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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 132 |
| AciI | CCGC | 5 cut(s) | 11, 57, 130, 171, 182 |
| AclWI | GGATC | 2 cut(s) | 34, 67 |
| AcoI | YGGCCR | 2 cut(s) | 127, 172 |
| AfaI | GTAC | 1 cut(s) | 42 |
| AflIII | ACRYGT | 1 cut(s) | 141 |
| AjnI | CCWGG | 2 cut(s) | 22, 213 |
| Alw26I | GTCTC | 1 cut(s) | 113 |
| AlwI | GGATC | 2 cut(s) | 34, 67 |
| AoxI | GGCC | 4 cut(s) | 20, 106, 127, 172 |
| ApeKI | GCWGC | 3 cut(s) | 4, 7, 208 |
| AspLEI | GCGC | 2 cut(s) | 97, 134 |
| AsuC2I | CCSGG | 1 cut(s) | 186 |
| AsuHPI | GGTGA | 1 cut(s) | 26 |
| BbvI | GCAGC | 3 cut(s) | 16, 19, 220 |
| BccI | CCATC | 1 cut(s) | 119 |
| BciT130I | CCWGG | 2 cut(s) | 24, 215 |
| BcnI | CCSGG | 1 cut(s) | 186 |
| BcoDI | GTCTC | 1 cut(s) | 113 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 19 |
| BisI | GCNGC | 6 cut(s) | 5, 8, 11, 130, 172, 209 |
| BlsI | GCNGC | 6 cut(s) | 6, 9, 12, 131, 173, 210 |
| Bme1390I | CCNGG | 3 cut(s) | 24, 186, 215 |
| BmrFI | CCNGG | 3 cut(s) | 24, 186, 215 |
| BpuMI | CCSGG | 1 cut(s) | 186 |
| BsaJI | CCNNGG | 1 cut(s) | 214 |
| Bse1I | ACTGG | 1 cut(s) | 58 |
| BseBI | CCWGG | 2 cut(s) | 24, 215 |
| BseDI | CCNNGG | 1 cut(s) | 214 |
| BseNI | ACTGG | 1 cut(s) | 58 |
| BseRI | GAGGAG | 2 cut(s) | 102, 105 |
| BseXI | GCAGC | 3 cut(s) | 16, 19, 220 |
| Bsh1236I | CGCG | 1 cut(s) | 132 |
| Bsh1285I | CGRYCG | 1 cut(s) | 182 |
| BshFI | GGCC | 4 cut(s) | 22, 108, 129, 174 |
| BsiEI | CGRYCG | 1 cut(s) | 182 |
| BsiSI | CCGG | 1 cut(s) | 186 |
| BsmAI | GTCTC | 1 cut(s) | 113 |
| BsnI | GGCC | 4 cut(s) | 22, 108, 129, 174 |
| Bsp143I | GATC | 2 cut(s) | 26, 59 |
| BspACI | CCGC | 5 cut(s) | 11, 57, 130, 171, 182 |
| BspANI | GGCC | 4 cut(s) | 22, 108, 129, 174 |
| BspFNI | CGCG | 1 cut(s) | 132 |
| BspHI | TCATGA | 1 cut(s) | 136 |
| BspPI | GGATC | 2 cut(s) | 34, 67 |
| BsrI | ACTGG | 1 cut(s) | 58 |
| BssECI | CCNNGG | 1 cut(s) | 214 |
| BssMI | GATC | 2 cut(s) | 26, 59 |
| Bst2UI | CCWGG | 2 cut(s) | 24, 215 |
| BstC8I | GCNNGC | 1 cut(s) | 198 |
| BstFNI | CGCG | 1 cut(s) | 132 |
| BstHHI | GCGC | 2 cut(s) | 97, 134 |
| BstKTI | GATC | 2 cut(s) | 29, 62 |
| BstMAI | GTCTC | 1 cut(s) | 113 |
| BstMBI | GATC | 2 cut(s) | 26, 59 |
| BstMCI | CGRYCG | 1 cut(s) | 182 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 10, 19 |
| BstNI | CCWGG | 2 cut(s) | 24, 215 |
| BstSCI | CCNGG | 3 cut(s) | 22, 184, 213 |
| BstUI | CGCG | 1 cut(s) | 132 |
| BstV1I | GCAGC | 3 cut(s) | 16, 19, 220 |
| BstX2I | RGATCY | 2 cut(s) | 26, 59 |
| BstYI | RGATCY | 2 cut(s) | 26, 59 |
| BsuRI | GGCC | 4 cut(s) | 22, 108, 129, 174 |
| BtsIMutI | CAGTG | 1 cut(s) | 51 |
| Cac8I | GCNNGC | 1 cut(s) | 198 |
| CciI | TCATGA | 1 cut(s) | 136 |
| CfoI | GCGC | 2 cut(s) | 97, 134 |
| Csp6I | GTAC | 1 cut(s) | 41 |
| CviAII | CATG | 2 cut(s) | 38, 137 |
| CviJI | RGCY | 5 cut(s) | 10, 22, 108, 129, 174 |
| CviKI_1 | RGCY | 5 cut(s) | 10, 22, 108, 129, 174 |
| CviQI | GTAC | 1 cut(s) | 41 |
| DpnI | GATC | 2 cut(s) | 28, 61 |
| DpnII | GATC | 2 cut(s) | 26, 59 |
| EaeI | YGGCCR | 2 cut(s) | 127, 172 |
| EciI | GGCGGA | 1 cut(s) | 72 |
| EcoRII | CCWGG | 2 cut(s) | 22, 213 |
| FaeI | CATG | 2 cut(s) | 41, 140 |
| FaiI | YATR | 3 cut(s) | 39, 67, 138 |
| FatI | CATG | 2 cut(s) | 37, 136 |
| Fnu4HI | GCNGC | 6 cut(s) | 5, 8, 11, 130, 172, 209 |
| Fsp4HI | GCNGC | 6 cut(s) | 5, 8, 11, 130, 172, 209 |
| GlaI | GCGC | 2 cut(s) | 96, 133 |
| GluI | GCNGC | 6 cut(s) | 5, 8, 11, 130, 172, 209 |
| HaeIII | GGCC | 4 cut(s) | 22, 108, 129, 174 |
| HapII | CCGG | 1 cut(s) | 186 |
| HhaI | GCGC | 2 cut(s) | 97, 134 |
| Hin1II | CATG | 2 cut(s) | 41, 140 |
| Hin6I | GCGC | 2 cut(s) | 95, 132 |
| HinP1I | GCGC | 2 cut(s) | 95, 132 |
| HpaII | CCGG | 1 cut(s) | 186 |
| HphI | GGTGA | 1 cut(s) | 26 |
| Hpy188III | TCNNGA | 1 cut(s) | 137 |
| HpyAV | CCTTC | 1 cut(s) | 98 |
| HpyCH4IV | ACGT | 1 cut(s) | 143 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 10, 19 |
| HpySE526I | ACGT | 1 cut(s) | 143 |
| Hsp92II | CATG | 2 cut(s) | 41, 140 |
| HspAI | GCGC | 2 cut(s) | 95, 132 |
| Kzo9I | GATC | 2 cut(s) | 26, 59 |
| LmnI | GCTCC | 1 cut(s) | 92 |
| LpnPI | CCDG | 8 cut(s) | 4, 9, 36, 39, 83, 129, 199, 200 |
| Lsp1109I | GCAGC | 3 cut(s) | 16, 19, 220 |
| MaeII | ACGT | 1 cut(s) | 143 |
| MaeIII | GTNAC | 2 cut(s) | 32, 202 |
| MalI | GATC | 2 cut(s) | 28, 61 |
| MboI | GATC | 2 cut(s) | 26, 59 |
| MboII | GAAGA | 1 cut(s) | 54 |
| MflI | RGATCY | 2 cut(s) | 26, 59 |
| MnlI | CCTC | 3 cut(s) | 80, 83, 143 |
| MspI | CCGG | 1 cut(s) | 186 |
| MspR9I | CCNGG | 3 cut(s) | 24, 186, 215 |
| MvaI | CCWGG | 2 cut(s) | 24, 215 |
| MvnI | CGCG | 1 cut(s) | 132 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 10, 19 |
| NciI | CCSGG | 1 cut(s) | 186 |
| NdeII | GATC | 2 cut(s) | 26, 59 |
| NlaIII | CATG | 2 cut(s) | 41, 140 |
| NmuCI | GTSAC | 2 cut(s) | 32, 202 |
| PagI | TCATGA | 1 cut(s) | 136 |
| PkrI | GCNGC | 6 cut(s) | 6, 9, 12, 131, 173, 210 |
| Psp6I | CCWGG | 2 cut(s) | 22, 213 |
| PspGI | CCWGG | 2 cut(s) | 22, 213 |
| PsuI | RGATCY | 2 cut(s) | 26, 59 |
| RsaI | GTAC | 1 cut(s) | 42 |
| RsaNI | GTAC | 1 cut(s) | 41 |
| SatI | GCNGC | 6 cut(s) | 5, 8, 11, 130, 172, 209 |
| Sau3AI | GATC | 2 cut(s) | 26, 59 |
| ScrFI | CCNGG | 3 cut(s) | 24, 186, 215 |
| SetI | ASST | 1 cut(s) | 146 |
| SsiI | CCGC | 5 cut(s) | 11, 57, 130, 171, 182 |
| StyD4I | CCNGG | 3 cut(s) | 22, 184, 213 |
| TaiI | ACGT | 1 cut(s) | 146 |
| TauI | GCSGC | 3 cut(s) | 13, 132, 174 |
| TscAI | CASTG | 1 cut(s) | 58 |
| TseFI | GTSAC | 2 cut(s) | 32, 202 |
| TseI | GCWGC | 3 cut(s) | 4, 7, 208 |
| Tsp45I | GTSAC | 2 cut(s) | 32, 202 |
| TspDTI | ATGAA | 1 cut(s) | 54 |
| TspRI | CASTG | 1 cut(s) | 58 |