No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 382 |
| Acc65I | GGTACC | 1 cut(s) | 367 |
| AccB1I | GGYRCC | 1 cut(s) | 367 |
| AccI | GTMKAC | 1 cut(s) | 346 |
| AccII | CGCG | 4 cut(s) | 74, 147, 207, 328 |
| AciI | CCGC | 8 cut(s) | 38, 63, 72, 92, 179, 328, 360, 467 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 2 cut(s) | 48, 189 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 2 cut(s) | 369, 396 |
| AfeI | AGCGCT | 1 cut(s) | 288 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 356 |
| AgsI | TTSAA | 2 cut(s) | 118, 271 |
| AhlI | ACTAGT | 1 cut(s) | 376 |
| AluBI | AGCT | 2 cut(s) | 169, 311 |
| AluI | AGCT | 2 cut(s) | 169, 311 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 335 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 2 cut(s) | 48, 189 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 397 |
| Aor51HI | AGCGCT | 1 cut(s) | 288 |
| AoxI | GGCC | 1 cut(s) | 427 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApeKI | GCWGC | 1 cut(s) | 155 |
| Asp718I | GGTACC | 1 cut(s) | 367 |
| AspLEI | GCGC | 2 cut(s) | 207, 289 |
| AspS9I | GGNCC | 1 cut(s) | 428 |
| AsuC2I | CCSGG | 1 cut(s) | 89 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BanI | GGYRCC | 1 cut(s) | 367 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 335 |
| BbvI | GCAGC | 1 cut(s) | 142 |
| BccI | CCATC | 1 cut(s) | 66 |
| BceAI | ACGGC | 1 cut(s) | 90 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 137, 171 |
| BcnI | CCSGG | 1 cut(s) | 89 |
| BcuI | ACTAGT | 1 cut(s) | 376 |
| BfaI | CTAG | 2 cut(s) | 290, 377 |
| BfoI | RGCGCY | 1 cut(s) | 290 |
| BfuAI | ACCTGC | 1 cut(s) | 382 |
| BisI | GCNGC | 1 cut(s) | 156 |
| BlsI | GCNGC | 1 cut(s) | 157 |
| Bme1390I | CCNGG | 1 cut(s) | 89 |
| BmgT120I | GGNCC | 1 cut(s) | 428 |
| BmiI | GGNNCC | 1 cut(s) | 369 |
| BmrFI | CCNGG | 1 cut(s) | 89 |
| BmsI | GCATC | 1 cut(s) | 378 |
| BpuMI | CCSGG | 1 cut(s) | 89 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 455 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 356 |
| Bse118I | RCCGGY | 1 cut(s) | 356 |
| BseGI | GGATG | 2 cut(s) | 58, 439 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 356 |
| BseMII | CTCAG | 1 cut(s) | 326 |
| BseRI | GAGGAG | 1 cut(s) | 426 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseXI | GCAGC | 1 cut(s) | 142 |
| BseYI | CCCAGC | 1 cut(s) | 307 |
| BsgI | GTGCAG | 1 cut(s) | 170 |
| Bsh1236I | CGCG | 4 cut(s) | 74, 147, 207, 328 |
| BshFI | GGCC | 1 cut(s) | 429 |
| BshNI | GGYRCC | 1 cut(s) | 367 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 335 |
| BsiSI | CCGG | 2 cut(s) | 89, 357 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 356 |
| BsnI | GGCC | 1 cut(s) | 429 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 335 |
| Bsp143I | GATC | 2 cut(s) | 40, 181 |
| BspACI | CCGC | 8 cut(s) | 38, 63, 72, 92, 179, 328, 360, 467 |
| BspANI | GGCC | 1 cut(s) | 429 |
| BspCNI | CTCAG | 1 cut(s) | 325 |
| BspFNI | CGCG | 4 cut(s) | 74, 147, 207, 328 |
| BspLI | GGNNCC | 1 cut(s) | 369 |
| BspMI | ACCTGC | 1 cut(s) | 382 |
| BspPI | GGATC | 2 cut(s) | 48, 189 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 367 |
| BsrFI | RCCGGY | 1 cut(s) | 356 |
| BssAI | RCCGGY | 1 cut(s) | 356 |
| BssMI | GATC | 2 cut(s) | 40, 181 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 397 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 171, 209, 320 |
| BstDEI | CTNAG | 1 cut(s) | 312 |
| BstF5I | GGATG | 2 cut(s) | 58, 439 |
| BstFNI | CGCG | 4 cut(s) | 74, 147, 207, 328 |
| BstH2I | RGCGCY | 1 cut(s) | 290 |
| BstHHI | GCGC | 2 cut(s) | 207, 289 |
| BstKTI | GATC | 2 cut(s) | 43, 184 |
| BstMBI | GATC | 2 cut(s) | 40, 181 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 71, 293, 397 |
| BstSCI | CCNGG | 1 cut(s) | 87 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 4 cut(s) | 74, 147, 207, 328 |
| BstV1I | GCAGC | 1 cut(s) | 142 |
| BstX2I | RGATCY | 1 cut(s) | 181 |
| BstYI | RGATCY | 1 cut(s) | 181 |
| BsuRI | GGCC | 1 cut(s) | 429 |
| BtsCI | GGATG | 2 cut(s) | 58, 439 |
| BveI | ACCTGC | 1 cut(s) | 382 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 171, 209, 320 |
| CaiI | CAGNNNCTG | 1 cut(s) | 397 |
| CfoI | GCGC | 2 cut(s) | 207, 289 |
| Cfr10I | RCCGGY | 1 cut(s) | 356 |
| Cfr13I | GGNCC | 1 cut(s) | 428 |
| Csp6I | GTAC | 2 cut(s) | 368, 395 |
| CviJI | RGCY | 5 cut(s) | 105, 169, 236, 311, 429 |
| CviKI_1 | RGCY | 5 cut(s) | 105, 169, 236, 311, 429 |
| CviQI | GTAC | 2 cut(s) | 368, 395 |
| DdeI | CTNAG | 1 cut(s) | 312 |
| DpnI | GATC | 2 cut(s) | 42, 183 |
| DpnII | GATC | 2 cut(s) | 40, 181 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 2 cut(s) | 194, 375 |
| Eco47III | AGCGCT | 1 cut(s) | 288 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| FaiI | YATR | 4 cut(s) | 161, 165, 383, 475 |
| FauI | CCCGC | 2 cut(s) | 70, 79 |
| FblI | GTMKAC | 1 cut(s) | 346 |
| Fnu4HI | GCNGC | 1 cut(s) | 156 |
| FokI | GGATG | 2 cut(s) | 45, 426 |
| Fsp4HI | GCNGC | 1 cut(s) | 156 |
| FspBI | CTAG | 2 cut(s) | 290, 377 |
| GlaI | GCGC | 2 cut(s) | 206, 288 |
| GluI | GCNGC | 1 cut(s) | 156 |
| GsaI | CCCAGC | 1 cut(s) | 311 |
| HaeII | RGCGCY | 1 cut(s) | 290 |
| HaeIII | GGCC | 1 cut(s) | 429 |
| HapII | CCGG | 2 cut(s) | 89, 357 |
| HhaI | GCGC | 2 cut(s) | 207, 289 |
| Hin6I | GCGC | 2 cut(s) | 205, 287 |
| HinP1I | GCGC | 2 cut(s) | 205, 287 |
| HincII | GTYRAC | 4 cut(s) | 97, 130, 304, 347 |
| HindII | GTYRAC | 4 cut(s) | 97, 130, 304, 347 |
| HinfI | GANTC | 1 cut(s) | 470 |
| HpaI | GTTAAC | 2 cut(s) | 97, 304 |
| HpaII | CCGG | 2 cut(s) | 89, 357 |
| Hpy166II | GTNNAC | 5 cut(s) | 97, 110, 130, 304, 347 |
| Hpy188I | TCNGA | 2 cut(s) | 141, 315 |
| Hpy8I | GTNNAC | 5 cut(s) | 97, 110, 130, 304, 347 |
| HpyAV | CCTTC | 1 cut(s) | 220 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 2 cut(s) | 99, 340 |
| HpyCH4V | TGCA | 3 cut(s) | 110, 187, 391 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 71, 293, 397 |
| HpyF3I | CTNAG | 1 cut(s) | 312 |
| HpySE526I | ACGT | 2 cut(s) | 99, 340 |
| HspAI | GCGC | 2 cut(s) | 205, 287 |
| KpnI | GGTACC | 1 cut(s) | 371 |
| KspAI | GTTAAC | 2 cut(s) | 97, 304 |
| Kzo9I | GATC | 2 cut(s) | 40, 181 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 330, 457 |
| Lsp1109I | GCAGC | 1 cut(s) | 142 |
| LweI | GCATC | 1 cut(s) | 378 |
| MaeI | CTAG | 2 cut(s) | 290, 377 |
| MaeII | ACGT | 2 cut(s) | 99, 340 |
| MaeIII | GTNAC | 1 cut(s) | 250 |
| MalI | GATC | 2 cut(s) | 42, 183 |
| MboI | GATC | 2 cut(s) | 40, 181 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MflI | RGATCY | 1 cut(s) | 181 |
| MhlI | GDGCHC | 2 cut(s) | 112, 335 |
| MluCI | AATT | 1 cut(s) | 275 |
| MmeI | TCCRAC | 1 cut(s) | 435 |
| MnlI | CCTC | 7 cut(s) | 161, 226, 286, 444, 447, 455, 457 |
| MseI | TTAA | 5 cut(s) | 84, 96, 278, 303, 449 |
| MslI | CAYNNNNRTG | 1 cut(s) | 386 |
| MspI | CCGG | 2 cut(s) | 89, 357 |
| MspR9I | CCNGG | 1 cut(s) | 89 |
| MvnI | CGCG | 4 cut(s) | 74, 147, 207, 328 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 71, 293, 397 |
| NciI | CCSGG | 1 cut(s) | 89 |
| NdeII | GATC | 2 cut(s) | 40, 181 |
| NlaIV | GGNNCC | 1 cut(s) | 369 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PflFI | GACNNNGTC | 1 cut(s) | 342 |
| PkrI | GCNGC | 1 cut(s) | 157 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| PspFI | CCCAGC | 1 cut(s) | 307 |
| PspN4I | GGNNCC | 1 cut(s) | 369 |
| PspPI | GGNCC | 1 cut(s) | 428 |
| PstNI | CAGNNNCTG | 1 cut(s) | 397 |
| PsuI | RGATCY | 1 cut(s) | 181 |
| PsyI | GACNNNGTC | 1 cut(s) | 342 |
| RsaI | GTAC | 2 cut(s) | 369, 396 |
| RsaNI | GTAC | 2 cut(s) | 368, 395 |
| RseI | CAYNNNNRTG | 1 cut(s) | 386 |
| SalI | GTCGAC | 1 cut(s) | 345 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 5 cut(s) | 84, 96, 278, 303, 449 |
| SatI | GCNGC | 1 cut(s) | 156 |
| Sau3AI | GATC | 2 cut(s) | 40, 181 |
| Sau96I | GGNCC | 1 cut(s) | 428 |
| ScrFI | CCNGG | 1 cut(s) | 89 |
| SduI | GDGCHC | 2 cut(s) | 112, 335 |
| SetI | ASST | 7 cut(s) | 102, 153, 171, 313, 343, 396, 436 |
| SfaNI | GCATC | 1 cut(s) | 378 |
| SgrAI | CRCCGGYG | 1 cut(s) | 356 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 386 |
| SpeI | ACTAGT | 1 cut(s) | 376 |
| Sse9I | AATT | 1 cut(s) | 275 |
| SsiI | CCGC | 8 cut(s) | 38, 63, 72, 92, 179, 328, 360, 467 |
| SspMI | CTAG | 2 cut(s) | 290, 377 |
| StyD4I | CCNGG | 1 cut(s) | 87 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 2 cut(s) | 102, 343 |
| TaqI | TCGA | 1 cut(s) | 346 |
| TasI | AATT | 1 cut(s) | 275 |
| Tru1I | TTAA | 5 cut(s) | 84, 96, 278, 303, 449 |
| Tru9I | TTAA | 5 cut(s) | 84, 96, 278, 303, 449 |
| TseI | GCWGC | 1 cut(s) | 155 |
| Tth111I | GACNNNGTC | 1 cut(s) | 342 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| XmiI | GTMKAC | 1 cut(s) | 346 |
| XspI | CTAG | 2 cut(s) | 290, 377 |