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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 202 |
| AciI | CCGC | 3 cut(s) | 88, 200, 216 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 121 |
| AgsI | TTSAA | 1 cut(s) | 100 |
| AjnI | CCWGG | 1 cut(s) | 115 |
| AluBI | AGCT | 2 cut(s) | 229, 296 |
| AluI | AGCT | 2 cut(s) | 229, 296 |
| Alw26I | GTCTC | 1 cut(s) | 152 |
| AoxI | GGCC | 1 cut(s) | 288 |
| AspLEI | GCGC | 1 cut(s) | 204 |
| AspS9I | GGNCC | 2 cut(s) | 145, 241 |
| AsuHPI | GGTGA | 1 cut(s) | 167 |
| AvaII | GGWCC | 2 cut(s) | 145, 241 |
| AxyI | CCTNAGG | 1 cut(s) | 141 |
| BanII | GRGCYC | 1 cut(s) | 129 |
| BceAI | ACGGC | 1 cut(s) | 90 |
| BciT130I | CCWGG | 1 cut(s) | 117 |
| BcoDI | GTCTC | 1 cut(s) | 152 |
| BfmI | CTRYAG | 1 cut(s) | 162 |
| BisI | GCNGC | 1 cut(s) | 216 |
| BlsI | GCNGC | 1 cut(s) | 217 |
| Bme1390I | CCNGG | 1 cut(s) | 117 |
| Bme18I | GGWCC | 2 cut(s) | 145, 241 |
| BmgT120I | GGNCC | 2 cut(s) | 145, 241 |
| BmiI | GGNNCC | 2 cut(s) | 126, 147 |
| BmrFI | CCNGG | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 115, 178 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 121 |
| Bse1I | ACTGG | 2 cut(s) | 43, 240 |
| Bse21I | CCTNAGG | 1 cut(s) | 141 |
| BseBI | CCWGG | 1 cut(s) | 117 |
| BseDI | CCNNGG | 3 cut(s) | 72, 115, 178 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BseMII | CTCAG | 2 cut(s) | 147, 155 |
| BseNI | ACTGG | 2 cut(s) | 43, 240 |
| BseRI | GAGGAG | 1 cut(s) | 195 |
| Bsh1236I | CGCG | 1 cut(s) | 202 |
| BshFI | GGCC | 1 cut(s) | 290 |
| BslFI | GGGAC | 1 cut(s) | 131 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BsmAI | GTCTC | 1 cut(s) | 152 |
| BsmFI | GGGAC | 1 cut(s) | 131 |
| BsmI | GAATGC | 1 cut(s) | 281 |
| BsnI | GGCC | 1 cut(s) | 290 |
| Bsp1286I | GDGCHC | 1 cut(s) | 129 |
| BspACI | CCGC | 3 cut(s) | 88, 200, 216 |
| BspANI | GGCC | 1 cut(s) | 290 |
| BspCNI | CTCAG | 2 cut(s) | 148, 154 |
| BspFNI | CGCG | 1 cut(s) | 202 |
| BspLI | GGNNCC | 2 cut(s) | 126, 147 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrI | ACTGG | 2 cut(s) | 43, 240 |
| BssECI | CCNNGG | 3 cut(s) | 72, 115, 178 |
| Bst2UI | CCWGG | 1 cut(s) | 117 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 166 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 101 |
| BstC8I | GCNNGC | 1 cut(s) | 269 |
| BstDEI | CTNAG | 2 cut(s) | 141, 156 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstFNI | CGCG | 1 cut(s) | 202 |
| BstHHI | GCGC | 1 cut(s) | 204 |
| BstMAI | GTCTC | 1 cut(s) | 152 |
| BstNI | CCWGG | 1 cut(s) | 117 |
| BstSCI | CCNGG | 1 cut(s) | 115 |
| BstSFI | CTRYAG | 1 cut(s) | 162 |
| BstUI | CGCG | 1 cut(s) | 202 |
| Bsu36I | CCTNAGG | 1 cut(s) | 141 |
| BsuRI | GGCC | 1 cut(s) | 290 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsIMutI | CAGTG | 1 cut(s) | 171 |
| Cac8I | GCNNGC | 1 cut(s) | 269 |
| CfoI | GCGC | 1 cut(s) | 204 |
| Cfr13I | GGNCC | 2 cut(s) | 145, 241 |
| CviJI | RGCY | 6 cut(s) | 77, 127, 173, 229, 290, 296 |
| CviKI_1 | RGCY | 6 cut(s) | 77, 127, 173, 229, 290, 296 |
| DdeI | CTNAG | 2 cut(s) | 141, 156 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 101 |
| EarI | CTCTTC | 2 cut(s) | 24, 101 |
| Eco24I | GRGCYC | 1 cut(s) | 129 |
| Eco47I | GGWCC | 2 cut(s) | 145, 241 |
| Eco81I | CCTNAGG | 1 cut(s) | 141 |
| EcoO109I | RGGNCCY | 1 cut(s) | 145 |
| EcoRII | CCWGG | 1 cut(s) | 115 |
| EcoT38I | GRGCYC | 1 cut(s) | 129 |
| FaiI | YATR | 1 cut(s) | 225 |
| FaqI | GGGAC | 1 cut(s) | 131 |
| Fnu4HI | GCNGC | 1 cut(s) | 216 |
| FriOI | GRGCYC | 1 cut(s) | 129 |
| Fsp4HI | GCNGC | 1 cut(s) | 216 |
| GlaI | GCGC | 1 cut(s) | 203 |
| GluI | GCNGC | 1 cut(s) | 216 |
| HaeIII | GGCC | 1 cut(s) | 290 |
| HhaI | GCGC | 1 cut(s) | 204 |
| Hin6I | GCGC | 1 cut(s) | 202 |
| HinP1I | GCGC | 1 cut(s) | 202 |
| HincII | GTYRAC | 3 cut(s) | 52, 85, 112 |
| HindII | GTYRAC | 3 cut(s) | 52, 85, 112 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HphI | GGTGA | 1 cut(s) | 167 |
| Hpy166II | GTNNAC | 4 cut(s) | 52, 85, 112, 241 |
| Hpy8I | GTNNAC | 4 cut(s) | 52, 85, 112, 241 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 166 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 2 cut(s) | 267, 279 |
| HpyF3I | CTNAG | 2 cut(s) | 141, 156 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| HspAI | GCGC | 1 cut(s) | 202 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 124 |
| LpnPI | CCDG | 8 cut(s) | 56, 102, 120, 128, 129, 215, 221, 244 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MboII | GAAGA | 2 cut(s) | 41, 88 |
| MhlI | GDGCHC | 1 cut(s) | 129 |
| MnlI | CCTC | 5 cut(s) | 150, 163, 173, 176, 254 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspR9I | CCNGG | 1 cut(s) | 117 |
| Mva1269I | GAATGC | 1 cut(s) | 281 |
| MvaI | CCWGG | 1 cut(s) | 117 |
| MvnI | CGCG | 1 cut(s) | 202 |
| NlaIV | GGNNCC | 2 cut(s) | 126, 147 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 281 |
| PkrI | GCNGC | 1 cut(s) | 217 |
| PpuMI | RGGWCCY | 1 cut(s) | 145 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 145 |
| Psp6I | CCWGG | 1 cut(s) | 115 |
| PspGI | CCWGG | 1 cut(s) | 115 |
| PspN4I | GGNNCC | 2 cut(s) | 126, 147 |
| PspPI | GGNCC | 2 cut(s) | 145, 241 |
| PspPPI | RGGWCCY | 1 cut(s) | 145 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 145, 177 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 1 cut(s) | 216 |
| Sau96I | GGNCC | 2 cut(s) | 145, 241 |
| ScrFI | CCNGG | 1 cut(s) | 117 |
| SduI | GDGCHC | 1 cut(s) | 129 |
| SetI | ASST | 7 cut(s) | 68, 84, 147, 213, 231, 246, 298 |
| SfcI | CTRYAG | 1 cut(s) | 162 |
| SinI | GGWCC | 2 cut(s) | 145, 241 |
| SsiI | CCGC | 3 cut(s) | 88, 200, 216 |
| StyD4I | CCNGG | 1 cut(s) | 115 |
| TaaI | ACNGT | 2 cut(s) | 11, 166 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TauI | GCSGC | 1 cut(s) | 218 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 171 |
| TspGWI | ACGGA | 2 cut(s) | 37, 137 |
| TspRI | CASTG | 1 cut(s) | 171 |
| VpaK11BI | GGWCC | 2 cut(s) | 145, 241 |