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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 208 |
| AccB1I | GGYRCC | 1 cut(s) | 208 |
| AccBSI | CCGCTC | 2 cut(s) | 333, 414 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AciI | CCGC | 7 cut(s) | 201, 239, 286, 308, 331, 377, 412 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AcuI | CTGAAG | 1 cut(s) | 374 |
| AfaI | GTAC | 3 cut(s) | 210, 216, 237 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 31, 85, 197, 377 |
| AjnI | CCWGG | 2 cut(s) | 84, 372 |
| AluBI | AGCT | 2 cut(s) | 256, 383 |
| AluI | AGCT | 2 cut(s) | 256, 383 |
| Alw21I | GWGCWC | 2 cut(s) | 176, 385 |
| AoxI | GGCC | 4 cut(s) | 75, 90, 268, 408 |
| ApaI | GGGCCC | 2 cut(s) | 272, 412 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| Asp718I | GGTACC | 1 cut(s) | 208 |
| AspLEI | GCGC | 3 cut(s) | 48, 130, 154 |
| AspS9I | GGNCC | 6 cut(s) | 22, 76, 268, 269, 408, 409 |
| AsuHPI | GGTGA | 1 cut(s) | 431 |
| AvaII | GGWCC | 1 cut(s) | 22 |
| BaeGI | GKGCMC | 2 cut(s) | 272, 412 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BanI | GGYRCC | 1 cut(s) | 208 |
| BanII | GRGCYC | 3 cut(s) | 272, 385, 412 |
| Bbv12I | GWGCWC | 2 cut(s) | 176, 385 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BciT130I | CCWGG | 2 cut(s) | 86, 374 |
| BfaI | CTAG | 3 cut(s) | 342, 347, 401 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| Bme1390I | CCNGG | 2 cut(s) | 86, 374 |
| Bme18I | GGWCC | 1 cut(s) | 22 |
| BmgT120I | GGNCC | 6 cut(s) | 22, 76, 268, 269, 408, 409 |
| BmiI | GGNNCC | 3 cut(s) | 210, 270, 410 |
| BmrFI | CCNGG | 2 cut(s) | 86, 374 |
| BmsI | GCATC | 2 cut(s) | 219, 368 |
| BpmI | CTGGAG | 1 cut(s) | 152 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| BsaJI | CCNNGG | 1 cut(s) | 372 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 31, 85, 197, 377 |
| Bse118I | RCCGGY | 1 cut(s) | 10 |
| BseBI | CCWGG | 2 cut(s) | 86, 374 |
| BseDI | CCNNGG | 1 cut(s) | 372 |
| BseGI | GGATG | 1 cut(s) | 359 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 31, 85, 197, 377 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseSI | GKGCMC | 2 cut(s) | 272, 412 |
| BseYI | CCCAGC | 1 cut(s) | 148 |
| BshFI | GGCC | 4 cut(s) | 77, 92, 270, 410 |
| BshNI | GGYRCC | 1 cut(s) | 208 |
| BsiHKAI | GWGCWC | 2 cut(s) | 176, 385 |
| BsiSI | CCGG | 1 cut(s) | 11 |
| BslFI | GGGAC | 1 cut(s) | 354 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 31, 85, 197, 377 |
| BsmFI | GGGAC | 1 cut(s) | 354 |
| BsnI | GGCC | 4 cut(s) | 77, 92, 270, 410 |
| Bsp120I | GGGCCC | 2 cut(s) | 268, 408 |
| Bsp1286I | GDGCHC | 4 cut(s) | 176, 272, 385, 412 |
| BspACI | CCGC | 7 cut(s) | 201, 239, 286, 308, 331, 377, 412 |
| BspANI | GGCC | 4 cut(s) | 77, 92, 270, 410 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspLI | GGNNCC | 3 cut(s) | 210, 270, 410 |
| BspT107I | GGYRCC | 1 cut(s) | 208 |
| BsrBI | CCGCTC | 2 cut(s) | 333, 414 |
| BsrFI | RCCGGY | 1 cut(s) | 10 |
| BssAI | RCCGGY | 1 cut(s) | 10 |
| BssECI | CCNNGG | 1 cut(s) | 372 |
| Bst2UI | CCWGG | 2 cut(s) | 86, 374 |
| BstC8I | GCNNGC | 3 cut(s) | 12, 161, 412 |
| BstDEI | CTNAG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstF5I | GGATG | 1 cut(s) | 359 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 3 cut(s) | 48, 130, 154 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 134, 149, 160, 238, 389, 411 |
| BstNI | CCWGG | 2 cut(s) | 86, 374 |
| BstSCI | CCNGG | 2 cut(s) | 84, 372 |
| BstSLI | GKGCMC | 2 cut(s) | 272, 412 |
| BsuRI | GGCC | 4 cut(s) | 77, 92, 270, 410 |
| BtsCI | GGATG | 1 cut(s) | 359 |
| Cac8I | GCNNGC | 3 cut(s) | 12, 161, 412 |
| CfoI | GCGC | 3 cut(s) | 48, 130, 154 |
| Cfr10I | RCCGGY | 1 cut(s) | 10 |
| Cfr13I | GGNCC | 6 cut(s) | 22, 76, 268, 269, 408, 409 |
| CsiI | ACCWGGT | 1 cut(s) | 84 |
| Csp6I | GTAC | 3 cut(s) | 209, 215, 236 |
| CviJI | RGCY | 9 cut(s) | 10, 71, 77, 92, 256, 270, 346, 383, 410 |
| CviKI_1 | RGCY | 9 cut(s) | 10, 71, 77, 92, 256, 270, 346, 383, 410 |
| CviQI | GTAC | 3 cut(s) | 209, 215, 236 |
| DdeI | CTNAG | 1 cut(s) | 72 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| EciI | GGCGGA | 1 cut(s) | 216 |
| Ecl136II | GAGCTC | 1 cut(s) | 383 |
| Eco24I | GRGCYC | 3 cut(s) | 272, 385, 412 |
| Eco47I | GGWCC | 1 cut(s) | 22 |
| Eco53kI | GAGCTC | 1 cut(s) | 383 |
| Eco57I | CTGAAG | 1 cut(s) | 374 |
| EcoICRI | GAGCTC | 1 cut(s) | 383 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoO109I | RGGNCCY | 1 cut(s) | 408 |
| EcoRII | CCWGG | 2 cut(s) | 84, 372 |
| EcoT38I | GRGCYC | 3 cut(s) | 272, 385, 412 |
| FaiI | YATR | 3 cut(s) | 6, 30, 224 |
| FaqI | GGGAC | 1 cut(s) | 354 |
| FauI | CCCGC | 3 cut(s) | 293, 370, 419 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| FokI | GGATG | 1 cut(s) | 346 |
| FriOI | GRGCYC | 3 cut(s) | 272, 385, 412 |
| FspBI | CTAG | 3 cut(s) | 342, 347, 401 |
| GlaI | GCGC | 3 cut(s) | 47, 129, 153 |
| GsaI | CCCAGC | 1 cut(s) | 152 |
| GsuI | CTGGAG | 1 cut(s) | 152 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 4 cut(s) | 77, 92, 270, 410 |
| HapII | CCGG | 1 cut(s) | 11 |
| HhaI | GCGC | 3 cut(s) | 48, 130, 154 |
| Hin6I | GCGC | 3 cut(s) | 46, 128, 152 |
| HinP1I | GCGC | 3 cut(s) | 46, 128, 152 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 2 cut(s) | 311, 395 |
| HpaII | CCGG | 1 cut(s) | 11 |
| HphI | GGTGA | 1 cut(s) | 431 |
| Hpy166II | GTNNAC | 2 cut(s) | 22, 188 |
| Hpy188I | TCNGA | 1 cut(s) | 354 |
| Hpy8I | GTNNAC | 2 cut(s) | 22, 188 |
| HpyAV | CCTTC | 2 cut(s) | 61, 349 |
| HpyCH4IV | ACGT | 1 cut(s) | 181 |
| HpyCH4V | TGCA | 1 cut(s) | 232 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 134, 149, 160, 238, 389, 411 |
| HpyF3I | CTNAG | 1 cut(s) | 72 |
| HpySE526I | ACGT | 1 cut(s) | 181 |
| HspAI | GCGC | 3 cut(s) | 46, 128, 152 |
| KpnI | GGTACC | 1 cut(s) | 212 |
| KroI | GCCGGC | 1 cut(s) | 10 |
| KroNI | GCCGGC | 1 cut(s) | 12 |
| LmnI | GCTCC | 3 cut(s) | 43, 171, 380 |
| LweI | GCATC | 2 cut(s) | 219, 368 |
| MabI | ACCWGGT | 1 cut(s) | 84 |
| MaeI | CTAG | 3 cut(s) | 342, 347, 401 |
| MaeII | ACGT | 1 cut(s) | 181 |
| MaeIII | GTNAC | 2 cut(s) | 312, 419 |
| MbiI | CCGCTC | 2 cut(s) | 333, 414 |
| MhlI | GDGCHC | 4 cut(s) | 176, 272, 385, 412 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluCI | AATT | 1 cut(s) | 107 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MlyI | GAGTC | 1 cut(s) | 320 |
| MnlI | CCTC | 4 cut(s) | 35, 67, 127, 285 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MroNI | GCCGGC | 1 cut(s) | 10 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MslI | CAYNNNNRTG | 2 cut(s) | 98, 227 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspI | CCGG | 1 cut(s) | 11 |
| MspR9I | CCNGG | 2 cut(s) | 86, 374 |
| MvaI | CCWGG | 2 cut(s) | 86, 374 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 134, 149, 160, 238, 389, 411 |
| NaeI | GCCGGC | 1 cut(s) | 12 |
| NgoMIV | GCCGGC | 1 cut(s) | 10 |
| NlaIV | GGNNCC | 3 cut(s) | 210, 270, 410 |
| NmuCI | GTSAC | 2 cut(s) | 312, 419 |
| PdiI | GCCGGC | 1 cut(s) | 12 |
| PfeI | GAWTC | 1 cut(s) | 395 |
| PflFI | GACNNNGTC | 1 cut(s) | 183 |
| PleI | GAGTC | 1 cut(s) | 319 |
| PpsI | GAGTC | 1 cut(s) | 319 |
| Psp124BI | GAGCTC | 1 cut(s) | 385 |
| Psp6I | CCWGG | 2 cut(s) | 84, 372 |
| PspFI | CCCAGC | 1 cut(s) | 148 |
| PspGI | CCWGG | 2 cut(s) | 84, 372 |
| PspN4I | GGNNCC | 3 cut(s) | 210, 270, 410 |
| PspOMI | GGGCCC | 2 cut(s) | 268, 408 |
| PspPI | GGNCC | 6 cut(s) | 22, 76, 268, 269, 408, 409 |
| PsyI | GACNNNGTC | 1 cut(s) | 183 |
| RsaI | GTAC | 3 cut(s) | 210, 216, 237 |
| RsaNI | GTAC | 3 cut(s) | 209, 215, 236 |
| RseI | CAYNNNNRTG | 2 cut(s) | 98, 227 |
| SacI | GAGCTC | 1 cut(s) | 385 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| Sau96I | GGNCC | 6 cut(s) | 22, 76, 268, 269, 408, 409 |
| SchI | GAGTC | 1 cut(s) | 320 |
| ScrFI | CCNGG | 2 cut(s) | 86, 374 |
| SduI | GDGCHC | 4 cut(s) | 176, 272, 385, 412 |
| SetI | ASST | 9 cut(s) | 27, 90, 184, 258, 277, 352, 368, 385, 431 |
| SexAI | ACCWGGT | 1 cut(s) | 84 |
| SfaNI | GCATC | 2 cut(s) | 219, 368 |
| SinI | GGWCC | 1 cut(s) | 22 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 98, 227 |
| Sse9I | AATT | 1 cut(s) | 107 |
| SsiI | CCGC | 7 cut(s) | 201, 239, 286, 308, 331, 377, 412 |
| SspMI | CTAG | 3 cut(s) | 342, 347, 401 |
| SstI | GAGCTC | 1 cut(s) | 385 |
| StyD4I | CCNGG | 2 cut(s) | 84, 372 |
| TaiI | ACGT | 1 cut(s) | 184 |
| TaqI | TCGA | 2 cut(s) | 111, 187 |
| TasI | AATT | 1 cut(s) | 107 |
| TfiI | GAWTC | 1 cut(s) | 395 |
| TseFI | GTSAC | 2 cut(s) | 312, 419 |
| Tsp45I | GTSAC | 2 cut(s) | 312, 419 |
| Tth111I | GACNNNGTC | 1 cut(s) | 183 |
| VpaK11BI | GGWCC | 1 cut(s) | 22 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 3 cut(s) | 342, 347, 401 |