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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 406 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 641 |
| AccBSI | CCGCTC | 2 cut(s) | 504, 716 |
| AccII | CGCG | 1 cut(s) | 90 |
| AclWI | GGATC | 2 cut(s) | 201, 668 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 2 cut(s) | 48, 264 |
| AcyI | GRCGYC | 1 cut(s) | 691 |
| AfaI | GTAC | 4 cut(s) | 176, 381, 396, 408 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 77, 151, 256, 368, 508, 641, 733 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 4 cut(s) | 347, 382, 550, 731 |
| AluBI | AGCT | 2 cut(s) | 181, 289 |
| AluI | AGCT | 2 cut(s) | 181, 289 |
| AlwI | GGATC | 2 cut(s) | 201, 668 |
| AoxI | GGCC | 6 cut(s) | 108, 154, 306, 386, 554, 735 |
| ApaI | GGGCCC | 2 cut(s) | 558, 739 |
| ApeKI | GCWGC | 4 cut(s) | 138, 141, 566, 747 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 3 cut(s) | 92, 231, 701 |
| AspS9I | GGNCC | 7 cut(s) | 154, 386, 440, 554, 555, 735, 736 |
| AsuC2I | CCSGG | 5 cut(s) | 107, 117, 153, 363, 678 |
| AsuHPI | GGTGA | 3 cut(s) | 134, 553, 734 |
| AsuNHI | GCTAGC | 1 cut(s) | 181 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| BaeGI | GKGCMC | 2 cut(s) | 558, 739 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 363, 390, 396, 423 |
| BanI | GGYRCC | 2 cut(s) | 379, 406 |
| BanII | GRGCYC | 2 cut(s) | 558, 739 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| BbvI | GCAGC | 4 cut(s) | 150, 153, 553, 734 |
| BccI | CCATC | 1 cut(s) | 59 |
| BceAI | ACGGC | 1 cut(s) | 640 |
| BciT130I | CCWGG | 4 cut(s) | 349, 384, 552, 733 |
| BcnI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 678 |
| BfaI | CTAG | 2 cut(s) | 182, 513 |
| BfmI | CTRYAG | 2 cut(s) | 575, 756 |
| BfoI | RGCGCY | 1 cut(s) | 702 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 363 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 5 cut(s) | 139, 142, 145, 567, 748 |
| BlsI | GCNGC | 5 cut(s) | 140, 143, 146, 568, 749 |
| Bme1390I | CCNGG | 9 cut(s) | 107, 117, 153, 349, 363, 384, 552, 678, 733 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmgT120I | GGNCC | 7 cut(s) | 154, 386, 440, 554, 555, 735, 736 |
| BmiI | GGNNCC | 5 cut(s) | 381, 408, 441, 556, 737 |
| BmrFI | CCNGG | 9 cut(s) | 107, 117, 153, 349, 363, 384, 552, 678, 733 |
| BmsI | GCATC | 1 cut(s) | 640 |
| BmtI | GCTAGC | 1 cut(s) | 185 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| BpmI | CTGGAG | 3 cut(s) | 182, 323, 681 |
| BpuMI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 678 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaHI | GRCGYC | 1 cut(s) | 691 |
| BsaJI | CCNNGG | 5 cut(s) | 105, 115, 383, 551, 732 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 654, 684 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 77, 151, 256, 368, 508, 641, 733 |
| Bse118I | RCCGGY | 2 cut(s) | 388, 589 |
| Bse1I | ACTGG | 2 cut(s) | 192, 664 |
| BseBI | CCWGG | 4 cut(s) | 349, 384, 552, 733 |
| BseDI | CCNNGG | 5 cut(s) | 105, 115, 383, 551, 732 |
| BseGI | GGATG | 2 cut(s) | 51, 655 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 77, 151, 256, 368, 508, 641, 733 |
| BseMII | CTCAG | 3 cut(s) | 414, 416, 516 |
| BseNI | ACTGG | 2 cut(s) | 192, 664 |
| BseRI | GAGGAG | 3 cut(s) | 236, 239, 299 |
| BseSI | GKGCMC | 2 cut(s) | 558, 739 |
| BseXI | GCAGC | 4 cut(s) | 150, 153, 553, 734 |
| BseYI | CCCAGC | 3 cut(s) | 70, 319, 695 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| Bsh1285I | CGRYCG | 1 cut(s) | 393 |
| BshFI | GGCC | 6 cut(s) | 110, 156, 308, 388, 556, 737 |
| BshNI | GGYRCC | 2 cut(s) | 379, 406 |
| BsiEI | CGRYCG | 1 cut(s) | 393 |
| BsiSI | CCGG | 7 cut(s) | 107, 117, 152, 363, 389, 590, 677 |
| BslFI | GGGAC | 2 cut(s) | 453, 678 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 77, 151, 256, 368, 508, 641, 733 |
| BsmFI | GGGAC | 2 cut(s) | 453, 678 |
| BsnI | GGCC | 6 cut(s) | 110, 156, 308, 388, 556, 737 |
| Bsp120I | GGGCCC | 2 cut(s) | 554, 735 |
| Bsp1286I | GDGCHC | 2 cut(s) | 558, 739 |
| Bsp143I | GATC | 3 cut(s) | 160, 193, 673 |
| BspANI | GGCC | 6 cut(s) | 110, 156, 308, 388, 556, 737 |
| BspCNI | CTCAG | 3 cut(s) | 415, 415, 517 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 5 cut(s) | 381, 408, 441, 556, 737 |
| BspMAI | CTGCAG | 2 cut(s) | 579, 760 |
| BspOI | GCTAGC | 1 cut(s) | 185 |
| BspPI | GGATC | 2 cut(s) | 201, 668 |
| BspQI | GCTCTTC | 1 cut(s) | 723 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 406 |
| BsrBI | CCGCTC | 2 cut(s) | 504, 716 |
| BsrFI | RCCGGY | 2 cut(s) | 388, 589 |
| BsrI | ACTGG | 2 cut(s) | 192, 664 |
| BssAI | RCCGGY | 2 cut(s) | 388, 589 |
| BssECI | CCNNGG | 5 cut(s) | 105, 115, 383, 551, 732 |
| BssMI | GATC | 3 cut(s) | 160, 193, 673 |
| BssNI | GRCGYC | 1 cut(s) | 691 |
| Bst2UI | CCWGG | 4 cut(s) | 349, 384, 552, 733 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 533 |
| Bst6I | CTCTTC | 1 cut(s) | 723 |
| BstACI | GRCGYC | 1 cut(s) | 691 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 6 cut(s) | 149, 183, 316, 332, 579, 760 |
| BstDEI | CTNAG | 3 cut(s) | 402, 423, 525 |
| BstF5I | GGATG | 2 cut(s) | 51, 655 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 1 cut(s) | 702 |
| BstHHI | GCGC | 3 cut(s) | 92, 231, 701 |
| BstKTI | GATC | 3 cut(s) | 163, 196, 676 |
| BstMBI | GATC | 3 cut(s) | 160, 193, 673 |
| BstMCI | CGRYCG | 1 cut(s) | 393 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 320, 363 |
| BstNI | CCWGG | 4 cut(s) | 349, 384, 552, 733 |
| BstSCI | CCNGG | 9 cut(s) | 105, 115, 151, 347, 361, 382, 550, 676, 731 |
| BstSFI | CTRYAG | 2 cut(s) | 575, 756 |
| BstSLI | GKGCMC | 2 cut(s) | 558, 739 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 4 cut(s) | 150, 153, 553, 734 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 6 cut(s) | 110, 156, 308, 388, 556, 737 |
| BtsCI | GGATG | 2 cut(s) | 51, 655 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 410 |
| Cac8I | GCNNGC | 6 cut(s) | 149, 183, 316, 332, 579, 760 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 3 cut(s) | 92, 231, 701 |
| Cfr10I | RCCGGY | 2 cut(s) | 388, 589 |
| Cfr13I | GGNCC | 7 cut(s) | 154, 386, 440, 554, 555, 735, 736 |
| CseI | GACGC | 2 cut(s) | 307, 680 |
| Csp6I | GTAC | 4 cut(s) | 175, 380, 395, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 1 cut(s) | 271 |
| CviQI | GTAC | 4 cut(s) | 175, 380, 395, 407 |
| DdeI | CTNAG | 3 cut(s) | 402, 423, 525 |
| DpnI | GATC | 3 cut(s) | 162, 195, 675 |
| DpnII | GATC | 3 cut(s) | 160, 193, 673 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 1 cut(s) | 723 |
| EarI | CTCTTC | 1 cut(s) | 723 |
| EciI | GGCGGA | 3 cut(s) | 206, 525, 696 |
| Eco24I | GRGCYC | 2 cut(s) | 558, 739 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 264 |
| EcoO109I | RGGNCCY | 2 cut(s) | 555, 736 |
| EcoRII | CCWGG | 4 cut(s) | 347, 382, 550, 731 |
| EcoT38I | GRGCYC | 2 cut(s) | 558, 739 |
| FaeI | CATG | 1 cut(s) | 274 |
| FaiI | YATR | 7 cut(s) | 173, 272, 452, 595, 597, 776, 778 |
| FaqI | GGGAC | 2 cut(s) | 453, 678 |
| FatI | CATG | 1 cut(s) | 270 |
| FauI | CCCGC | 3 cut(s) | 8, 156, 323 |
| FauNDI | CATATG | 2 cut(s) | 595, 776 |
| Fnu4HI | GCNGC | 5 cut(s) | 139, 142, 145, 567, 748 |
| FokI | GGATG | 2 cut(s) | 38, 662 |
| FriOI | GRGCYC | 2 cut(s) | 558, 739 |
| Fsp4HI | GCNGC | 5 cut(s) | 139, 142, 145, 567, 748 |
| FspBI | CTAG | 2 cut(s) | 182, 513 |
| GlaI | GCGC | 3 cut(s) | 91, 230, 700 |
| GluI | GCNGC | 5 cut(s) | 139, 142, 145, 567, 748 |
| GsaI | CCCAGC | 3 cut(s) | 74, 323, 699 |
| GsuI | CTGGAG | 3 cut(s) | 182, 323, 681 |
| HaeII | RGCGCY | 1 cut(s) | 702 |
| HaeIII | GGCC | 6 cut(s) | 110, 156, 308, 388, 556, 737 |
| HapII | CCGG | 7 cut(s) | 107, 117, 152, 363, 389, 590, 677 |
| HgaI | GACGC | 2 cut(s) | 307, 680 |
| HhaI | GCGC | 3 cut(s) | 92, 231, 701 |
| Hin1I | GRCGYC | 1 cut(s) | 691 |
| Hin1II | CATG | 1 cut(s) | 274 |
| Hin6I | GCGC | 3 cut(s) | 90, 229, 699 |
| HinP1I | GCGC | 3 cut(s) | 90, 229, 699 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 3 cut(s) | 400, 482, 684 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 7 cut(s) | 107, 117, 152, 363, 389, 590, 677 |
| HphI | GGTGA | 3 cut(s) | 134, 553, 734 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy188I | TCNGA | 5 cut(s) | 67, 508, 587, 768, 818 |
| Hpy188III | TCNNGA | 3 cut(s) | 199, 271, 727 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyAV | CCTTC | 3 cut(s) | 196, 568, 749 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 533 |
| HpyCH4IV | ACGT | 3 cut(s) | 217, 277, 340 |
| HpyCH4V | TGCA | 8 cut(s) | 544, 569, 577, 599, 653, 750, 758, 780 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 320, 363 |
| HpyF3I | CTNAG | 3 cut(s) | 402, 423, 525 |
| HpySE526I | ACGT | 3 cut(s) | 217, 277, 340 |
| Hsp92I | GRCGYC | 1 cut(s) | 691 |
| Hsp92II | CATG | 1 cut(s) | 274 |
| HspAI | GCGC | 3 cut(s) | 90, 229, 699 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 160, 193, 673 |
| LguI | GCTCTTC | 1 cut(s) | 723 |
| LmnI | GCTCC | 3 cut(s) | 226, 286, 662 |
| Lsp1109I | GCAGC | 4 cut(s) | 150, 153, 553, 734 |
| LweI | GCATC | 1 cut(s) | 640 |
| MaeI | CTAG | 2 cut(s) | 182, 513 |
| MaeII | ACGT | 3 cut(s) | 217, 277, 340 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 3 cut(s) | 162, 195, 675 |
| MbiI | CCGCTC | 2 cut(s) | 504, 716 |
| MboI | GATC | 3 cut(s) | 160, 193, 673 |
| MboII | GAAGA | 3 cut(s) | 41, 307, 710 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 2 cut(s) | 558, 739 |
| MlyI | GAGTC | 2 cut(s) | 394, 693 |
| MmeI | TCCRAC | 2 cut(s) | 90, 622 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 566, 747 |
| MspI | CCGG | 7 cut(s) | 107, 117, 152, 363, 389, 590, 677 |
| MspR9I | CCNGG | 9 cut(s) | 107, 117, 153, 349, 363, 384, 552, 678, 733 |
| MvaI | CCWGG | 4 cut(s) | 349, 384, 552, 733 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 320, 363 |
| NciI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 678 |
| NdeI | CATATG | 2 cut(s) | 595, 776 |
| NdeII | GATC | 3 cut(s) | 160, 193, 673 |
| NheI | GCTAGC | 1 cut(s) | 181 |
| NlaIII | CATG | 1 cut(s) | 274 |
| NlaIV | GGNNCC | 5 cut(s) | 381, 408, 441, 556, 737 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 1 cut(s) | 723 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 1 cut(s) | 482 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 641 |
| PkrI | GCNGC | 5 cut(s) | 140, 143, 146, 568, 749 |
| PleI | GAGTC | 2 cut(s) | 394, 692 |
| PpsI | GAGTC | 2 cut(s) | 394, 692 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| Psp6I | CCWGG | 4 cut(s) | 347, 382, 550, 731 |
| PspFI | CCCAGC | 3 cut(s) | 70, 319, 695 |
| PspGI | CCWGG | 4 cut(s) | 347, 382, 550, 731 |
| PspN4I | GGNNCC | 5 cut(s) | 381, 408, 441, 556, 737 |
| PspOMI | GGGCCC | 2 cut(s) | 554, 735 |
| PspPI | GGNCC | 7 cut(s) | 154, 386, 440, 554, 555, 735, 736 |
| PstI | CTGCAG | 2 cut(s) | 579, 760 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 4 cut(s) | 176, 381, 396, 408 |
| RsaNI | GTAC | 4 cut(s) | 175, 380, 395, 407 |
| SapI | GCTCTTC | 1 cut(s) | 723 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 5 cut(s) | 139, 142, 145, 567, 748 |
| Sau3AI | GATC | 3 cut(s) | 160, 193, 673 |
| Sau96I | GGNCC | 7 cut(s) | 154, 386, 440, 554, 555, 735, 736 |
| SbfI | CCTGCAGG | 2 cut(s) | 579, 760 |
| SchI | GAGTC | 2 cut(s) | 394, 693 |
| ScrFI | CCNGG | 9 cut(s) | 107, 117, 153, 349, 363, 384, 552, 678, 733 |
| SdaI | CCTGCAGG | 2 cut(s) | 579, 760 |
| SduI | GDGCHC | 2 cut(s) | 558, 739 |
| SfaNI | GCATC | 1 cut(s) | 640 |
| SfcI | CTRYAG | 2 cut(s) | 575, 756 |
| SinI | GGWCC | 1 cut(s) | 440 |
| Sse8387I | CCTGCAGG | 2 cut(s) | 579, 760 |
| SspMI | CTAG | 2 cut(s) | 182, 513 |
| StyD4I | CCNGG | 9 cut(s) | 105, 115, 151, 347, 361, 382, 550, 676, 731 |
| TaaI | ACNGT | 2 cut(s) | 11, 533 |
| TaiI | ACGT | 3 cut(s) | 220, 280, 343 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TfiI | GAWTC | 1 cut(s) | 482 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 192, 417 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 4 cut(s) | 138, 141, 566, 747 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspRI | CASTG | 2 cut(s) | 192, 417 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 641 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 2 cut(s) | 182, 513 |