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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 394 |
| AccBSI | CCGCTC | 3 cut(s) | 542, 602, 650 |
| AccII | CGCG | 2 cut(s) | 90, 691 |
| AclWI | GGATC | 3 cut(s) | 201, 562, 781 |
| AcoI | YGGCCR | 2 cut(s) | 261, 728 |
| AdeI | CACNNNGTG | 2 cut(s) | 329, 854 |
| AfaI | GTAC | 2 cut(s) | 453, 571 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 551 |
| AgsI | TTSAA | 2 cut(s) | 677, 813 |
| AjnI | CCWGG | 7 cut(s) | 133, 151, 347, 545, 572, 694, 699 |
| AluBI | AGCT | 5 cut(s) | 181, 289, 427, 460, 673 |
| AluI | AGCT | 5 cut(s) | 181, 289, 427, 460, 673 |
| Alw26I | GTCTC | 2 cut(s) | 119, 502 |
| AlwI | GGATC | 3 cut(s) | 201, 562, 781 |
| Ama87I | CYCGRG | 2 cut(s) | 505, 513 |
| AoxI | GGCC | 6 cut(s) | 154, 240, 246, 261, 697, 728 |
| ApeKI | GCWGC | 2 cut(s) | 141, 412 |
| AspLEI | GCGC | 3 cut(s) | 92, 231, 347 |
| AspS9I | GGNCC | 4 cut(s) | 247, 406, 440, 703 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 320 |
| AsuHPI | GGTGA | 4 cut(s) | 91, 134, 602, 813 |
| AsuNHI | GCTAGC | 1 cut(s) | 427 |
| AvaI | CYCGRG | 2 cut(s) | 505, 513 |
| AvaII | GGWCC | 3 cut(s) | 406, 440, 703 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 255, 288 |
| BalI | TGGCCA | 2 cut(s) | 263, 730 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| BbvI | GCAGC | 2 cut(s) | 153, 399 |
| BccI | CCATC | 1 cut(s) | 739 |
| BciT130I | CCWGG | 7 cut(s) | 135, 153, 349, 547, 574, 696, 701 |
| BciVI | GTATCC | 1 cut(s) | 283 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BcoDI | GTCTC | 2 cut(s) | 119, 502 |
| BfaI | CTAG | 2 cut(s) | 428, 578 |
| BfoI | RGCGCY | 1 cut(s) | 348 |
| BfuAI | ACCTGC | 1 cut(s) | 394 |
| BfuI | GTATCC | 1 cut(s) | 283 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 320 |
| BisI | GCNGC | 3 cut(s) | 142, 145, 413 |
| BlsI | GCNGC | 3 cut(s) | 143, 146, 414 |
| Bme18I | GGWCC | 3 cut(s) | 406, 440, 703 |
| BmeT110I | CYCGRG | 2 cut(s) | 505, 513 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 406, 440, 703 |
| BmiI | GGNNCC | 3 cut(s) | 408, 441, 661 |
| BmsI | GCATC | 2 cut(s) | 583, 609 |
| BmtI | GCTAGC | 1 cut(s) | 431 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BsaI | GGTCTC | 1 cut(s) | 502 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 115, 133, 243, 512, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 551 |
| Bse118I | RCCGGY | 2 cut(s) | 587, 738 |
| Bse1I | ACTGG | 2 cut(s) | 389, 615 |
| Bse3DI | GCAATG | 1 cut(s) | 499 |
| BseBI | CCWGG | 7 cut(s) | 135, 153, 349, 547, 574, 696, 701 |
| BseDI | CCNNGG | 6 cut(s) | 105, 115, 133, 243, 512, 545 |
| BseGI | GGATG | 1 cut(s) | 624 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 551 |
| BseMI | GCAATG | 1 cut(s) | 499 |
| BseNI | ACTGG | 2 cut(s) | 389, 615 |
| BseXI | GCAGC | 2 cut(s) | 153, 399 |
| BseYI | CCCAGC | 3 cut(s) | 70, 456, 663 |
| BsgI | GTGCAG | 3 cut(s) | 349, 398, 476 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 691 |
| BshFI | GGCC | 6 cut(s) | 156, 242, 248, 263, 699, 730 |
| BsiHKCI | CYCGRG | 2 cut(s) | 505, 513 |
| BsiSI | CCGG | 5 cut(s) | 107, 117, 320, 588, 739 |
| BslFI | GGGAC | 2 cut(s) | 392, 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 551 |
| BsmAI | GTCTC | 2 cut(s) | 119, 502 |
| BsmFI | GGGAC | 2 cut(s) | 392, 453 |
| BsnI | GGCC | 6 cut(s) | 156, 242, 248, 263, 699, 730 |
| Bso31I | GGTCTC | 1 cut(s) | 502 |
| BsoBI | CYCGRG | 2 cut(s) | 505, 513 |
| Bsp143I | GATC | 4 cut(s) | 40, 193, 554, 786 |
| BspANI | GGCC | 6 cut(s) | 156, 242, 248, 263, 699, 730 |
| BspFNI | CGCG | 2 cut(s) | 90, 691 |
| BspLI | GGNNCC | 3 cut(s) | 408, 441, 661 |
| BspMI | ACCTGC | 1 cut(s) | 394 |
| BspOI | GCTAGC | 1 cut(s) | 431 |
| BspPI | GGATC | 3 cut(s) | 201, 562, 781 |
| BspTNI | GGTCTC | 1 cut(s) | 502 |
| BsrBI | CCGCTC | 3 cut(s) | 542, 602, 650 |
| BsrDI | GCAATG | 1 cut(s) | 499 |
| BsrFI | RCCGGY | 2 cut(s) | 587, 738 |
| BsrI | ACTGG | 2 cut(s) | 389, 615 |
| BssAI | RCCGGY | 2 cut(s) | 587, 738 |
| BssECI | CCNNGG | 6 cut(s) | 105, 115, 133, 243, 512, 545 |
| BssMI | GATC | 4 cut(s) | 40, 193, 554, 786 |
| Bst2UI | CCWGG | 7 cut(s) | 135, 153, 349, 547, 574, 696, 701 |
| Bst4CI | ACNGT | 1 cut(s) | 714 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 149, 183, 429, 728 |
| BstF5I | GGATG | 1 cut(s) | 624 |
| BstFNI | CGCG | 2 cut(s) | 90, 691 |
| BstH2I | RGCGCY | 1 cut(s) | 348 |
| BstHHI | GCGC | 3 cut(s) | 92, 231, 347 |
| BstKTI | GATC | 4 cut(s) | 43, 196, 557, 789 |
| BstMAI | GTCTC | 2 cut(s) | 119, 502 |
| BstMBI | GATC | 4 cut(s) | 40, 193, 554, 786 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 87, 153, 320, 363, 409, 593 |
| BstNI | CCWGG | 7 cut(s) | 135, 153, 349, 547, 574, 696, 701 |
| BstUI | CGCG | 2 cut(s) | 90, 691 |
| BstV1I | GCAGC | 2 cut(s) | 153, 399 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuI | GTATCC | 1 cut(s) | 283 |
| BsuRI | GGCC | 6 cut(s) | 156, 242, 248, 263, 699, 730 |
| BtsCI | GGATG | 1 cut(s) | 624 |
| BtsI | GCAGTG | 1 cut(s) | 488 |
| BtsIMutI | CAGTG | 2 cut(s) | 488, 873 |
| BveI | ACCTGC | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 149, 183, 429, 728 |
| CfoI | GCGC | 3 cut(s) | 92, 231, 347 |
| Cfr10I | RCCGGY | 2 cut(s) | 587, 738 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 406, 440, 703 |
| CseI | GACGC | 2 cut(s) | 32, 680 |
| Csp6I | GTAC | 2 cut(s) | 452, 570 |
| CviQI | GTAC | 2 cut(s) | 452, 570 |
| DpnI | GATC | 4 cut(s) | 42, 195, 556, 788 |
| DpnII | GATC | 4 cut(s) | 40, 193, 554, 786 |
| DraIII | CACNNNGTG | 2 cut(s) | 329, 854 |
| EaeI | YGGCCR | 2 cut(s) | 261, 728 |
| EciI | GGCGGA | 2 cut(s) | 37, 206 |
| Eco31I | GGTCTC | 1 cut(s) | 502 |
| Eco47I | GGWCC | 3 cut(s) | 406, 440, 703 |
| Eco88I | CYCGRG | 2 cut(s) | 505, 513 |
| EcoO109I | RGGNCCY | 1 cut(s) | 406 |
| EcoRII | CCWGG | 7 cut(s) | 133, 151, 347, 545, 572, 694, 699 |
| FaiI | YATR | 7 cut(s) | 177, 399, 401, 746, 773, 800, 802 |
| FaqI | GGGAC | 2 cut(s) | 392, 453 |
| FauI | CCCGC | 7 cut(s) | 8, 156, 284, 417, 424, 547, 592 |
| FauNDI | CATATG | 2 cut(s) | 399, 800 |
| Fnu4HI | GCNGC | 3 cut(s) | 142, 145, 413 |
| FokI | GGATG | 1 cut(s) | 631 |
| Fsp4HI | GCNGC | 3 cut(s) | 142, 145, 413 |
| FspBI | CTAG | 2 cut(s) | 428, 578 |
| GlaI | GCGC | 3 cut(s) | 91, 230, 346 |
| GluI | GCNGC | 3 cut(s) | 142, 145, 413 |
| GsaI | CCCAGC | 3 cut(s) | 74, 460, 667 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 348 |
| HaeIII | GGCC | 6 cut(s) | 156, 242, 248, 263, 699, 730 |
| HapII | CCGG | 5 cut(s) | 107, 117, 320, 588, 739 |
| HgaI | GACGC | 2 cut(s) | 32, 680 |
| HhaI | GCGC | 3 cut(s) | 92, 231, 347 |
| Hin6I | GCGC | 3 cut(s) | 90, 229, 345 |
| HinP1I | GCGC | 3 cut(s) | 90, 229, 345 |
| HincII | GTYRAC | 1 cut(s) | 112 |
| HindII | GTYRAC | 1 cut(s) | 112 |
| HinfI | GANTC | 4 cut(s) | 482, 653, 753, 836 |
| HpaII | CCGG | 5 cut(s) | 107, 117, 320, 588, 739 |
| HphI | GGTGA | 4 cut(s) | 91, 134, 602, 813 |
| Hpy166II | GTNNAC | 2 cut(s) | 112, 221 |
| Hpy188I | TCNGA | 2 cut(s) | 647, 736 |
| Hpy188III | TCNNGA | 4 cut(s) | 339, 507, 657, 813 |
| Hpy8I | GTNNAC | 2 cut(s) | 112, 221 |
| HpyAV | CCTTC | 3 cut(s) | 232, 801, 819 |
| HpyCH4III | ACNGT | 1 cut(s) | 714 |
| HpyCH4V | TGCA | 9 cut(s) | 366, 397, 403, 415, 493, 596, 622, 718, 748 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 87, 153, 320, 363, 409, 593 |
| HspAI | GCGC | 3 cut(s) | 90, 229, 345 |
| Kzo9I | GATC | 4 cut(s) | 40, 193, 554, 786 |
| LmnI | GCTCC | 2 cut(s) | 371, 547 |
| Lsp1109I | GCAGC | 2 cut(s) | 153, 399 |
| LweI | GCATC | 2 cut(s) | 583, 609 |
| MaeI | CTAG | 2 cut(s) | 428, 578 |
| MaeIII | GTNAC | 3 cut(s) | 336, 764, 819 |
| MalI | GATC | 4 cut(s) | 42, 195, 556, 788 |
| MbiI | CCGCTC | 3 cut(s) | 542, 602, 650 |
| MboI | GATC | 4 cut(s) | 40, 193, 554, 786 |
| MboII | GAAGA | 2 cut(s) | 32, 188 |
| MfeI | CAATTG | 1 cut(s) | 626 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MlsI | TGGCCA | 2 cut(s) | 263, 730 |
| MluCI | AATT | 2 cut(s) | 626, 719 |
| MluNI | TGGCCA | 2 cut(s) | 263, 730 |
| MlyI | GAGTC | 2 cut(s) | 491, 747 |
| MmeI | TCCRAC | 1 cut(s) | 447 |
| MnlI | CCTC | 9 cut(s) | 173, 178, 238, 298, 469, 520, 522, 545, 879 |
| Mox20I | TGGCCA | 2 cut(s) | 263, 730 |
| MscI | TGGCCA | 2 cut(s) | 263, 730 |
| MseI | TTAA | 2 cut(s) | 84, 641 |
| Msp20I | TGGCCA | 2 cut(s) | 263, 730 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 412, 565 |
| MspI | CCGG | 5 cut(s) | 107, 117, 320, 588, 739 |
| MunI | CAATTG | 1 cut(s) | 626 |
| MvaI | CCWGG | 7 cut(s) | 135, 153, 349, 547, 574, 696, 701 |
| MvnI | CGCG | 2 cut(s) | 90, 691 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 87, 153, 320, 363, 409, 593 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 320 |
| NdeI | CATATG | 2 cut(s) | 399, 800 |
| NdeII | GATC | 4 cut(s) | 40, 193, 554, 786 |
| NheI | GCTAGC | 1 cut(s) | 427 |
| NlaIV | GGNNCC | 3 cut(s) | 408, 441, 661 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 3 cut(s) | 336, 764, 819 |
| PaeR7I | CTCGAG | 1 cut(s) | 505 |
| PfeI | GAWTC | 2 cut(s) | 653, 836 |
| PkrI | GCNGC | 3 cut(s) | 143, 146, 414 |
| PleI | GAGTC | 2 cut(s) | 490, 747 |
| PpsI | GAGTC | 2 cut(s) | 490, 747 |
| PpuMI | RGGWCCY | 1 cut(s) | 406 |
| Psp5II | RGGWCCY | 1 cut(s) | 406 |
| Psp6I | CCWGG | 7 cut(s) | 133, 151, 347, 545, 572, 694, 699 |
| PspFI | CCCAGC | 3 cut(s) | 70, 456, 663 |
| PspGI | CCWGG | 7 cut(s) | 133, 151, 347, 545, 572, 694, 699 |
| PspN4I | GGNNCC | 3 cut(s) | 408, 441, 661 |
| PspPI | GGNCC | 4 cut(s) | 247, 406, 440, 703 |
| PspPPI | RGGWCCY | 1 cut(s) | 406 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 2 cut(s) | 453, 571 |
| RsaNI | GTAC | 2 cut(s) | 452, 570 |
| SaqAI | TTAA | 2 cut(s) | 84, 641 |
| SatI | GCNGC | 3 cut(s) | 142, 145, 413 |
| Sau3AI | GATC | 4 cut(s) | 40, 193, 554, 786 |
| Sau96I | GGNCC | 4 cut(s) | 247, 406, 440, 703 |
| SchI | GAGTC | 2 cut(s) | 491, 747 |
| SfaNI | GCATC | 2 cut(s) | 583, 609 |
| Sfr274I | CTCGAG | 1 cut(s) | 505 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SinI | GGWCC | 3 cut(s) | 406, 440, 703 |
| SlaI | CTCGAG | 1 cut(s) | 505 |
| SmlI | CTYRAG | 1 cut(s) | 505 |
| SmoI | CTYRAG | 1 cut(s) | 505 |
| Sse9I | AATT | 2 cut(s) | 626, 719 |
| SspMI | CTAG | 2 cut(s) | 428, 578 |
| TaaI | ACNGT | 1 cut(s) | 714 |
| TaqI | TCGA | 2 cut(s) | 506, 839 |
| TasI | AATT | 2 cut(s) | 626, 719 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TfiI | GAWTC | 2 cut(s) | 653, 836 |
| Tru1I | TTAA | 2 cut(s) | 84, 641 |
| Tru9I | TTAA | 2 cut(s) | 84, 641 |
| TscAI | CASTG | 2 cut(s) | 495, 873 |
| TseFI | GTSAC | 3 cut(s) | 336, 764, 819 |
| TseI | GCWGC | 2 cut(s) | 141, 412 |
| Tsp45I | GTSAC | 3 cut(s) | 336, 764, 819 |
| TspRI | CASTG | 2 cut(s) | 495, 873 |
| VpaK11BI | GGWCC | 3 cut(s) | 406, 440, 703 |
| XhoI | CTCGAG | 1 cut(s) | 505 |
| XspI | CTAG | 2 cut(s) | 428, 578 |