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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 497, 669 |
| AccBSI | CCGCTC | 2 cut(s) | 523, 677 |
| AccII | CGCG | 2 cut(s) | 791, 827 |
| AcoI | YGGCCR | 1 cut(s) | 222 |
| AcsI | RAATTY | 1 cut(s) | 600 |
| AcyI | GRCGYC | 1 cut(s) | 498 |
| AdeI | CACNNNGTG | 1 cut(s) | 669 |
| AfaI | GTAC | 1 cut(s) | 292 |
| AgsI | TTSAA | 1 cut(s) | 652 |
| AjnI | CCWGG | 2 cut(s) | 533, 538 |
| AluBI | AGCT | 4 cut(s) | 142, 178, 394, 815 |
| AluI | AGCT | 4 cut(s) | 142, 178, 394, 815 |
| Alw21I | GWGCWC | 1 cut(s) | 180 |
| Alw26I | GTCTC | 1 cut(s) | 638 |
| AoxI | GGCC | 8 cut(s) | 201, 207, 222, 419, 485, 536, 542, 681 |
| ApaI | GGGCCC | 1 cut(s) | 423 |
| ApeKI | GCWGC | 1 cut(s) | 722 |
| ApoI | RAATTY | 1 cut(s) | 600 |
| AspA2I | CCTAGG | 1 cut(s) | 383 |
| AspLEI | GCGC | 6 cut(s) | 192, 400, 500, 508, 638, 829 |
| AsuHPI | GGTGA | 1 cut(s) | 541 |
| AvaII | GGWCC | 7 cut(s) | 40, 64, 88, 121, 154, 324, 749 |
| AvrII | CCTAGG | 1 cut(s) | 383 |
| AxyI | CCTNAGG | 1 cut(s) | 489 |
| BaeGI | GKGCMC | 1 cut(s) | 423 |
| BalI | TGGCCA | 1 cut(s) | 224 |
| BanI | GGYRCC | 2 cut(s) | 497, 669 |
| BanII | GRGCYC | 2 cut(s) | 180, 423 |
| Bbv12I | GWGCWC | 1 cut(s) | 180 |
| BbvI | GCAGC | 1 cut(s) | 734 |
| BccI | CCATC | 1 cut(s) | 311 |
| BceAI | ACGGC | 2 cut(s) | 554, 628 |
| BciT130I | CCWGG | 2 cut(s) | 535, 540 |
| BcoDI | GTCTC | 1 cut(s) | 638 |
| BfaI | CTAG | 5 cut(s) | 315, 384, 555, 666, 719 |
| BfoI | RGCGCY | 2 cut(s) | 501, 509 |
| BisI | GCNGC | 6 cut(s) | 129, 616, 678, 681, 723, 789 |
| BlnI | CCTAGG | 1 cut(s) | 383 |
| BlsI | GCNGC | 6 cut(s) | 130, 617, 679, 682, 724, 790 |
| Bme1390I | CCNGG | 2 cut(s) | 535, 540 |
| Bme18I | GGWCC | 7 cut(s) | 40, 64, 88, 121, 154, 324, 749 |
| BmiI | GGNNCC | 6 cut(s) | 325, 407, 420, 421, 499, 671 |
| BmrFI | CCNGG | 2 cut(s) | 535, 540 |
| BmsI | GCATC | 2 cut(s) | 421, 539 |
| BpmI | CTGGAG | 1 cut(s) | 284 |
| BsaHI | GRCGYC | 1 cut(s) | 498 |
| BsaJI | CCNNGG | 3 cut(s) | 204, 383, 539 |
| Bse1I | ACTGG | 1 cut(s) | 267 |
| Bse21I | CCTNAGG | 1 cut(s) | 489 |
| BseBI | CCWGG | 2 cut(s) | 535, 540 |
| BseDI | CCNNGG | 3 cut(s) | 204, 383, 539 |
| BseGI | GGATG | 3 cut(s) | 530, 778, 819 |
| BseMII | CTCAG | 2 cut(s) | 480, 701 |
| BseNI | ACTGG | 1 cut(s) | 267 |
| BseRI | GAGGAG | 1 cut(s) | 656 |
| BseSI | GKGCMC | 1 cut(s) | 423 |
| BseXI | GCAGC | 1 cut(s) | 734 |
| BseYI | CCCAGC | 1 cut(s) | 502 |
| Bsh1236I | CGCG | 2 cut(s) | 791, 827 |
| BshFI | GGCC | 8 cut(s) | 203, 209, 224, 421, 487, 538, 544, 683 |
| BshNI | GGYRCC | 2 cut(s) | 497, 669 |
| BsiHKAI | GWGCWC | 1 cut(s) | 180 |
| BsiSI | CCGG | 1 cut(s) | 484 |
| BsmAI | GTCTC | 1 cut(s) | 638 |
| BsnI | GGCC | 8 cut(s) | 203, 209, 224, 421, 487, 538, 544, 683 |
| Bsp120I | GGGCCC | 1 cut(s) | 419 |
| Bsp1286I | GDGCHC | 2 cut(s) | 180, 423 |
| BspANI | GGCC | 8 cut(s) | 203, 209, 224, 421, 487, 538, 544, 683 |
| BspCNI | CTCAG | 2 cut(s) | 481, 700 |
| BspFNI | CGCG | 2 cut(s) | 791, 827 |
| BspHI | TCATGA | 1 cut(s) | 804 |
| BspLI | GGNNCC | 6 cut(s) | 325, 407, 420, 421, 499, 671 |
| BspQI | GCTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| BspT107I | GGYRCC | 2 cut(s) | 497, 669 |
| BsrBI | CCGCTC | 2 cut(s) | 523, 677 |
| BsrI | ACTGG | 1 cut(s) | 267 |
| BssECI | CCNNGG | 3 cut(s) | 204, 383, 539 |
| BssNI | GRCGYC | 1 cut(s) | 498 |
| BssT1I | CCWWGG | 1 cut(s) | 383 |
| Bst2UI | CCWGG | 2 cut(s) | 535, 540 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| BstACI | GRCGYC | 1 cut(s) | 498 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 739 |
| BstDEI | CTNAG | 2 cut(s) | 489, 687 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 161, 492 |
| BstF5I | GGATG | 3 cut(s) | 530, 778, 819 |
| BstFNI | CGCG | 2 cut(s) | 791, 827 |
| BstH2I | RGCGCY | 2 cut(s) | 501, 509 |
| BstHHI | GCGC | 6 cut(s) | 192, 400, 500, 508, 638, 829 |
| BstMAI | GTCTC | 1 cut(s) | 638 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 422, 431, 484, 722, 739 |
| BstNI | CCWGG | 2 cut(s) | 535, 540 |
| BstNSI | RCATGY | 2 cut(s) | 380, 587 |
| BstSCI | CCNGG | 2 cut(s) | 533, 538 |
| BstSLI | GKGCMC | 1 cut(s) | 423 |
| BstUI | CGCG | 2 cut(s) | 791, 827 |
| BstV1I | GCAGC | 1 cut(s) | 734 |
| Bsu36I | CCTNAGG | 1 cut(s) | 489 |
| BsuRI | GGCC | 8 cut(s) | 203, 209, 224, 421, 487, 538, 544, 683 |
| BtgZI | GCGATG | 1 cut(s) | 444 |
| BtsCI | GGATG | 3 cut(s) | 530, 778, 819 |
| BtsIMutI | CAGTG | 1 cut(s) | 695 |
| CciI | TCATGA | 1 cut(s) | 804 |
| CfoI | GCGC | 6 cut(s) | 192, 400, 500, 508, 638, 829 |
| CseI | GACGC | 1 cut(s) | 601 |
| Csp6I | GTAC | 1 cut(s) | 291 |
| CviAII | CATG | 5 cut(s) | 377, 413, 584, 619, 805 |
| CviQI | GTAC | 1 cut(s) | 291 |
| DdeI | CTNAG | 2 cut(s) | 489, 687 |
| DinI | GGCGCC | 1 cut(s) | 499 |
| DraIII | CACNNNGTG | 1 cut(s) | 669 |
| EaeI | YGGCCR | 1 cut(s) | 222 |
| Eam1104I | CTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| EarI | CTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| EciI | GGCGGA | 9 cut(s) | 37, 53, 61, 77, 85, 101, 167, 378, 848 |
| Ecl136II | GAGCTC | 1 cut(s) | 178 |
| Eco130I | CCWWGG | 1 cut(s) | 383 |
| Eco24I | GRGCYC | 2 cut(s) | 180, 423 |
| Eco47I | GGWCC | 7 cut(s) | 40, 64, 88, 121, 154, 324, 749 |
| Eco53kI | GAGCTC | 1 cut(s) | 178 |
| Eco81I | CCTNAGG | 1 cut(s) | 489 |
| EcoICRI | GAGCTC | 1 cut(s) | 178 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 161, 492 |
| EcoO109I | RGGNCCY | 2 cut(s) | 121, 419 |
| EcoRII | CCWGG | 2 cut(s) | 533, 538 |
| EcoT14I | CCWWGG | 1 cut(s) | 383 |
| EcoT38I | GRGCYC | 2 cut(s) | 180, 423 |
| EgeI | GGCGCC | 1 cut(s) | 499 |
| EheI | GGCGCC | 1 cut(s) | 499 |
| ErhI | CCWWGG | 1 cut(s) | 383 |
| FaeI | CATG | 5 cut(s) | 380, 416, 587, 622, 808 |
| FatI | CATG | 5 cut(s) | 376, 412, 583, 618, 804 |
| FauI | CCCGC | 3 cut(s) | 381, 409, 430 |
| Fnu4HI | GCNGC | 6 cut(s) | 129, 616, 678, 681, 723, 789 |
| FokI | GGATG | 3 cut(s) | 517, 765, 806 |
| FriOI | GRGCYC | 2 cut(s) | 180, 423 |
| Fsp4HI | GCNGC | 6 cut(s) | 129, 616, 678, 681, 723, 789 |
| FspBI | CTAG | 5 cut(s) | 315, 384, 555, 666, 719 |
| GlaI | GCGC | 6 cut(s) | 191, 399, 499, 507, 637, 828 |
| GluI | GCNGC | 6 cut(s) | 129, 616, 678, 681, 723, 789 |
| GsaI | CCCAGC | 1 cut(s) | 506 |
| GsuI | CTGGAG | 1 cut(s) | 284 |
| HaeII | RGCGCY | 2 cut(s) | 501, 509 |
| HaeIII | GGCC | 8 cut(s) | 203, 209, 224, 421, 487, 538, 544, 683 |
| HapII | CCGG | 1 cut(s) | 484 |
| HgaI | GACGC | 1 cut(s) | 601 |
| HhaI | GCGC | 6 cut(s) | 192, 400, 500, 508, 638, 829 |
| Hin1I | GRCGYC | 1 cut(s) | 498 |
| Hin1II | CATG | 5 cut(s) | 380, 416, 587, 622, 808 |
| Hin6I | GCGC | 6 cut(s) | 190, 398, 498, 506, 636, 827 |
| HinP1I | GCGC | 6 cut(s) | 190, 398, 498, 506, 636, 827 |
| HinfI | GANTC | 1 cut(s) | 559 |
| HpaII | CCGG | 1 cut(s) | 484 |
| HphI | GGTGA | 1 cut(s) | 541 |
| Hpy166II | GTNNAC | 1 cut(s) | 327 |
| Hpy188III | TCNNGA | 2 cut(s) | 237, 805 |
| Hpy8I | GTNNAC | 1 cut(s) | 327 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4V | TGCA | 4 cut(s) | 434, 460, 565, 587 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 422, 431, 484, 722, 739 |
| HpyF3I | CTNAG | 2 cut(s) | 489, 687 |
| Hsp92I | GRCGYC | 1 cut(s) | 498 |
| Hsp92II | CATG | 5 cut(s) | 380, 416, 587, 622, 808 |
| HspAI | GCGC | 6 cut(s) | 190, 398, 498, 506, 636, 827 |
| KasI | GGCGCC | 1 cut(s) | 497 |
| LguI | GCTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| LmnI | GCTCC | 2 cut(s) | 175, 391 |
| Lsp1109I | GCAGC | 1 cut(s) | 734 |
| LweI | GCATC | 2 cut(s) | 421, 539 |
| MaeI | CTAG | 5 cut(s) | 315, 384, 555, 666, 719 |
| MbiI | CCGCTC | 2 cut(s) | 523, 677 |
| MboII | GAAGA | 5 cut(s) | 41, 65, 89, 113, 517 |
| MhlI | GDGCHC | 2 cut(s) | 180, 423 |
| MlsI | TGGCCA | 1 cut(s) | 224 |
| MluCI | AATT | 4 cut(s) | 464, 600, 607, 769 |
| MluNI | TGGCCA | 1 cut(s) | 224 |
| Mly113I | GGCGCC | 1 cut(s) | 498 |
| Mox20I | TGGCCA | 1 cut(s) | 224 |
| MscI | TGGCCA | 1 cut(s) | 224 |
| MseI | TTAA | 1 cut(s) | 729 |
| MslI | CAYNNNNRTG | 1 cut(s) | 230 |
| Msp20I | TGGCCA | 1 cut(s) | 224 |
| MspA1I | CMGCKG | 1 cut(s) | 425 |
| MspI | CCGG | 1 cut(s) | 484 |
| MspR9I | CCNGG | 2 cut(s) | 535, 540 |
| MvaI | CCWGG | 2 cut(s) | 535, 540 |
| MvnI | CGCG | 2 cut(s) | 791, 827 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 422, 431, 484, 722, 739 |
| NarI | GGCGCC | 1 cut(s) | 498 |
| NlaIII | CATG | 5 cut(s) | 380, 416, 587, 622, 808 |
| NlaIV | GGNNCC | 6 cut(s) | 325, 407, 420, 421, 499, 671 |
| NmeAIII | GCCGAG | 2 cut(s) | 229, 698 |
| NspI | RCATGY | 2 cut(s) | 380, 587 |
| PagI | TCATGA | 1 cut(s) | 804 |
| PciSI | GCTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| PfeI | GAWTC | 1 cut(s) | 559 |
| PflFI | GACNNNGTC | 1 cut(s) | 800 |
| PkrI | GCNGC | 6 cut(s) | 130, 617, 679, 682, 724, 790 |
| PluTI | GGCGCC | 1 cut(s) | 501 |
| PpuMI | RGGWCCY | 1 cut(s) | 121 |
| Psp124BI | GAGCTC | 1 cut(s) | 180 |
| Psp5II | RGGWCCY | 1 cut(s) | 121 |
| Psp6I | CCWGG | 2 cut(s) | 533, 538 |
| PspFI | CCCAGC | 1 cut(s) | 502 |
| PspGI | CCWGG | 2 cut(s) | 533, 538 |
| PspN4I | GGNNCC | 6 cut(s) | 325, 407, 420, 421, 499, 671 |
| PspOMI | GGGCCC | 1 cut(s) | 419 |
| PspPPI | RGGWCCY | 1 cut(s) | 121 |
| PsyI | GACNNNGTC | 1 cut(s) | 800 |
| RsaI | GTAC | 1 cut(s) | 292 |
| RsaNI | GTAC | 1 cut(s) | 291 |
| RseI | CAYNNNNRTG | 1 cut(s) | 230 |
| SacI | GAGCTC | 1 cut(s) | 180 |
| SapI | GCTCTTC | 5 cut(s) | 24, 48, 72, 96, 530 |
| SaqAI | TTAA | 1 cut(s) | 729 |
| SatI | GCNGC | 6 cut(s) | 129, 616, 678, 681, 723, 789 |
| ScrFI | CCNGG | 2 cut(s) | 535, 540 |
| SduI | GDGCHC | 2 cut(s) | 180, 423 |
| SfaNI | GCATC | 2 cut(s) | 421, 539 |
| SfoI | GGCGCC | 1 cut(s) | 499 |
| SinI | GGWCC | 7 cut(s) | 40, 64, 88, 121, 154, 324, 749 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 230 |
| Sse9I | AATT | 4 cut(s) | 464, 600, 607, 769 |
| SspDI | GGCGCC | 1 cut(s) | 497 |
| SspMI | CTAG | 5 cut(s) | 315, 384, 555, 666, 719 |
| SstI | GAGCTC | 1 cut(s) | 180 |
| StyD4I | CCNGG | 2 cut(s) | 533, 538 |
| StyI | CCWWGG | 1 cut(s) | 383 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 180 |
| TasI | AATT | 4 cut(s) | 464, 600, 607, 769 |
| TauI | GCSGC | 5 cut(s) | 131, 618, 680, 683, 791 |
| TfiI | GAWTC | 1 cut(s) | 559 |
| Tru1I | TTAA | 1 cut(s) | 729 |
| Tru9I | TTAA | 1 cut(s) | 729 |
| TscAI | CASTG | 1 cut(s) | 695 |
| TseI | GCWGC | 1 cut(s) | 722 |
| TspDTI | ATGAA | 1 cut(s) | 829 |
| TspRI | CASTG | 1 cut(s) | 695 |
| Tth111I | GACNNNGTC | 1 cut(s) | 800 |
| VpaK11BI | GGWCC | 7 cut(s) | 40, 64, 88, 121, 154, 324, 749 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 161, 492 |
| XapI | RAATTY | 1 cut(s) | 600 |
| XceI | RCATGY | 2 cut(s) | 380, 587 |
| XmaJI | CCTAGG | 1 cut(s) | 383 |
| XspI | CTAG | 5 cut(s) | 315, 384, 555, 666, 719 |