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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 90 |
| AciI | CCGC | 9 cut(s) | 15, 38, 48, 72, 88, 107, 191, 316, 382 |
| AclWI | GGATC | 2 cut(s) | 168, 201 |
| AfaI | GTAC | 3 cut(s) | 176, 368, 380 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 116 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 2 cut(s) | 133, 156 |
| AleI | CACNNNNGTG | 1 cut(s) | 349 |
| AlwI | GGATC | 2 cut(s) | 168, 201 |
| AoxI | GGCC | 4 cut(s) | 154, 246, 306, 358 |
| ApeKI | GCWGC | 2 cut(s) | 138, 141 |
| AspLEI | GCGC | 2 cut(s) | 92, 231 |
| AspS9I | GGNCC | 2 cut(s) | 247, 358 |
| AsuC2I | CCSGG | 1 cut(s) | 117 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 160 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| BbvI | GCAGC | 2 cut(s) | 150, 153 |
| BccI | CCATC | 1 cut(s) | 342 |
| BciT130I | CCWGG | 2 cut(s) | 135, 158 |
| BcnI | CCSGG | 1 cut(s) | 117 |
| BfaI | CTAG | 1 cut(s) | 302 |
| BisI | GCNGC | 2 cut(s) | 139, 142 |
| BlsI | GCNGC | 2 cut(s) | 140, 143 |
| Bme1390I | CCNGG | 3 cut(s) | 117, 135, 158 |
| BmgT120I | GGNCC | 2 cut(s) | 247, 358 |
| BmiI | GGNNCC | 1 cut(s) | 44 |
| BmrFI | CCNGG | 3 cut(s) | 117, 135, 158 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| BpuMI | CCSGG | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 1 cut(s) | 133 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 116 |
| Bse118I | RCCGGY | 2 cut(s) | 340, 360 |
| Bse1I | ACTGG | 1 cut(s) | 192 |
| BseBI | CCWGG | 2 cut(s) | 135, 158 |
| BseDI | CCNNGG | 1 cut(s) | 133 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 116 |
| BseNI | ACTGG | 1 cut(s) | 192 |
| BseXI | GCAGC | 2 cut(s) | 150, 153 |
| BseYI | CCCAGC | 2 cut(s) | 57, 319 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| Bsh1285I | CGRYCG | 3 cut(s) | 149, 316, 365 |
| BshFI | GGCC | 4 cut(s) | 156, 248, 308, 360 |
| BsiEI | CGRYCG | 3 cut(s) | 149, 316, 365 |
| BsiSI | CCGG | 3 cut(s) | 117, 341, 361 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 116 |
| BsnI | GGCC | 4 cut(s) | 156, 248, 308, 360 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspACI | CCGC | 9 cut(s) | 15, 38, 48, 72, 88, 107, 191, 316, 382 |
| BspANI | GGCC | 4 cut(s) | 156, 248, 308, 360 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 1 cut(s) | 44 |
| BspPI | GGATC | 2 cut(s) | 168, 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrFI | RCCGGY | 2 cut(s) | 340, 360 |
| BsrI | ACTGG | 1 cut(s) | 192 |
| BssAI | RCCGGY | 2 cut(s) | 340, 360 |
| BssECI | CCNNGG | 1 cut(s) | 133 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| Bst2UI | CCWGG | 2 cut(s) | 135, 158 |
| Bst4CI | ACNGT | 1 cut(s) | 150 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 332, 342 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstHHI | GCGC | 2 cut(s) | 92, 231 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMCI | CGRYCG | 3 cut(s) | 149, 316, 365 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 40, 87, 381 |
| BstNI | CCWGG | 2 cut(s) | 135, 158 |
| BstNSI | RCATGY | 1 cut(s) | 379 |
| BstSCI | CCNGG | 3 cut(s) | 115, 133, 156 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 2 cut(s) | 150, 153 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 4 cut(s) | 156, 248, 308, 360 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 332, 342 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 2 cut(s) | 92, 231 |
| Cfr10I | RCCGGY | 2 cut(s) | 340, 360 |
| Cfr13I | GGNCC | 2 cut(s) | 247, 358 |
| CseI | GACGC | 1 cut(s) | 307 |
| Csp6I | GTAC | 3 cut(s) | 175, 367, 379 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 100, 135 |
| CviAII | CATG | 3 cut(s) | 172, 271, 376 |
| CviJI | RGCY | 8 cut(s) | 43, 61, 144, 156, 248, 263, 308, 360 |
| CviKI_1 | RGCY | 8 cut(s) | 43, 61, 144, 156, 248, 263, 308, 360 |
| CviQI | GTAC | 3 cut(s) | 175, 367, 379 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| EcoRII | CCWGG | 2 cut(s) | 133, 156 |
| EcoT22I | ATGCAT | 1 cut(s) | 377 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| FaeI | CATG | 3 cut(s) | 175, 274, 379 |
| FaiI | YATR | 4 cut(s) | 173, 201, 272, 377 |
| FatI | CATG | 3 cut(s) | 171, 270, 375 |
| FauI | CCCGC | 5 cut(s) | 8, 31, 55, 79, 114 |
| Fnu4HI | GCNGC | 2 cut(s) | 139, 142 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 2 cut(s) | 139, 142 |
| FspBI | CTAG | 1 cut(s) | 302 |
| GlaI | GCGC | 2 cut(s) | 91, 230 |
| GluI | GCNGC | 2 cut(s) | 139, 142 |
| GsaI | CCCAGC | 2 cut(s) | 61, 323 |
| HaeIII | GGCC | 4 cut(s) | 156, 248, 308, 360 |
| HapII | CCGG | 3 cut(s) | 117, 341, 361 |
| HgaI | GACGC | 1 cut(s) | 307 |
| HhaI | GCGC | 2 cut(s) | 92, 231 |
| Hin1II | CATG | 3 cut(s) | 175, 274, 379 |
| Hin6I | GCGC | 2 cut(s) | 90, 229 |
| HinP1I | GCGC | 2 cut(s) | 90, 229 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 3 cut(s) | 117, 341, 361 |
| HphI | GGTGA | 2 cut(s) | 134, 160 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyAV | CCTTC | 1 cut(s) | 217 |
| HpyCH4III | ACNGT | 1 cut(s) | 150 |
| HpyCH4IV | ACGT | 2 cut(s) | 217, 277 |
| HpyCH4V | TGCA | 1 cut(s) | 375 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 40, 87, 381 |
| HpySE526I | ACGT | 2 cut(s) | 217, 277 |
| Hsp92II | CATG | 3 cut(s) | 175, 274, 379 |
| HspAI | GCGC | 2 cut(s) | 90, 229 |
| KroI | GCCGGC | 1 cut(s) | 340 |
| KroNI | GCCGGC | 1 cut(s) | 342 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 48, 226 |
| Lsp1109I | GCAGC | 2 cut(s) | 150, 153 |
| MaeI | CTAG | 1 cut(s) | 302 |
| MaeII | ACGT | 2 cut(s) | 217, 277 |
| MaeIII | GTNAC | 1 cut(s) | 166 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 307 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 5 cut(s) | 73, 118, 238, 277, 298 |
| Mph1103I | ATGCAT | 1 cut(s) | 377 |
| MroNI | GCCGGC | 1 cut(s) | 340 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 349 |
| MspA1I | CMGCKG | 2 cut(s) | 15, 384 |
| MspI | CCGG | 3 cut(s) | 117, 341, 361 |
| MspR9I | CCNGG | 3 cut(s) | 117, 135, 158 |
| MvaI | CCWGG | 2 cut(s) | 135, 158 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 40, 87, 381 |
| NaeI | GCCGGC | 1 cut(s) | 342 |
| NciI | CCSGG | 1 cut(s) | 117 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| NgoMIV | GCCGGC | 1 cut(s) | 340 |
| NlaIII | CATG | 3 cut(s) | 175, 274, 379 |
| NlaIV | GGNNCC | 1 cut(s) | 44 |
| NmuCI | GTSAC | 1 cut(s) | 166 |
| NsiI | ATGCAT | 1 cut(s) | 377 |
| NspI | RCATGY | 1 cut(s) | 379 |
| OliI | CACNNNNGTG | 1 cut(s) | 349 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 1 cut(s) | 342 |
| PkrI | GCNGC | 2 cut(s) | 140, 143 |
| Psp6I | CCWGG | 2 cut(s) | 133, 156 |
| PspFI | CCCAGC | 2 cut(s) | 57, 319 |
| PspGI | CCWGG | 2 cut(s) | 133, 156 |
| PspN4I | GGNNCC | 1 cut(s) | 44 |
| PspPI | GGNCC | 2 cut(s) | 247, 358 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 3 cut(s) | 176, 368, 380 |
| RsaNI | GTAC | 3 cut(s) | 175, 367, 379 |
| RseI | CAYNNNNRTG | 1 cut(s) | 349 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 2 cut(s) | 139, 142 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 2 cut(s) | 247, 358 |
| ScrFI | CCNGG | 3 cut(s) | 117, 135, 158 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SetI | ASST | 3 cut(s) | 220, 243, 280 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 349 |
| SsiI | CCGC | 9 cut(s) | 15, 38, 48, 72, 88, 107, 191, 316, 382 |
| SspMI | CTAG | 1 cut(s) | 302 |
| StyD4I | CCNGG | 3 cut(s) | 115, 133, 156 |
| TaaI | ACNGT | 1 cut(s) | 150 |
| TaiI | ACGT | 2 cut(s) | 220, 280 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 1 cut(s) | 166 |
| TseI | GCWGC | 2 cut(s) | 138, 141 |
| Tsp45I | GTSAC | 1 cut(s) | 166 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 192 |
| XceI | RCATGY | 1 cut(s) | 379 |
| XspI | CTAG | 1 cut(s) | 302 |
| Zsp2I | ATGCAT | 1 cut(s) | 377 |