No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 310 |
| Acc36I | ACCTGC | 1 cut(s) | 310 |
| AccBSI | CCGCTC | 1 cut(s) | 201 |
| AccII | CGCG | 1 cut(s) | 403 |
| AciI | CCGC | 5 cut(s) | 138, 201, 208, 254, 403 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 1 cut(s) | 157 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcuI | CTGAAG | 1 cut(s) | 374 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 214, 265 |
| AjnI | CCWGG | 2 cut(s) | 147, 410 |
| AluBI | AGCT | 2 cut(s) | 244, 352 |
| AluI | AGCT | 2 cut(s) | 244, 352 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 410 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 1 cut(s) | 157 |
| AoxI | GGCC | 2 cut(s) | 150, 324 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| AspLEI | GCGC | 3 cut(s) | 294, 388, 417 |
| AspS9I | GGNCC | 2 cut(s) | 223, 256 |
| AvaII | GGWCC | 2 cut(s) | 223, 256 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 410 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 2 cut(s) | 149, 412 |
| BclI | TGATCA | 2 cut(s) | 40, 75 |
| BfaI | CTAG | 1 cut(s) | 251 |
| BfmI | CTRYAG | 1 cut(s) | 180 |
| BfuAI | ACCTGC | 1 cut(s) | 310 |
| BisI | GCNGC | 1 cut(s) | 208 |
| BlpI | GCTNAGC | 1 cut(s) | 192 |
| BlsI | GCNGC | 1 cut(s) | 209 |
| Bme1390I | CCNGG | 2 cut(s) | 149, 412 |
| Bme18I | GGWCC | 2 cut(s) | 223, 256 |
| BmgT120I | GGNCC | 2 cut(s) | 223, 256 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 2 cut(s) | 149, 412 |
| BpmI | CTGGAG | 1 cut(s) | 118 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 192 |
| BsaJI | CCNNGG | 1 cut(s) | 147 |
| BsaWI | WCCGGW | 1 cut(s) | 214 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 214, 265 |
| Bse118I | RCCGGY | 1 cut(s) | 124 |
| Bse1I | ACTGG | 1 cut(s) | 153 |
| BseBI | CCWGG | 2 cut(s) | 149, 412 |
| BseDI | CCNNGG | 1 cut(s) | 147 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 214, 265 |
| BseMII | CTCAG | 2 cut(s) | 18, 206 |
| BseNI | ACTGG | 1 cut(s) | 153 |
| BseRI | GAGGAG | 2 cut(s) | 44, 200 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseYI | CCCAGC | 1 cut(s) | 382 |
| BsgI | GTGCAG | 1 cut(s) | 239 |
| Bsh1236I | CGCG | 1 cut(s) | 403 |
| BshFI | GGCC | 2 cut(s) | 152, 326 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 410 |
| BsiSI | CCGG | 2 cut(s) | 125, 215 |
| BslFI | GGGAC | 1 cut(s) | 324 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 214, 265 |
| BsmFI | GGGAC | 1 cut(s) | 324 |
| BsnI | GGCC | 2 cut(s) | 152, 326 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 410 |
| Bsp143I | GATC | 3 cut(s) | 40, 75, 162 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 192 |
| BspACI | CCGC | 5 cut(s) | 138, 201, 208, 254, 403 |
| BspANI | GGCC | 2 cut(s) | 152, 326 |
| BspCNI | CTCAG | 2 cut(s) | 19, 205 |
| BspFNI | CGCG | 1 cut(s) | 403 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspMI | ACCTGC | 1 cut(s) | 310 |
| BspPI | GGATC | 1 cut(s) | 157 |
| BsrBI | CCGCTC | 1 cut(s) | 201 |
| BsrFI | RCCGGY | 1 cut(s) | 124 |
| BsrI | ACTGG | 1 cut(s) | 153 |
| BssAI | RCCGGY | 1 cut(s) | 124 |
| BssECI | CCNNGG | 1 cut(s) | 147 |
| BssMI | GATC | 3 cut(s) | 40, 75, 162 |
| Bst2UI | CCWGG | 2 cut(s) | 149, 412 |
| Bst4CI | ACNGT | 2 cut(s) | 94, 107 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 122, 246, 284 |
| BstDEI | CTNAG | 2 cut(s) | 27, 192 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 1 cut(s) | 403 |
| BstHHI | GCGC | 3 cut(s) | 294, 388, 417 |
| BstKTI | GATC | 3 cut(s) | 43, 78, 165 |
| BstMBI | GATC | 3 cut(s) | 40, 75, 162 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 207, 383, 394, 414 |
| BstNI | CCWGG | 2 cut(s) | 149, 412 |
| BstNSI | RCATGY | 1 cut(s) | 124 |
| BstSCI | CCNGG | 2 cut(s) | 147, 410 |
| BstSFI | CTRYAG | 1 cut(s) | 180 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 1 cut(s) | 403 |
| BstX2I | RGATCY | 1 cut(s) | 162 |
| BstYI | RGATCY | 1 cut(s) | 162 |
| BsuRI | GGCC | 2 cut(s) | 152, 326 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsI | GCAGTG | 1 cut(s) | 78 |
| BtsIMutI | CAGTG | 2 cut(s) | 24, 78 |
| BveI | ACCTGC | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 122, 246, 284 |
| CfoI | GCGC | 3 cut(s) | 294, 388, 417 |
| Cfr10I | RCCGGY | 1 cut(s) | 124 |
| Cfr13I | GGNCC | 2 cut(s) | 223, 256 |
| CviAII | CATG | 1 cut(s) | 121 |
| CviJI | RGCY | 8 cut(s) | 38, 152, 191, 196, 207, 244, 326, 352 |
| CviKI_1 | RGCY | 8 cut(s) | 38, 152, 191, 196, 207, 244, 326, 352 |
| DdeI | CTNAG | 2 cut(s) | 27, 192 |
| DpnI | GATC | 3 cut(s) | 42, 77, 164 |
| DpnII | GATC | 3 cut(s) | 40, 75, 162 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| Eco47I | GGWCC | 2 cut(s) | 223, 256 |
| Eco57I | CTGAAG | 1 cut(s) | 374 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| EcoRII | CCWGG | 2 cut(s) | 147, 410 |
| FaeI | CATG | 1 cut(s) | 124 |
| FaiI | YATR | 3 cut(s) | 122, 236, 240 |
| FaqI | GGGAC | 1 cut(s) | 324 |
| FatI | CATG | 1 cut(s) | 120 |
| FauI | CCCGC | 1 cut(s) | 131 |
| FbaI | TGATCA | 2 cut(s) | 40, 75 |
| Fnu4HI | GCNGC | 1 cut(s) | 208 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 1 cut(s) | 208 |
| FspBI | CTAG | 1 cut(s) | 251 |
| GlaI | GCGC | 3 cut(s) | 293, 387, 416 |
| GluI | GCNGC | 1 cut(s) | 208 |
| GsaI | CCCAGC | 1 cut(s) | 386 |
| GsuI | CTGGAG | 1 cut(s) | 118 |
| HaeIII | GGCC | 2 cut(s) | 152, 326 |
| HapII | CCGG | 2 cut(s) | 125, 215 |
| HhaI | GCGC | 3 cut(s) | 294, 388, 417 |
| Hin1II | CATG | 1 cut(s) | 124 |
| Hin6I | GCGC | 3 cut(s) | 292, 386, 415 |
| HinP1I | GCGC | 3 cut(s) | 292, 386, 415 |
| HincII | GTYRAC | 3 cut(s) | 97, 103, 130 |
| HindII | GTYRAC | 3 cut(s) | 97, 103, 130 |
| HpaI | GTTAAC | 2 cut(s) | 97, 103 |
| HpaII | CCGG | 2 cut(s) | 125, 215 |
| Hpy166II | GTNNAC | 4 cut(s) | 97, 103, 110, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 339 |
| Hpy8I | GTNNAC | 4 cut(s) | 97, 103, 110, 130 |
| HpyAV | CCTTC | 2 cut(s) | 269, 352 |
| HpyCH4III | ACNGT | 2 cut(s) | 94, 107 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 2 cut(s) | 110, 220 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 207, 383, 394, 414 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 192 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| Hsp92II | CATG | 1 cut(s) | 124 |
| HspAI | GCGC | 3 cut(s) | 292, 386, 415 |
| Ksp22I | TGATCA | 2 cut(s) | 40, 75 |
| KspAI | GTTAAC | 2 cut(s) | 97, 103 |
| Kzo9I | GATC | 3 cut(s) | 40, 75, 162 |
| LmnI | GCTCC | 4 cut(s) | 31, 289, 394, 405 |
| MaeI | CTAG | 1 cut(s) | 251 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MaeIII | GTNAC | 1 cut(s) | 155 |
| MalI | GATC | 3 cut(s) | 42, 77, 164 |
| MbiI | CCGCTC | 1 cut(s) | 201 |
| MboI | GATC | 3 cut(s) | 40, 75, 162 |
| MboII | GAAGA | 1 cut(s) | 367 |
| MflI | RGATCY | 1 cut(s) | 162 |
| MhlI | GDGCHC | 2 cut(s) | 112, 410 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MmeI | TCCRAC | 1 cut(s) | 113 |
| MnlI | CCTC | 6 cut(s) | 22, 178, 181, 222, 236, 301 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 2 cut(s) | 96, 102 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 138 |
| MspI | CCGG | 2 cut(s) | 125, 215 |
| MspR9I | CCNGG | 2 cut(s) | 149, 412 |
| MvaI | CCWGG | 2 cut(s) | 149, 412 |
| MvnI | CGCG | 1 cut(s) | 403 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 207, 383, 394, 414 |
| NdeII | GATC | 3 cut(s) | 40, 75, 162 |
| NlaIII | CATG | 1 cut(s) | 124 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NmeAIII | GCCGAG | 1 cut(s) | 222 |
| NspI | RCATGY | 1 cut(s) | 124 |
| PaeI | GCATGC | 1 cut(s) | 124 |
| PaqCI | CACCTGC | 1 cut(s) | 310 |
| PkrI | GCNGC | 1 cut(s) | 209 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| Psp6I | CCWGG | 2 cut(s) | 147, 410 |
| PspFI | CCCAGC | 1 cut(s) | 382 |
| PspGI | CCWGG | 2 cut(s) | 147, 410 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 2 cut(s) | 223, 256 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| PsuI | RGATCY | 1 cut(s) | 162 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SaqAI | TTAA | 2 cut(s) | 96, 102 |
| SatI | GCNGC | 1 cut(s) | 208 |
| Sau3AI | GATC | 3 cut(s) | 40, 75, 162 |
| Sau96I | GGNCC | 2 cut(s) | 223, 256 |
| ScrFI | CCNGG | 2 cut(s) | 149, 412 |
| SduI | GDGCHC | 2 cut(s) | 112, 410 |
| SetI | ASST | 9 cut(s) | 84, 102, 228, 246, 261, 306, 324, 354, 363 |
| SfcI | CTRYAG | 1 cut(s) | 180 |
| SinI | GGWCC | 2 cut(s) | 223, 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| SphI | GCATGC | 1 cut(s) | 124 |
| SsiI | CCGC | 5 cut(s) | 138, 201, 208, 254, 403 |
| SspMI | CTAG | 1 cut(s) | 251 |
| StyD4I | CCNGG | 2 cut(s) | 147, 410 |
| TaaI | ACNGT | 2 cut(s) | 94, 107 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 1 cut(s) | 306 |
| TauI | GCSGC | 1 cut(s) | 210 |
| Tru1I | TTAA | 2 cut(s) | 96, 102 |
| Tru9I | TTAA | 2 cut(s) | 96, 102 |
| TscAI | CASTG | 2 cut(s) | 31, 78 |
| TspRI | CASTG | 2 cut(s) | 31, 78 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 2 cut(s) | 223, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XceI | RCATGY | 1 cut(s) | 124 |
| XspI | CTAG | 1 cut(s) | 251 |