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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 47 |
| Acc36I | ACCTGC | 1 cut(s) | 247 |
| AccII | CGCG | 1 cut(s) | 90 |
| AciI | CCGC | 6 cut(s) | 74, 88, 120, 145, 149, 312 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| AjnI | CCWGG | 2 cut(s) | 151, 347 |
| AleI | CACNNNNGTG | 1 cut(s) | 269 |
| AluBI | AGCT | 3 cut(s) | 181, 289, 357 |
| AluI | AGCT | 3 cut(s) | 181, 289, 357 |
| Alw21I | GWGCWC | 1 cut(s) | 347 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| AoxI | GGCC | 4 cut(s) | 154, 240, 246, 261 |
| ApeKI | GCWGC | 1 cut(s) | 141 |
| AspLEI | GCGC | 1 cut(s) | 92 |
| AspS9I | GGNCC | 1 cut(s) | 247 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 320 |
| AsuHPI | GGTGA | 2 cut(s) | 91, 134 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| Bbv12I | GWGCWC | 1 cut(s) | 347 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 27, 61 |
| BciT130I | CCWGG | 2 cut(s) | 153, 349 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BfuAI | ACCTGC | 1 cut(s) | 247 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 2 cut(s) | 142, 145 |
| BlsI | GCNGC | 2 cut(s) | 143, 146 |
| Bme1390I | CCNGG | 5 cut(s) | 107, 117, 153, 320, 349 |
| BmgT120I | GGNCC | 1 cut(s) | 247 |
| BmrFI | CCNGG | 5 cut(s) | 107, 117, 153, 320, 349 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BsaJI | CCNNGG | 3 cut(s) | 105, 115, 243 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| Bse1I | ACTGG | 1 cut(s) | 43 |
| BseBI | CCWGG | 2 cut(s) | 153, 349 |
| BseDI | CCNNGG | 3 cut(s) | 105, 115, 243 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| BseNI | ACTGG | 1 cut(s) | 43 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| BsiHKAI | GWGCWC | 1 cut(s) | 347 |
| BsiSI | CCGG | 3 cut(s) | 107, 117, 320 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsnI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| Bsp1286I | GDGCHC | 1 cut(s) | 347 |
| BspACI | CCGC | 6 cut(s) | 74, 88, 120, 145, 149, 312 |
| BspANI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspMI | ACCTGC | 1 cut(s) | 247 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrI | ACTGG | 1 cut(s) | 43 |
| BssECI | CCNNGG | 3 cut(s) | 105, 115, 243 |
| Bst2UI | CCWGG | 2 cut(s) | 153, 349 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 149, 183, 332 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstHHI | GCGC | 1 cut(s) | 92 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 87, 153, 331 |
| BstNI | CCWGG | 2 cut(s) | 153, 349 |
| BstSCI | CCNGG | 5 cut(s) | 105, 115, 151, 318, 347 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BsuRI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| BveI | ACCTGC | 1 cut(s) | 247 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 149, 183, 332 |
| CfoI | GCGC | 1 cut(s) | 92 |
| Cfr13I | GGNCC | 1 cut(s) | 247 |
| CviAII | CATG | 1 cut(s) | 339 |
| CviJI | RGCY | 9 cut(s) | 144, 156, 181, 185, 242, 248, 263, 289, 357 |
| CviKI_1 | RGCY | 9 cut(s) | 144, 156, 181, 185, 242, 248, 263, 289, 357 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 47 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 47 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EcoRII | CCWGG | 2 cut(s) | 151, 347 |
| FaeI | CATG | 1 cut(s) | 342 |
| FaiI | YATR | 3 cut(s) | 175, 177, 340 |
| FatI | CATG | 1 cut(s) | 338 |
| FauI | CCCGC | 1 cut(s) | 156 |
| Fnu4HI | GCNGC | 2 cut(s) | 142, 145 |
| Fsp4HI | GCNGC | 2 cut(s) | 142, 145 |
| GlaI | GCGC | 1 cut(s) | 91 |
| GluI | GCNGC | 2 cut(s) | 142, 145 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| HaeIII | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| HapII | CCGG | 3 cut(s) | 107, 117, 320 |
| HhaI | GCGC | 1 cut(s) | 92 |
| Hin1II | CATG | 1 cut(s) | 342 |
| Hin6I | GCGC | 1 cut(s) | 90 |
| HinP1I | GCGC | 1 cut(s) | 90 |
| HinfI | GANTC | 1 cut(s) | 193 |
| HpaII | CCGG | 3 cut(s) | 107, 117, 320 |
| HphI | GGTGA | 2 cut(s) | 91, 134 |
| Hpy166II | GTNNAC | 2 cut(s) | 112, 221 |
| Hpy8I | GTNNAC | 2 cut(s) | 112, 221 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 87, 153, 331 |
| Hsp92II | CATG | 1 cut(s) | 342 |
| HspAI | GCGC | 1 cut(s) | 90 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 342 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| LweI | GCATC | 1 cut(s) | 90 |
| MaeIII | GTNAC | 1 cut(s) | 273 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MhlI | GDGCHC | 1 cut(s) | 347 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MnlI | CCTC | 3 cut(s) | 173, 178, 238 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 357 |
| MspI | CCGG | 3 cut(s) | 107, 117, 320 |
| MspR9I | CCNGG | 5 cut(s) | 107, 117, 153, 320, 349 |
| MvaI | CCWGG | 2 cut(s) | 153, 349 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 87, 153, 331 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 320 |
| NlaIII | CATG | 1 cut(s) | 342 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 273 |
| OliI | CACNNNNGTG | 1 cut(s) | 269 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 193 |
| PkrI | GCNGC | 2 cut(s) | 143, 146 |
| Psp6I | CCWGG | 2 cut(s) | 151, 347 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspGI | CCWGG | 2 cut(s) | 151, 347 |
| PspPI | GGNCC | 1 cut(s) | 247 |
| PvuII | CAGCTG | 1 cut(s) | 357 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SatI | GCNGC | 2 cut(s) | 142, 145 |
| Sau96I | GGNCC | 1 cut(s) | 247 |
| ScrFI | CCNGG | 5 cut(s) | 107, 117, 153, 320, 349 |
| SduI | GDGCHC | 1 cut(s) | 347 |
| SetI | ASST | 5 cut(s) | 165, 183, 261, 291, 359 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SsiI | CCGC | 6 cut(s) | 74, 88, 120, 145, 149, 312 |
| StyD4I | CCNGG | 5 cut(s) | 105, 115, 151, 318, 347 |
| TaqII | GACCGA | 1 cut(s) | 37 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TfiI | GAWTC | 1 cut(s) | 193 |
| TseFI | GTSAC | 1 cut(s) | 273 |
| TseI | GCWGC | 1 cut(s) | 141 |
| Tsp45I | GTSAC | 1 cut(s) | 273 |
| TspDTI | ATGAA | 1 cut(s) | 42 |