Belongs to the RNase T2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 683 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 481 |
| AccII | CGCG | 3 cut(s) | 106, 544, 618 |
| AccIII | TCCGGA | 1 cut(s) | 249 |
| AclWI | GGATC | 2 cut(s) | 241, 386 |
| AcyI | GRCGYC | 1 cut(s) | 128 |
| AdeI | CACNNNGTG | 2 cut(s) | 459, 663 |
| AfaI | GTAC | 3 cut(s) | 5, 342, 590 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 214, 304, 481 |
| AgeI | ACCGGT | 1 cut(s) | 16 |
| AgsI | TTSAA | 2 cut(s) | 121, 418 |
| AleI | CACNNNNGTG | 1 cut(s) | 318 |
| AluBI | AGCT | 3 cut(s) | 88, 137, 501 |
| AluI | AGCT | 3 cut(s) | 88, 137, 501 |
| AlwI | GGATC | 2 cut(s) | 241, 386 |
| Ama87I | CYCGRG | 2 cut(s) | 265, 303 |
| Aor13HI | TCCGGA | 1 cut(s) | 249 |
| AoxI | GGCC | 5 cut(s) | 69, 75, 268, 306, 648 |
| ApaI | GGGCCC | 1 cut(s) | 310 |
| ApeKI | GCWGC | 1 cut(s) | 673 |
| AsiGI | ACCGGT | 1 cut(s) | 16 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 650 |
| AspLEI | GCGC | 2 cut(s) | 264, 546 |
| AspS9I | GGNCC | 5 cut(s) | 76, 178, 268, 306, 307 |
| AsuC2I | CCSGG | 3 cut(s) | 132, 266, 267 |
| AsuHPI | GGTGA | 5 cut(s) | 160, 305, 418, 500, 633 |
| AvaI | CYCGRG | 2 cut(s) | 265, 303 |
| AvaII | GGWCC | 1 cut(s) | 178 |
| BaeGI | GKGCMC | 1 cut(s) | 310 |
| BanI | GGYRCC | 1 cut(s) | 683 |
| BanII | GRGCYC | 1 cut(s) | 310 |
| BbvI | GCAGC | 1 cut(s) | 685 |
| BceAI | ACGGC | 4 cut(s) | 303, 505, 571, 671 |
| BcnI | CCSGG | 3 cut(s) | 132, 266, 267 |
| BfaI | CTAG | 2 cut(s) | 233, 694 |
| BfoI | RGCGCY | 1 cut(s) | 265 |
| BisI | GCNGC | 5 cut(s) | 153, 271, 668, 671, 674 |
| BlsI | GCNGC | 5 cut(s) | 154, 272, 669, 672, 675 |
| Bme1390I | CCNGG | 3 cut(s) | 132, 266, 267 |
| Bme18I | GGWCC | 1 cut(s) | 178 |
| BmeT110I | CYCGRG | 2 cut(s) | 265, 303 |
| BmgT120I | GGNCC | 5 cut(s) | 76, 178, 268, 306, 307 |
| BmiI | GGNNCC | 2 cut(s) | 308, 685 |
| BmrFI | CCNGG | 3 cut(s) | 132, 266, 267 |
| BmsI | GCATC | 4 cut(s) | 188, 213, 439, 685 |
| BpuMI | CCSGG | 3 cut(s) | 132, 266, 267 |
| BsaHI | GRCGYC | 1 cut(s) | 128 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 265 |
| BsaWI | WCCGGW | 2 cut(s) | 16, 249 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 214, 304, 481 |
| Bse118I | RCCGGY | 2 cut(s) | 16, 192 |
| BseAI | TCCGGA | 1 cut(s) | 249 |
| BseDI | CCNNGG | 2 cut(s) | 72, 265 |
| BseGI | GGATG | 2 cut(s) | 228, 572 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 214, 304, 481 |
| BseRI | GAGGAG | 2 cut(s) | 65, 268 |
| BseSI | GKGCMC | 1 cut(s) | 310 |
| BseXI | GCAGC | 1 cut(s) | 685 |
| Bsh1236I | CGCG | 3 cut(s) | 106, 544, 618 |
| BshFI | GGCC | 5 cut(s) | 71, 77, 270, 308, 650 |
| BshNI | GGYRCC | 1 cut(s) | 683 |
| BshTI | ACCGGT | 1 cut(s) | 16 |
| BsiHKCI | CYCGRG | 2 cut(s) | 265, 303 |
| BsiSI | CCGG | 6 cut(s) | 17, 131, 181, 193, 250, 266 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 214, 304, 481 |
| BsnI | GGCC | 5 cut(s) | 71, 77, 270, 308, 650 |
| BsoBI | CYCGRG | 2 cut(s) | 265, 303 |
| Bsp120I | GGGCCC | 1 cut(s) | 306 |
| Bsp1286I | GDGCHC | 1 cut(s) | 310 |
| Bsp13I | TCCGGA | 1 cut(s) | 249 |
| Bsp143I | GATC | 3 cut(s) | 185, 246, 391 |
| BspANI | GGCC | 5 cut(s) | 71, 77, 270, 308, 650 |
| BspEI | TCCGGA | 1 cut(s) | 249 |
| BspFNI | CGCG | 3 cut(s) | 106, 544, 618 |
| BspLI | GGNNCC | 2 cut(s) | 308, 685 |
| BspPI | GGATC | 2 cut(s) | 241, 386 |
| BspT107I | GGYRCC | 1 cut(s) | 683 |
| BsrFI | RCCGGY | 2 cut(s) | 16, 192 |
| BssAI | RCCGGY | 2 cut(s) | 16, 192 |
| BssECI | CCNNGG | 2 cut(s) | 72, 265 |
| BssMI | GATC | 3 cut(s) | 185, 246, 391 |
| BssNI | GRCGYC | 1 cut(s) | 128 |
| Bst4CI | ACNGT | 3 cut(s) | 345, 488, 683 |
| Bst6I | CTCTTC | 1 cut(s) | 116 |
| BstACI | GRCGYC | 1 cut(s) | 128 |
| BstC8I | GCNNGC | 2 cut(s) | 90, 194 |
| BstF5I | GGATG | 2 cut(s) | 228, 572 |
| BstFNI | CGCG | 3 cut(s) | 106, 544, 618 |
| BstH2I | RGCGCY | 1 cut(s) | 265 |
| BstHHI | GCGC | 2 cut(s) | 264, 546 |
| BstKTI | GATC | 3 cut(s) | 188, 249, 394 |
| BstMBI | GATC | 3 cut(s) | 185, 246, 391 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 94, 103, 149, 198, 270, 673 |
| BstSCI | CCNGG | 3 cut(s) | 130, 264, 265 |
| BstSLI | GKGCMC | 1 cut(s) | 310 |
| BstUI | CGCG | 3 cut(s) | 106, 544, 618 |
| BstV1I | GCAGC | 1 cut(s) | 685 |
| BsuRI | GGCC | 5 cut(s) | 71, 77, 270, 308, 650 |
| BtgZI | GCGATG | 2 cut(s) | 211, 509 |
| BtsCI | GGATG | 2 cut(s) | 228, 572 |
| Cac8I | GCNNGC | 2 cut(s) | 90, 194 |
| CfoI | GCGC | 2 cut(s) | 264, 546 |
| Cfr10I | RCCGGY | 2 cut(s) | 16, 192 |
| Cfr13I | GGNCC | 5 cut(s) | 76, 178, 268, 306, 307 |
| Cfr9I | CCCGGG | 1 cut(s) | 265 |
| CseI | GACGC | 2 cut(s) | 136, 607 |
| Csp6I | GTAC | 3 cut(s) | 4, 341, 589 |
| CspAI | ACCGGT | 1 cut(s) | 16 |
| CviAII | CATG | 1 cut(s) | 406 |
| CviQI | GTAC | 3 cut(s) | 4, 341, 589 |
| DpnI | GATC | 3 cut(s) | 187, 248, 393 |
| DpnII | GATC | 3 cut(s) | 185, 246, 391 |
| DraIII | CACNNNGTG | 2 cut(s) | 459, 663 |
| Eam1104I | CTCTTC | 1 cut(s) | 116 |
| EarI | CTCTTC | 1 cut(s) | 116 |
| EciI | GGCGGA | 3 cut(s) | 396, 507, 573 |
| Eco147I | AGGCCT | 1 cut(s) | 650 |
| Eco24I | GRGCYC | 1 cut(s) | 310 |
| Eco47I | GGWCC | 1 cut(s) | 178 |
| Eco88I | CYCGRG | 2 cut(s) | 265, 303 |
| EcoO109I | RGGNCCY | 1 cut(s) | 307 |
| EcoT38I | GRGCYC | 1 cut(s) | 310 |
| FaeI | CATG | 1 cut(s) | 409 |
| FaiI | YATR | 4 cut(s) | 30, 351, 378, 407 |
| FatI | CATG | 1 cut(s) | 405 |
| Fnu4HI | GCNGC | 5 cut(s) | 153, 271, 668, 671, 674 |
| FokI | GGATG | 2 cut(s) | 235, 559 |
| FriOI | GRGCYC | 1 cut(s) | 310 |
| Fsp4HI | GCNGC | 5 cut(s) | 153, 271, 668, 671, 674 |
| FspBI | CTAG | 2 cut(s) | 233, 694 |
| GlaI | GCGC | 2 cut(s) | 263, 545 |
| GluI | GCNGC | 5 cut(s) | 153, 271, 668, 671, 674 |
| HaeII | RGCGCY | 1 cut(s) | 265 |
| HaeIII | GGCC | 5 cut(s) | 71, 77, 270, 308, 650 |
| HapII | CCGG | 6 cut(s) | 17, 131, 181, 193, 250, 266 |
| HgaI | GACGC | 2 cut(s) | 136, 607 |
| HhaI | GCGC | 2 cut(s) | 264, 546 |
| Hin1I | GRCGYC | 1 cut(s) | 128 |
| Hin1II | CATG | 1 cut(s) | 409 |
| Hin6I | GCGC | 2 cut(s) | 262, 544 |
| HinP1I | GCGC | 2 cut(s) | 262, 544 |
| HinfI | GANTC | 5 cut(s) | 256, 281, 323, 532, 638 |
| HpaII | CCGG | 6 cut(s) | 17, 131, 181, 193, 250, 266 |
| HphI | GGTGA | 5 cut(s) | 160, 305, 418, 500, 633 |
| Hpy166II | GTNNAC | 1 cut(s) | 403 |
| Hpy188I | TCNGA | 1 cut(s) | 391 |
| Hpy188III | TCNNGA | 1 cut(s) | 250 |
| Hpy8I | GTNNAC | 1 cut(s) | 403 |
| HpyAV | CCTTC | 6 cut(s) | 320, 406, 424, 567, 640, 661 |
| HpyCH4III | ACNGT | 3 cut(s) | 345, 488, 683 |
| HpyCH4IV | ACGT | 1 cut(s) | 46 |
| HpyCH4V | TGCA | 3 cut(s) | 201, 226, 353 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 94, 103, 149, 198, 270, 673 |
| HpySE526I | ACGT | 1 cut(s) | 46 |
| Hsp92I | GRCGYC | 1 cut(s) | 128 |
| Hsp92II | CATG | 1 cut(s) | 409 |
| HspAI | GCGC | 2 cut(s) | 262, 544 |
| Kpn2I | TCCGGA | 1 cut(s) | 249 |
| KroI | GCCGGC | 1 cut(s) | 192 |
| KroNI | GCCGGC | 1 cut(s) | 194 |
| Kzo9I | GATC | 3 cut(s) | 185, 246, 391 |
| LmnI | GCTCC | 2 cut(s) | 134, 629 |
| LpnPI | CCDG | 9 cut(s) | 30, 74, 144, 162, 194, 206, 263, 279, 487 |
| Lsp1109I | GCAGC | 1 cut(s) | 685 |
| LweI | GCATC | 4 cut(s) | 188, 213, 439, 685 |
| MaeI | CTAG | 2 cut(s) | 233, 694 |
| MaeII | ACGT | 1 cut(s) | 46 |
| MaeIII | GTNAC | 3 cut(s) | 424, 655, 688 |
| MalI | GATC | 3 cut(s) | 187, 248, 393 |
| MboI | GATC | 3 cut(s) | 185, 246, 391 |
| MboII | GAAGA | 1 cut(s) | 133 |
| MhlI | GDGCHC | 1 cut(s) | 310 |
| MluCI | AATT | 1 cut(s) | 116 |
| MlyI | GAGTC | 2 cut(s) | 265, 526 |
| MnlI | CCTC | 7 cut(s) | 43, 46, 67, 117, 234, 246, 484 |
| MreI | CGCCGGCG | 1 cut(s) | 192 |
| MroI | TCCGGA | 1 cut(s) | 249 |
| MroNI | GCCGGC | 1 cut(s) | 192 |
| MroXI | GAANNNNTTC | 1 cut(s) | 650 |
| MslI | CAYNNNNRTG | 3 cut(s) | 173, 318, 681 |
| MspA1I | CMGCKG | 1 cut(s) | 88 |
| MspI | CCGG | 6 cut(s) | 17, 131, 181, 193, 250, 266 |
| MspR9I | CCNGG | 3 cut(s) | 132, 266, 267 |
| MvnI | CGCG | 3 cut(s) | 106, 544, 618 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 94, 103, 149, 198, 270, 673 |
| NaeI | GCCGGC | 1 cut(s) | 194 |
| NciI | CCSGG | 3 cut(s) | 132, 266, 267 |
| NdeII | GATC | 3 cut(s) | 185, 246, 391 |
| NgoMIV | GCCGGC | 1 cut(s) | 192 |
| NlaIII | CATG | 1 cut(s) | 409 |
| NlaIV | GGNNCC | 2 cut(s) | 308, 685 |
| NmeAIII | GCCGAG | 1 cut(s) | 97 |
| NmuCI | GTSAC | 3 cut(s) | 424, 655, 688 |
| OliI | CACNNNNGTG | 1 cut(s) | 318 |
| PceI | AGGCCT | 1 cut(s) | 650 |
| PdiI | GCCGGC | 1 cut(s) | 194 |
| PdmI | GAANNNNTTC | 1 cut(s) | 650 |
| PfeI | GAWTC | 3 cut(s) | 281, 323, 638 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 481 |
| PinAI | ACCGGT | 1 cut(s) | 16 |
| PkrI | GCNGC | 5 cut(s) | 154, 272, 669, 672, 675 |
| PleI | GAGTC | 2 cut(s) | 264, 526 |
| PpsI | GAGTC | 2 cut(s) | 264, 526 |
| PspN4I | GGNNCC | 2 cut(s) | 308, 685 |
| PspOMI | GGGCCC | 1 cut(s) | 306 |
| PspPI | GGNCC | 5 cut(s) | 76, 178, 268, 306, 307 |
| PvuII | CAGCTG | 1 cut(s) | 88 |
| RsaI | GTAC | 3 cut(s) | 5, 342, 590 |
| RsaNI | GTAC | 3 cut(s) | 4, 341, 589 |
| RseI | CAYNNNNRTG | 3 cut(s) | 173, 318, 681 |
| SatI | GCNGC | 5 cut(s) | 153, 271, 668, 671, 674 |
| Sau3AI | GATC | 3 cut(s) | 185, 246, 391 |
| Sau96I | GGNCC | 5 cut(s) | 76, 178, 268, 306, 307 |
| SchI | GAGTC | 2 cut(s) | 265, 526 |
| ScrFI | CCNGG | 3 cut(s) | 132, 266, 267 |
| SduI | GDGCHC | 1 cut(s) | 310 |
| SetI | ASST | 8 cut(s) | 49, 90, 139, 245, 416, 503, 665, 695 |
| SfaNI | GCATC | 4 cut(s) | 188, 213, 439, 685 |
| SgrAI | CRCCGGYG | 2 cut(s) | 16, 192 |
| SinI | GGWCC | 1 cut(s) | 178 |
| SmaI | CCCGGG | 1 cut(s) | 267 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 173, 318, 681 |
| SrfI | GCCCGGGC | 1 cut(s) | 267 |
| Sse9I | AATT | 1 cut(s) | 116 |
| SseBI | AGGCCT | 1 cut(s) | 650 |
| SspMI | CTAG | 2 cut(s) | 233, 694 |
| StuI | AGGCCT | 1 cut(s) | 650 |
| StyD4I | CCNGG | 3 cut(s) | 130, 264, 265 |
| TaaI | ACNGT | 3 cut(s) | 345, 488, 683 |
| TaiI | ACGT | 1 cut(s) | 49 |
| TaqI | TCGA | 1 cut(s) | 279 |
| TasI | AATT | 1 cut(s) | 116 |
| TauI | GCSGC | 4 cut(s) | 155, 273, 670, 673 |
| TfiI | GAWTC | 3 cut(s) | 281, 323, 638 |
| TseFI | GTSAC | 3 cut(s) | 424, 655, 688 |
| TseI | GCWGC | 1 cut(s) | 673 |
| Tsp45I | GTSAC | 3 cut(s) | 424, 655, 688 |
| TspDTI | ATGAA | 4 cut(s) | 17, 129, 561, 651 |
| TspMI | CCCGGG | 1 cut(s) | 265 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 481 |
| VpaK11BI | GGWCC | 1 cut(s) | 178 |
| XmaI | CCCGGG | 1 cut(s) | 265 |
| XmnI | GAANNNNTTC | 1 cut(s) | 650 |
| XspI | CTAG | 2 cut(s) | 233, 694 |