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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 23, 62, 76 |
| AclI | AACGTT | 1 cut(s) | 51 |
| AcuI | CTGAAG | 1 cut(s) | 23 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 109, 235 |
| AgsI | TTSAA | 1 cut(s) | 88 |
| AjnI | CCWGG | 2 cut(s) | 166, 184 |
| AluBI | AGCT | 1 cut(s) | 44 |
| AluI | AGCT | 1 cut(s) | 44 |
| AoxI | GGCC | 3 cut(s) | 187, 261, 267 |
| AspS9I | GGNCC | 3 cut(s) | 188, 193, 226 |
| AsuC2I | CCSGG | 1 cut(s) | 191 |
| AvaII | GGWCC | 2 cut(s) | 193, 226 |
| BccI | CCATC | 1 cut(s) | 208 |
| BciT130I | CCWGG | 2 cut(s) | 168, 186 |
| BcnI | CCSGG | 1 cut(s) | 191 |
| BfaI | CTAG | 1 cut(s) | 105 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 186 |
| Bme1390I | CCNGG | 3 cut(s) | 168, 186, 191 |
| Bme18I | GGWCC | 2 cut(s) | 193, 226 |
| BmgT120I | GGNCC | 3 cut(s) | 188, 193, 226 |
| BmiI | GGNNCC | 1 cut(s) | 114 |
| BmrFI | CCNGG | 3 cut(s) | 168, 186, 191 |
| BpmI | CTGGAG | 1 cut(s) | 215 |
| BpuMI | CCSGG | 1 cut(s) | 191 |
| BsaJI | CCNNGG | 1 cut(s) | 264 |
| BsaWI | WCCGGW | 1 cut(s) | 94 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 109, 235 |
| BseBI | CCWGG | 2 cut(s) | 168, 186 |
| BseDI | CCNNGG | 1 cut(s) | 264 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 109, 235 |
| BseYI | CCCAGC | 1 cut(s) | 58 |
| BshFI | GGCC | 3 cut(s) | 189, 263, 269 |
| BsiSI | CCGG | 2 cut(s) | 95, 191 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 109, 235 |
| BsnI | GGCC | 3 cut(s) | 189, 263, 269 |
| BspACI | CCGC | 3 cut(s) | 23, 62, 76 |
| BspANI | GGCC | 3 cut(s) | 189, 263, 269 |
| BspLI | GGNNCC | 1 cut(s) | 114 |
| BssECI | CCNNGG | 1 cut(s) | 264 |
| Bst2UI | CCWGG | 2 cut(s) | 168, 186 |
| Bst4CI | ACNGT | 1 cut(s) | 176 |
| BstC8I | GCNNGC | 1 cut(s) | 11 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 233 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 75, 177, 186 |
| BstNI | CCWGG | 2 cut(s) | 168, 186 |
| BstSCI | CCNGG | 3 cut(s) | 166, 184, 189 |
| BsuRI | GGCC | 3 cut(s) | 189, 263, 269 |
| Cac8I | GCNNGC | 1 cut(s) | 11 |
| Cfr13I | GGNCC | 3 cut(s) | 188, 193, 226 |
| CviJI | RGCY | 6 cut(s) | 44, 115, 161, 189, 263, 269 |
| CviKI_1 | RGCY | 6 cut(s) | 44, 115, 161, 189, 263, 269 |
| EciI | GGCGGA | 1 cut(s) | 12 |
| Eco147I | AGGCCT | 1 cut(s) | 269 |
| Eco47I | GGWCC | 2 cut(s) | 193, 226 |
| Eco57I | CTGAAG | 1 cut(s) | 23 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 233 |
| EcoO109I | RGGNCCY | 1 cut(s) | 226 |
| EcoRII | CCWGG | 2 cut(s) | 166, 184 |
| FaiI | YATR | 2 cut(s) | 206, 210 |
| FspBI | CTAG | 1 cut(s) | 105 |
| GsaI | CCCAGC | 1 cut(s) | 62 |
| GsuI | CTGGAG | 1 cut(s) | 215 |
| HaeIII | GGCC | 3 cut(s) | 189, 263, 269 |
| HapII | CCGG | 2 cut(s) | 95, 191 |
| HincII | GTYRAC | 2 cut(s) | 55, 73 |
| HindII | GTYRAC | 2 cut(s) | 55, 73 |
| HpaI | GTTAAC | 2 cut(s) | 55, 73 |
| HpaII | CCGG | 2 cut(s) | 95, 191 |
| Hpy166II | GTNNAC | 3 cut(s) | 55, 73, 100 |
| Hpy188III | TCNNGA | 1 cut(s) | 232 |
| Hpy8I | GTNNAC | 3 cut(s) | 55, 73, 100 |
| HpyAV | CCTTC | 1 cut(s) | 229 |
| HpyCH4III | ACNGT | 1 cut(s) | 176 |
| HpyCH4IV | ACGT | 1 cut(s) | 51 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 75, 177, 186 |
| HpySE526I | ACGT | 1 cut(s) | 51 |
| KspAI | GTTAAC | 2 cut(s) | 55, 73 |
| LmnI | GCTCC | 2 cut(s) | 112, 247 |
| LpnPI | CCDG | 9 cut(s) | 72, 108, 143, 153, 171, 180, 198, 204, 245 |
| MaeI | CTAG | 1 cut(s) | 105 |
| MaeII | ACGT | 1 cut(s) | 51 |
| MboII | GAAGA | 1 cut(s) | 16 |
| MnlI | CCTC | 4 cut(s) | 151, 217, 239, 259 |
| MseI | TTAA | 2 cut(s) | 54, 72 |
| MspA1I | CMGCKG | 1 cut(s) | 62 |
| MspI | CCGG | 2 cut(s) | 95, 191 |
| MspR9I | CCNGG | 3 cut(s) | 168, 186, 191 |
| MvaI | CCWGG | 2 cut(s) | 168, 186 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 75, 177, 186 |
| NciI | CCSGG | 1 cut(s) | 191 |
| NlaIV | GGNNCC | 1 cut(s) | 114 |
| PceI | AGGCCT | 1 cut(s) | 269 |
| PpuMI | RGGWCCY | 1 cut(s) | 226 |
| Psp1406I | AACGTT | 1 cut(s) | 51 |
| Psp5II | RGGWCCY | 1 cut(s) | 226 |
| Psp6I | CCWGG | 2 cut(s) | 166, 184 |
| PspFI | CCCAGC | 1 cut(s) | 58 |
| PspGI | CCWGG | 2 cut(s) | 166, 184 |
| PspN4I | GGNNCC | 1 cut(s) | 114 |
| PspPI | GGNCC | 3 cut(s) | 188, 193, 226 |
| PspPPI | RGGWCCY | 1 cut(s) | 226 |
| SaqAI | TTAA | 2 cut(s) | 54, 72 |
| Sau96I | GGNCC | 3 cut(s) | 188, 193, 226 |
| ScrFI | CCNGG | 3 cut(s) | 168, 186, 191 |
| SetI | ASST | 4 cut(s) | 46, 54, 198, 231 |
| SinI | GGWCC | 2 cut(s) | 193, 226 |
| SseBI | AGGCCT | 1 cut(s) | 269 |
| SsiI | CCGC | 3 cut(s) | 23, 62, 76 |
| SspMI | CTAG | 1 cut(s) | 105 |
| StuI | AGGCCT | 1 cut(s) | 269 |
| StyD4I | CCNGG | 3 cut(s) | 166, 184, 189 |
| TaaI | ACNGT | 1 cut(s) | 176 |
| TaiI | ACGT | 1 cut(s) | 54 |
| Tru1I | TTAA | 2 cut(s) | 54, 72 |
| Tru9I | TTAA | 2 cut(s) | 54, 72 |
| VpaK11BI | GGWCC | 2 cut(s) | 193, 226 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 233 |
| XspI | CTAG | 1 cut(s) | 105 |