311, 326 |
| BspCNI | CTCAG | 1 cut(s) | 187 |
| BspLI | GGNNCC | 2 cut(s) | 144, 165 |
| BspPI | GGATC | 1 cut(s) | 231 |
| BsrFI | RCCGGY | 1 cut(s) | 431 |
| BssAI | RCCGGY | 1 cut(s) | 431 |
| BssECI | CCNNGG | 4 cut(s) | 27, 147, 148, 159 |
| BssMI | GATC | 2 cut(s) | 40, 223 |
| Bst2UI | CCWGG | 5 cut(s) | 149, 161, 216, 221, 412 |
| BstC8I | GCNNGC | 2 cut(s) | 284, 395 |
| BstDEI | CTNAG | 1 cut(s) | 174 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 2 cut(s) | 58, 155 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 3 cut(s) | 294, 364, 388 |
| BstKTI | GATC | 2 cut(s) | 43, 226 |
| BstMBI | GATC | 2 cut(s) | 40, 223 |
| BstMCI | CGRYCG | 1 cut(s) | 197 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 98, 107, 188, 368, 383, 394 |
| BstNI | CCWGG | 5 cut(s) | 149, 161, 216, 221, 412 |
| BstSCI | CCNGG | 6 cut(s) | 147, 159, 196, 214, 219, 410 |
| BstV1I | GCAGC | 2 cut(s) | 194, 213 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| BsuRI | GGCC | 5 cut(s) | 33, 191, 219, 311, 326 |
| BtsCI | GGATG | 2 cut(s) | 58, 155 |
| Cac8I | GCNNGC | 2 cut(s) | 284, 395 |
| CfoI | GCGC | 3 cut(s) | 294, 364, 388 |
| Cfr10I | RCCGGY | 1 cut(s) | 431 |
| Cfr13I | GGNCC | 5 cut(s) | 31, 163, 190, 256, 310 |
| CviAII | CATG | 2 cut(s) | 71, 328 |
| CviJI | RGCY | 7 cut(s) | 33, 153, 191, 219, 311, 326, 435 |
| CviKI_1 | RGCY | 7 cut(s) | 33, 153, 191, 219, 311, 326, 435 |
| DdeI | CTNAG | 1 cut(s) | 174 |
| DpnI | GATC | 2 cut(s) | 42, 225 |
| DpnII | GATC | 2 cut(s) | 40, 223 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| EciI | GGCGGA | 2 cut(s) | 37, 269 |
| Eco24I | GRGCYC | 1 cut(s) | 155 |
| Eco47I | GGWCC | 2 cut(s) | 163, 256 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 163 |
| EcoRII | CCWGG | 5 cut(s) | 147, 159, 214, 219, 410 |
| EcoT38I | GRGCYC | 1 cut(s) | 155 |
| FaeI | CATG | 2 cut(s) | 74, 331 |
| FaiI | YATR | 4 cut(s) | 72, 236, 240, 329 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| FatI | CATG | 2 cut(s) | 70, 327 |
| FblI | GTMKAC | 1 cut(s) | 421 |
| Fnu4HI | GCNGC | 2 cut(s) | 183, 202 |
| FokI | GGATG | 2 cut(s) | 45, 142 |
| FriOI | GRGCYC | 1 cut(s) | 155 |
| Fsp4HI | GCNGC | 2 cut(s) | 183, 202 |
| FspBI | CTAG | 1 cut(s) | 135 |
| GlaI | GCGC | 3 cut(s) | 293, 363, 387 |
| GluI | GCNGC | 2 cut(s) | 183, 202 |
| GsaI | CCCAGC | 1 cut(s) | 92 |
| GsuI | CTGGAG | 1 cut(s) | 386 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 5 cut(s) | 33, 191, 219, 311, 326 |
| HapII | CCGG | 2 cut(s) | 197, 432 |
| HhaI | GCGC | 3 cut(s) | 294, 364, 388 |
| Hin1II | CATG | 2 cut(s) | 74, 331 |
| Hin6I | GCGC | 3 cut(s) | 292, 362, 386 |
| HinP1I | GCGC | 3 cut(s) | 292, 362, 386 |
| HincII | GTYRAC | 3 cut(s) | 85, 97, 171 |
| HindII | GTYRAC | 3 cut(s) | 85, 97, 171 |
| HinfI | GANTC | 2 cut(s) | 172, 423 |
| HpaI | GTTAAC | 2 cut(s) | 85, 97 |
| HpaII | CCGG | 2 cut(s) | 197, 432 |
| HphI | GGTGA | 1 cut(s) | 200 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 97, 171, 422 |
| Hpy188I | TCNGA | 1 cut(s) | 177 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 97, 171, 422 |
| Hpy99I | CGWCG | 1 cut(s) | 350 |
| HpyAV | CCTTC | 1 cut(s) | 352 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 1 cut(s) | 182 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 98, 107, 188, 368, 383, 394 |
| HpyF3I | CTNAG | 1 cut(s) | 174 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| Hsp92II | CATG | 2 cut(s) | 74, 331 |
| HspAI | GCGC | 3 cut(s) | 292, 362, 386 |
| KspAI | GTTAAC | 2 cut(s) | 85, 97 |
| Kzo9I | GATC | 2 cut(s) | 40, 223 |
| Lsp1109I | GCAGC | 2 cut(s) | 194, 213 |
| MaeI | CTAG | 1 cut(s) | 135 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MaeIII | GTNAC | 1 cut(s) | 206 |
| MalI | GATC | 2 cut(s) | 42, 225 |
| MboI | GATC | 2 cut(s) | 40, 223 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 2 cut(s) | 155, 410 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 1 cut(s) | 341 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MlyI | GAGTC | 2 cut(s) | 166, 417 |
| MnlI | CCTC | 5 cut(s) | 22, 181, 241, 301, 361 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 2 cut(s) | 84, 96 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 2 cut(s) | 92, 185 |
| MspI | CCGG | 2 cut(s) | 197, 432 |
| MspR9I | CCNGG | 6 cut(s) | 149, 161, 198, 216, 221, 412 |
| MvaI | CCWGG | 5 cut(s) | 149, 161, 216, 221, 412 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 98, 107, 188, 368, 383, 394 |
| NciI | CCSGG | 1 cut(s) | 198 |
| NdeII | GATC | 2 cut(s) | 40, 223 |
| NlaIII | CATG | 2 cut(s) | 74, 331 |
| NlaIV | GGNNCC | 2 cut(s) | 144, 165 |
| NmeAIII | GCCGAG | 1 cut(s) | 52 |
| NmuCI | GTSAC | 1 cut(s) | 206 |
| PasI | CCCWGGG | 1 cut(s) | 148 |
| PkrI | GCNGC | 2 cut(s) | 184, 203 |
| PleI | GAGTC | 2 cut(s) | 166, 417 |
| PpsI | GAGTC | 2 cut(s) | 166, 417 |
| PpuMI | RGGWCCY | 1 cut(s) | 163 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 163 |
| Psp6I | CCWGG | 5 cut(s) | 147, 159, 214, 219, 410 |
| PspFI | CCCAGC | 1 cut(s) | 88 |
| PspGI | CCWGG | 5 cut(s) | 147, 159, 214, 219, 410 |
| PspN4I | GGNNCC | 2 cut(s) | 144, 165 |
| PspPI | GGNCC | 5 cut(s) | 31, 163, 190, 256, 310 |
| PspPPI | RGGWCCY | 1 cut(s) | 163 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SaqAI | TTAA | 2 cut(s) | 84, 96 |
| SatI | GCNGC | 2 cut(s) | 183, 202 |
| Sau3AI | GATC | 2 cut(s) | 40, 223 |
| Sau96I | GGNCC | 5 cut(s) | 31, 163, 190, 256, 310 |
| SchI | GAGTC | 2 cut(s) | 166, 417 |
| ScrFI | CCNGG | 6 cut(s) | 149, 161, 198, 216, 221, 412 |
| SduI | GDGCHC | 2 cut(s) | 155, 410 |
| SetI | ASST | 4 cut(s) | 84, 165, 261, 306 |
| SinI | GGWCC | 2 cut(s) | 163, 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| Sse9I | AATT | 1 cut(s) | 341 |
| SsiI | CCGC | 4 cut(s) | 48, 92, 185, 254 |
| SspMI | CTAG | 1 cut(s) | 135 |
| StyD4I | CCNGG | 6 cut(s) | 147, 159, 196, 214, 219, 410 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TaqI | TCGA | 2 cut(s) | 306, 345 |
| TasI | AATT | 1 cut(s) | 341 |
| Tru1I | TTAA | 2 cut(s) | 84, 96 |
| Tru9I | TTAA | 2 cut(s) | 84, 96 |
| TseFI | GTSAC | 1 cut(s) | 206 |
| TseI | GCWGC | 2 cut(s) | 182, 201 |
| Tsp45I | GTSAC | 1 cut(s) | 206 |
| VpaK11BI | GGWCC | 2 cut(s) | 163, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 1 cut(s) | 341 |
| XmiI | GTMKAC | 1 cut(s) | 421 |
| XspI | CTAG | 1 cut(s) | 135 |