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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 440 |
| Acc65I | GGTACC | 2 cut(s) | 426, 452 |
| AccB1I | GGYRCC | 2 cut(s) | 426, 452 |
| AccII | CGCG | 3 cut(s) | 74, 140, 403 |
| AclWI | GGATC | 2 cut(s) | 48, 231 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AcuI | CTGAAG | 2 cut(s) | 374, 518 |
| AfaI | GTAC | 3 cut(s) | 428, 433, 454 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 2 cut(s) | 159, 219 |
| AluBI | AGCT | 2 cut(s) | 244, 386 |
| AluI | AGCT | 2 cut(s) | 244, 386 |
| Alw21I | GWGCWC | 1 cut(s) | 410 |
| AlwI | GGATC | 2 cut(s) | 48, 231 |
| AoxI | GGCC | 3 cut(s) | 189, 217, 309 |
| ApeKI | GCWGC | 1 cut(s) | 182 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| Asp718I | GGTACC | 2 cut(s) | 426, 452 |
| AspLEI | GCGC | 2 cut(s) | 140, 364 |
| AspS9I | GGNCC | 5 cut(s) | 163, 190, 217, 256, 310 |
| AsuC2I | CCSGG | 1 cut(s) | 198 |
| AvaII | GGWCC | 2 cut(s) | 163, 256 |
| BanI | GGYRCC | 2 cut(s) | 426, 452 |
| Bbv12I | GWGCWC | 1 cut(s) | 410 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 194 |
| BccI | CCATC | 2 cut(s) | 66, 220 |
| BceAI | ACGGC | 1 cut(s) | 25 |
| BciT130I | CCWGG | 2 cut(s) | 161, 221 |
| BcnI | CCSGG | 1 cut(s) | 198 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BfuAI | ACCTGC | 1 cut(s) | 440 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 216 |
| BisI | GCNGC | 3 cut(s) | 75, 183, 231 |
| BlsI | GCNGC | 3 cut(s) | 76, 184, 232 |
| Bme1390I | CCNGG | 3 cut(s) | 161, 198, 221 |
| Bme18I | GGWCC | 2 cut(s) | 163, 256 |
| BmgT120I | GGNCC | 5 cut(s) | 163, 190, 217, 256, 310 |
| BmiI | GGNNCC | 4 cut(s) | 144, 165, 428, 454 |
| BmrFI | CCNGG | 3 cut(s) | 161, 198, 221 |
| BmsI | GCATC | 1 cut(s) | 436 |
| BpmI | CTGGAG | 1 cut(s) | 386 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 198 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 148, 159 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| Bse118I | RCCGGY | 1 cut(s) | 415 |
| Bse1I | ACTGG | 1 cut(s) | 255 |
| Bse3DI | GCAATG | 1 cut(s) | 561 |
| BseBI | CCWGG | 2 cut(s) | 161, 221 |
| BseDI | CCNNGG | 3 cut(s) | 72, 148, 159 |
| BseGI | GGATG | 2 cut(s) | 58, 155 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseMI | GCAATG | 1 cut(s) | 561 |
| BseMII | CTCAG | 2 cut(s) | 18, 401 |
| BseNI | ACTGG | 1 cut(s) | 255 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 1 cut(s) | 194 |
| BseYI | CCCAGC | 3 cut(s) | 88, 134, 382 |
| Bsh1236I | CGCG | 3 cut(s) | 74, 140, 403 |
| Bsh1285I | CGRYCG | 1 cut(s) | 197 |
| BshFI | GGCC | 3 cut(s) | 191, 219, 311 |
| BshNI | GGYRCC | 2 cut(s) | 426, 452 |
| BsiEI | CGRYCG | 1 cut(s) | 197 |
| BsiHKAI | GWGCWC | 1 cut(s) | 410 |
| BsiSI | CCGG | 3 cut(s) | 125, 197, 416 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 3 cut(s) | 191, 219, 311 |
| Bsp1286I | GDGCHC | 1 cut(s) | 410 |
| Bsp143I | GATC | 2 cut(s) | 40, 223 |
| BspANI | GGCC | 3 cut(s) | 191, 219, 311 |
| BspCNI | CTCAG | 2 cut(s) | 19, 400 |
| BspFNI | CGCG | 3 cut(s) | 74, 140, 403 |
| BspLI | GGNNCC | 4 cut(s) | 144, 165, 428, 454 |
| BspMI | ACCTGC | 1 cut(s) | 440 |
| BspPI | GGATC | 2 cut(s) | 48, 231 |
| BspT107I | GGYRCC | 2 cut(s) | 426, 452 |
| BsrDI | GCAATG | 1 cut(s) | 561 |
| BsrFI | RCCGGY | 1 cut(s) | 415 |
| BsrI | ACTGG | 1 cut(s) | 255 |
| BssAI | RCCGGY | 1 cut(s) | 415 |
| BssECI | CCNNGG | 3 cut(s) | 72, 148, 159 |
| BssMI | GATC | 2 cut(s) | 40, 223 |
| BssT1I | CCWWGG | 1 cut(s) | 148 |
| Bst2UI | CCWGG | 2 cut(s) | 161, 221 |
| Bst4CI | ACNGT | 3 cut(s) | 107, 206, 431 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 246, 395 |
| BstDEI | CTNAG | 2 cut(s) | 27, 387 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 2 cut(s) | 58, 155 |
| BstFNI | CGCG | 3 cut(s) | 74, 140, 403 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 2 cut(s) | 140, 364 |
| BstKTI | GATC | 2 cut(s) | 43, 226 |
| BstMBI | GATC | 2 cut(s) | 40, 223 |
| BstMCI | CGRYCG | 1 cut(s) | 197 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 188, 207, 216, 368, 383, 455 |
| BstNI | CCWGG | 2 cut(s) | 161, 221 |
| BstSCI | CCNGG | 3 cut(s) | 159, 196, 219 |
| BstUI | CGCG | 3 cut(s) | 74, 140, 403 |
| BstV1I | GCAGC | 1 cut(s) | 194 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| BsuRI | GGCC | 3 cut(s) | 191, 219, 311 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsCI | GGATG | 2 cut(s) | 58, 155 |
| BtsI | GCAGTG | 1 cut(s) | 19 |
| BtsIMutI | CAGTG | 2 cut(s) | 19, 248 |
| BveI | ACCTGC | 1 cut(s) | 440 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 246, 395 |
| CfoI | GCGC | 2 cut(s) | 140, 364 |
| Cfr10I | RCCGGY | 1 cut(s) | 415 |
| Cfr13I | GGNCC | 5 cut(s) | 163, 190, 217, 256, 310 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| Csp6I | GTAC | 3 cut(s) | 427, 432, 453 |
| CviJI | RGCY | 8 cut(s) | 77, 153, 191, 219, 244, 311, 326, 386 |
| CviKI_1 | RGCY | 8 cut(s) | 77, 153, 191, 219, 244, 311, 326, 386 |
| CviQI | GTAC | 3 cut(s) | 427, 432, 453 |
| DdeI | CTNAG | 2 cut(s) | 27, 387 |
| DpnI | GATC | 2 cut(s) | 42, 225 |
| DpnII | GATC | 2 cut(s) | 40, 223 |
| EciI | GGCGGA | 2 cut(s) | 269, 434 |
| Eco130I | CCWWGG | 1 cut(s) | 148 |
| Eco47I | GGWCC | 2 cut(s) | 163, 256 |
| Eco57I | CTGAAG | 2 cut(s) | 374, 518 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 163 |
| EcoRII | CCWGG | 2 cut(s) | 159, 219 |
| EcoT14I | CCWWGG | 1 cut(s) | 148 |
| ErhI | CCWWGG | 1 cut(s) | 148 |
| FaiI | YATR | 4 cut(s) | 236, 240, 441, 570 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| FauI | CCCGC | 1 cut(s) | 79 |
| Fnu4HI | GCNGC | 3 cut(s) | 75, 183, 231 |
| FokI | GGATG | 2 cut(s) | 45, 142 |
| Fsp4HI | GCNGC | 3 cut(s) | 75, 183, 231 |
| GlaI | GCGC | 2 cut(s) | 139, 363 |
| GluI | GCNGC | 3 cut(s) | 75, 183, 231 |
| GsaI | CCCAGC | 3 cut(s) | 92, 138, 386 |
| GsuI | CTGGAG | 1 cut(s) | 386 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 3 cut(s) | 191, 219, 311 |
| HapII | CCGG | 3 cut(s) | 125, 197, 416 |
| HhaI | GCGC | 2 cut(s) | 140, 364 |
| Hin6I | GCGC | 2 cut(s) | 138, 362 |
| HinP1I | GCGC | 2 cut(s) | 138, 362 |
| HincII | GTYRAC | 2 cut(s) | 130, 489 |
| HindII | GTYRAC | 2 cut(s) | 130, 489 |
| HinfI | GANTC | 1 cut(s) | 172 |
| HpaII | CCGG | 3 cut(s) | 125, 197, 416 |
| Hpy166II | GTNNAC | 3 cut(s) | 103, 130, 489 |
| Hpy188I | TCNGA | 2 cut(s) | 171, 390 |
| Hpy8I | GTNNAC | 3 cut(s) | 103, 130, 489 |
| Hpy99I | CGWCG | 1 cut(s) | 350 |
| HpyAV | CCTTC | 3 cut(s) | 295, 352, 493 |
| HpyCH4III | ACNGT | 3 cut(s) | 107, 206, 431 |
| HpyCH4V | TGCA | 2 cut(s) | 110, 449 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 188, 207, 216, 368, 383, 455 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 387 |
| HspAI | GCGC | 2 cut(s) | 138, 362 |
| KpnI | GGTACC | 2 cut(s) | 430, 456 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 40, 223 |
| LmnI | GCTCC | 2 cut(s) | 31, 405 |
| Lsp1109I | GCAGC | 1 cut(s) | 194 |
| LweI | GCATC | 1 cut(s) | 436 |
| MalI | GATC | 2 cut(s) | 42, 225 |
| MboI | GATC | 2 cut(s) | 40, 223 |
| MboII | GAAGA | 1 cut(s) | 367 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 1 cut(s) | 410 |
| MluCI | AATT | 2 cut(s) | 341, 533 |
| MlyI | GAGTC | 1 cut(s) | 166 |
| MnlI | CCTC | 7 cut(s) | 22, 177, 181, 269, 301, 361, 502 |
| MseI | TTAA | 4 cut(s) | 84, 96, 542, 574 |
| MslI | CAYNNNNRTG | 3 cut(s) | 332, 444, 527 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 92 |
| MspI | CCGG | 3 cut(s) | 125, 197, 416 |
| MspR9I | CCNGG | 3 cut(s) | 161, 198, 221 |
| MvaI | CCWGG | 2 cut(s) | 161, 221 |
| MvnI | CGCG | 3 cut(s) | 74, 140, 403 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 188, 207, 216, 368, 383, 455 |
| NciI | CCSGG | 1 cut(s) | 198 |
| NdeII | GATC | 2 cut(s) | 40, 223 |
| NlaIV | GGNNCC | 4 cut(s) | 144, 165, 428, 454 |
| PkrI | GCNGC | 3 cut(s) | 76, 184, 232 |
| PleI | GAGTC | 1 cut(s) | 166 |
| PpsI | GAGTC | 1 cut(s) | 166 |
| PpuMI | RGGWCCY | 1 cut(s) | 163 |
| Psp5II | RGGWCCY | 1 cut(s) | 163 |
| Psp6I | CCWGG | 2 cut(s) | 159, 219 |
| PspFI | CCCAGC | 3 cut(s) | 88, 134, 382 |
| PspGI | CCWGG | 2 cut(s) | 159, 219 |
| PspN4I | GGNNCC | 4 cut(s) | 144, 165, 428, 454 |
| PspPI | GGNCC | 5 cut(s) | 163, 190, 217, 256, 310 |
| PspPPI | RGGWCCY | 1 cut(s) | 163 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| RsaI | GTAC | 3 cut(s) | 428, 433, 454 |
| RsaNI | GTAC | 3 cut(s) | 427, 432, 453 |
| RseI | CAYNNNNRTG | 3 cut(s) | 332, 444, 527 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 542, 574 |
| SatI | GCNGC | 3 cut(s) | 75, 183, 231 |
| Sau3AI | GATC | 2 cut(s) | 40, 223 |
| Sau96I | GGNCC | 5 cut(s) | 163, 190, 217, 256, 310 |
| SchI | GAGTC | 1 cut(s) | 166 |
| ScrFI | CCNGG | 3 cut(s) | 161, 198, 221 |
| SduI | GDGCHC | 1 cut(s) | 410 |
| SetI | ASST | 6 cut(s) | 165, 246, 261, 388, 454, 494 |
| SfaNI | GCATC | 1 cut(s) | 436 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 2 cut(s) | 163, 256 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 332, 444, 527 |
| Sse9I | AATT | 2 cut(s) | 341, 533 |
| StyD4I | CCNGG | 3 cut(s) | 159, 196, 219 |
| StyI | CCWWGG | 1 cut(s) | 148 |
| TaaI | ACNGT | 3 cut(s) | 107, 206, 431 |
| TaqI | TCGA | 1 cut(s) | 345 |
| TasI | AATT | 2 cut(s) | 341, 533 |
| TauI | GCSGC | 2 cut(s) | 77, 233 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 542, 574 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 542, 574 |
| TscAI | CASTG | 2 cut(s) | 19, 255 |
| TseI | GCWGC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 2 cut(s) | 545, 550 |
| TspRI | CASTG | 2 cut(s) | 19, 255 |
| VpaK11BI | GGWCC | 2 cut(s) | 163, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 1 cut(s) | 341 |