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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 727 |
| Acc36I | ACCTGC | 1 cut(s) | 727 |
| AccBSI | CCGCTC | 1 cut(s) | 444 |
| AccI | GTMKAC | 1 cut(s) | 838 |
| AccII | CGCG | 3 cut(s) | 259, 439, 820 |
| AclWI | GGATC | 4 cut(s) | 57, 554, 567, 648 |
| AcoI | YGGCCR | 2 cut(s) | 76, 741 |
| AcsI | RAATTY | 1 cut(s) | 758 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 85, 138, 556, 682, 736, 848, 916, 917, 1035 |
| AgeI | ACCGGT | 1 cut(s) | 848 |
| AjnI | CCWGG | 3 cut(s) | 564, 613, 631 |
| AluBI | AGCT | 8 cut(s) | 136, 157, 357, 464, 522, 602, 970, 1021 |
| AluI | AGCT | 8 cut(s) | 136, 157, 357, 464, 522, 602, 970, 1021 |
| Alw21I | GWGCWC | 1 cut(s) | 827 |
| Alw26I | GTCTC | 5 cut(s) | 897, 938, 955, 1015, 1073 |
| AlwI | GGATC | 4 cut(s) | 57, 554, 567, 648 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1013 |
| Ama87I | CYCGRG | 1 cut(s) | 27 |
| AoxI | GGCC | 4 cut(s) | 76, 606, 726, 741 |
| ApeKI | GCWGC | 9 cut(s) | 187, 209, 239, 294, 303, 332, 357, 366, 618 |
| ApoI | RAATTY | 1 cut(s) | 758 |
| AsiGI | ACCGGT | 1 cut(s) | 848 |
| AspLEI | GCGC | 5 cut(s) | 259, 321, 441, 781, 805 |
| AspS9I | GGNCC | 4 cut(s) | 481, 580, 673, 727 |
| AsuC2I | CCSGG | 2 cut(s) | 67, 412 |
| AsuHPI | GGTGA | 5 cut(s) | 121, 184, 211, 409, 420 |
| AsuNHI | GCTAGC | 3 cut(s) | 913, 970, 1031 |
| AvaI | CYCGRG | 1 cut(s) | 27 |
| AvaII | GGWCC | 3 cut(s) | 481, 580, 673 |
| BalI | TGGCCA | 1 cut(s) | 743 |
| BamHI | GGATCC | 1 cut(s) | 559 |
| BanII | GRGCYC | 1 cut(s) | 572 |
| BauI | CACGAG | 1 cut(s) | 374 |
| BbsI | GAAGAC | 1 cut(s) | 725 |
| Bbv12I | GWGCWC | 1 cut(s) | 827 |
| BbvCI | CCTCAGC | 1 cut(s) | 603 |
| BbvI | GCAGC | 9 cut(s) | 199, 221, 226, 306, 315, 344, 344, 378, 630 |
| BccI | CCATC | 2 cut(s) | 140, 163 |
| BciT130I | CCWGG | 3 cut(s) | 566, 615, 633 |
| BcnI | CCSGG | 2 cut(s) | 67, 412 |
| BcoDI | GTCTC | 5 cut(s) | 897, 938, 955, 1015, 1073 |
| BfaI | CTAG | 6 cut(s) | 38, 467, 552, 914, 971, 1032 |
| BfmI | CTRYAG | 4 cut(s) | 45, 240, 248, 597 |
| BfoI | RGCGCY | 1 cut(s) | 782 |
| BfuAI | ACCTGC | 1 cut(s) | 727 |
| BlpI | GCTNAGC | 2 cut(s) | 899, 1017 |
| Bme1390I | CCNGG | 5 cut(s) | 67, 412, 566, 615, 633 |
| Bme18I | GGWCC | 3 cut(s) | 481, 580, 673 |
| BmeT110I | CYCGRG | 1 cut(s) | 27 |
| BmgT120I | GGNCC | 4 cut(s) | 481, 580, 673, 727 |
| BmiI | GGNNCC | 2 cut(s) | 561, 582 |
| BmrFI | CCNGG | 5 cut(s) | 67, 412, 566, 615, 633 |
| BmsI | GCATC | 3 cut(s) | 251, 299, 464 |
| BmtI | GCTAGC | 3 cut(s) | 917, 974, 1035 |
| BoxI | GACNNNNGTC | 1 cut(s) | 486 |
| BpiI | GAAGAC | 1 cut(s) | 725 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 935, 967, 995, 1027 |
| BpmI | CTGGAG | 1 cut(s) | 803 |
| Bpu10I | CCTNAGC | 1 cut(s) | 603 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 899, 1017 |
| BpuEI | CTTGAG | 1 cut(s) | 416 |
| BpuMI | CCSGG | 2 cut(s) | 67, 412 |
| BsaJI | CCNNGG | 4 cut(s) | 410, 564, 565, 723 |
| BsaWI | WCCGGW | 1 cut(s) | 848 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 85, 138, 556, 682, 736, 848, 916, 917, 1035 |
| Bse118I | RCCGGY | 1 cut(s) | 848 |
| Bse1I | ACTGG | 1 cut(s) | 484 |
| Bse3DI | GCAATG | 1 cut(s) | 531 |
| BseBI | CCWGG | 3 cut(s) | 566, 615, 633 |
| BseDI | CCNNGG | 4 cut(s) | 410, 564, 565, 723 |
| BseGI | GGATG | 1 cut(s) | 24 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 85, 138, 556, 682, 736, 848, 916, 917, 1035 |
| BseMI | GCAATG | 1 cut(s) | 531 |
| BseMII | CTCAG | 4 cut(s) | 516, 590, 594, 980 |
| BseNI | ACTGG | 1 cut(s) | 484 |
| BseXI | GCAGC | 9 cut(s) | 199, 221, 226, 306, 315, 344, 344, 378, 630 |
| BseYI | CCCAGC | 3 cut(s) | 132, 388, 799 |
| BsgI | GTGCAG | 2 cut(s) | 228, 360 |
| Bsh1236I | CGCG | 3 cut(s) | 259, 439, 820 |
| BshFI | GGCC | 4 cut(s) | 78, 608, 728, 743 |
| BshTI | ACCGGT | 1 cut(s) | 848 |
| BsiHKAI | GWGCWC | 1 cut(s) | 827 |
| BsiHKCI | CYCGRG | 1 cut(s) | 27 |
| BsiSI | CCGG | 4 cut(s) | 66, 79, 412, 849 |
| BslFI | GGGAC | 5 cut(s) | 566, 875, 933, 990, 1051 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 85, 138, 556, 682, 736, 848, 916, 917, 1035 |
| BsmAI | GTCTC | 5 cut(s) | 897, 938, 955, 1015, 1073 |
| BsmFI | GGGAC | 5 cut(s) | 566, 875, 933, 990, 1051 |
| BsnI | GGCC | 4 cut(s) | 78, 608, 728, 743 |
| BsoBI | CYCGRG | 1 cut(s) | 27 |
| Bsp1286I | GDGCHC | 2 cut(s) | 572, 827 |
| Bsp143I | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 899, 1017 |
| BspANI | GGCC | 4 cut(s) | 78, 608, 728, 743 |
| BspCNI | CTCAG | 4 cut(s) | 517, 589, 595, 979 |
| BspFNI | CGCG | 3 cut(s) | 259, 439, 820 |
| BspLI | GGNNCC | 2 cut(s) | 561, 582 |
| BspMAI | CTGCAG | 2 cut(s) | 244, 252 |
| BspMI | ACCTGC | 1 cut(s) | 727 |
| BspOI | GCTAGC | 3 cut(s) | 917, 974, 1035 |
| BspPI | GGATC | 4 cut(s) | 57, 554, 567, 648 |
| BsrBI | CCGCTC | 1 cut(s) | 444 |
| BsrDI | GCAATG | 1 cut(s) | 531 |
| BsrFI | RCCGGY | 1 cut(s) | 848 |
| BsrI | ACTGG | 1 cut(s) | 484 |
| BssAI | RCCGGY | 1 cut(s) | 848 |
| BssECI | CCNNGG | 4 cut(s) | 410, 564, 565, 723 |
| BssMI | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| BssSI | CACGAG | 1 cut(s) | 374 |
| Bst2BI | CACGAG | 1 cut(s) | 374 |
| Bst2UI | CCWGG | 3 cut(s) | 566, 615, 633 |
| Bst4CI | ACNGT | 1 cut(s) | 949 |
| Bst6I | CTCTTC | 1 cut(s) | 170 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 309, 338, 1013 |
| BstDEI | CTNAG | 6 cut(s) | 525, 576, 603, 899, 966, 1017 |
| BstEII | GGTNACC | 1 cut(s) | 127 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 680 |
| BstF5I | GGATG | 1 cut(s) | 24 |
| BstFNI | CGCG | 3 cut(s) | 259, 439, 820 |
| BstH2I | RGCGCY | 1 cut(s) | 782 |
| BstHHI | GCGC | 5 cut(s) | 259, 321, 441, 781, 805 |
| BstKTI | GATC | 5 cut(s) | 65, 428, 562, 643, 661 |
| BstMAI | GTCTC | 5 cut(s) | 897, 938, 955, 1015, 1073 |
| BstMBI | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| BstNI | CCWGG | 3 cut(s) | 566, 615, 633 |
| BstNSI | RCATGY | 2 cut(s) | 36, 373 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 486 |
| BstPI | GGTNACC | 1 cut(s) | 127 |
| BstSCI | CCNGG | 5 cut(s) | 65, 410, 564, 613, 631 |
| BstSFI | CTRYAG | 4 cut(s) | 45, 240, 248, 597 |
| BstUI | CGCG | 3 cut(s) | 259, 439, 820 |
| BstV1I | GCAGC | 9 cut(s) | 199, 221, 226, 306, 315, 344, 344, 378, 630 |
| BstV2I | GAAGAC | 1 cut(s) | 725 |
| BstX2I | RGATCY | 3 cut(s) | 62, 559, 640 |
| BstYI | RGATCY | 3 cut(s) | 62, 559, 640 |
| BsuRI | GGCC | 4 cut(s) | 78, 608, 728, 743 |
| BtgZI | GCGATG | 1 cut(s) | 274 |
| BtsCI | GGATG | 1 cut(s) | 24 |
| BtsIMutI | CAGTG | 1 cut(s) | 491 |
| BveI | ACCTGC | 1 cut(s) | 727 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1013 |
| CfoI | GCGC | 5 cut(s) | 259, 321, 441, 781, 805 |
| Cfr10I | RCCGGY | 1 cut(s) | 848 |
| Cfr13I | GGNCC | 4 cut(s) | 481, 580, 673, 727 |
| CseI | GACGC | 2 cut(s) | 445, 532 |
| CsiI | ACCWGGT | 1 cut(s) | 631 |
| CspAI | ACCGGT | 1 cut(s) | 848 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 832, 867 |
| CviAII | CATG | 2 cut(s) | 33, 370 |
| DdeI | CTNAG | 6 cut(s) | 525, 576, 603, 899, 966, 1017 |
| DpnI | GATC | 5 cut(s) | 64, 427, 561, 642, 660 |
| DpnII | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| EaeI | YGGCCR | 2 cut(s) | 76, 741 |
| Eam1104I | CTCTTC | 1 cut(s) | 170 |
| EarI | CTCTTC | 1 cut(s) | 170 |
| Eco24I | GRGCYC | 1 cut(s) | 572 |
| Eco47I | GGWCC | 3 cut(s) | 481, 580, 673 |
| Eco88I | CYCGRG | 1 cut(s) | 27 |
| Eco91I | GGTNACC | 1 cut(s) | 127 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 680 |
| EcoO109I | RGGNCCY | 1 cut(s) | 580 |
| EcoO65I | GGTNACC | 1 cut(s) | 127 |
| EcoRII | CCWGG | 3 cut(s) | 564, 613, 631 |
| EcoT38I | GRGCYC | 1 cut(s) | 572 |
| FaeI | CATG | 2 cut(s) | 36, 373 |
| FaiI | YATR | 7 cut(s) | 34, 86, 139, 371, 653, 657, 681 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 167, 199 |
| FaqI | GGGAC | 5 cut(s) | 566, 875, 933, 990, 1051 |
| FatI | CATG | 2 cut(s) | 32, 369 |
| FauI | CCCGC | 8 cut(s) | 290, 353, 591, 852, 910, 918, 967, 1028 |
| FblI | GTMKAC | 1 cut(s) | 838 |
| FokI | GGATG | 1 cut(s) | 31 |
| FriOI | GRGCYC | 1 cut(s) | 572 |
| FspBI | CTAG | 6 cut(s) | 38, 467, 552, 914, 971, 1032 |
| GlaI | GCGC | 5 cut(s) | 258, 320, 440, 780, 804 |
| GsaI | CCCAGC | 3 cut(s) | 136, 392, 803 |
| GsuI | CTGGAG | 1 cut(s) | 803 |
| HaeII | RGCGCY | 1 cut(s) | 782 |
| HaeIII | GGCC | 4 cut(s) | 78, 608, 728, 743 |
| HapII | CCGG | 4 cut(s) | 66, 79, 412, 849 |
| HgaI | GACGC | 2 cut(s) | 445, 532 |
| HhaI | GCGC | 5 cut(s) | 259, 321, 441, 781, 805 |
| Hin1II | CATG | 2 cut(s) | 36, 373 |
| Hin6I | GCGC | 5 cut(s) | 257, 319, 439, 779, 803 |
| HinP1I | GCGC | 5 cut(s) | 257, 319, 439, 779, 803 |
| HincII | GTYRAC | 1 cut(s) | 547 |
| HindII | GTYRAC | 1 cut(s) | 547 |
| HinfI | GANTC | 3 cut(s) | 863, 921, 1039 |
| HpaII | CCGG | 4 cut(s) | 66, 79, 412, 849 |
| HphI | GGTGA | 5 cut(s) | 121, 184, 211, 409, 420 |
| Hpy166II | GTNNAC | 3 cut(s) | 547, 701, 839 |
| Hpy188I | TCNGA | 1 cut(s) | 526 |
| Hpy188III | TCNNGA | 3 cut(s) | 16, 467, 638 |
| Hpy8I | GTNNAC | 3 cut(s) | 547, 701, 839 |
| Hpy99I | CGWCG | 2 cut(s) | 127, 767 |
| HpyAV | CCTTC | 2 cut(s) | 878, 1054 |
| HpyCH4III | ACNGT | 1 cut(s) | 949 |
| HpyCH4IV | ACGT | 2 cut(s) | 122, 832 |
| HpyF3I | CTNAG | 6 cut(s) | 525, 576, 603, 899, 966, 1017 |
| HpySE526I | ACGT | 2 cut(s) | 122, 832 |
| Hsp92II | CATG | 2 cut(s) | 36, 373 |
| HspAI | GCGC | 5 cut(s) | 257, 319, 439, 779, 803 |
| Kzo9I | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| LmnI | GCTCC | 6 cut(s) | 149, 316, 329, 354, 449, 822 |
| Lsp1109I | GCAGC | 9 cut(s) | 199, 221, 226, 306, 315, 344, 344, 378, 630 |
| LweI | GCATC | 3 cut(s) | 251, 299, 464 |
| MabI | ACCWGGT | 1 cut(s) | 631 |
| MaeI | CTAG | 6 cut(s) | 38, 467, 552, 914, 971, 1032 |
| MaeII | ACGT | 2 cut(s) | 122, 832 |
| MaeIII | GTNAC | 4 cut(s) | 8, 127, 488, 904 |
| MalI | GATC | 5 cut(s) | 64, 427, 561, 642, 660 |
| MbiI | CCGCTC | 1 cut(s) | 444 |
| MboI | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| MboII | GAAGA | 4 cut(s) | 178, 187, 413, 730 |
| MflI | RGATCY | 3 cut(s) | 62, 559, 640 |
| MhlI | GDGCHC | 2 cut(s) | 572, 827 |
| MlsI | TGGCCA | 1 cut(s) | 743 |
| MluCI | AATT | 1 cut(s) | 758 |
| MluNI | TGGCCA | 1 cut(s) | 743 |
| MlyI | GAGTC | 3 cut(s) | 857, 915, 1033 |
| Mox20I | TGGCCA | 1 cut(s) | 743 |
| MscI | TGGCCA | 1 cut(s) | 743 |
| MseI | TTAA | 1 cut(s) | 957 |
| MslI | CAYNNNNRTG | 2 cut(s) | 17, 749 |
| Msp20I | TGGCCA | 1 cut(s) | 743 |
| MspA1I | CMGCKG | 4 cut(s) | 190, 297, 433, 602 |
| MspI | CCGG | 4 cut(s) | 66, 79, 412, 849 |
| MspR9I | CCNGG | 5 cut(s) | 67, 412, 566, 615, 633 |
| MvaI | CCWGG | 3 cut(s) | 566, 615, 633 |
| MvnI | CGCG | 3 cut(s) | 259, 439, 820 |
| NciI | CCSGG | 2 cut(s) | 67, 412 |
| NdeII | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| NheI | GCTAGC | 3 cut(s) | 913, 970, 1031 |
| NlaIII | CATG | 2 cut(s) | 36, 373 |
| NlaIV | GGNNCC | 2 cut(s) | 561, 582 |
| NmuCI | GTSAC | 3 cut(s) | 8, 127, 488 |
| NspI | RCATGY | 2 cut(s) | 36, 373 |
| PaeI | GCATGC | 2 cut(s) | 36, 373 |
| PaeR7I | CTCGAG | 1 cut(s) | 27 |
| PaqCI | CACCTGC | 1 cut(s) | 727 |
| PasI | CCCWGGG | 1 cut(s) | 565 |
| PinAI | ACCGGT | 1 cut(s) | 848 |
| PleI | GAGTC | 3 cut(s) | 857, 915, 1033 |
| PpsI | GAGTC | 3 cut(s) | 857, 915, 1033 |
| PpuMI | RGGWCCY | 1 cut(s) | 580 |
| PshAI | GACNNNNGTC | 1 cut(s) | 486 |
| Psp5II | RGGWCCY | 1 cut(s) | 580 |
| Psp6I | CCWGG | 3 cut(s) | 564, 613, 631 |
| PspEI | GGTNACC | 1 cut(s) | 127 |
| PspFI | CCCAGC | 3 cut(s) | 132, 388, 799 |
| PspGI | CCWGG | 3 cut(s) | 564, 613, 631 |
| PspN4I | GGNNCC | 2 cut(s) | 561, 582 |
| PspPI | GGNCC | 4 cut(s) | 481, 580, 673, 727 |
| PspPPI | RGGWCCY | 1 cut(s) | 580 |
| PspXI | VCTCGAGB | 1 cut(s) | 27 |
| PstI | CTGCAG | 2 cut(s) | 244, 252 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1013 |
| PsuI | RGATCY | 3 cut(s) | 62, 559, 640 |
| PvuII | CAGCTG | 1 cut(s) | 602 |
| RseI | CAYNNNNRTG | 2 cut(s) | 17, 749 |
| SaqAI | TTAA | 1 cut(s) | 957 |
| Sau3AI | GATC | 5 cut(s) | 62, 425, 559, 640, 658 |
| Sau96I | GGNCC | 4 cut(s) | 481, 580, 673, 727 |
| SchI | GAGTC | 3 cut(s) | 857, 915, 1033 |
| ScrFI | CCNGG | 5 cut(s) | 67, 412, 566, 615, 633 |
| SduI | GDGCHC | 2 cut(s) | 572, 827 |
| SexAI | ACCWGGT | 1 cut(s) | 631 |
| SfaNI | GCATC | 3 cut(s) | 251, 299, 464 |
| SfcI | CTRYAG | 4 cut(s) | 45, 240, 248, 597 |
| Sfr274I | CTCGAG | 1 cut(s) | 27 |
| SinI | GGWCC | 3 cut(s) | 481, 580, 673 |
| SlaI | CTCGAG | 1 cut(s) | 27 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 17, 749 |
| SmlI | CTYRAG | 2 cut(s) | 27, 395 |
| SmoI | CTYRAG | 2 cut(s) | 27, 395 |
| SphI | GCATGC | 2 cut(s) | 36, 373 |
| Sse9I | AATT | 1 cut(s) | 758 |
| SspI | AATATT | 1 cut(s) | 94 |
| SspMI | CTAG | 6 cut(s) | 38, 467, 552, 914, 971, 1032 |
| StyD4I | CCNGG | 5 cut(s) | 65, 410, 564, 613, 631 |
| TaaI | ACNGT | 1 cut(s) | 949 |
| TaiI | ACGT | 2 cut(s) | 125, 835 |
| TaqI | TCGA | 3 cut(s) | 28, 53, 762 |
| TaqII | GACCGA | 2 cut(s) | 115, 737 |
| TasI | AATT | 1 cut(s) | 758 |
| TauI | GCSGC | 3 cut(s) | 444, 476, 650 |
| Tru1I | TTAA | 1 cut(s) | 957 |
| Tru9I | TTAA | 1 cut(s) | 957 |
| TscAI | CASTG | 1 cut(s) | 491 |
| TseFI | GTSAC | 3 cut(s) | 8, 127, 488 |
| TseI | GCWGC | 9 cut(s) | 187, 209, 239, 294, 303, 332, 357, 366, 618 |
| Tsp45I | GTSAC | 3 cut(s) | 8, 127, 488 |
| TspDTI | ATGAA | 2 cut(s) | 920, 980 |
| TspGWI | ACGGA | 2 cut(s) | 686, 943 |
| TspRI | CASTG | 1 cut(s) | 491 |
| VpaK11BI | GGWCC | 3 cut(s) | 481, 580, 673 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 680 |
| XapI | RAATTY | 1 cut(s) | 758 |
| XbaI | TCTAGA | 1 cut(s) | 466 |
| XceI | RCATGY | 2 cut(s) | 36, 373 |
| XhoI | CTCGAG | 1 cut(s) | 27 |
| XmiI | GTMKAC | 1 cut(s) | 838 |
| XspI | CTAG | 6 cut(s) | 38, 467, 552, 914, 971, 1032 |