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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 535, 635 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 587 |
| Acc36I | ACCTGC | 2 cut(s) | 535, 635 |
| Acc65I | GGTACC | 1 cut(s) | 367 |
| AccB1I | GGYRCC | 2 cut(s) | 367, 647 |
| AccBSI | CCGCTC | 1 cut(s) | 492 |
| AccII | CGCG | 1 cut(s) | 207 |
| AciI | CCGC | 9 cut(s) | 92, 110, 156, 179, 360, 490, 538, 566, 638 |
| AclI | AACGTT | 1 cut(s) | 6 |
| AclWI | GGATC | 1 cut(s) | 189 |
| AcoI | YGGCCR | 1 cut(s) | 249 |
| AfaI | GTAC | 2 cut(s) | 369, 396 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 244, 356, 537, 538, 565, 598, 640 |
| AgsI | TTSAA | 3 cut(s) | 43, 271, 403 |
| AhlI | ACTAGT | 1 cut(s) | 139 |
| AjnI | CCWGG | 5 cut(s) | 84, 533, 561, 597, 683 |
| AluBI | AGCT | 2 cut(s) | 169, 277 |
| AluI | AGCT | 2 cut(s) | 169, 277 |
| Alw21I | GWGCWC | 1 cut(s) | 335 |
| AlwI | GGATC | 1 cut(s) | 189 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 690 |
| AoxI | GGCC | 5 cut(s) | 234, 249, 546, 569, 686 |
| ApaI | GGGCCC | 1 cut(s) | 573 |
| ApeKI | GCWGC | 1 cut(s) | 129 |
| Asp718I | GGTACC | 1 cut(s) | 367 |
| AspLEI | GCGC | 3 cut(s) | 37, 207, 313 |
| AspS9I | GGNCC | 8 cut(s) | 68, 88, 235, 428, 547, 569, 570, 687 |
| AsuC2I | CCSGG | 2 cut(s) | 73, 635 |
| AsuHPI | GGTGA | 2 cut(s) | 590, 648 |
| AvaII | GGWCC | 3 cut(s) | 68, 88, 428 |
| BaeGI | GKGCMC | 1 cut(s) | 573 |
| BalI | TGGCCA | 1 cut(s) | 251 |
| BanI | GGYRCC | 2 cut(s) | 367, 647 |
| BanII | GRGCYC | 1 cut(s) | 573 |
| Bbv12I | GWGCWC | 1 cut(s) | 335 |
| BbvI | GCAGC | 1 cut(s) | 141 |
| BciT130I | CCWGG | 5 cut(s) | 86, 535, 563, 599, 685 |
| BcnI | CCSGG | 2 cut(s) | 73, 635 |
| BcuI | ACTAGT | 1 cut(s) | 139 |
| BfaI | CTAG | 3 cut(s) | 140, 507, 617 |
| BfuAI | ACCTGC | 2 cut(s) | 535, 635 |
| BisI | GCNGC | 2 cut(s) | 130, 156 |
| BlpI | GCTNAGC | 1 cut(s) | 117 |
| BlsI | GCNGC | 2 cut(s) | 131, 157 |
| BmcAI | AGTACT | 1 cut(s) | 396 |
| Bme1390I | CCNGG | 7 cut(s) | 73, 86, 535, 563, 599, 635, 685 |
| Bme18I | GGWCC | 3 cut(s) | 68, 88, 428 |
| BmgT120I | GGNCC | 8 cut(s) | 68, 88, 235, 428, 547, 569, 570, 687 |
| BmiI | GGNNCC | 8 cut(s) | 69, 70, 90, 369, 429, 570, 571, 649 |
| BmrFI | CCNGG | 7 cut(s) | 73, 86, 535, 563, 599, 635, 685 |
| BmsI | GCATC | 2 cut(s) | 378, 597 |
| BpmI | CTGGAG | 4 cut(s) | 43, 68, 311, 545 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 117 |
| BpuMI | CCSGG | 2 cut(s) | 73, 635 |
| BsaJI | CCNNGG | 3 cut(s) | 71, 231, 533 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 455, 485 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 244, 356, 537, 538, 565, 598, 640 |
| Bse1I | ACTGG | 5 cut(s) | 54, 180, 294, 549, 580 |
| Bse3DI | GCAATG | 1 cut(s) | 150 |
| BseBI | CCWGG | 5 cut(s) | 86, 535, 563, 599, 685 |
| BseDI | CCNNGG | 3 cut(s) | 71, 231, 533 |
| BseGI | GGATG | 1 cut(s) | 612 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 244, 356, 537, 538, 565, 598, 640 |
| BseMI | GCAATG | 1 cut(s) | 150 |
| BseMII | CTCAG | 2 cut(s) | 131, 504 |
| BseNI | ACTGG | 5 cut(s) | 54, 180, 294, 549, 580 |
| BseSI | GKGCMC | 1 cut(s) | 573 |
| BseXI | GCAGC | 1 cut(s) | 141 |
| BseYI | CCCAGC | 2 cut(s) | 307, 549 |
| BsgI | GTGCAG | 2 cut(s) | 164, 238 |
| Bsh1236I | CGCG | 1 cut(s) | 207 |
| BshFI | GGCC | 5 cut(s) | 236, 251, 548, 571, 688 |
| BshNI | GGYRCC | 2 cut(s) | 367, 647 |
| BsiHKAI | GWGCWC | 1 cut(s) | 335 |
| BsiSI | CCGG | 2 cut(s) | 73, 634 |
| BslFI | GGGAC | 3 cut(s) | 74, 81, 441 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 244, 356, 537, 538, 565, 598, 640 |
| BsmFI | GGGAC | 3 cut(s) | 74, 81, 441 |
| BsnI | GGCC | 5 cut(s) | 236, 251, 548, 571, 688 |
| Bsp120I | GGGCCC | 1 cut(s) | 569 |
| Bsp1286I | GDGCHC | 2 cut(s) | 335, 573 |
| Bsp143I | GATC | 2 cut(s) | 181, 315 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 117 |
| BspACI | CCGC | 9 cut(s) | 92, 110, 156, 179, 360, 490, 538, 566, 638 |
| BspANI | GGCC | 5 cut(s) | 236, 251, 548, 571, 688 |
| BspCNI | CTCAG | 2 cut(s) | 130, 505 |
| BspFNI | CGCG | 1 cut(s) | 207 |
| BspLI | GGNNCC | 8 cut(s) | 69, 70, 90, 369, 429, 570, 571, 649 |
| BspMI | ACCTGC | 2 cut(s) | 535, 635 |
| BspPI | GGATC | 1 cut(s) | 189 |
| BspT107I | GGYRCC | 2 cut(s) | 367, 647 |
| BsrBI | CCGCTC | 1 cut(s) | 492 |
| BsrDI | GCAATG | 1 cut(s) | 150 |
| BsrI | ACTGG | 5 cut(s) | 54, 180, 294, 549, 580 |
| BssECI | CCNNGG | 3 cut(s) | 71, 231, 533 |
| BssMI | GATC | 2 cut(s) | 181, 315 |
| Bst2UI | CCWGG | 5 cut(s) | 86, 535, 563, 599, 685 |
| Bst4CI | ACNGT | 3 cut(s) | 32, 399, 589 |
| BstC8I | GCNNGC | 2 cut(s) | 171, 209 |
| BstDEI | CTNAG | 2 cut(s) | 117, 513 |
| BstF5I | GGATG | 1 cut(s) | 612 |
| BstFNI | CGCG | 1 cut(s) | 207 |
| BstHHI | GCGC | 3 cut(s) | 37, 207, 313 |
| BstKTI | GATC | 2 cut(s) | 184, 318 |
| BstMBI | GATC | 2 cut(s) | 181, 315 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 293, 308, 339 |
| BstNI | CCWGG | 5 cut(s) | 86, 535, 563, 599, 685 |
| BstSCI | CCNGG | 7 cut(s) | 71, 84, 533, 561, 597, 633, 683 |
| BstSLI | GKGCMC | 1 cut(s) | 573 |
| BstUI | CGCG | 1 cut(s) | 207 |
| BstV1I | GCAGC | 1 cut(s) | 141 |
| BstX2I | RGATCY | 1 cut(s) | 181 |
| BstYI | RGATCY | 1 cut(s) | 181 |
| BsuRI | GGCC | 5 cut(s) | 236, 251, 548, 571, 688 |
| BtsCI | GGATG | 1 cut(s) | 612 |
| BtsIMutI | CAGTG | 4 cut(s) | 47, 173, 287, 573 |
| BveI | ACCTGC | 2 cut(s) | 535, 635 |
| Cac8I | GCNNGC | 2 cut(s) | 171, 209 |
| CaiI | CAGNNNCTG | 1 cut(s) | 690 |
| CfoI | GCGC | 3 cut(s) | 37, 207, 313 |
| Cfr13I | GGNCC | 8 cut(s) | 68, 88, 235, 428, 547, 569, 570, 687 |
| CseI | GACGC | 3 cut(s) | 348, 590, 669 |
| Csp6I | GTAC | 2 cut(s) | 368, 395 |
| CviAII | CATG | 1 cut(s) | 64 |
| CviQI | GTAC | 2 cut(s) | 368, 395 |
| DdeI | CTNAG | 2 cut(s) | 117, 513 |
| DpnI | GATC | 2 cut(s) | 183, 317 |
| DpnII | GATC | 2 cut(s) | 181, 315 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 587 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 587 |
| EaeI | YGGCCR | 1 cut(s) | 249 |
| EciI | GGCGGA | 3 cut(s) | 194, 375, 653 |
| Eco24I | GRGCYC | 1 cut(s) | 573 |
| Eco47I | GGWCC | 3 cut(s) | 68, 88, 428 |
| EcoO109I | RGGNCCY | 4 cut(s) | 68, 88, 569, 687 |
| EcoRII | CCWGG | 5 cut(s) | 84, 533, 561, 597, 683 |
| EcoT38I | GRGCYC | 1 cut(s) | 573 |
| FaeI | CATG | 1 cut(s) | 67 |
| FaiI | YATR | 5 cut(s) | 65, 161, 165, 374, 383 |
| FaqI | GGGAC | 3 cut(s) | 74, 81, 441 |
| FatI | CATG | 1 cut(s) | 63 |
| FauI | CCCGC | 3 cut(s) | 99, 531, 559 |
| Fnu4HI | GCNGC | 2 cut(s) | 130, 156 |
| FokI | GGATG | 1 cut(s) | 619 |
| FriOI | GRGCYC | 1 cut(s) | 573 |
| Fsp4HI | GCNGC | 2 cut(s) | 130, 156 |
| FspBI | CTAG | 3 cut(s) | 140, 507, 617 |
| GlaI | GCGC | 3 cut(s) | 36, 206, 312 |
| GluI | GCNGC | 2 cut(s) | 130, 156 |
| GsaI | CCCAGC | 2 cut(s) | 311, 553 |
| GsuI | CTGGAG | 4 cut(s) | 43, 68, 311, 545 |
| HaeIII | GGCC | 5 cut(s) | 236, 251, 548, 571, 688 |
| HapII | CCGG | 2 cut(s) | 73, 634 |
| HgaI | GACGC | 3 cut(s) | 348, 590, 669 |
| HhaI | GCGC | 3 cut(s) | 37, 207, 313 |
| Hin1II | CATG | 1 cut(s) | 67 |
| Hin6I | GCGC | 3 cut(s) | 35, 205, 311 |
| HinP1I | GCGC | 3 cut(s) | 35, 205, 311 |
| HincII | GTYRAC | 4 cut(s) | 10, 28, 55, 347 |
| HindII | GTYRAC | 4 cut(s) | 10, 28, 55, 347 |
| HinfI | GANTC | 1 cut(s) | 267 |
| HpaI | GTTAAC | 2 cut(s) | 10, 28 |
| HpaII | CCGG | 2 cut(s) | 73, 634 |
| HphI | GGTGA | 2 cut(s) | 590, 648 |
| Hpy166II | GTNNAC | 4 cut(s) | 10, 28, 55, 347 |
| Hpy188III | TCNNGA | 1 cut(s) | 264 |
| Hpy8I | GTNNAC | 4 cut(s) | 10, 28, 55, 347 |
| HpyCH4III | ACNGT | 3 cut(s) | 32, 399, 589 |
| HpyCH4IV | ACGT | 1 cut(s) | 6 |
| HpyCH4V | TGCA | 4 cut(s) | 145, 219, 391, 610 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 293, 308, 339 |
| HpyF3I | CTNAG | 2 cut(s) | 117, 513 |
| HpySE526I | ACGT | 1 cut(s) | 6 |
| Hsp92II | CATG | 1 cut(s) | 67 |
| HspAI | GCGC | 3 cut(s) | 35, 205, 311 |
| KflI | GGGWCCC | 1 cut(s) | 68 |
| KpnI | GGTACC | 1 cut(s) | 371 |
| KspAI | GTTAAC | 2 cut(s) | 10, 28 |
| Kzo9I | GATC | 2 cut(s) | 181, 315 |
| LmnI | GCTCC | 2 cut(s) | 330, 457 |
| Lsp1109I | GCAGC | 1 cut(s) | 141 |
| LweI | GCATC | 2 cut(s) | 378, 597 |
| MaeI | CTAG | 3 cut(s) | 140, 507, 617 |
| MaeII | ACGT | 1 cut(s) | 6 |
| MaeIII | GTNAC | 2 cut(s) | 471, 578 |
| MalI | GATC | 2 cut(s) | 183, 317 |
| MbiI | CCGCTC | 1 cut(s) | 492 |
| MboI | GATC | 2 cut(s) | 181, 315 |
| MflI | RGATCY | 1 cut(s) | 181 |
| MhlI | GDGCHC | 2 cut(s) | 335, 573 |
| MlsI | TGGCCA | 1 cut(s) | 251 |
| MluNI | TGGCCA | 1 cut(s) | 251 |
| MmeI | TCCRAC | 1 cut(s) | 435 |
| Mox20I | TGGCCA | 1 cut(s) | 251 |
| MscI | TGGCCA | 1 cut(s) | 251 |
| MseI | TTAA | 2 cut(s) | 9, 27 |
| MslI | CAYNNNNRTG | 2 cut(s) | 257, 386 |
| Msp20I | TGGCCA | 1 cut(s) | 251 |
| MspA1I | CMGCKG | 1 cut(s) | 110 |
| MspI | CCGG | 2 cut(s) | 73, 634 |
| MspR9I | CCNGG | 7 cut(s) | 73, 86, 535, 563, 599, 635, 685 |
| MvaI | CCWGG | 5 cut(s) | 86, 535, 563, 599, 685 |
| MvnI | CGCG | 1 cut(s) | 207 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 293, 308, 339 |
| NciI | CCSGG | 2 cut(s) | 73, 635 |
| NdeII | GATC | 2 cut(s) | 181, 315 |
| NlaIII | CATG | 1 cut(s) | 67 |
| NlaIV | GGNNCC | 8 cut(s) | 69, 70, 90, 369, 429, 570, 571, 649 |
| NmeAIII | GCCGAG | 1 cut(s) | 256 |
| NmuCI | GTSAC | 1 cut(s) | 578 |
| PaqCI | CACCTGC | 2 cut(s) | 535, 635 |
| PfeI | GAWTC | 1 cut(s) | 267 |
| PflFI | GACNNNGTC | 1 cut(s) | 342 |
| PfoI | TCCNGGA | 1 cut(s) | 597 |
| PkrI | GCNGC | 2 cut(s) | 131, 157 |
| PpuMI | RGGWCCY | 2 cut(s) | 68, 88 |
| Psp1406I | AACGTT | 1 cut(s) | 6 |
| Psp5II | RGGWCCY | 2 cut(s) | 68, 88 |
| Psp6I | CCWGG | 5 cut(s) | 84, 533, 561, 597, 683 |
| PspFI | CCCAGC | 2 cut(s) | 307, 549 |
| PspGI | CCWGG | 5 cut(s) | 84, 533, 561, 597, 683 |
| PspN4I | GGNNCC | 8 cut(s) | 69, 70, 90, 369, 429, 570, 571, 649 |
| PspOMI | GGGCCC | 1 cut(s) | 569 |
| PspPI | GGNCC | 8 cut(s) | 68, 88, 235, 428, 547, 569, 570, 687 |
| PspPPI | RGGWCCY | 2 cut(s) | 68, 88 |
| PstNI | CAGNNNCTG | 1 cut(s) | 690 |
| PsuI | RGATCY | 1 cut(s) | 181 |
| PsyI | GACNNNGTC | 1 cut(s) | 342 |
| RsaI | GTAC | 2 cut(s) | 369, 396 |
| RsaNI | GTAC | 2 cut(s) | 368, 395 |
| RseI | CAYNNNNRTG | 2 cut(s) | 257, 386 |
| SaqAI | TTAA | 2 cut(s) | 9, 27 |
| SatI | GCNGC | 2 cut(s) | 130, 156 |
| Sau3AI | GATC | 2 cut(s) | 181, 315 |
| Sau96I | GGNCC | 8 cut(s) | 68, 88, 235, 428, 547, 569, 570, 687 |
| ScaI | AGTACT | 1 cut(s) | 396 |
| ScrFI | CCNGG | 7 cut(s) | 73, 86, 535, 563, 599, 635, 685 |
| SduI | GDGCHC | 2 cut(s) | 335, 573 |
| SfaNI | GCATC | 2 cut(s) | 378, 597 |
| SinI | GGWCC | 3 cut(s) | 68, 88, 428 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 257, 386 |
| SpeI | ACTAGT | 1 cut(s) | 139 |
| SsiI | CCGC | 9 cut(s) | 92, 110, 156, 179, 360, 490, 538, 566, 638 |
| SspMI | CTAG | 3 cut(s) | 140, 507, 617 |
| StyD4I | CCNGG | 7 cut(s) | 71, 84, 533, 561, 597, 633, 683 |
| TaaI | ACNGT | 3 cut(s) | 32, 399, 589 |
| TaiI | ACGT | 1 cut(s) | 9 |
| TaqI | TCGA | 2 cut(s) | 318, 494 |
| TatI | WGTACW | 1 cut(s) | 394 |
| TauI | GCSGC | 1 cut(s) | 158 |
| TfiI | GAWTC | 1 cut(s) | 267 |
| Tru1I | TTAA | 2 cut(s) | 9, 27 |
| Tru9I | TTAA | 2 cut(s) | 9, 27 |
| TscAI | CASTG | 4 cut(s) | 54, 180, 294, 580 |
| TseFI | GTSAC | 1 cut(s) | 578 |
| TseI | GCWGC | 1 cut(s) | 129 |
| Tsp45I | GTSAC | 1 cut(s) | 578 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 343 |
| TspRI | CASTG | 4 cut(s) | 54, 180, 294, 580 |
| Tth111I | GACNNNGTC | 1 cut(s) | 342 |
| VpaK11BI | GGWCC | 3 cut(s) | 68, 88, 428 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 541 |
| XspI | CTAG | 3 cut(s) | 140, 507, 617 |
| ZrmI | AGTACT | 1 cut(s) | 396 |