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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 394 |
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 406 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccII | CGCG | 1 cut(s) | 90 |
| AcuI | CTGAAG | 2 cut(s) | 48, 546 |
| AfaI | GTAC | 3 cut(s) | 176, 381, 408 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 202, 368 |
| AgsI | TTSAA | 1 cut(s) | 100 |
| AjnI | CCWGG | 2 cut(s) | 347, 382 |
| AluBI | AGCT | 2 cut(s) | 181, 512 |
| AluI | AGCT | 2 cut(s) | 181, 512 |
| AoxI | GGCC | 4 cut(s) | 154, 240, 246, 359 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 342 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 4 cut(s) | 92, 187, 231, 552 |
| AspS9I | GGNCC | 9 cut(s) | 40, 154, 193, 247, 360, 386, 415, 440, 559 |
| AsuC2I | CCSGG | 1 cut(s) | 117 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| AsuNHI | GCTAGC | 1 cut(s) | 187 |
| AvaII | GGWCC | 6 cut(s) | 40, 193, 386, 415, 440, 559 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 363, 396 |
| BanI | GGYRCC | 2 cut(s) | 379, 406 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 354 |
| BccI | CCATC | 3 cut(s) | 59, 430, 458 |
| BciT130I | CCWGG | 2 cut(s) | 349, 384 |
| BcnI | CCSGG | 1 cut(s) | 117 |
| BfaI | CTAG | 3 cut(s) | 188, 513, 574 |
| BfoI | RGCGCY | 1 cut(s) | 188 |
| BfuAI | ACCTGC | 1 cut(s) | 394 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 5 cut(s) | 139, 142, 145, 343, 491 |
| BlsI | GCNGC | 5 cut(s) | 140, 143, 146, 344, 492 |
| Bme1390I | CCNGG | 3 cut(s) | 117, 349, 384 |
| Bme18I | GGWCC | 6 cut(s) | 40, 193, 386, 415, 440, 559 |
| BmgT120I | GGNCC | 9 cut(s) | 40, 154, 193, 247, 360, 386, 415, 440, 559 |
| BmiI | GGNNCC | 3 cut(s) | 381, 408, 441 |
| BmrFI | CCNGG | 3 cut(s) | 117, 349, 384 |
| BmsI | GCATC | 1 cut(s) | 390 |
| BmtI | GCTAGC | 1 cut(s) | 191 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 3 cut(s) | 115, 243, 545 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 151, 202, 368 |
| Bse1I | ACTGG | 3 cut(s) | 43, 373, 389 |
| BseBI | CCWGG | 2 cut(s) | 349, 384 |
| BseDI | CCNNGG | 3 cut(s) | 115, 243, 545 |
| BseGI | GGATG | 3 cut(s) | 51, 441, 450 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 202, 368 |
| BseNI | ACTGG | 3 cut(s) | 43, 373, 389 |
| BseRI | GAGGAG | 1 cut(s) | 239 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 354 |
| BseYI | CCCAGC | 2 cut(s) | 70, 106 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BshNI | GGYRCC | 2 cut(s) | 379, 406 |
| BsiSI | CCGG | 1 cut(s) | 117 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 202, 368 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsnI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| Bsp143I | GATC | 1 cut(s) | 160 |
| BspANI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspLI | GGNNCC | 3 cut(s) | 381, 408, 441 |
| BspMI | ACCTGC | 1 cut(s) | 394 |
| BspOI | GCTAGC | 1 cut(s) | 191 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 406 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrI | ACTGG | 3 cut(s) | 43, 373, 389 |
| BssECI | CCNNGG | 3 cut(s) | 115, 243, 545 |
| BssMI | GATC | 1 cut(s) | 160 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 2 cut(s) | 349, 384 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 216 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 183, 189, 332 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 200 |
| BstF5I | GGATG | 3 cut(s) | 51, 441, 450 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 1 cut(s) | 188 |
| BstHHI | GCGC | 4 cut(s) | 92, 187, 231, 552 |
| BstKTI | GATC | 1 cut(s) | 163 |
| BstMBI | GATC | 1 cut(s) | 160 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 144, 153, 320, 358, 409 |
| BstNI | CCWGG | 2 cut(s) | 349, 384 |
| BstSCI | CCNGG | 3 cut(s) | 115, 347, 382 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 354 |
| BstX2I | RGATCY | 1 cut(s) | 160 |
| BstYI | RGATCY | 1 cut(s) | 160 |
| BsuRI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BtsCI | GGATG | 3 cut(s) | 51, 441, 450 |
| BtsIMutI | CAGTG | 1 cut(s) | 366 |
| BveI | ACCTGC | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 183, 189, 332 |
| CfoI | GCGC | 4 cut(s) | 92, 187, 231, 552 |
| Cfr13I | GGNCC | 9 cut(s) | 40, 154, 193, 247, 360, 386, 415, 440, 559 |
| CseI | GACGC | 1 cut(s) | 307 |
| Csp6I | GTAC | 3 cut(s) | 175, 380, 407 |
| CviJI | RGCY | 9 cut(s) | 110, 144, 156, 181, 242, 248, 263, 361, 512 |
| CviKI_1 | RGCY | 9 cut(s) | 110, 144, 156, 181, 242, 248, 263, 361, 512 |
| CviQI | GTAC | 3 cut(s) | 175, 380, 407 |
| DpnI | GATC | 1 cut(s) | 162 |
| DpnII | GATC | 1 cut(s) | 160 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 216 |
| EarI | CTCTTC | 2 cut(s) | 24, 216 |
| EciI | GGCGGA | 3 cut(s) | 37, 53, 525 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco47I | GGWCC | 6 cut(s) | 40, 193, 386, 415, 440, 559 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 546 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 200 |
| EcoRII | CCWGG | 2 cut(s) | 347, 382 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| FaiI | YATR | 2 cut(s) | 173, 395 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FauI | CCCGC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 5 cut(s) | 139, 142, 145, 343, 491 |
| FokI | GGATG | 3 cut(s) | 38, 437, 448 |
| Fsp4HI | GCNGC | 5 cut(s) | 139, 142, 145, 343, 491 |
| FspBI | CTAG | 3 cut(s) | 188, 513, 574 |
| GlaI | GCGC | 4 cut(s) | 91, 186, 230, 551 |
| GluI | GCNGC | 5 cut(s) | 139, 142, 145, 343, 491 |
| GsaI | CCCAGC | 2 cut(s) | 74, 110 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 188 |
| HaeIII | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| HapII | CCGG | 1 cut(s) | 117 |
| HgaI | GACGC | 1 cut(s) | 307 |
| HhaI | GCGC | 4 cut(s) | 92, 187, 231, 552 |
| Hin6I | GCGC | 4 cut(s) | 90, 185, 229, 550 |
| HinP1I | GCGC | 4 cut(s) | 90, 185, 229, 550 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 1 cut(s) | 117 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyAV | CCTTC | 1 cut(s) | 521 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 277 |
| HpyCH4V | TGCA | 1 cut(s) | 403 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 144, 153, 320, 358, 409 |
| HpySE526I | ACGT | 1 cut(s) | 277 |
| HspAI | GCGC | 4 cut(s) | 90, 185, 229, 550 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 1 cut(s) | 160 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 226, 469 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 354 |
| LweI | GCATC | 1 cut(s) | 390 |
| MaeI | CTAG | 3 cut(s) | 188, 513, 574 |
| MaeII | ACGT | 1 cut(s) | 277 |
| MaeIII | GTNAC | 1 cut(s) | 273 |
| MalI | GATC | 1 cut(s) | 162 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboI | GATC | 1 cut(s) | 160 |
| MboII | GAAGA | 2 cut(s) | 41, 233 |
| MflI | RGATCY | 1 cut(s) | 160 |
| MmeI | TCCRAC | 2 cut(s) | 90, 447 |
| MnlI | CCTC | 7 cut(s) | 73, 118, 206, 217, 238, 469, 513 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 398 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 412, 565 |
| MspI | CCGG | 1 cut(s) | 117 |
| MspR9I | CCNGG | 3 cut(s) | 117, 349, 384 |
| MvaI | CCWGG | 2 cut(s) | 349, 384 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 144, 153, 320, 358, 409 |
| NciI | CCSGG | 1 cut(s) | 117 |
| NdeII | GATC | 1 cut(s) | 160 |
| NheI | GCTAGC | 1 cut(s) | 187 |
| NlaIV | GGNNCC | 3 cut(s) | 381, 408, 441 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 273 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 482 |
| PkrI | GCNGC | 5 cut(s) | 140, 143, 146, 344, 492 |
| Psp6I | CCWGG | 2 cut(s) | 347, 382 |
| PspFI | CCCAGC | 2 cut(s) | 70, 106 |
| PspGI | CCWGG | 2 cut(s) | 347, 382 |
| PspN4I | GGNNCC | 3 cut(s) | 381, 408, 441 |
| PspPI | GGNCC | 9 cut(s) | 40, 154, 193, 247, 360, 386, 415, 440, 559 |
| PsuI | RGATCY | 1 cut(s) | 160 |
| RsaI | GTAC | 3 cut(s) | 176, 381, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 380, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 398 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 5 cut(s) | 139, 142, 145, 343, 491 |
| Sau3AI | GATC | 1 cut(s) | 160 |
| Sau96I | GGNCC | 9 cut(s) | 40, 154, 193, 247, 360, 386, 415, 440, 559 |
| ScrFI | CCNGG | 3 cut(s) | 117, 349, 384 |
| SetI | ASST | 9 cut(s) | 82, 183, 198, 280, 408, 420, 424, 514, 561 |
| SfaNI | GCATC | 1 cut(s) | 390 |
| SinI | GGWCC | 6 cut(s) | 40, 193, 386, 415, 440, 559 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 398 |
| SspMI | CTAG | 3 cut(s) | 188, 513, 574 |
| StyD4I | CCNGG | 3 cut(s) | 115, 347, 382 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 280 |
| TaqI | TCGA | 1 cut(s) | 506 |
| TauI | GCSGC | 2 cut(s) | 147, 493 |
| TfiI | GAWTC | 1 cut(s) | 482 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 373 |
| TseFI | GTSAC | 1 cut(s) | 273 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 342 |
| Tsp45I | GTSAC | 1 cut(s) | 273 |
| TspDTI | ATGAA | 1 cut(s) | 300 |
| TspRI | CASTG | 1 cut(s) | 373 |
| VpaK11BI | GGWCC | 6 cut(s) | 40, 193, 386, 415, 440, 559 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 200 |
| XspI | CTAG | 3 cut(s) | 188, 513, 574 |