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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 562 |
| Acc65I | GGTACC | 2 cut(s) | 547, 574 |
| AccB1I | GGYRCC | 2 cut(s) | 547, 574 |
| AccBSI | CCGCTC | 1 cut(s) | 672 |
| AccI | GTMKAC | 1 cut(s) | 526 |
| AccII | CGCG | 3 cut(s) | 71, 86, 242 |
| AclWI | GGATC | 1 cut(s) | 369 |
| AcoI | YGGCCR | 1 cut(s) | 276 |
| AcuI | CTGAAG | 4 cut(s) | 144, 216, 432, 714 |
| AfaI | GTAC | 3 cut(s) | 344, 549, 576 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 24, 183, 245, 424, 536, 676 |
| AflIII | ACRYGT | 1 cut(s) | 443 |
| AjnI | CCWGG | 3 cut(s) | 20, 515, 550 |
| AluBI | AGCT | 2 cut(s) | 106, 349 |
| AluI | AGCT | 2 cut(s) | 106, 349 |
| Alw21I | GWGCWC | 1 cut(s) | 262 |
| AlwI | GGATC | 1 cut(s) | 369 |
| AoxI | GGCC | 4 cut(s) | 276, 322, 474, 554 |
| ApeKI | GCWGC | 2 cut(s) | 306, 309 |
| Asp718I | GGTACC | 2 cut(s) | 547, 574 |
| AspLEI | GCGC | 3 cut(s) | 73, 88, 399 |
| AspS9I | GGNCC | 3 cut(s) | 46, 554, 608 |
| AsuC2I | CCSGG | 3 cut(s) | 275, 285, 707 |
| AsuHPI | GGTGA | 1 cut(s) | 166 |
| AsuNHI | GCTAGC | 1 cut(s) | 349 |
| AvaII | GGWCC | 2 cut(s) | 46, 608 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 531, 564 |
| BanI | GGYRCC | 2 cut(s) | 547, 574 |
| BbsI | GAAGAC | 1 cut(s) | 470 |
| Bbv12I | GWGCWC | 1 cut(s) | 262 |
| BbvCI | CCTCAGC | 1 cut(s) | 37 |
| BbvI | GCAGC | 2 cut(s) | 318, 321 |
| BccI | CCATC | 3 cut(s) | 87, 227, 325 |
| BciT130I | CCWGG | 3 cut(s) | 22, 517, 552 |
| BcnI | CCSGG | 3 cut(s) | 275, 285, 707 |
| BfaI | CTAG | 2 cut(s) | 350, 681 |
| BfmI | CTRYAG | 1 cut(s) | 30 |
| BfuAI | ACCTGC | 1 cut(s) | 562 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 26, 94 |
| BglII | AGATCT | 1 cut(s) | 328 |
| BisI | GCNGC | 5 cut(s) | 63, 74, 289, 307, 310 |
| BlsI | GCNGC | 5 cut(s) | 64, 75, 290, 308, 311 |
| Bme1390I | CCNGG | 6 cut(s) | 22, 275, 285, 517, 552, 707 |
| Bme18I | GGWCC | 2 cut(s) | 46, 608 |
| BmgT120I | GGNCC | 3 cut(s) | 46, 554, 608 |
| BmiI | GGNNCC | 3 cut(s) | 549, 576, 609 |
| BmrFI | CCNGG | 6 cut(s) | 22, 275, 285, 517, 552, 707 |
| BmrI | ACTGGG | 1 cut(s) | 125 |
| BmsI | GCATC | 2 cut(s) | 106, 558 |
| BmtI | GCTAGC | 1 cut(s) | 353 |
| BmuI | ACTGGG | 1 cut(s) | 125 |
| BpiI | GAAGAC | 1 cut(s) | 470 |
| BpmI | CTGGAG | 2 cut(s) | 38, 491 |
| Bpu10I | CCTNAGC | 1 cut(s) | 37 |
| BpuMI | CCSGG | 3 cut(s) | 275, 285, 707 |
| BsaAI | YACGTR | 1 cut(s) | 509 |
| BsaJI | CCNNGG | 4 cut(s) | 240, 273, 283, 551 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 24, 183, 245, 424, 536, 676 |
| Bse118I | RCCGGY | 2 cut(s) | 536, 556 |
| Bse1I | ACTGG | 2 cut(s) | 120, 360 |
| Bse3DI | GCAATG | 1 cut(s) | 706 |
| BseBI | CCWGG | 3 cut(s) | 22, 517, 552 |
| BseDI | CCNNGG | 4 cut(s) | 240, 273, 283, 551 |
| BseGI | GGATG | 1 cut(s) | 219 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 24, 183, 245, 424, 536, 676 |
| BseMI | GCAATG | 1 cut(s) | 706 |
| BseMII | CTCAG | 2 cut(s) | 51, 582 |
| BseNI | ACTGG | 2 cut(s) | 120, 360 |
| BseRI | GAGGAG | 2 cut(s) | 404, 407 |
| BseXI | GCAGC | 2 cut(s) | 318, 321 |
| BseYI | CCCAGC | 2 cut(s) | 487, 530 |
| Bsh1236I | CGCG | 3 cut(s) | 71, 86, 242 |
| BshFI | GGCC | 4 cut(s) | 278, 324, 476, 556 |
| BshNI | GGYRCC | 2 cut(s) | 547, 574 |
| BsiHKAI | GWGCWC | 1 cut(s) | 262 |
| BsiSI | CCGG | 5 cut(s) | 275, 285, 537, 557, 707 |
| BslFI | GGGAC | 3 cut(s) | 121, 621, 689 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 24, 183, 245, 424, 536, 676 |
| BsmFI | GGGAC | 3 cut(s) | 121, 621, 689 |
| BsnI | GGCC | 4 cut(s) | 278, 324, 476, 556 |
| Bsp1286I | GDGCHC | 1 cut(s) | 262 |
| Bsp143I | GATC | 4 cut(s) | 57, 159, 328, 361 |
| BspANI | GGCC | 4 cut(s) | 278, 324, 476, 556 |
| BspCNI | CTCAG | 2 cut(s) | 50, 583 |
| BspFNI | CGCG | 3 cut(s) | 71, 86, 242 |
| BspHI | TCATGA | 1 cut(s) | 438 |
| BspLI | GGNNCC | 3 cut(s) | 549, 576, 609 |
| BspMI | ACCTGC | 1 cut(s) | 562 |
| BspOI | GCTAGC | 1 cut(s) | 353 |
| BspPI | GGATC | 1 cut(s) | 369 |
| BspQI | GCTCTTC | 1 cut(s) | 192 |
| BspT107I | GGYRCC | 2 cut(s) | 547, 574 |
| BsrBI | CCGCTC | 1 cut(s) | 672 |
| BsrDI | GCAATG | 1 cut(s) | 706 |
| BsrFI | RCCGGY | 2 cut(s) | 536, 556 |
| BsrI | ACTGG | 2 cut(s) | 120, 360 |
| BssAI | RCCGGY | 2 cut(s) | 536, 556 |
| BssECI | CCNNGG | 4 cut(s) | 240, 273, 283, 551 |
| BssMI | GATC | 4 cut(s) | 57, 159, 328, 361 |
| Bst2UI | CCWGG | 3 cut(s) | 22, 517, 552 |
| Bst6I | CTCTTC | 2 cut(s) | 173, 192 |
| BstBAI | YACGTR | 1 cut(s) | 509 |
| BstC8I | GCNNGC | 6 cut(s) | 27, 204, 216, 351, 484, 500 |
| BstDEI | CTNAG | 2 cut(s) | 37, 591 |
| BstDSI | CCRYGG | 1 cut(s) | 240 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 181 |
| BstF5I | GGATG | 1 cut(s) | 219 |
| BstFNI | CGCG | 3 cut(s) | 71, 86, 242 |
| BstHHI | GCGC | 3 cut(s) | 73, 88, 399 |
| BstKTI | GATC | 4 cut(s) | 60, 162, 331, 364 |
| BstMBI | GATC | 4 cut(s) | 57, 159, 328, 361 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 26, 68, 94, 103, 488 |
| BstNI | CCWGG | 3 cut(s) | 22, 517, 552 |
| BstSCI | CCNGG | 6 cut(s) | 20, 273, 283, 515, 550, 705 |
| BstSFI | CTRYAG | 1 cut(s) | 30 |
| BstUI | CGCG | 3 cut(s) | 71, 86, 242 |
| BstV1I | GCAGC | 2 cut(s) | 318, 321 |
| BstV2I | GAAGAC | 1 cut(s) | 470 |
| BstX2I | RGATCY | 2 cut(s) | 328, 361 |
| BstYI | RGATCY | 2 cut(s) | 328, 361 |
| BsuRI | GGCC | 4 cut(s) | 278, 324, 476, 556 |
| BtgI | CCRYGG | 1 cut(s) | 240 |
| BtsCI | GGATG | 1 cut(s) | 219 |
| BtsIMutI | CAGTG | 2 cut(s) | 353, 578 |
| BveI | ACCTGC | 1 cut(s) | 562 |
| Cac8I | GCNNGC | 6 cut(s) | 27, 204, 216, 351, 484, 500 |
| CciI | TCATGA | 1 cut(s) | 438 |
| CfoI | GCGC | 3 cut(s) | 73, 88, 399 |
| Cfr10I | RCCGGY | 2 cut(s) | 536, 556 |
| Cfr13I | GGNCC | 3 cut(s) | 46, 554, 608 |
| Cfr42I | CCGCGG | 1 cut(s) | 243 |
| CpoI | CGGWCCG | 1 cut(s) | 46 |
| CseI | GACGC | 3 cut(s) | 30, 77, 475 |
| Csp6I | GTAC | 3 cut(s) | 343, 548, 575 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 576, 611 |
| CspI | CGGWCCG | 1 cut(s) | 46 |
| CviAII | CATG | 1 cut(s) | 439 |
| CviQI | GTAC | 3 cut(s) | 343, 548, 575 |
| DdeI | CTNAG | 2 cut(s) | 37, 591 |
| DpnI | GATC | 4 cut(s) | 59, 161, 330, 363 |
| DpnII | GATC | 4 cut(s) | 57, 159, 328, 361 |
| EaeI | YGGCCR | 1 cut(s) | 276 |
| Eam1104I | CTCTTC | 2 cut(s) | 173, 192 |
| EarI | CTCTTC | 2 cut(s) | 173, 192 |
| EciI | GGCGGA | 1 cut(s) | 374 |
| Eco47I | GGWCC | 2 cut(s) | 46, 608 |
| Eco57I | CTGAAG | 4 cut(s) | 144, 216, 432, 714 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 181 |
| EcoRII | CCWGG | 3 cut(s) | 20, 515, 550 |
| FaeI | CATG | 1 cut(s) | 442 |
| FaiI | YATR | 9 cut(s) | 130, 141, 180, 341, 369, 434, 440, 563, 620 |
| FaqI | GGGAC | 3 cut(s) | 121, 621, 689 |
| FatI | CATG | 1 cut(s) | 438 |
| FauI | CCCGC | 6 cut(s) | 18, 223, 247, 491, 666, 703 |
| FblI | GTMKAC | 1 cut(s) | 526 |
| Fnu4HI | GCNGC | 5 cut(s) | 63, 74, 289, 307, 310 |
| FokI | GGATG | 1 cut(s) | 206 |
| Fsp4HI | GCNGC | 5 cut(s) | 63, 74, 289, 307, 310 |
| FspBI | CTAG | 2 cut(s) | 350, 681 |
| GlaI | GCGC | 3 cut(s) | 72, 87, 398 |
| GluI | GCNGC | 5 cut(s) | 63, 74, 289, 307, 310 |
| GsaI | CCCAGC | 2 cut(s) | 491, 534 |
| GsuI | CTGGAG | 2 cut(s) | 38, 491 |
| HaeIII | GGCC | 4 cut(s) | 278, 324, 476, 556 |
| HapII | CCGG | 5 cut(s) | 275, 285, 537, 557, 707 |
| HgaI | GACGC | 3 cut(s) | 30, 77, 475 |
| HhaI | GCGC | 3 cut(s) | 73, 88, 399 |
| Hin1II | CATG | 1 cut(s) | 442 |
| Hin6I | GCGC | 3 cut(s) | 71, 86, 397 |
| HinP1I | GCGC | 3 cut(s) | 71, 86, 397 |
| HincII | GTYRAC | 2 cut(s) | 253, 527 |
| HindII | GTYRAC | 2 cut(s) | 253, 527 |
| HindIII | AAGCTT | 1 cut(s) | 104 |
| HinfI | GANTC | 1 cut(s) | 650 |
| HpaI | GTTAAC | 1 cut(s) | 253 |
| HpaII | CCGG | 5 cut(s) | 275, 285, 537, 557, 707 |
| HphI | GGTGA | 1 cut(s) | 166 |
| Hpy166II | GTNNAC | 2 cut(s) | 253, 527 |
| Hpy188I | TCNGA | 5 cut(s) | 46, 102, 111, 235, 676 |
| Hpy188III | TCNNGA | 2 cut(s) | 55, 439 |
| Hpy8I | GTNNAC | 2 cut(s) | 253, 527 |
| Hpy99I | CGWCG | 2 cut(s) | 46, 71 |
| HpyAV | CCTTC | 1 cut(s) | 689 |
| HpyCH4IV | ACGT | 3 cut(s) | 385, 445, 508 |
| HpyCH4V | TGCA | 1 cut(s) | 571 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 26, 68, 94, 103, 488 |
| HpyF3I | CTNAG | 2 cut(s) | 37, 591 |
| HpySE526I | ACGT | 3 cut(s) | 385, 445, 508 |
| Hsp92II | CATG | 1 cut(s) | 442 |
| HspAI | GCGC | 3 cut(s) | 71, 86, 397 |
| KpnI | GGTACC | 2 cut(s) | 551, 578 |
| KspAI | GTTAAC | 1 cut(s) | 253 |
| KspI | CCGCGG | 1 cut(s) | 243 |
| Kzo9I | GATC | 4 cut(s) | 57, 159, 328, 361 |
| LguI | GCTCTTC | 1 cut(s) | 192 |
| LmnI | GCTCC | 2 cut(s) | 86, 394 |
| Lsp1109I | GCAGC | 2 cut(s) | 318, 321 |
| LweI | GCATC | 2 cut(s) | 106, 558 |
| MaeI | CTAG | 2 cut(s) | 350, 681 |
| MaeII | ACGT | 3 cut(s) | 385, 445, 508 |
| MaeIII | GTNAC | 2 cut(s) | 334, 504 |
| MalI | GATC | 4 cut(s) | 59, 161, 330, 363 |
| MbiI | CCGCTC | 1 cut(s) | 672 |
| MboI | GATC | 4 cut(s) | 57, 159, 328, 361 |
| MboII | GAAGA | 5 cut(s) | 160, 169, 209, 356, 475 |
| MflI | RGATCY | 2 cut(s) | 328, 361 |
| MhlI | GDGCHC | 1 cut(s) | 262 |
| MmeI | TCCRAC | 2 cut(s) | 24, 258 |
| MseI | TTAA | 1 cut(s) | 252 |
| MslI | CAYNNNNRTG | 1 cut(s) | 566 |
| MspA1I | CMGCKG | 1 cut(s) | 242 |
| MspI | CCGG | 5 cut(s) | 275, 285, 537, 557, 707 |
| MspR9I | CCNGG | 6 cut(s) | 22, 275, 285, 517, 552, 707 |
| MvaI | CCWGG | 3 cut(s) | 22, 517, 552 |
| MvnI | CGCG | 3 cut(s) | 71, 86, 242 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 26, 68, 94, 103, 488 |
| NciI | CCSGG | 3 cut(s) | 275, 285, 707 |
| NdeII | GATC | 4 cut(s) | 57, 159, 328, 361 |
| NheI | GCTAGC | 1 cut(s) | 349 |
| NlaIII | CATG | 1 cut(s) | 442 |
| NlaIV | GGNNCC | 3 cut(s) | 549, 576, 609 |
| NmuCI | GTSAC | 2 cut(s) | 334, 504 |
| PagI | TCATGA | 1 cut(s) | 438 |
| PciSI | GCTCTTC | 1 cut(s) | 192 |
| PfeI | GAWTC | 1 cut(s) | 650 |
| PkrI | GCNGC | 5 cut(s) | 64, 75, 290, 308, 311 |
| Ppu21I | YACGTR | 1 cut(s) | 509 |
| Psp6I | CCWGG | 3 cut(s) | 20, 515, 550 |
| PspFI | CCCAGC | 2 cut(s) | 487, 530 |
| PspGI | CCWGG | 3 cut(s) | 20, 515, 550 |
| PspN4I | GGNNCC | 3 cut(s) | 549, 576, 609 |
| PspPI | GGNCC | 3 cut(s) | 46, 554, 608 |
| PsuI | RGATCY | 2 cut(s) | 328, 361 |
| RsaI | GTAC | 3 cut(s) | 344, 549, 576 |
| RsaNI | GTAC | 3 cut(s) | 343, 548, 575 |
| RseI | CAYNNNNRTG | 1 cut(s) | 566 |
| Rsr2I | CGGWCCG | 1 cut(s) | 46 |
| RsrII | CGGWCCG | 1 cut(s) | 46 |
| SacII | CCGCGG | 1 cut(s) | 243 |
| SalI | GTCGAC | 1 cut(s) | 525 |
| SapI | GCTCTTC | 1 cut(s) | 192 |
| SaqAI | TTAA | 1 cut(s) | 252 |
| SatI | GCNGC | 5 cut(s) | 63, 74, 289, 307, 310 |
| Sau3AI | GATC | 4 cut(s) | 57, 159, 328, 361 |
| Sau96I | GGNCC | 3 cut(s) | 46, 554, 608 |
| ScrFI | CCNGG | 6 cut(s) | 22, 275, 285, 517, 552, 707 |
| SduI | GDGCHC | 1 cut(s) | 262 |
| SetI | ASST | 9 cut(s) | 108, 168, 179, 351, 388, 448, 511, 576, 592 |
| SfaNI | GCATC | 2 cut(s) | 106, 558 |
| SfcI | CTRYAG | 1 cut(s) | 30 |
| Sfr303I | CCGCGG | 1 cut(s) | 243 |
| SgrBI | CCGCGG | 1 cut(s) | 243 |
| SinI | GGWCC | 2 cut(s) | 46, 608 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 566 |
| SspMI | CTAG | 2 cut(s) | 350, 681 |
| StyD4I | CCNGG | 6 cut(s) | 20, 273, 283, 515, 550, 705 |
| TaiI | ACGT | 3 cut(s) | 388, 448, 511 |
| TaqI | TCGA | 2 cut(s) | 144, 526 |
| TauI | GCSGC | 3 cut(s) | 65, 76, 291 |
| TfiI | GAWTC | 1 cut(s) | 650 |
| Tru1I | TTAA | 1 cut(s) | 252 |
| Tru9I | TTAA | 1 cut(s) | 252 |
| TscAI | CASTG | 2 cut(s) | 360, 585 |
| TseFI | GTSAC | 2 cut(s) | 334, 504 |
| TseI | GCWGC | 2 cut(s) | 306, 309 |
| Tsp45I | GTSAC | 2 cut(s) | 334, 504 |
| TspDTI | ATGAA | 1 cut(s) | 356 |
| TspRI | CASTG | 2 cut(s) | 360, 585 |
| VpaK11BI | GGWCC | 2 cut(s) | 46, 608 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 181 |
| XmiI | GTMKAC | 1 cut(s) | 526 |
| XspI | CTAG | 2 cut(s) | 350, 681 |