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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 38, 150 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 1 cut(s) | 243 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 238 |
| AluBI | AGCT | 1 cut(s) | 163 |
| AluI | AGCT | 1 cut(s) | 163 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 4 cut(s) | 111, 222, 228, 243 |
| AspLEI | GCGC | 2 cut(s) | 283, 307 |
| AspS9I | GGNCC | 2 cut(s) | 112, 229 |
| AsuC2I | CCSGG | 2 cut(s) | 107, 120 |
| BalI | TGGCCA | 1 cut(s) | 245 |
| BccI | CCATC | 1 cut(s) | 66 |
| BcnI | CCSGG | 2 cut(s) | 107, 120 |
| BfoI | RGCGCY | 1 cut(s) | 284 |
| BglII | AGATCT | 1 cut(s) | 142 |
| BisI | GCNGC | 1 cut(s) | 150 |
| BlsI | GCNGC | 1 cut(s) | 151 |
| Bme1390I | CCNGG | 2 cut(s) | 107, 120 |
| BmgT120I | GGNCC | 2 cut(s) | 112, 229 |
| BmrFI | CCNGG | 2 cut(s) | 107, 120 |
| BpmI | CTGGAG | 1 cut(s) | 305 |
| BpuMI | CCSGG | 2 cut(s) | 107, 120 |
| BsaJI | CCNNGG | 1 cut(s) | 225 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 238 |
| BseDI | CCNNGG | 1 cut(s) | 225 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 238 |
| BseYI | CCCAGC | 2 cut(s) | 88, 301 |
| Bsh1285I | CGRYCG | 1 cut(s) | 119 |
| BshFI | GGCC | 4 cut(s) | 113, 224, 230, 245 |
| BsiEI | CGRYCG | 1 cut(s) | 119 |
| BsiSI | CCGG | 2 cut(s) | 107, 119 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 238 |
| BsnI | GGCC | 4 cut(s) | 113, 224, 230, 245 |
| Bsp143I | GATC | 2 cut(s) | 40, 142 |
| BspACI | CCGC | 2 cut(s) | 38, 150 |
| BspANI | GGCC | 4 cut(s) | 113, 224, 230, 245 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BssECI | CCNNGG | 1 cut(s) | 225 |
| BssMI | GATC | 2 cut(s) | 40, 142 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 165, 314 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstH2I | RGCGCY | 1 cut(s) | 284 |
| BstHHI | GCGC | 2 cut(s) | 283, 307 |
| BstKTI | GATC | 2 cut(s) | 43, 145 |
| BstMBI | GATC | 2 cut(s) | 40, 142 |
| BstMCI | CGRYCG | 1 cut(s) | 119 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 129, 287, 302, 313 |
| BstSCI | CCNGG | 2 cut(s) | 105, 118 |
| BstX2I | RGATCY | 1 cut(s) | 142 |
| BstYI | RGATCY | 1 cut(s) | 142 |
| BsuRI | GGCC | 4 cut(s) | 113, 224, 230, 245 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 1 cut(s) | 24 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 165, 314 |
| CfoI | GCGC | 2 cut(s) | 283, 307 |
| Cfr13I | GGNCC | 2 cut(s) | 112, 229 |
| CviJI | RGCY | 6 cut(s) | 113, 163, 187, 224, 230, 245 |
| CviKI_1 | RGCY | 6 cut(s) | 113, 163, 187, 224, 230, 245 |
| DpnI | GATC | 2 cut(s) | 42, 144 |
| DpnII | GATC | 2 cut(s) | 40, 142 |
| EaeI | YGGCCR | 1 cut(s) | 243 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| FaiI | YATR | 3 cut(s) | 155, 157, 159 |
| Fnu4HI | GCNGC | 1 cut(s) | 150 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 1 cut(s) | 150 |
| GlaI | GCGC | 2 cut(s) | 282, 306 |
| GluI | GCNGC | 1 cut(s) | 150 |
| GsaI | CCCAGC | 2 cut(s) | 92, 305 |
| GsuI | CTGGAG | 1 cut(s) | 305 |
| HaeII | RGCGCY | 1 cut(s) | 284 |
| HaeIII | GGCC | 4 cut(s) | 113, 224, 230, 245 |
| HapII | CCGG | 2 cut(s) | 107, 119 |
| HhaI | GCGC | 2 cut(s) | 283, 307 |
| Hin6I | GCGC | 2 cut(s) | 281, 305 |
| HinP1I | GCGC | 2 cut(s) | 281, 305 |
| HincII | GTYRAC | 3 cut(s) | 52, 85, 103 |
| HindII | GTYRAC | 3 cut(s) | 52, 85, 103 |
| HpaI | GTTAAC | 2 cut(s) | 85, 103 |
| HpaII | CCGG | 2 cut(s) | 107, 119 |
| Hpy166II | GTNNAC | 3 cut(s) | 52, 85, 103 |
| Hpy188III | TCNNGA | 1 cut(s) | 258 |
| Hpy8I | GTNNAC | 3 cut(s) | 52, 85, 103 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 129, 287, 302, 313 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| HspAI | GCGC | 2 cut(s) | 281, 305 |
| KspAI | GTTAAC | 2 cut(s) | 85, 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 142 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LpnPI | CCDG | 8 cut(s) | 102, 120, 132, 149, 178, 224, 269, 315 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MaeIII | GTNAC | 1 cut(s) | 73 |
| MalI | GATC | 2 cut(s) | 42, 144 |
| MboI | GATC | 2 cut(s) | 40, 142 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MflI | RGATCY | 1 cut(s) | 142 |
| MlsI | TGGCCA | 1 cut(s) | 245 |
| MluNI | TGGCCA | 1 cut(s) | 245 |
| MnlI | CCTC | 5 cut(s) | 10, 103, 188, 220, 280 |
| Mox20I | TGGCCA | 1 cut(s) | 245 |
| MscI | TGGCCA | 1 cut(s) | 245 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MslI | CAYNNNNRTG | 1 cut(s) | 251 |
| Msp20I | TGGCCA | 1 cut(s) | 245 |
| MspI | CCGG | 2 cut(s) | 107, 119 |
| MspR9I | CCNGG | 2 cut(s) | 107, 120 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 129, 287, 302, 313 |
| NciI | CCSGG | 2 cut(s) | 107, 120 |
| NdeII | GATC | 2 cut(s) | 40, 142 |
| NmeAIII | GCCGAG | 1 cut(s) | 250 |
| NmuCI | GTSAC | 1 cut(s) | 73 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 1 cut(s) | 151 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| PspFI | CCCAGC | 2 cut(s) | 88, 301 |
| PspPI | GGNCC | 2 cut(s) | 112, 229 |
| PsuI | RGATCY | 1 cut(s) | 142 |
| RseI | CAYNNNNRTG | 1 cut(s) | 251 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 1 cut(s) | 150 |
| Sau3AI | GATC | 2 cut(s) | 40, 142 |
| Sau96I | GGNCC | 2 cut(s) | 112, 229 |
| ScrFI | CCNGG | 2 cut(s) | 107, 120 |
| SetI | ASST | 5 cut(s) | 84, 102, 165, 180, 243 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 251 |
| SsiI | CCGC | 2 cut(s) | 38, 150 |
| StyD4I | CCNGG | 2 cut(s) | 105, 118 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TauI | GCSGC | 1 cut(s) | 152 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TscAI | CASTG | 1 cut(s) | 31 |
| TseFI | GTSAC | 1 cut(s) | 73 |
| Tsp45I | GTSAC | 1 cut(s) | 73 |
| TspGWI | ACGGA | 1 cut(s) | 37 |
| TspRI | CASTG | 1 cut(s) | 31 |