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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 9 cut(s) | 15, 48, 122, 167, 184, 197, 216, 239, 386 |
| AclWI | GGATC | 1 cut(s) | 249 |
| AcoI | YGGCCR | 1 cut(s) | 309 |
| AcyI | GRCGYC | 1 cut(s) | 294 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 121, 163, 392 |
| AgsI | TTSAA | 1 cut(s) | 364 |
| AjnI | CCWGG | 2 cut(s) | 178, 204 |
| AluBI | AGCT | 1 cut(s) | 229 |
| AluI | AGCT | 1 cut(s) | 229 |
| AlwI | GGATC | 1 cut(s) | 249 |
| AoxI | GGCC | 3 cut(s) | 181, 202, 309 |
| AspS9I | GGNCC | 3 cut(s) | 40, 208, 342 |
| AsuC2I | CCSGG | 1 cut(s) | 117 |
| AsuHPI | GGTGA | 2 cut(s) | 167, 182 |
| AsuNHI | GCTAGC | 1 cut(s) | 229 |
| AvaII | GGWCC | 3 cut(s) | 40, 208, 342 |
| BalI | TGGCCA | 1 cut(s) | 311 |
| BccI | CCATC | 1 cut(s) | 89 |
| BciT130I | CCWGG | 2 cut(s) | 180, 206 |
| BcnI | CCSGG | 1 cut(s) | 117 |
| BfaI | CTAG | 4 cut(s) | 200, 212, 230, 236 |
| BisI | GCNGC | 2 cut(s) | 184, 216 |
| BlsI | GCNGC | 2 cut(s) | 185, 217 |
| Bme1390I | CCNGG | 3 cut(s) | 117, 180, 206 |
| Bme18I | GGWCC | 3 cut(s) | 40, 208, 342 |
| BmgT120I | GGNCC | 3 cut(s) | 40, 208, 342 |
| BmiI | GGNNCC | 2 cut(s) | 126, 343 |
| BmrFI | CCNGG | 3 cut(s) | 117, 180, 206 |
| BmtI | GCTAGC | 1 cut(s) | 233 |
| Bpu10I | CCTNAGC | 1 cut(s) | 389 |
| BpuMI | CCSGG | 1 cut(s) | 117 |
| BsaHI | GRCGYC | 1 cut(s) | 294 |
| BsaJI | CCNNGG | 3 cut(s) | 115, 178, 369 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 121, 163, 392 |
| Bse1I | ACTGG | 1 cut(s) | 157 |
| BseBI | CCWGG | 2 cut(s) | 180, 206 |
| BseDI | CCNNGG | 3 cut(s) | 115, 178, 369 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 121, 163, 392 |
| BseNI | ACTGG | 1 cut(s) | 157 |
| BshFI | GGCC | 3 cut(s) | 183, 204, 311 |
| BsiSI | CCGG | 1 cut(s) | 117 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 121, 163, 392 |
| BsnI | GGCC | 3 cut(s) | 183, 204, 311 |
| Bsp143I | GATC | 1 cut(s) | 241 |
| BspACI | CCGC | 9 cut(s) | 15, 48, 122, 167, 184, 197, 216, 239, 386 |
| BspANI | GGCC | 3 cut(s) | 183, 204, 311 |
| BspLI | GGNNCC | 2 cut(s) | 126, 343 |
| BspOI | GCTAGC | 1 cut(s) | 233 |
| BspPI | GGATC | 1 cut(s) | 249 |
| BsrI | ACTGG | 1 cut(s) | 157 |
| BssECI | CCNNGG | 3 cut(s) | 115, 178, 369 |
| BssMI | GATC | 1 cut(s) | 241 |
| BssNI | GRCGYC | 1 cut(s) | 294 |
| BssT1I | CCWWGG | 1 cut(s) | 369 |
| Bst2UI | CCWGG | 2 cut(s) | 180, 206 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstACI | GRCGYC | 1 cut(s) | 294 |
| BstC8I | GCNNGC | 1 cut(s) | 231 |
| BstDEI | CTNAG | 1 cut(s) | 389 |
| BstEII | GGTNACC | 1 cut(s) | 76 |
| BstKTI | GATC | 1 cut(s) | 244 |
| BstMBI | GATC | 1 cut(s) | 241 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 96 |
| BstNI | CCWGG | 2 cut(s) | 180, 206 |
| BstNSI | RCATGY | 1 cut(s) | 38 |
| BstPI | GGTNACC | 1 cut(s) | 76 |
| BstSCI | CCNGG | 3 cut(s) | 115, 178, 204 |
| BstX2I | RGATCY | 1 cut(s) | 241 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 241 |
| BsuRI | GGCC | 3 cut(s) | 183, 204, 311 |
| BtsIMutI | CAGTG | 1 cut(s) | 314 |
| Cac8I | GCNNGC | 1 cut(s) | 231 |
| Cfr13I | GGNCC | 3 cut(s) | 40, 208, 342 |
| CseI | GACGC | 1 cut(s) | 302 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 334, 369 |
| CviAII | CATG | 1 cut(s) | 35 |
| CviJI | RGCY | 5 cut(s) | 183, 204, 215, 229, 311 |
| CviKI_1 | RGCY | 5 cut(s) | 183, 204, 215, 229, 311 |
| DdeI | CTNAG | 1 cut(s) | 389 |
| DpnI | GATC | 1 cut(s) | 243 |
| DpnII | GATC | 1 cut(s) | 241 |
| EaeI | YGGCCR | 1 cut(s) | 309 |
| EciI | GGCGGA | 1 cut(s) | 182 |
| Eco130I | CCWWGG | 1 cut(s) | 369 |
| Eco47I | GGWCC | 3 cut(s) | 40, 208, 342 |
| Eco91I | GGTNACC | 1 cut(s) | 76 |
| EcoO109I | RGGNCCY | 1 cut(s) | 208 |
| EcoO65I | GGTNACC | 1 cut(s) | 76 |
| EcoRII | CCWGG | 2 cut(s) | 178, 204 |
| EcoT14I | CCWWGG | 1 cut(s) | 369 |
| ErhI | CCWWGG | 1 cut(s) | 369 |
| FaeI | CATG | 1 cut(s) | 38 |
| FaiI | YATR | 4 cut(s) | 36, 153, 225, 249 |
| FatI | CATG | 1 cut(s) | 34 |
| FauI | CCCGC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 2 cut(s) | 184, 216 |
| Fsp4HI | GCNGC | 2 cut(s) | 184, 216 |
| FspBI | CTAG | 4 cut(s) | 200, 212, 230, 236 |
| GluI | GCNGC | 2 cut(s) | 184, 216 |
| HaeIII | GGCC | 3 cut(s) | 183, 204, 311 |
| HapII | CCGG | 1 cut(s) | 117 |
| HgaI | GACGC | 1 cut(s) | 302 |
| Hin1I | GRCGYC | 1 cut(s) | 294 |
| Hin1II | CATG | 1 cut(s) | 38 |
| HincII | GTYRAC | 2 cut(s) | 112, 324 |
| HindII | GTYRAC | 2 cut(s) | 112, 324 |
| HpaII | CCGG | 1 cut(s) | 117 |
| HphI | GGTGA | 2 cut(s) | 167, 182 |
| Hpy166II | GTNNAC | 4 cut(s) | 40, 112, 324, 345 |
| Hpy8I | GTNNAC | 4 cut(s) | 40, 112, 324, 345 |
| Hpy99I | CGWCG | 1 cut(s) | 32 |
| HpyAV | CCTTC | 1 cut(s) | 280 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4V | TGCA | 1 cut(s) | 99 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 96 |
| HpyF3I | CTNAG | 1 cut(s) | 389 |
| Hsp92I | GRCGYC | 1 cut(s) | 294 |
| Hsp92II | CATG | 1 cut(s) | 38 |
| Kzo9I | GATC | 1 cut(s) | 241 |
| LpnPI | CCDG | 9 cut(s) | 84, 120, 130, 165, 170, 191, 192, 218, 311 |
| MaeI | CTAG | 4 cut(s) | 200, 212, 230, 236 |
| MaeIII | GTNAC | 3 cut(s) | 76, 133, 188 |
| MalI | GATC | 1 cut(s) | 243 |
| MboI | GATC | 1 cut(s) | 241 |
| MfeI | CAATTG | 1 cut(s) | 85 |
| MflI | RGATCY | 1 cut(s) | 241 |
| MlsI | TGGCCA | 1 cut(s) | 311 |
| MluCI | AATT | 3 cut(s) | 85, 144, 376 |
| MluNI | TGGCCA | 1 cut(s) | 311 |
| MnlI | CCTC | 1 cut(s) | 150 |
| Mox20I | TGGCCA | 1 cut(s) | 311 |
| MscI | TGGCCA | 1 cut(s) | 311 |
| MslI | CAYNNNNRTG | 1 cut(s) | 317 |
| Msp20I | TGGCCA | 1 cut(s) | 311 |
| MspI | CCGG | 1 cut(s) | 117 |
| MspR9I | CCNGG | 3 cut(s) | 117, 180, 206 |
| MunI | CAATTG | 1 cut(s) | 85 |
| MvaI | CCWGG | 2 cut(s) | 180, 206 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 96 |
| NciI | CCSGG | 1 cut(s) | 117 |
| NdeII | GATC | 1 cut(s) | 241 |
| NheI | GCTAGC | 1 cut(s) | 229 |
| NlaIII | CATG | 1 cut(s) | 38 |
| NlaIV | GGNNCC | 2 cut(s) | 126, 343 |
| NmuCI | GTSAC | 1 cut(s) | 188 |
| NspI | RCATGY | 1 cut(s) | 38 |
| PkrI | GCNGC | 2 cut(s) | 185, 217 |
| PpuMI | RGGWCCY | 1 cut(s) | 208 |
| Psp5II | RGGWCCY | 1 cut(s) | 208 |
| Psp6I | CCWGG | 2 cut(s) | 178, 204 |
| PspEI | GGTNACC | 1 cut(s) | 76 |
| PspGI | CCWGG | 2 cut(s) | 178, 204 |
| PspN4I | GGNNCC | 2 cut(s) | 126, 343 |
| PspPI | GGNCC | 3 cut(s) | 40, 208, 342 |
| PspPPI | RGGWCCY | 1 cut(s) | 208 |
| PsuI | RGATCY | 1 cut(s) | 241 |
| RseI | CAYNNNNRTG | 1 cut(s) | 317 |
| SatI | GCNGC | 2 cut(s) | 184, 216 |
| Sau3AI | GATC | 1 cut(s) | 241 |
| Sau96I | GGNCC | 3 cut(s) | 40, 208, 342 |
| ScrFI | CCNGG | 3 cut(s) | 117, 180, 206 |
| SetI | ASST | 2 cut(s) | 213, 231 |
| SinI | GGWCC | 3 cut(s) | 40, 208, 342 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 317 |
| Sse9I | AATT | 3 cut(s) | 85, 144, 376 |
| SsiI | CCGC | 9 cut(s) | 15, 48, 122, 167, 184, 197, 216, 239, 386 |
| SspMI | CTAG | 4 cut(s) | 200, 212, 230, 236 |
| StyD4I | CCNGG | 3 cut(s) | 115, 178, 204 |
| StyI | CCWWGG | 1 cut(s) | 369 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TasI | AATT | 3 cut(s) | 85, 144, 376 |
| TauI | GCSGC | 2 cut(s) | 186, 218 |
| TscAI | CASTG | 1 cut(s) | 321 |
| TseFI | GTSAC | 1 cut(s) | 188 |
| Tsp45I | GTSAC | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 321 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 208, 342 |
| XceI | RCATGY | 1 cut(s) | 38 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 70 |
| XspI | CTAG | 4 cut(s) | 200, 212, 230, 236 |